Putative calpain-like cysteine peptidase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

cysteine peptidase, Clan CA, family C2, putative

Species:

Leishmania braziliensis

UniProt:

E9AIH6_LEIBR

TriTrypDb:

LbrM.20.5430 , LBRM2903_200069200

Length:

544

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 1
Forrest at al. (metacyclic)	no	yes: 1
Forrest at al. (procyclic)	no	yes: 2
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 28

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 8

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 12

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 1, no: 71
NetGPI	no	yes: 0, no: 72

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	1
GO:0110165	cellular anatomical entity	1	5
GO:0005737	cytoplasm	2	3
GO:0005930	axoneme	2	1
GO:0005815	microtubule organizing center	2	1
GO:0005856	cytoskeleton	5	1
GO:0020016	ciliary pocket	2	1
GO:0020038	subpellicular network	2	1
GO:0030863	cortical cytoskeleton	6	1
GO:0036064	ciliary basal body	3	1
GO:0043226	organelle	2	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1

Expansion

Sequence features

E9AIH6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AIH6

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	73
GO:0006807	nitrogen compound metabolic process	2	73
GO:0008152	metabolic process	1	73
GO:0019538	protein metabolic process	3	73
GO:0043170	macromolecule metabolic process	3	73
GO:0044238	primary metabolic process	2	73
GO:0071704	organic substance metabolic process	2	73
GO:1901564	organonitrogen compound metabolic process	3	73

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	73
GO:0004175	endopeptidase activity	4	73
GO:0004197	cysteine-type endopeptidase activity	5	73
GO:0004198	calcium-dependent cysteine-type endopeptidase activity	6	73
GO:0008233	peptidase activity	3	73
GO:0008234	cysteine-type peptidase activity	4	73
GO:0016787	hydrolase activity	2	73
GO:0140096	catalytic activity, acting on a protein	2	73

