Putative tubulin-tyrosine ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tubulin-tyrosine ligase, putative

Species:

Leishmania braziliensis

UniProt:

E9AID0_LEIBR

TriTrypDb:

LbrM.20.4970 , LBRM2903_200064100

Length:

748

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 19
NetGPI	no	yes: 0, no: 19

Cellular components
Term	Name	Level	Count
GO:0005929	cilium	4	2
GO:0042995	cell projection	2	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0110165	cellular anatomical entity	1	2
GO:0120025	plasma membrane bounded cell projection	3	2

Expansion

Sequence features

E9AID0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AID0

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	20
GO:0008152	metabolic process	1	20
GO:0019538	protein metabolic process	3	20
GO:0036211	protein modification process	4	20
GO:0043170	macromolecule metabolic process	3	20
GO:0043412	macromolecule modification	4	20
GO:0044238	primary metabolic process	2	20
GO:0071704	organic substance metabolic process	2	20
GO:1901564	organonitrogen compound metabolic process	3	20
GO:0000226	microtubule cytoskeleton organization	3	2
GO:0006996	organelle organization	4	2
GO:0007010	cytoskeleton organization	5	2
GO:0007017	microtubule-based process	2	2
GO:0009987	cellular process	1	2
GO:0016043	cellular component organization	3	2
GO:0018095	protein polyglutamylation	7	2
GO:0018193	peptidyl-amino acid modification	5	2
GO:0018200	peptidyl-glutamic acid modification	6	2
GO:0071840	cellular component organization or biogenesis	2	2

