Putative calpain-like cysteine peptidase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

cysteine peptidase, Clan CA, family C2, putative

Species:

Leishmania braziliensis

UniProt:

E9AIH1_LEIBR

TriTrypDb:

LbrM.20.5380 , LBRM2903_200068500 , LBRM2903_200068600

Length:

816

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 1
Forrest at al. (metacyclic)	no	yes: 3
Forrest at al. (procyclic)	no	yes: 4
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 36

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 16

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 20

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 2, no: 102
NetGPI	no	yes: 0, no: 104

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	3
GO:0110165	cellular anatomical entity	1	5
GO:0005815	microtubule organizing center	2	1
GO:0005856	cytoskeleton	5	1
GO:0020016	ciliary pocket	2	1
GO:0020038	subpellicular network	2	1
GO:0030863	cortical cytoskeleton	6	1
GO:0036064	ciliary basal body	3	1
GO:0043226	organelle	2	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0005930	axoneme	2	1
GO:0016020	membrane	2	1

Expansion

Sequence features

E9AIH1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AIH1

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	105
GO:0006807	nitrogen compound metabolic process	2	105
GO:0008152	metabolic process	1	105
GO:0019538	protein metabolic process	3	105
GO:0043170	macromolecule metabolic process	3	105
GO:0044238	primary metabolic process	2	105
GO:0071704	organic substance metabolic process	2	105
GO:1901564	organonitrogen compound metabolic process	3	105

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	105
GO:0004175	endopeptidase activity	4	105
GO:0004197	cysteine-type endopeptidase activity	5	105
GO:0004198	calcium-dependent cysteine-type endopeptidase activity	6	105
GO:0008233	peptidase activity	3	105
GO:0008234	cysteine-type peptidase activity	4	105
GO:0016787	hydrolase activity	2	105
GO:0140096	catalytic activity, acting on a protein	2	105

