Putative calpain-like cysteine peptidase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

cysteine peptidase, Clan CA, family C2, putative

Species:

Leishmania braziliensis

UniProt:

E9AIH3_LEIBR

TriTrypDb:

LbrM.20.5400 , LBRM2903_200068900

Length:

687

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 1
Forrest at al. (metacyclic)	no	yes: 3
Forrest at al. (procyclic)	no	yes: 4
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 36

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 16

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 20

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 2, no: 92
NetGPI	no	yes: 0, no: 94

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	1
GO:0110165	cellular anatomical entity	1	5
GO:0005737	cytoplasm	2	3
GO:0005930	axoneme	2	1
GO:0005815	microtubule organizing center	2	1
GO:0005856	cytoskeleton	5	1
GO:0020016	ciliary pocket	2	1
GO:0020038	subpellicular network	2	1
GO:0030863	cortical cytoskeleton	6	1
GO:0036064	ciliary basal body	3	1
GO:0043226	organelle	2	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1

Expansion

Sequence features

E9AIH3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AIH3

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	95
GO:0006807	nitrogen compound metabolic process	2	95
GO:0008152	metabolic process	1	95
GO:0019538	protein metabolic process	3	95
GO:0043170	macromolecule metabolic process	3	95
GO:0044238	primary metabolic process	2	95
GO:0071704	organic substance metabolic process	2	95
GO:1901564	organonitrogen compound metabolic process	3	95

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	95
GO:0004175	endopeptidase activity	4	95
GO:0004197	cysteine-type endopeptidase activity	5	95
GO:0004198	calcium-dependent cysteine-type endopeptidase activity	6	95
GO:0008233	peptidase activity	3	95
GO:0008234	cysteine-type peptidase activity	4	95
GO:0016787	hydrolase activity	2	95
GO:0140096	catalytic activity, acting on a protein	2	95

