Putative calpain-like cysteine peptidase

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

calpain-like cysteine peptidase, putative

Species:

Leishmania braziliensis

UniProt:

E9AII3_LEIBR

TriTrypDb:

LbrM.20.5500 , LBRM2903_200069900

Length:

151

Annotations

LeishMANIAdb interaction annotations

by homology
Contact email: avilan@ula.ve
Publication title: Plasminogen binding proteins in secreted membrane vesicles of Leishmania mexicana
Publication 1st author(s): Figuera
Publication Identifier(s): 23178693
Host organism: -1
Interaction detection method(s): enzymatic linked imunosorbent assay
Interaction type: direct interaction
Identification method participant A: identification by antibody
Identification method participant B: identification by antibody
ID(s) interactor A: E9AUS1
ID(s) interactor B: P00747
Taxid interactor A: Leishmania mexicana
Taxid interactor B: Homo sapiens
Biological role(s) interactor A: unspecified role
Biological role(s) interactor B: unspecified role
Experimental role(s) interactor A: bait

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 2
Forrest at al. (metacyclic)	no	yes: 6
Forrest at al. (procyclic)	no	yes: 8
Silverman et al.	no	yes: 1
Pissara et al.	yes	yes: 27

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 15

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 24

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 46
NetGPI	no	yes: 0, no: 46

Cellular components
Term	Name	Level	Count
GO:0005654	nucleoplasm	2	1
GO:0005737	cytoplasm	2	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1
GO:0097014	ciliary plasm	5	1
GO:0097542	ciliary tip	2	1
GO:0099568	cytoplasmic region	3	1
GO:0110165	cellular anatomical entity	1	3
GO:0016020	membrane	2	2
GO:0020018	ciliary pocket membrane	6	1
GO:0031090	organelle membrane	3	2
GO:0031253	cell projection membrane	4	2
GO:0060170	ciliary membrane	5	2
GO:0098588	bounding membrane of organelle	4	2
GO:0098590	plasma membrane region	3	2

Expansion

Sequence features

E9AII3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AII3

PDB structure(s): 1r75_A , 2fe0_A

Function

Biological processes
Term	Name	Level	Count
GO:0001539	cilium or flagellum-dependent cell motility	3	1
GO:0009987	cellular process	1	2
GO:0048870	cell motility	2	1
GO:0060285	cilium-dependent cell motility	4	1
GO:0006996	organelle organization	4	1
GO:0016043	cellular component organization	3	1
GO:0022607	cellular component assembly	4	1
GO:0030030	cell projection organization	4	1
GO:0030031	cell projection assembly	5	1
GO:0044000	movement in host	4	1
GO:0044403	biological process involved in symbiotic interaction	2	1
GO:0044409	entry into host	5	1
GO:0044419	biological process involved in interspecies interaction between organisms	1	1
GO:0044782	cilium organization	5	1
GO:0051701	biological process involved in interaction with host	3	1
GO:0060271	cilium assembly	6	1
GO:0070925	organelle assembly	5	1
GO:0071840	cellular component organization or biogenesis	2	1
GO:0120031	plasma membrane bounded cell projection assembly	6	1
GO:0120036	plasma membrane bounded cell projection organization	5	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	1
GO:0016787	hydrolase activity	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	81	85	PF00656	0.293
CLV_NRD_NRD_1	125	127	PF00675	0.543
CLV_PCSK_KEX2_1	125	127	PF00082	0.559
CLV_PCSK_SKI1_1	131	135	PF00082	0.716
DOC_MAPK_gen_1	54	62	PF00069	0.430
DOC_MAPK_MEF2A_6	55	64	PF00069	0.388
DOC_PP1_RVXF_1	59	65	PF00149	0.392
DOC_USP7_UBL2_3	123	127	PF12436	0.621
DOC_USP7_UBL2_3	130	134	PF12436	0.643
LIG_APCC_ABBA_1	26	31	PF00400	0.558
LIG_APCC_ABBAyCdc20_2	25	31	PF00400	0.257
LIG_FHA_1	108	114	PF00498	0.452
LIG_LIR_Apic_2	11	17	PF02991	0.703
LIG_LIR_Nem_3	44	50	PF02991	0.421
LIG_LYPXL_S_1	93	97	PF13949	0.315
LIG_LYPXL_yS_3	94	97	PF13949	0.315
LIG_PCNA_yPIPBox_3	32	40	PF02747	0.385
LIG_SH2_CRK	14	18	PF00017	0.725
LIG_SH2_STAP1	56	60	PF00017	0.328
LIG_SH2_STAT5	78	81	PF00017	0.401
LIG_UBA3_1	35	43	PF00899	0.306
MOD_CK2_1	18	24	PF00069	0.238
MOD_N-GLC_1	18	23	PF02516	0.378
MOD_NEK2_1	39	44	PF00069	0.339
MOD_NEK2_1	74	79	PF00069	0.369
MOD_NMyristoyl	1	7	PF02799	0.676
MOD_Plk_4	108	114	PF00069	0.237
MOD_Plk_4	74	80	PF00069	0.349
MOD_Plk_4	88	94	PF00069	0.342
MOD_SUMO_rev_2	124	133	PF00179	0.670
MOD_SUMO_rev_2	21	27	PF00179	0.248
TRG_DiLeu_BaEn_1	31	36	PF01217	0.301
TRG_ENDOCYTIC_2	94	97	PF00928	0.315