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_MEL_PAP_1	335	341	PF00089	0.464
CLV_NRD_NRD_1	291	293	PF00675	0.342
CLV_NRD_NRD_1	67	69	PF00675	0.281
CLV_PCSK_KEX2_1	291	293	PF00082	0.330
CLV_PCSK_KEX2_1	67	69	PF00082	0.268
CLV_PCSK_SKI1_1	102	106	PF00082	0.289
CLV_PCSK_SKI1_1	325	329	PF00082	0.456
CLV_PCSK_SKI1_1	528	532	PF00082	0.545
DEG_SCF_FBW7_1	417	423	PF00400	0.294
DOC_CKS1_1	417	422	PF01111	0.243
DOC_CYCLIN_RxL_1	197	208	PF00134	0.506
DOC_CYCLIN_yCln2_LP_2	414	420	PF00134	0.233
DOC_MAPK_gen_1	291	298	PF00069	0.267
DOC_MAPK_gen_1	67	77	PF00069	0.465
DOC_MAPK_MEF2A_6	68	77	PF00069	0.480
DOC_PP4_FxxP_1	15	18	PF00568	0.489
DOC_PP4_FxxP_1	314	317	PF00568	0.438
DOC_USP7_MATH_1	121	125	PF00917	0.496
DOC_USP7_MATH_1	168	172	PF00917	0.513
DOC_USP7_MATH_1	182	186	PF00917	0.470
DOC_USP7_UBL2_3	80	84	PF12436	0.481
DOC_WW_Pin1_4	160	165	PF00397	0.509
DOC_WW_Pin1_4	367	372	PF00397	0.438
DOC_WW_Pin1_4	416	421	PF00397	0.338
DOC_WW_Pin1_4	528	533	PF00397	0.450
DOC_WW_Pin1_4	539	544	PF00397	0.562
LIG_14-3-3_CanoR_1	338	342	PF00244	0.443
LIG_14-3-3_CanoR_1	34	38	PF00244	0.470
LIG_14-3-3_CanoR_1	403	407	PF00244	0.523
LIG_14-3-3_CanoR_1	489	495	PF00244	0.437
LIG_Actin_WH2_2	187	205	PF00022	0.494
LIG_APCC_ABBA_1	316	321	PF00400	0.241
LIG_APCC_ABBA_1	461	466	PF00400	0.326
LIG_APCC_ABBAyCdc20_2	315	321	PF00400	0.237
LIG_BIR_II_1	1	5	PF00653	0.182
LIG_BRCT_BRCA1_1	123	127	PF00533	0.535
LIG_BRCT_BRCA1_1	400	404	PF00533	0.451
LIG_BRCT_BRCA1_1	484	488	PF00533	0.477
LIG_BRCT_BRCA1_1	490	494	PF00533	0.481
LIG_deltaCOP1_diTrp_1	299	307	PF00928	0.393
LIG_deltaCOP1_diTrp_1	471	478	PF00928	0.283
LIG_EH1_1	195	203	PF00400	0.519
LIG_FHA_1	143	149	PF00498	0.419
LIG_FHA_1	265	271	PF00498	0.446
LIG_FHA_1	34	40	PF00498	0.497
LIG_FHA_1	6	12	PF00498	0.467
LIG_FHA_2	529	535	PF00498	0.597
LIG_LIR_Apic_2	423	429	PF02991	0.328
LIG_LIR_Apic_2	477	482	PF02991	0.411
LIG_LIR_Gen_1	299	307	PF02991	0.410
LIG_LIR_Gen_1	401	412	PF02991	0.461
LIG_LIR_Gen_1	72	82	PF02991	0.469
LIG_LIR_Nem_3	299	303	PF02991	0.370
LIG_LIR_Nem_3	369	375	PF02991	0.478
LIG_LIR_Nem_3	413	418	PF02991	0.397
LIG_LIR_Nem_3	423	428	PF02991	0.323
LIG_LIR_Nem_3	477	481	PF02991	0.400
LIG_LIR_Nem_3	49	54	PF02991	0.521
LIG_LIR_Nem_3	72	77	PF02991	0.481
LIG_LIR_Nem_3	79	85	PF02991	0.475
LIG_LYPXL_L_2	195	204	PF13949	0.368
LIG_LYPXL_S_1	195	199	PF13949	0.178
LIG_LYPXL_yS_3	196	199	PF13949	0.378
LIG_LYPXL_yS_3	372	375	PF13949	0.417
LIG_PCNA_yPIPBox_3	381	390	PF02747	0.248
LIG_Pex14_1	300	304	PF04695	0.244
LIG_Pex14_1	474	478	PF04695	0.261
LIG_Pex14_2	15	19	PF04695	0.371
LIG_PTAP_UEV_1	276	281	PF05743	0.451
LIG_SH2_CRK	479	483	PF00017	0.518
LIG_SH2_CRK	82	86	PF00017	0.475
LIG_SH2_CRK	89	93	PF00017	0.485
LIG_SH2_GRB2like	520	523	PF00017	0.415
LIG_SH2_GRB2like	89	92	PF00017	0.464
LIG_SH2_PTP2	295	298	PF00017	0.220
LIG_SH2_SRC	112	115	PF00017	0.425
LIG_SH2_SRC	63	66	PF00017	0.386
LIG_SH2_STAP1	89	93	PF00017	0.480
LIG_SH2_STAT5	244	247	PF00017	0.449
LIG_SH2_STAT5	295	298	PF00017	0.393
LIG_SH2_STAT5	306	309	PF00017	0.380
LIG_SH2_STAT5	459	462	PF00017	0.388
LIG_SH2_STAT5	51	54	PF00017	0.494
LIG_SH2_STAT5	520	523	PF00017	0.509