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	2
GO:0003824	catalytic activity	1	17
GO:0005488	binding	1	4
GO:0005524	ATP binding	5	2
GO:0016874	ligase activity	2	17
GO:0017076	purine nucleotide binding	4	2
GO:0030554	adenyl nucleotide binding	5	2
GO:0032553	ribonucleotide binding	3	2
GO:0032555	purine ribonucleotide binding	4	2
GO:0032559	adenyl ribonucleotide binding	5	2
GO:0035639	purine ribonucleoside triphosphate binding	4	2
GO:0036094	small molecule binding	2	2
GO:0043167	ion binding	2	2
GO:0043168	anion binding	3	2
GO:0043169	cation binding	3	2
GO:0046872	metal ion binding	4	2
GO:0097159	organic cyclic compound binding	2	2
GO:0097367	carbohydrate derivative binding	2	2
GO:1901265	nucleoside phosphate binding	3	2
GO:1901363	heterocyclic compound binding	2	2
GO:0004835	tubulin-tyrosine ligase activity	3	7
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	7
GO:0016881	acid-amino acid ligase activity	4	7
GO:0140096	catalytic activity, acting on a protein	2	7
GO:0005515	protein binding	2	2
GO:0008092	cytoskeletal protein binding	3	2
GO:0015631	tubulin binding	4	2
GO:0070739	protein-glutamic acid ligase activity	3	2
GO:0070740	tubulin-glutamic acid ligase activity	4	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	478	482	PF00656	0.423
CLV_C14_Caspase3-7	734	738	PF00656	0.360
CLV_NRD_NRD_1	151	153	PF00675	0.454
CLV_NRD_NRD_1	226	228	PF00675	0.286
CLV_NRD_NRD_1	239	241	PF00675	0.294
CLV_NRD_NRD_1	249	251	PF00675	0.312
CLV_NRD_NRD_1	35	37	PF00675	0.607
CLV_NRD_NRD_1	357	359	PF00675	0.157
CLV_NRD_NRD_1	403	405	PF00675	0.360
CLV_NRD_NRD_1	698	700	PF00675	0.322
CLV_NRD_NRD_1	82	84	PF00675	0.400
CLV_PCSK_KEX2_1	151	153	PF00082	0.453
CLV_PCSK_KEX2_1	208	210	PF00082	0.373
CLV_PCSK_KEX2_1	249	251	PF00082	0.328
CLV_PCSK_KEX2_1	35	37	PF00082	0.538
CLV_PCSK_KEX2_1	698	700	PF00082	0.320
CLV_PCSK_KEX2_1	81	83	PF00082	0.508
CLV_PCSK_PC1ET2_1	208	210	PF00082	0.377
CLV_PCSK_SKI1_1	194	198	PF00082	0.496
CLV_PCSK_SKI1_1	208	212	PF00082	0.537
CLV_PCSK_SKI1_1	38	42	PF00082	0.544
CLV_PCSK_SKI1_1	524	528	PF00082	0.242
CLV_PCSK_SKI1_1	535	539	PF00082	0.237
CLV_PCSK_SKI1_1	609	613	PF00082	0.320
CLV_PCSK_SKI1_1	654	658	PF00082	0.646
CLV_PCSK_SKI1_1	93	97	PF00082	0.533
CLV_Separin_Metazoa	521	525	PF03568	0.507
DEG_APCC_DBOX_1	425	433	PF00400	0.507
DEG_APCC_DBOX_1	608	616	PF00400	0.507
DEG_SPOP_SBC_1	353	357	PF00917	0.357
DEG_SPOP_SBC_1	54	58	PF00917	0.567
DOC_CYCLIN_yCln2_LP_2	646	649	PF00134	0.303
DOC_MAPK_gen_1	240	248	PF00069	0.456
DOC_MAPK_gen_1	404	412	PF00069	0.567
DOC_MAPK_gen_1	715	722	PF00069	0.592
DOC_MAPK_gen_1	81	88	PF00069	0.375
DOC_MAPK_JIP1_4	609	615	PF00069	0.507
DOC_MAPK_MEF2A_6	240	248	PF00069	0.401
DOC_MAPK_MEF2A_6	404	412	PF00069	0.428
DOC_MAPK_MEF2A_6	518	527	PF00069	0.444
DOC_MAPK_MEF2A_6	609	617	PF00069	0.492
DOC_MAPK_MEF2A_6	81	88	PF00069	0.435
DOC_MAPK_NFAT4_5	241	249	PF00069	0.357
DOC_MAPK_NFAT4_5	405	413	PF00069	0.429
DOC_PP1_RVXF_1	522	528	PF00149	0.416
DOC_PP1_RVXF_1	652	658	PF00149	0.421
DOC_PP2B_LxvP_1	646	649	PF13499	0.303
DOC_PP4_MxPP_1	647	650	PF00568	0.326