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	300	304	PF00656	0.550
CLV_C14_Caspase3-7	375	379	PF00656	0.504
CLV_NRD_NRD_1	281	283	PF00675	0.536
CLV_NRD_NRD_1	669	671	PF00675	0.460
CLV_PCSK_KEX2_1	281	283	PF00082	0.503
CLV_PCSK_KEX2_1	669	671	PF00082	0.464
CLV_PCSK_KEX2_1	769	771	PF00082	0.535
CLV_PCSK_KEX2_1	799	801	PF00082	0.536
CLV_PCSK_PC1ET2_1	769	771	PF00082	0.547
CLV_PCSK_PC1ET2_1	799	801	PF00082	0.580
CLV_PCSK_SKI1_1	124	128	PF00082	0.665
CLV_PCSK_SKI1_1	282	286	PF00082	0.634
CLV_PCSK_SKI1_1	337	341	PF00082	0.314
CLV_PCSK_SKI1_1	428	432	PF00082	0.377
CLV_PCSK_SKI1_1	444	448	PF00082	0.234
DEG_MDM2_SWIB_1	609	616	PF02201	0.523
DEG_SIAH_1	114	122	PF03145	0.451
DOC_ANK_TNKS_1	799	806	PF00023	0.339
DOC_CYCLIN_yCln2_LP_2	740	746	PF00134	0.655
DOC_PP1_RVXF_1	338	345	PF00149	0.434
DOC_PP1_RVXF_1	416	422	PF00149	0.533
DOC_PP1_RVXF_1	573	580	PF00149	0.592
DOC_PP4_FxxP_1	186	189	PF00568	0.351
DOC_PP4_FxxP_1	21	24	PF00568	0.528
DOC_PP4_FxxP_1	319	322	PF00568	0.576
DOC_PP4_FxxP_1	399	402	PF00568	0.537
DOC_USP7_MATH_1	257	261	PF00917	0.471
DOC_USP7_MATH_1	299	303	PF00917	0.574
DOC_USP7_MATH_1	386	390	PF00917	0.534
DOC_USP7_MATH_1	54	58	PF00917	0.680
DOC_USP7_MATH_1	64	68	PF00917	0.651
DOC_USP7_MATH_2	352	358	PF00917	0.588
DOC_USP7_UBL2_3	462	466	PF12436	0.532
DOC_USP7_UBL2_3	614	618	PF12436	0.560
DOC_WW_Pin1_4	36	41	PF00397	0.647
DOC_WW_Pin1_4	521	526	PF00397	0.538
DOC_WW_Pin1_4	653	658	PF00397	0.443
LIG_14-3-3_CanoR_1	124	130	PF00244	0.680
LIG_14-3-3_CanoR_1	281	289	PF00244	0.595
LIG_14-3-3_CanoR_1	418	422	PF00244	0.518
LIG_14-3-3_CanoR_1	706	715	PF00244	0.597
LIG_Actin_WH2_2	429	446	PF00022	0.615
LIG_BIR_II_1	1	5	PF00653	0.560
LIG_BRCT_BRCA1_1	764	768	PF00533	0.521
LIG_Clathr_ClatBox_1	788	792	PF01394	0.618
LIG_CtBP_PxDLS_1	352	356	PF00389	0.437
LIG_deltaCOP1_diTrp_1	710	714	PF00928	0.487
LIG_eIF4E_1	540	546	PF01652	0.551
LIG_FHA_1	121	127	PF00498	0.690
LIG_FHA_1	263	269	PF00498	0.390
LIG_FHA_1	640	646	PF00498	0.472
LIG_FHA_1	659	665	PF00498	0.561
LIG_FHA_2	249	255	PF00498	0.468
LIG_FHA_2	298	304	PF00498	0.549
LIG_FHA_2	550	556	PF00498	0.545
LIG_FHA_2	586	592	PF00498	0.612
LIG_LIR_Apic_2	184	189	PF02991	0.346
LIG_LIR_Apic_2	19	24	PF02991	0.529
LIG_LIR_Apic_2	90	96	PF02991	0.524
LIG_LIR_Gen_1	15	24	PF02991	0.528
LIG_LIR_Gen_1	461	472	PF02991	0.538
LIG_LIR_Gen_1	487	494	PF02991	0.566
LIG_LIR_Gen_1	709	720	PF02991	0.487
LIG_LIR_Nem_3	15	21	PF02991	0.531
LIG_LIR_Nem_3	202	206	PF02991	0.357
LIG_LIR_Nem_3	219	223	PF02991	0.433
LIG_LIR_Nem_3	433	438	PF02991	0.560