LIG_SH2_STAT5	60	63	PF00017	0.607
LIG_SH3_3	215	221	PF00018	0.465
LIG_SH3_3	274	280	PF00018	0.467
LIG_SH3_3	368	374	PF00018	0.578
LIG_SH3_3	394	400	PF00018	0.461
LIG_SH3_3	414	420	PF00018	0.340
LIG_SH3_3	428	434	PF00018	0.445
LIG_SH3_3	449	455	PF00018	0.697
LIG_SUMO_SIM_par_1	349	356	PF11976	0.387
LIG_SUMO_SIM_par_1	373	378	PF11976	0.412
LIG_TRFH_1	416	420	PF08558	0.428
LIG_TRFH_1	425	429	PF08558	0.377
LIG_TRFH_1	479	483	PF08558	0.261
LIG_WW_3	453	457	PF00397	0.353
MOD_CDK_SPK_2	539	544	PF00069	0.478
MOD_CK1_1	126	132	PF00069	0.478
MOD_CK1_1	151	157	PF00069	0.462
MOD_CK1_1	205	211	PF00069	0.479
MOD_CK1_1	361	367	PF00069	0.629
MOD_CK1_1	495	501	PF00069	0.497
MOD_CK2_1	160	166	PF00069	0.511
MOD_CK2_1	168	174	PF00069	0.465
MOD_CK2_1	349	355	PF00069	0.406
MOD_CK2_1	523	529	PF00069	0.476
MOD_CK2_1	73	79	PF00069	0.468
MOD_CMANNOS	239	242	PF00535	0.331
MOD_CMANNOS	297	300	PF00535	0.355
MOD_GlcNHglycan	174	178	PF01048	0.303
MOD_GlcNHglycan	184	187	PF01048	0.284
MOD_GlcNHglycan	277	280	PF01048	0.452
MOD_GlcNHglycan	307	310	PF01048	0.390
MOD_GlcNHglycan	400	403	PF01048	0.520
MOD_GlcNHglycan	447	450	PF01048	0.774
MOD_GlcNHglycan	484	487	PF01048	0.400
MOD_GSK3_1	122	129	PF00069	0.504
MOD_GSK3_1	151	158	PF00069	0.483
MOD_GSK3_1	271	278	PF00069	0.459
MOD_GSK3_1	349	356	PF00069	0.457
MOD_GSK3_1	363	370	PF00069	0.663
MOD_GSK3_1	398	405	PF00069	0.398
MOD_GSK3_1	406	413	PF00069	0.371
MOD_GSK3_1	416	423	PF00069	0.398
MOD_GSK3_1	488	495	PF00069	0.414
MOD_GSK3_1	516	523	PF00069	0.385
MOD_GSK3_1	87	94	PF00069	0.463
MOD_N-GLC_1	489	494	PF02516	0.508
MOD_N-GLC_2	286	288	PF02516	0.351
MOD_NEK2_1	122	127	PF00069	0.524
MOD_NEK2_1	328	333	PF00069	0.455
MOD_NEK2_1	488	493	PF00069	0.492
MOD_NEK2_1	73	78	PF00069	0.478
MOD_NEK2_2	211	216	PF00069	0.368
MOD_PIKK_1	17	23	PF00454	0.450
MOD_PIKK_1	390	396	PF00454	0.491
MOD_PK_1	271	277	PF00069	0.458
MOD_PKA_2	202	208	PF00069	0.456
MOD_PKA_2	33	39	PF00069	0.465
MOD_PKA_2	337	343	PF00069	0.469
MOD_PKA_2	402	408	PF00069	0.434
MOD_PKA_2	488	494	PF00069	0.461
MOD_PKB_1	231	239	PF00069	0.368
MOD_Plk_1	211	217	PF00069	0.569
MOD_Plk_1	252	258	PF00069	0.409
MOD_Plk_1	261	267	PF00069	0.520
MOD_Plk_1	271	277	PF00069	0.449
MOD_Plk_1	410	416	PF00069	0.414
MOD_Plk_1	489	495	PF00069	0.455
MOD_Plk_1	5	11	PF00069	0.492
MOD_Plk_4	151	157	PF00069	0.506
MOD_Plk_4	168	174	PF00069	0.566
MOD_Plk_4	211	217	PF00069	0.552
MOD_Plk_4	337	343	PF00069	0.428
MOD_Plk_4	410	416	PF00069	0.460
MOD_Plk_4	42	48	PF00069	0.497
MOD_Plk_4	516	522	PF00069	0.442
MOD_Plk_4	73	79	PF00069	0.464
MOD_ProDKin_1	160	166	PF00069	0.509
MOD_ProDKin_1	367	373	PF00069	0.423
MOD_ProDKin_1	416	422	PF00069	0.336
MOD_ProDKin_1	528	534	PF00069	0.450
TRG_DiLeu_BaEn_1	6	11	PF01217	0.398
TRG_DiLeu_BaLyEn_6	127	132	PF01217	0.490
TRG_ENDOCYTIC_2	196	199	PF00928	0.503
TRG_ENDOCYTIC_2	295	298	PF00928	0.220
TRG_ENDOCYTIC_2	304	307	PF00928	0.246
TRG_ENDOCYTIC_2	372	375	PF00928	0.417
TRG_ENDOCYTIC_2	425	428	PF00928	0.338
TRG_ENDOCYTIC_2	51	54	PF00928	0.525
TRG_ENDOCYTIC_2	82	85	PF00928	0.482
TRG_ENDOCYTIC_2	89	92	PF00928	0.490
TRG_Pf-PMV_PEXEL_1	130	135	PF00026	0.189
TRG_Pf-PMV_PEXEL_1	430	435	PF00026	0.481