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	230	234	PF00656	0.435
CLV_NRD_NRD_1	524	526	PF00675	0.498
CLV_PCSK_KEX2_1	453	455	PF00082	0.266
CLV_PCSK_KEX2_1	524	526	PF00082	0.294
CLV_PCSK_KEX2_1	538	540	PF00082	0.434
CLV_PCSK_PC1ET2_1	453	455	PF00082	0.265
CLV_PCSK_PC1ET2_1	538	540	PF00082	0.440
CLV_PCSK_SKI1_1	156	160	PF00082	0.477
CLV_PCSK_SKI1_1	251	255	PF00082	0.247
CLV_PCSK_SKI1_1	634	638	PF00082	0.397
CLV_PCSK_SKI1_1	670	674	PF00082	0.459
DEG_Nend_Nbox_1	1	3	PF02207	0.695
DOC_CKS1_1	380	385	PF01111	0.456
DOC_CYCLIN_yCln2_LP_2	620	626	PF00134	0.397
DOC_MAPK_FxFP_2	177	180	PF00069	0.464
DOC_MAPK_gen_1	524	531	PF00069	0.384
DOC_MAPK_HePTP_8	521	533	PF00069	0.255
DOC_MAPK_MEF2A_6	524	533	PF00069	0.353
DOC_MAPK_MEF2A_6	628	637	PF00069	0.399
DOC_MAPK_MEF2A_6	670	678	PF00069	0.490
DOC_PP1_RVXF_1	169	175	PF00149	0.392
DOC_PP1_RVXF_1	632	638	PF00149	0.359
DOC_PP1_RVXF_1	67	73	PF00149	0.198
DOC_PP2B_LxvP_1	602	605	PF13499	0.330
DOC_PP2B_PxIxI_1	673	679	PF00149	0.473
DOC_PP4_FxxP_1	177	180	PF00568	0.501
DOC_PP4_FxxP_1	254	257	PF00568	0.457
DOC_PP4_FxxP_1	380	383	PF00568	0.464
DOC_USP7_MATH_1	345	349	PF00917	0.449
DOC_USP7_MATH_2	180	186	PF00917	0.469
DOC_USP7_UBL2_3	317	321	PF12436	0.454
DOC_WW_Pin1_4	35	40	PF00397	0.324
DOC_WW_Pin1_4	379	384	PF00397	0.465
DOC_WW_Pin1_4	496	501	PF00397	0.457
DOC_WW_Pin1_4	574	579	PF00397	0.424
DOC_WW_Pin1_4	592	597	PF00397	0.302
LIG_14-3-3_CanoR_1	273	277	PF00244	0.440
LIG_14-3-3_CanoR_1	437	447	PF00244	0.467
LIG_14-3-3_CanoR_1	56	60	PF00244	0.273
LIG_14-3-3_CanoR_1	606	615	PF00244	0.359
LIG_14-3-3_CanoR_1	97	103	PF00244	0.355
LIG_AP2alpha_1	174	178	PF02296	0.338
LIG_APCC_ABBA_1	588	593	PF00400	0.392
LIG_BIR_III_2	36	40	PF00653	0.359
LIG_deltaCOP1_diTrp_1	446	451	PF00928	0.443
LIG_deltaCOP1_diTrp_1	481	489	PF00928	0.462
LIG_eIF4E_1	407	413	PF01652	0.483
LIG_FHA_1	117	123	PF00498	0.324
LIG_FHA_1	145	151	PF00498	0.414
LIG_FHA_1	281	287	PF00498	0.458
LIG_FHA_1	359	365	PF00498	0.449
LIG_FHA_1	415	421	PF00498	0.461
LIG_FHA_1	615	621	PF00498	0.319
LIG_FHA_1	628	634	PF00498	0.494
LIG_FHA_1	97	103	PF00498	0.315
LIG_FHA_2	157	163	PF00498	0.378
LIG_FHA_2	355	361	PF00498	0.452
LIG_FHA_2	399	405	PF00498	0.452
LIG_FHA_2	441	447	PF00498	0.411
LIG_FHA_2	515	521	PF00498	0.377
LIG_FHA_2	658	664	PF00498	0.433
LIG_FHA_2	84	90	PF00498	0.232
LIG_LIR_Apic_2	176	181	PF02991	0.443
LIG_LIR_Apic_2	25	31	PF02991	0.473
LIG_LIR_Apic_2	252	257	PF02991	0.459
LIG_LIR_Apic_2	445	450	PF02991	0.455
LIG_LIR_Gen_1	139	148	PF02991	0.362
LIG_LIR_Gen_1	149	158	PF02991	0.326
LIG_LIR_Gen_1	316	326	PF02991	0.461
LIG_LIR_Gen_1	360	368	PF02991	0.466
