Nucleosome assembly protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

nucleosome assembly protein (NAP), putative

Species:

Leishmania braziliensis

UniProt:

E9AII4_LEIBR

TriTrypDb:

LbrM.20.5510 , LBRM2903_200070000

Length:

281

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 5

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 8

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	12
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0043229	intracellular organelle	3	12
GO:0043231	intracellular membrane-bounded organelle	4	12
GO:0110165	cellular anatomical entity	1	12
GO:0000785	chromatin	2	1
GO:0005737	cytoplasm	2	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1
GO:0097014	ciliary plasm	5	1
GO:0099568	cytoplasmic region	3	1

Expansion

Sequence features

E9AII4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AII4

Function

Biological processes
Term	Name	Level	Count
GO:0006325	chromatin organization	4	12
GO:0006334	nucleosome assembly	7	12
GO:0006338	chromatin remodeling	5	12
GO:0009987	cellular process	1	12
GO:0016043	cellular component organization	3	12
GO:0022607	cellular component assembly	4	12
GO:0034728	nucleosome organization	6	12
GO:0043933	protein-containing complex organization	4	12
GO:0065003	protein-containing complex assembly	5	12
GO:0065004	protein-DNA complex assembly	6	12
GO:0071824	protein-DNA complex subunit organization	5	12
GO:0071840	cellular component organization or biogenesis	2	12