LIG_LIR_Nem_3	454	459	PF02991	0.521
LIG_LIR_Nem_3	461	467	PF02991	0.528
LIG_LIR_Nem_3	470	474	PF02991	0.520
LIG_LIR_Nem_3	612	616	PF02991	0.527
LIG_LIR_Nem_3	656	662	PF02991	0.368
LIG_LIR_Nem_3	676	681	PF02991	0.487
LIG_LIR_Nem_3	692	697	PF02991	0.511
LIG_LIR_Nem_3	709	715	PF02991	0.509
LIG_LIR_Nem_3	765	771	PF02991	0.434
LIG_LIR_Nem_3	779	784	PF02991	0.514
LIG_PDZ_Class_3	811	816	PF00595	0.544
LIG_Pex14_2	609	613	PF04695	0.530
LIG_SH2_CRK	223	227	PF00017	0.369
LIG_SH2_CRK	464	468	PF00017	0.527
LIG_SH2_CRK	471	475	PF00017	0.527
LIG_SH2_CRK	518	522	PF00017	0.547
LIG_SH2_CRK	678	682	PF00017	0.456
LIG_SH2_CRK	694	698	PF00017	0.533
LIG_SH2_CRK	93	97	PF00017	0.560
LIG_SH2_GRB2like	277	280	PF00017	0.601
LIG_SH2_GRB2like	619	622	PF00017	0.496
LIG_SH2_NCK_1	100	104	PF00017	0.531
LIG_SH2_NCK_1	540	544	PF00017	0.547
LIG_SH2_NCK_1	93	97	PF00017	0.553
LIG_SH2_PTP2	731	734	PF00017	0.436
LIG_SH2_SRC	100	103	PF00017	0.516
LIG_SH2_SRC	107	110	PF00017	0.495
LIG_SH2_SRC	435	438	PF00017	0.546
LIG_SH2_SRC	558	561	PF00017	0.565
LIG_SH2_STAP1	100	104	PF00017	0.545
LIG_SH2_STAP1	212	216	PF00017	0.371
LIG_SH2_STAP1	445	449	PF00017	0.563
LIG_SH2_STAP1	633	637	PF00017	0.404
LIG_SH2_STAP1	77	81	PF00017	0.824
LIG_SH2_STAT5	107	110	PF00017	0.498
LIG_SH2_STAT5	242	245	PF00017	0.417
LIG_SH2_STAT5	435	438	PF00017	0.537
LIG_SH2_STAT5	518	521	PF00017	0.554
LIG_SH2_STAT5	551	554	PF00017	0.510
LIG_SH2_STAT5	558	561	PF00017	0.522
LIG_SH2_STAT5	619	622	PF00017	0.526
LIG_SH2_STAT5	62	65	PF00017	0.809
LIG_SH2_STAT5	731	734	PF00017	0.380
LIG_SH2_STAT5	749	752	PF00017	0.404
LIG_SH2_STAT5	85	88	PF00017	0.830
LIG_SH3_3	244	250	PF00018	0.375
LIG_SH3_3	319	325	PF00018	0.575
LIG_SH3_3	335	341	PF00018	0.603
LIG_SH3_3	346	352	PF00018	0.454
LIG_SH3_3	651	657	PF00018	0.427
LIG_SH3_3	740	746	PF00018	0.639
LIG_SH3_3	788	794	PF00018	0.512
LIG_SH3_5	631	635	PF00018	0.481
LIG_SUMO_SIM_anti_2	719	724	PF11976	0.428
LIG_SUMO_SIM_anti_2	763	768	PF11976	0.510
LIG_SUMO_SIM_par_1	647	653	PF11976	0.444
LIG_SUMO_SIM_par_1	679	684	PF11976	0.435
LIG_TRAF2_1	41	44	PF00917	0.540
LIG_TRAF2_1	76	79	PF00917	0.838
LIG_TRAF2_1	99	102	PF00917	0.515
LIG_TYR_ITIM	191	196	PF00017	0.388
LIG_TYR_ITIM	516	521	PF00017	0.598
LIG_UBA3_1	191	200	PF00899	0.405
LIG_UBA3_1	722	730	PF00899	0.496
LIG_WW_1	59	62	PF00397	0.855
MOD_CDK_SPxK_1	521	527	PF00069	0.544
MOD_CK1_1	34	40	PF00069	0.736
MOD_CK1_1	384	390	PF00069	0.553
MOD_CK2_1	140	146	PF00069	0.571
MOD_CK2_1	248	254	PF00069	0.542
MOD_CK2_1	384	390	PF00069	0.523
MOD_CK2_1	549	555	PF00069	0.592
MOD_CK2_1	681	687	PF00069	0.426