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P6Y3	Leptomonas seymouri	45%	100%
A0A0N1HYV4	Leptomonas seymouri	42%	100%
A0A0N1I5S6	Leptomonas seymouri	72%	98%
A0A0N1PE11	Leptomonas seymouri	39%	100%
A0A1X0NNW3	Trypanosomatidae	36%	100%
A0A1X0NW79	Trypanosomatidae	49%	76%
A0A1X0NW95	Trypanosomatidae	45%	100%
A0A1X0NWV3	Trypanosomatidae	48%	100%
A0A1X0NYV3	Trypanosomatidae	40%	100%
A0A3Q8I9V9	Leishmania donovani	38%	100%
A0A3Q8IBP3	Leishmania donovani	40%	100%
A0A3Q8IDP8	Leishmania donovani	83%	100%
A0A3Q8IKN9	Leishmania donovani	44%	100%
A0A3R7MWC6	Trypanosoma rangeli	42%	100%
A0A3S7WW23	Leishmania donovani	38%	100%
A0A3S7WZ86	Leishmania donovani	38%	100%
A0A422MSY8	Trypanosoma rangeli	41%	90%
A0A422MSZ3	Trypanosoma rangeli	43%	100%
A0A422MUY0	Trypanosoma rangeli	51%	100%
A0A422N058	Trypanosoma rangeli	34%	99%
A4H7N9	Leishmania braziliensis	41%	100%
A4H7P0	Leishmania braziliensis	42%	100%
A4HEG7	Leishmania braziliensis	38%	100%
A4HW29	Leishmania infantum	38%	100%
A4HYW5	Leishmania infantum	40%	100%
A4HYX1	Leishmania infantum	83%	100%
A4HYX3	Leishmania infantum	38%	100%
A4HYX4	Leishmania infantum	44%	100%
A4I1M3	Leishmania infantum	38%	100%
E9AIH7	Leishmania braziliensis	36%	100%
E9AII5	Leishmania braziliensis	40%	100%
E9AII6	Leishmania braziliensis	46%	100%
E9APT0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	38%	100%
E9AUR2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	38%	100%
E9AUR8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	79%	100%
E9AUS0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	40%	100%
E9AUS1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	45%	100%
E9AXQ7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	37%	100%
P84150	Leishmania major	30%	100%
Q4Q9R4	Leishmania major	37%	100%
Q4QCS4	Leishmania major	40%	100%
Q4QFP8	Leishmania major	38%	100%
Q5SDH3	Leishmania major	81%	100%
Q5SDH4	Leishmania major	38%	100%
Q5SDH5	Leishmania major	47%	100%