LIG_LIR_Gen_1	417	426	PF02991	0.490
LIG_LIR_Gen_1	509	519	PF02991	0.364
LIG_LIR_Gen_1	577	588	PF02991	0.409
LIG_LIR_Gen_1	599	608	PF02991	0.311
LIG_LIR_Gen_1	609	618	PF02991	0.322
LIG_LIR_Gen_1	648	658	PF02991	0.461
LIG_LIR_LC3C_4	599	604	PF02991	0.384
LIG_LIR_Nem_3	139	143	PF02991	0.359
LIG_LIR_Nem_3	149	154	PF02991	0.303
LIG_LIR_Nem_3	288	293	PF02991	0.480
LIG_LIR_Nem_3	309	314	PF02991	0.447
LIG_LIR_Nem_3	316	322	PF02991	0.456
LIG_LIR_Nem_3	360	365	PF02991	0.446
LIG_LIR_Nem_3	417	421	PF02991	0.488
LIG_LIR_Nem_3	455	461	PF02991	0.425
LIG_LIR_Nem_3	466	470	PF02991	0.428
LIG_LIR_Nem_3	509	514	PF02991	0.346
LIG_LIR_Nem_3	577	583	PF02991	0.430
LIG_LIR_Nem_3	589	594	PF02991	0.357
LIG_LIR_Nem_3	599	604	PF02991	0.302
LIG_LIR_Nem_3	609	614	PF02991	0.298
LIG_LIR_Nem_3	648	653	PF02991	0.445
LIG_LYPXL_SIV_4	406	414	PF13949	0.433
LIG_Pex14_2	174	178	PF04695	0.450
LIG_Pex14_2	349	353	PF04695	0.442
LIG_Pex14_2	478	482	PF04695	0.455
LIG_PTB_Apo_2	370	377	PF02174	0.392
LIG_PTB_Apo_2	90	97	PF02174	0.164
LIG_PTB_Phospho_1	370	376	PF10480	0.392
LIG_PTB_Phospho_1	90	96	PF10480	0.164
LIG_SH2_CRK	319	323	PF00017	0.453
LIG_SH2_CRK	326	330	PF00017	0.455
LIG_SH2_CRK	371	375	PF00017	0.486
LIG_SH2_CRK	418	422	PF00017	0.469
LIG_SH2_CRK	580	584	PF00017	0.436
LIG_SH2_GRB2like	371	374	PF00017	0.484
LIG_SH2_NCK_1	151	155	PF00017	0.517
LIG_SH2_NCK_1	319	323	PF00017	0.472
LIG_SH2_NCK_1	407	411	PF00017	0.472
LIG_SH2_NCK_1	461	465	PF00017	0.489
LIG_SH2_SRC	290	293	PF00017	0.460
LIG_SH2_SRC	376	379	PF00017	0.448
LIG_SH2_SRC	624	627	PF00017	0.413
LIG_SH2_STAP1	319	323	PF00017	0.473
LIG_SH2_STAP1	429	433	PF00017	0.380
LIG_SH2_STAP1	461	465	PF00017	0.500
LIG_SH2_STAP1	502	506	PF00017	0.312
LIG_SH2_STAT5	131	134	PF00017	0.391
LIG_SH2_STAT5	290	293	PF00017	0.464
LIG_SH2_STAT5	425	428	PF00017	0.448
LIG_SH2_STAT5	461	464	PF00017	0.500
LIG_SH2_STAT5	467	470	PF00017	0.491
LIG_SH2_STAT5	488	491	PF00017	0.448
LIG_SH2_STAT5	601	604	PF00017	0.318
LIG_SH2_STAT5	677	680	PF00017	0.463
LIG_SH3_3	520	526	PF00018	0.356
LIG_SH3_5	500	504	PF00018	0.389
LIG_SUMO_SIM_par_1	547	552	PF11976	0.382
LIG_TYR_ITSM	147	154	PF00017	0.529
LIG_UBA3_1	191	195	PF00899	0.451
LIG_UBA3_1	364	369	PF00899	0.472
LIG_UBA3_1	45	52	PF00899	0.318
MOD_CDK_SPxK_1	379	385	PF00069	0.475
MOD_CK1_1	234	240	PF00069	0.468
MOD_CK1_1	396	402	PF00069	0.482
MOD_CK2_1	310	316	PF00069	0.443
MOD_CK2_1	354	360	PF00069	0.466
MOD_CK2_1	398	404	PF00069	0.457
MOD_CK2_1	514	520	PF00069	0.358
MOD_CK2_1	657	663	PF00069	0.439
MOD_CK2_1	83	89	PF00069	0.278
MOD_GlcNHglycan	340	344	PF01048	0.270
MOD_GlcNHglycan	4	7	PF01048	0.624
MOD_GlcNHglycan	471	474	PF01048	0.288