MOD_CMANNOS	610	613	PF00535	0.331
MOD_GlcNHglycan	115	118	PF01048	0.621
MOD_GlcNHglycan	367	370	PF01048	0.356
MOD_GlcNHglycan	438	441	PF01048	0.360
MOD_GlcNHglycan	447	450	PF01048	0.366
MOD_GlcNHglycan	475	478	PF01048	0.326
MOD_GlcNHglycan	579	582	PF01048	0.384
MOD_GlcNHglycan	646	649	PF01048	0.545
MOD_GlcNHglycan	700	703	PF01048	0.473
MOD_GSK3_1	162	169	PF00069	0.673
MOD_GSK3_1	27	34	PF00069	0.689
MOD_GSK3_1	516	523	PF00069	0.547
MOD_GSK3_1	577	584	PF00069	0.516
MOD_GSK3_1	605	612	PF00069	0.430
MOD_GSK3_1	658	665	PF00069	0.522
MOD_GSK3_1	706	713	PF00069	0.477
MOD_N-GLC_1	206	211	PF02516	0.379
MOD_N-GLC_1	230	235	PF02516	0.393
MOD_N-GLC_1	673	678	PF02516	0.662
MOD_N-GLC_1	698	703	PF02516	0.449
MOD_NEK2_1	147	152	PF00069	0.568
MOD_NEK2_1	229	234	PF00069	0.334
MOD_NEK2_1	376	381	PF00069	0.550
MOD_NEK2_1	431	436	PF00069	0.566
MOD_NEK2_1	467	472	PF00069	0.536
MOD_NEK2_1	494	499	PF00069	0.594
MOD_NEK2_1	609	614	PF00069	0.529
MOD_NEK2_1	698	703	PF00069	0.472
MOD_NEK2_2	206	211	PF00069	0.344
MOD_OFUCOSY	178	185	PF10250	0.244
MOD_PIKK_1	354	360	PF00454	0.595
MOD_PIKK_1	662	668	PF00454	0.408
MOD_PIKK_1	806	812	PF00454	0.602
MOD_PKA_1	199	205	PF00069	0.469
MOD_PKA_2	417	423	PF00069	0.514
MOD_PKA_2	705	711	PF00069	0.642
MOD_Plk_1	166	172	PF00069	0.639
MOD_Plk_1	206	212	PF00069	0.384
MOD_Plk_1	229	235	PF00069	0.346
MOD_Plk_1	27	33	PF00069	0.693
MOD_Plk_1	494	500	PF00069	0.570
MOD_Plk_1	718	724	PF00069	0.430
MOD_Plk_1	762	768	PF00069	0.479
MOD_Plk_2-3	162	168	PF00069	0.603
MOD_Plk_4	166	172	PF00069	0.649
MOD_Plk_4	376	382	PF00069	0.532
MOD_Plk_4	431	437	PF00069	0.564
MOD_Plk_4	516	522	PF00069	0.598
MOD_Plk_4	549	555	PF00069	0.550
MOD_Plk_4	605	611	PF00069	0.536
MOD_Plk_4	762	768	PF00069	0.522
MOD_ProDKin_1	36	42	PF00069	0.641
MOD_ProDKin_1	521	527	PF00069	0.538
MOD_ProDKin_1	653	659	PF00069	0.449
MOD_SUMO_for_1	782	785	PF00179	0.487
MOD_SUMO_rev_2	555	564	PF00179	0.578
MOD_SUMO_rev_2	676	681	PF00179	0.525
TRG_ENDOCYTIC_2	100	103	PF00928	0.547
TRG_ENDOCYTIC_2	193	196	PF00928	0.365
TRG_ENDOCYTIC_2	223	226	PF00928	0.376
TRG_ENDOCYTIC_2	435	438	PF00928	0.556
TRG_ENDOCYTIC_2	464	467	PF00928	0.533
TRG_ENDOCYTIC_2	471	474	PF00928	0.531
TRG_ENDOCYTIC_2	518	521	PF00928	0.531
TRG_ENDOCYTIC_2	551	554	PF00928	0.556
TRG_ENDOCYTIC_2	678	681	PF00928	0.435
TRG_ENDOCYTIC_2	694	697	PF00928	0.450
TRG_ENDOCYTIC_2	731	734	PF00928	0.371
TRG_ER_diArg_1	121	124	PF00400	0.729
TRG_ER_diArg_1	567	570	PF00400	0.563
TRG_NLS_MonoExtN_4	198	203	PF00514	0.294
TRG_Pf-PMV_PEXEL_1	282	286	PF00026	0.651