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8T6	Leptomonas seymouri	28%	78%
A0A0N1IGQ2	Leptomonas seymouri	26%	77%
A0A0N1ILF1	Leptomonas seymouri	32%	79%
A0A0N1IMH1	Leptomonas seymouri	29%	69%
A0A0N1PCA9	Leptomonas seymouri	30%	74%
A0A0N1PFI4	Leptomonas seymouri	29%	70%
A0A0S4JLK6	Bodo saltans	24%	67%
A0A0S4JS70	Bodo saltans	23%	71%
A0A0S4KGT2	Bodo saltans	31%	72%
A0A1X0NJK2	Trypanosomatidae	28%	72%
A0A1X0NJX8	Trypanosomatidae	27%	70%
A0A1X0NKX8	Trypanosomatidae	28%	67%
A0A1X0NW84	Trypanosomatidae	31%	79%
A0A1X0NW85	Trypanosomatidae	31%	80%
A0A1X0NWW1	Trypanosomatidae	34%	75%
A0A3Q8IDD4	Leishmania donovani	24%	77%
A0A3S5ISG2	Trypanosoma rangeli	31%	70%
A0A3S7WW18	Leishmania donovani	28%	66%
A0A3S7WW41	Leishmania donovani	29%	73%
A0A3S7WW71	Leishmania donovani	31%	79%
A0A3S7X430	Leishmania donovani	29%	69%
A0A3S7X460	Leishmania donovani	29%	70%
A0A3S7X470	Leishmania donovani	28%	75%
A0A422MYX0	Trypanosoma rangeli	34%	75%
A4HE81	Leishmania braziliensis	24%	100%
A4HJ14	Leishmania braziliensis	29%	69%
A4HJ23	Leishmania braziliensis	30%	96%
A4HJ24	Leishmania braziliensis	29%	100%
A4HYN0	Leishmania infantum	28%	66%
A4HYW2	Leishmania infantum	31%	79%
A4HYW3	Leishmania infantum	29%	73%
A4I1J4	Leishmania infantum	24%	77%
A4I6E4	Leishmania infantum	29%	75%
A4I6E6	Leishmania infantum	29%	70%
A4I6K4	Leishmania infantum	29%	69%
C9ZIE8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	32%	80%
C9ZIE9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	34%	74%
C9ZN52	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	29%	69%
C9ZWY4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	27%	68%
E8NHF1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	100%
E8NHG6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	75%
E8NHG8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	69%
E8NHM2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	77%
E8NHM4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	77%
E8NHM8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	94%
E8NHQ2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	100%
E9AIH1	Leishmania braziliensis	27%	67%
E9AIH3	Leishmania braziliensis	31%	100%
E9AIH4	Leishmania braziliensis	29%	100%
E9AK26	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	68%
E9AUQ8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	27%	84%
E9AUQ9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	79%
E9AUR0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	73%
E9AUR1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	76%	99%
E9AXM9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	77%
E9B1J0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	69%
E9B1J6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	77%
Q4Q6L7	Leishmania major	28%	100%
Q4Q6L9	Leishmania major	29%	100%
Q4Q6M2	Leishmania major	22%	72%
Q4Q9U3	Leishmania major	24%	100%
Q4QCS5	Leishmania major	73%	100%
Q4QCS6	Leishmania major	29%	100%
Q4QCS7	Leishmania major	31%	100%
Q4QCS8	Leishmania major	28%	66%
V5AYJ1	Trypanosoma cruzi	29%	76%
V5BA05	Trypanosoma cruzi	34%	82%
V5BEL3	Trypanosoma cruzi	33%	79%
V5BN20	Trypanosoma cruzi	30%	70%
V5D5V8	Trypanosoma cruzi	30%	73%
V5DES7	Trypanosoma cruzi	29%	69%