MOD_GlcNHglycan	568	571	PF01048	0.405
MOD_GlcNHglycan	77	80	PF01048	0.251
MOD_GSK3_1	112	119	PF00069	0.261
MOD_GSK3_1	354	361	PF00069	0.485
MOD_GSK3_1	393	400	PF00069	0.488
MOD_GSK3_1	438	445	PF00069	0.454
MOD_GSK3_1	582	589	PF00069	0.345
MOD_GSK3_1	592	599	PF00069	0.364
MOD_GSK3_1	677	684	PF00069	0.332
MOD_GSK3_1	92	99	PF00069	0.318
MOD_LATS_1	367	373	PF00433	0.355
MOD_N-GLC_1	358	363	PF02516	0.255
MOD_N-GLC_1	369	374	PF02516	0.319
MOD_N-GLC_1	60	65	PF02516	0.297
MOD_N-GLC_1	92	97	PF02516	0.418
MOD_NEK2_1	163	168	PF00069	0.340
MOD_NEK2_1	231	236	PF00069	0.470
MOD_NEK2_1	300	305	PF00069	0.460
MOD_NEK2_1	353	358	PF00069	0.506
MOD_NEK2_1	414	419	PF00069	0.471
MOD_NEK2_1	519	524	PF00069	0.356
MOD_NEK2_1	83	88	PF00069	0.261
MOD_NEK2_2	98	103	PF00069	0.291
MOD_PIKK_1	398	404	PF00454	0.338
MOD_PIKK_1	438	444	PF00454	0.504
MOD_PIKK_1	531	537	PF00454	0.364
MOD_PIKK_1	606	612	PF00454	0.425
MOD_PIKK_1	63	69	PF00454	0.344
MOD_PKA_1	459	465	PF00069	0.496
MOD_PKA_1	538	544	PF00069	0.416
MOD_PKA_2	272	278	PF00069	0.435
MOD_PKA_2	506	512	PF00069	0.367
MOD_PKA_2	538	544	PF00069	0.415
MOD_PKA_2	55	61	PF00069	0.315
MOD_PKA_2	96	102	PF00069	0.335
MOD_Plk_1	354	360	PF00069	0.500
MOD_Plk_1	519	525	PF00069	0.323
MOD_Plk_1	586	592	PF00069	0.360
MOD_Plk_4	12	18	PF00069	0.550
MOD_Plk_4	146	152	PF00069	0.381
MOD_Plk_4	187	193	PF00069	0.452
MOD_Plk_4	231	237	PF00069	0.467
MOD_Plk_4	281	287	PF00069	0.484
MOD_Plk_4	310	316	PF00069	0.429
MOD_Plk_4	416	422	PF00069	0.470
MOD_Plk_4	474	480	PF00069	0.457
MOD_Plk_4	506	512	PF00069	0.406
MOD_Plk_4	586	592	PF00069	0.395
MOD_Plk_4	677	683	PF00069	0.383
MOD_Plk_4	98	104	PF00069	0.320
MOD_ProDKin_1	35	41	PF00069	0.324
MOD_ProDKin_1	379	385	PF00069	0.465
MOD_ProDKin_1	496	502	PF00069	0.456
MOD_ProDKin_1	574	580	PF00069	0.421
MOD_ProDKin_1	592	598	PF00069	0.301
MOD_SUMO_for_1	143	146	PF00179	0.393
MOD_SUMO_rev_2	176	186	PF00179	0.452
TRG_DiLeu_BaEn_1	408	413	PF01217	0.512
TRG_DiLeu_BaEn_2	105	111	PF01217	0.243
TRG_DiLeu_BaLyEn_6	429	434	PF01217	0.517
TRG_ENDOCYTIC_2	140	143	PF00928	0.375
TRG_ENDOCYTIC_2	151	154	PF00928	0.354
TRG_ENDOCYTIC_2	290	293	PF00928	0.485
TRG_ENDOCYTIC_2	319	322	PF00928	0.455
TRG_ENDOCYTIC_2	326	329	PF00928	0.453
TRG_ENDOCYTIC_2	362	365	PF00928	0.494
TRG_ENDOCYTIC_2	371	374	PF00928	0.463
TRG_ENDOCYTIC_2	418	421	PF00928	0.476
TRG_ENDOCYTIC_2	461	464	PF00928	0.418
TRG_ENDOCYTIC_2	467	470	PF00928	0.418
TRG_ENDOCYTIC_2	580	583	PF00928	0.449
TRG_ENDOCYTIC_2	601	604	PF00928	0.317
TRG_ER_diArg_1	504	507	PF00400	0.282
TRG_ER_diArg_1	523	525	PF00400	0.202
TRG_NES_CRM1_1	187	200	PF08389	0.489
TRG_Pf-PMV_PEXEL_1	638	643	PF00026	0.371