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8T6	Leptomonas seymouri	35%	100%
A0A0N1I8N2	Leptomonas seymouri	28%	83%
A0A0N1IGQ2	Leptomonas seymouri	32%	100%
A0A0N1ILF1	Leptomonas seymouri	41%	100%
A0A0N1IMH1	Leptomonas seymouri	34%	100%
A0A0N1P9P1	Leptomonas seymouri	41%	96%
A0A0N1PCA9	Leptomonas seymouri	35%	100%
A0A0N1PE91	Leptomonas seymouri	27%	92%
A0A0N1PFI4	Leptomonas seymouri	36%	100%
A0A0S4JLK6	Bodo saltans	28%	100%
A0A0S4KGT2	Bodo saltans	40%	100%
A0A0S4KKP7	Bodo saltans	25%	100%
A0A1X0NJ19	Trypanosomatidae	30%	67%
A0A1X0NJK2	Trypanosomatidae	32%	100%
A0A1X0NJX8	Trypanosomatidae	36%	100%
A0A1X0NKT7	Trypanosomatidae	30%	100%
A0A1X0NKX8	Trypanosomatidae	32%	100%
A0A1X0NMT3	Trypanosomatidae	41%	96%
A0A1X0NW84	Trypanosomatidae	40%	100%
A0A1X0NW85	Trypanosomatidae	39%	100%
A0A1X0NW89	Trypanosomatidae	55%	95%
A0A1X0NWA6	Trypanosomatidae	29%	94%
A0A1X0NWW1	Trypanosomatidae	40%	100%
A0A3Q8IBS3	Leishmania donovani	85%	90%
A0A3Q8IDD4	Leishmania donovani	34%	100%
A0A3Q8IJT4	Leishmania donovani	25%	100%
A0A3S5H5A5	Leishmania donovani	41%	95%
A0A3S5ISG2	Trypanosoma rangeli	32%	100%
A0A3S7WW13	Leishmania donovani	27%	74%
A0A3S7WW18	Leishmania donovani	84%	99%
A0A3S7WW41	Leishmania donovani	36%	100%
A0A3S7WW71	Leishmania donovani	39%	100%
A0A3S7X430	Leishmania donovani	34%	100%
A0A3S7X438	Leishmania donovani	29%	87%
A0A3S7X460	Leishmania donovani	35%	100%
A0A3S7X463	Leishmania donovani	27%	87%
A0A3S7X470	Leishmania donovani	34%	100%
A0A422MYU1	Trypanosoma rangeli	53%	89%
A0A422MYX0	Trypanosoma rangeli	39%	100%
A4H3W4	Leishmania braziliensis	40%	100%
A4HE81	Leishmania braziliensis	34%	100%
A4HJ14	Leishmania braziliensis	35%	100%
A4HJ22	Leishmania braziliensis	30%	100%
A4HJ24	Leishmania braziliensis	35%	100%
A4HS39	Leishmania infantum	41%	95%
A4HYN0	Leishmania infantum	84%	99%
A4HYW1	Leishmania infantum	85%	80%
A4HYW2	Leishmania infantum	39%	100%
A4HYW3	Leishmania infantum	36%	100%
A4HYW4	Leishmania infantum	27%	82%
A4I1J4	Leishmania infantum	34%	100%
A4I6E4	Leishmania infantum	34%	100%
A4I6E6	Leishmania infantum	35%	100%
A4I6F0	Leishmania infantum	29%	87%
A4I6K4	Leishmania infantum	34%	100%
A4I6K5	Leishmania infantum	27%	87%
A4I6K6	Leishmania infantum	26%	100%
C9ZIE7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	51%	76%
C9ZIE8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	33%	100%
C9ZIE9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	39%	100%
C9ZN52	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	33%	100%
C9ZN53	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	33%	96%
C9ZWY4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	31%	100%
C9ZY36	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	40%	95%
E8NHG6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	100%
E8NHG7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	87%
E8NHG8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
E8NHM2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	100%
E8NHM4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
E8NHM8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
E9AIH3	Leishmania braziliensis	40%	100%
E9AIH4	Leishmania braziliensis	36%	100%
E9AIH6	Leishmania braziliensis	27%	100%
E9AK26	Leishmania mexicana (strain MHOM/GT/2001/U1103)	41%	100%
E9AUQ7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	84%	87%
E9AUQ9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	39%	100%
E9AUR0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	36%	100%
E9AUR1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	100%
E9AXM9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	100%
E9B1J0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	100%
E9B1J1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	27%	87%
E9B1J2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	100%
E9B1J6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
Q4Q6L7	Leishmania major	35%	100%
Q4Q6L9	Leishmania major	33%	100%
Q4Q6M0	Leishmania major	30%	100%
Q4Q6M2	Leishmania major	29%	100%
Q4Q6M3	Leishmania major	28%	100%
Q4Q6M4	Leishmania major	35%	100%
Q4Q9U3	Leishmania major	34%	100%
Q4QCS6	Leishmania major	36%	100%
Q4QCS7	Leishmania major	40%	100%
Q4QCS8	Leishmania major	83%	99%
Q4QCS9	Leishmania major	84%	100%
Q9U0T9	Leishmania major	41%	100%
V5AYJ1	Trypanosoma cruzi	31%	100%
V5B5I4	Trypanosoma cruzi	54%	97%
V5BA05	Trypanosoma cruzi	41%	100%
V5BEL3	Trypanosoma cruzi	36%	100%
V5BII7	Trypanosoma cruzi	30%	66%
V5BN20	Trypanosoma cruzi	36%	100%
V5D9Y2	Trypanosoma cruzi	42%	96%
V5DES7	Trypanosoma cruzi	32%	100%