Molecular functions
Term	Name	Level	Count
GO:0003682	chromatin binding	2	1
GO:0005488	binding	1	1
GO:0005515	protein binding	2	1
GO:0042393	histone binding	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	127	131	PF00656	0.512
CLV_C14_Caspase3-7	152	156	PF00656	0.534
CLV_C14_Caspase3-7	9	13	PF00656	0.510
CLV_NRD_NRD_1	237	239	PF00675	0.452
CLV_NRD_NRD_1	32	34	PF00675	0.513
CLV_NRD_NRD_1	40	42	PF00675	0.553
CLV_PCSK_KEX2_1	177	179	PF00082	0.306
CLV_PCSK_KEX2_1	31	33	PF00082	0.428
CLV_PCSK_KEX2_1	40	42	PF00082	0.476
CLV_PCSK_PC1ET2_1	177	179	PF00082	0.306
CLV_PCSK_PC1ET2_1	31	33	PF00082	0.394
CLV_PCSK_SKI1_1	238	242	PF00082	0.440
CLV_PCSK_SKI1_1	276	280	PF00082	0.695
DEG_APCC_DBOX_1	237	245	PF00400	0.465
DEG_Nend_UBRbox_2	1	3	PF02207	0.633
DEG_SPOP_SBC_1	190	194	PF00917	0.489
DOC_MAPK_gen_1	102	110	PF00069	0.570
DOC_MAPK_MEF2A_6	102	110	PF00069	0.585
DOC_PP4_FxxP_1	79	82	PF00568	0.585
DOC_USP7_MATH_1	179	183	PF00917	0.551
DOC_USP7_MATH_1	190	194	PF00917	0.510
DOC_WW_Pin1_4	73	78	PF00397	0.665
LIG_14-3-3_CanoR_1	105	109	PF00244	0.524
LIG_APCC_ABBA_1	200	205	PF00400	0.516
LIG_APCC_ABBA_1	206	211	PF00400	0.494
LIG_APCC_ABBA_1	247	252	PF00400	0.391
LIG_APCC_ABBA_1	80	85	PF00400	0.585
LIG_BIR_III_2	171	175	PF00653	0.426
LIG_deltaCOP1_diTrp_1	143	153	PF00928	0.585
LIG_EH_1	112	116	PF12763	0.492
LIG_FHA_2	150	156	PF00498	0.604
LIG_FHA_2	165	171	PF00498	0.520
LIG_FHA_2	18	24	PF00498	0.454
LIG_FHA_2	7	13	PF00498	0.435
LIG_FHA_2	88	94	PF00498	0.560
LIG_LIR_Apic_2	76	82	PF02991	0.381
LIG_LIR_Gen_1	17	27	PF02991	0.456
LIG_LIR_Gen_1	201	210	PF02991	0.496
LIG_LIR_Nem_3	17	22	PF02991	0.459
LIG_LIR_Nem_3	23	29	PF02991	0.454
LIG_PTB_Apo_2	110	117	PF02174	0.492
LIG_SH2_NCK_1	19	23	PF00017	0.458
LIG_SH2_STAP1	83	87	PF00017	0.515
LIG_SH2_STAT5	19	22	PF00017	0.603
LIG_SH2_STAT5	91	94	PF00017	0.497
LIG_SH3_3	204	210	PF00018	0.548
LIG_SUMO_SIM_anti_2	243	249	PF11976	0.478
LIG_SUMO_SIM_anti_2	93	99	PF11976	0.528
LIG_SUMO_SIM_par_1	198	205	PF11976	0.585
LIG_TRAF2_1	133	136	PF00917	0.605
LIG_TRAF2_1	227	230	PF00917	0.638
LIG_UBA3_1	62	69	PF00899	0.546
MOD_CK1_1	149	155	PF00069	0.481
MOD_CK1_1	186	192	PF00069	0.554
MOD_CK1_1	73	79	PF00069	0.583
MOD_CK2_1	130	136	PF00069	0.582
MOD_CK2_1	17	23	PF00069	0.383
MOD_CK2_1	195	201	PF00069	0.532
MOD_CK2_1	240	246	PF00069	0.455
MOD_CK2_1	87	93	PF00069	0.581
MOD_GlcNHglycan	148	151	PF01048	0.292
MOD_GlcNHglycan	185	188	PF01048	0.356
MOD_GlcNHglycan	254	257	PF01048	0.686
MOD_GSK3_1	179	186	PF00069	0.521
MOD_GSK3_1	191	198	PF00069	0.516
MOD_GSK3_1	6	13	PF00069	0.576
MOD_NEK2_1	191	196	PF00069	0.502
MOD_NEK2_1	240	245	PF00069	0.404
MOD_NEK2_1	87	92	PF00069	0.584
MOD_PIKK_1	191	197	PF00454	0.585
MOD_PIKK_1	61	67	PF00454	0.429
MOD_PKA_2	104	110	PF00069	0.524
MOD_PKA_2	146	152	PF00069	0.463
MOD_PKA_2	179	185	PF00069	0.551
MOD_PKA_2	70	76	PF00069	0.652
MOD_Plk_2-3	130	136	PF00069	0.426
MOD_Plk_2-3	164	170	PF00069	0.471
MOD_Plk_2-3	17	23	PF00069	0.496
MOD_Plk_4	195	201	PF00069	0.548
MOD_Plk_4	240	246	PF00069	0.391
MOD_Plk_4	87	93	PF00069	0.559
MOD_ProDKin_1	73	79	PF00069	0.651
MOD_SUMO_for_1	49	52	PF00179	0.578
MOD_SUMO_rev_2	163	167	PF00179	0.551
MOD_SUMO_rev_2	232	241	PF00179	0.508
TRG_DiLeu_BaEn_4	124	130	PF01217	0.426
TRG_ENDOCYTIC_2	19	22	PF00928	0.458
TRG_ER_diLys_1	277	281	PF00400	0.674
TRG_NES_CRM1_1	52	65	PF08389	0.299
TRG_NLS_MonoCore_2	176	181	PF00514	0.551
TRG_NLS_MonoExtN_4	174	181	PF00514	0.614
TRG_NLS_MonoExtN_4	28	35	PF00514	0.333

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PCY5	Leptomonas seymouri	62%	98%
A0A0S4J5N0	Bodo saltans	34%	100%
A0A1X0NW78	Trypanosomatidae	33%	81%
A0A3Q8IAW7	Leishmania donovani	78%	100%
A0A3R7M0B2	Trypanosoma rangeli	41%	94%
A4HYX2	Leishmania infantum	78%	100%
C9ZIF4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	30%	89%
E9AUR9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	77%	100%
Q4QCR7	Leishmania major	77%	100%
V5D5V0	Trypanosoma cruzi	40%	92%