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8T6	Leptomonas seymouri	35%	98%
A0A0N1I8N2	Leptomonas seymouri	28%	70%
A0A0N1IGQ2	Leptomonas seymouri	32%	97%
A0A0N1ILF1	Leptomonas seymouri	87%	100%
A0A0N1IMH1	Leptomonas seymouri	34%	88%
A0A0N1P9P1	Leptomonas seymouri	36%	81%
A0A0N1PCA9	Leptomonas seymouri	36%	93%
A0A0N1PE91	Leptomonas seymouri	24%	77%
A0A0N1PFI4	Leptomonas seymouri	33%	89%
A0A0S4JLK6	Bodo saltans	29%	85%
A0A0S4JS70	Bodo saltans	25%	89%
A0A0S4KGT2	Bodo saltans	36%	91%
A0A0S4KKP7	Bodo saltans	26%	92%
A0A1X0NJ61	Trypanosomatidae	34%	100%
A0A1X0NJK2	Trypanosomatidae	33%	91%
A0A1X0NJX8	Trypanosomatidae	36%	88%
A0A1X0NKT7	Trypanosomatidae	31%	86%
A0A1X0NKX8	Trypanosomatidae	33%	84%
A0A1X0NMT3	Trypanosomatidae	39%	81%
A0A1X0NW84	Trypanosomatidae	60%	99%
A0A1X0NW85	Trypanosomatidae	41%	100%
A0A1X0NW89	Trypanosomatidae	43%	80%
A0A1X0NWA6	Trypanosomatidae	27%	79%
A0A1X0NWW1	Trypanosomatidae	42%	95%
A0A3Q8IBS3	Leishmania donovani	40%	75%
A0A3Q8IDD4	Leishmania donovani	32%	97%
A0A3S5H5A5	Leishmania donovani	37%	80%
A0A3S5ISG2	Trypanosoma rangeli	33%	89%
A0A3S7WW18	Leishmania donovani	40%	84%
A0A3S7WW41	Leishmania donovani	33%	92%
A0A3S7WW71	Leishmania donovani	91%	100%
A0A3S7X430	Leishmania donovani	34%	88%
A0A3S7X438	Leishmania donovani	30%	73%
A0A3S7X460	Leishmania donovani	34%	88%
A0A3S7X463	Leishmania donovani	26%	73%
A0A3S7X470	Leishmania donovani	32%	95%
A0A422MYU1	Trypanosoma rangeli	40%	75%
A0A422MYX0	Trypanosoma rangeli	41%	94%
A4H3W4	Leishmania braziliensis	34%	100%
A4HE81	Leishmania braziliensis	33%	100%
A4HFH7	Leishmania braziliensis	22%	100%
A4HJ14	Leishmania braziliensis	35%	100%
A4HJ21	Leishmania braziliensis	25%	100%
A4HJ22	Leishmania braziliensis	30%	100%
A4HJ23	Leishmania braziliensis	32%	97%
A4HJ24	Leishmania braziliensis	34%	100%
A4HS39	Leishmania infantum	37%	80%
A4HYN0	Leishmania infantum	40%	84%
A4HYW1	Leishmania infantum	40%	68%
A4HYW2	Leishmania infantum	91%	100%
A4HYW3	Leishmania infantum	33%	92%
A4HYW4	Leishmania infantum	28%	69%
A4I1J4	Leishmania infantum	33%	97%
A4I6E4	Leishmania infantum	32%	95%
A4I6E6	Leishmania infantum	34%	88%
A4I6F0	Leishmania infantum	30%	73%
A4I6K4	Leishmania infantum	34%	88%
A4I6K5	Leishmania infantum	26%	73%
A4I6K6	Leishmania infantum	23%	91%
A8MX76	Homo sapiens	25%	100%
C9ZIE8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	52%	100%
C9ZIE9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	38%	94%
C9ZN52	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	34%	87%
C9ZN53	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	29%	81%
C9ZWY4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	29%	85%
C9ZY36	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	35%	80%
E8NHF1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	37%	100%
E8NHG6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	32%	95%
E8NHG7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	30%	73%
E8NHG8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	87%
E8NHM2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	33%	97%
E8NHM4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	97%
E8NHM8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
E9AIH1	Leishmania braziliensis	40%	100%
E9AIH4	Leishmania braziliensis	34%	92%
E9AIH6	Leishmania braziliensis	31%	100%
E9AK26	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	86%
E9AUQ7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	40%	74%
E9AUQ9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
E9AUR0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	33%	92%
E9AUR1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	30%	100%
E9AXM9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	33%	97%
E9B1J0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	88%
E9B1J1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	73%
E9B1J2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	23%	91%
E9B1J6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	97%
O08688	Mus musculus	26%	100%
O15484	Homo sapiens	26%	100%
Q4Q6L7	Leishmania major	34%	100%
Q4Q6L9	Leishmania major	31%	100%
Q4Q6M0	Leishmania major	31%	100%
Q4Q6M2	Leishmania major	23%	100%
Q4Q6M3	Leishmania major	26%	100%
Q4Q6M4	Leishmania major	35%	100%
Q4Q9U3	Leishmania major	33%	100%
Q4QCS6	Leishmania major	33%	100%
Q4QCS7	Leishmania major	91%	100%
Q4QCS8	Leishmania major	39%	100%
Q4QCS9	Leishmania major	39%	100%
Q8R4C0	Rattus norvegicus	26%	100%
Q9U0T9	Leishmania major	35%	100%
V5AYJ1	Trypanosoma cruzi	32%	96%
V5B5I4	Trypanosoma cruzi	41%	82%
V5BA05	Trypanosoma cruzi	42%	100%
V5BEL3	Trypanosoma cruzi	61%	99%
V5BN20	Trypanosoma cruzi	36%	89%
V5D5V8	Trypanosoma cruzi	29%	93%
V5D9Y2	Trypanosoma cruzi	36%	81%
V5DES7	Trypanosoma cruzi	32%	87%