DOC_USP7_MATH_1	139	143	PF00917	0.696
DOC_USP7_MATH_1	155	159	PF00917	0.482
DOC_USP7_MATH_1	17	21	PF00917	0.482
DOC_USP7_MATH_1	305	309	PF00917	0.539
DOC_USP7_MATH_1	370	374	PF00917	0.566
DOC_USP7_MATH_1	452	456	PF00917	0.422
DOC_USP7_MATH_1	475	479	PF00917	0.430
DOC_USP7_MATH_1	489	493	PF00917	0.475
DOC_USP7_MATH_1	561	565	PF00917	0.548
DOC_USP7_MATH_1	731	735	PF00917	0.550
DOC_WW_Pin1_4	171	176	PF00397	0.545
DOC_WW_Pin1_4	282	287	PF00397	0.507
DOC_WW_Pin1_4	309	314	PF00397	0.514
DOC_WW_Pin1_4	44	49	PF00397	0.458
DOC_WW_Pin1_4	528	533	PF00397	0.507
DOC_WW_Pin1_4	570	575	PF00397	0.381
DOC_WW_Pin1_4	725	730	PF00397	0.648
DOC_WW_Pin1_4	74	79	PF00397	0.337
LIG_14-3-3_CanoR_1	156	162	PF00244	0.527
LIG_14-3-3_CanoR_1	164	168	PF00244	0.314
LIG_14-3-3_CanoR_1	332	340	PF00244	0.391
LIG_14-3-3_CanoR_1	35	45	PF00244	0.482
LIG_14-3-3_CanoR_1	431	436	PF00244	0.418
LIG_14-3-3_CanoR_1	439	448	PF00244	0.418
LIG_14-3-3_CanoR_1	539	546	PF00244	0.560
LIG_14-3-3_CanoR_1	710	716	PF00244	0.302
LIG_Actin_WH2_2	454	469	PF00022	0.357
LIG_Actin_WH2_2	523	541	PF00022	0.412
LIG_Actin_WH2_2	594	611	PF00022	0.374
LIG_APCC_ABBA_1	112	117	PF00400	0.439
LIG_APCC_ABBA_1	422	427	PF00400	0.466
LIG_APCC_ABBAyCdc20_2	421	427	PF00400	0.431
LIG_BIR_II_1	1	5	PF00653	0.490
LIG_BRCT_BRCA1_1	311	315	PF00533	0.357
LIG_BRCT_BRCA1_1	572	576	PF00533	0.507
LIG_EH_1	396	400	PF12763	0.412
LIG_EH1_1	578	586	PF00400	0.412
LIG_FHA_1	175	181	PF00498	0.353
LIG_FHA_1	199	205	PF00498	0.388
LIG_FHA_1	233	239	PF00498	0.441
LIG_FHA_1	361	367	PF00498	0.426
LIG_FHA_1	529	535	PF00498	0.525
LIG_FHA_1	657	663	PF00498	0.683
LIG_FHA_1	702	708	PF00498	0.460
LIG_FHA_2	105	111	PF00498	0.450
LIG_FHA_2	413	419	PF00498	0.507
LIG_FHA_2	448	454	PF00498	0.382
LIG_FHA_2	57	63	PF00498	0.621
LIG_FHA_2	66	72	PF00498	0.402
LIG_FHA_2	691	697	PF00498	0.349
LIG_FHA_2	94	100	PF00498	0.521
LIG_LIR_Apic_2	287	292	PF02991	0.457
LIG_LIR_Apic_2	507	513	PF02991	0.365
LIG_LIR_Gen_1	174	183	PF02991	0.385
LIG_LIR_Gen_1	573	584	PF02991	0.412
LIG_LIR_Gen_1	622	632	PF02991	0.412
LIG_LIR_Gen_1	701	708	PF02991	0.382
LIG_LIR_Nem_3	174	179	PF02991	0.418
LIG_LIR_Nem_3	223	229	PF02991	0.270
LIG_LIR_Nem_3	415	420	PF02991	0.470
LIG_LIR_Nem_3	572	578	PF02991	0.439
LIG_LIR_Nem_3	622	628	PF02991	0.416
LIG_LIR_Nem_3	701	706	PF02991	0.374
LIG_LIR_Nem_3	714	719	PF02991	0.426
LIG_LIR_Nem_3	85	91	PF02991	0.430
LIG_MLH1_MIPbox_1	572	576	PF16413	0.412
LIG_NRBOX	243	249	PF00104	0.412
LIG_PCNA_PIPBox_1	618	627	PF02747	0.424
LIG_PCNA_yPIPBox_3	386	395	PF02747	0.387
LIG_Pex14_2	575	579	PF04695	0.507
LIG_SH2_CRK	162	166	PF00017	0.337
LIG_SH2_CRK	226	230	PF00017	0.540
LIG_SH2_CRK	417	421	PF00017	0.546
LIG_SH2_CRK	716	720	PF00017	0.376
LIG_SH2_CRK	91	95	PF00017	0.465
LIG_SH2_NCK_1	673	677	PF00017	0.411
LIG_SH2_NCK_1	91	95	PF00017	0.486
LIG_SH2_PTP2	411	414	PF00017	0.406
LIG_SH2_SRC	417	420	PF00017	0.452
LIG_SH2_STAT3	389	392	PF00017	0.459
LIG_SH2_STAT5	167	170	PF00017	0.401
LIG_SH2_STAT5	389	392	PF00017	0.476
LIG_SH2_STAT5	411	414	PF00017	0.437
LIG_SH2_STAT5	423	426	PF00017	0.458
LIG_SH2_STAT5	437	440	PF00017	0.494
LIG_SH2_STAT5	504	507	PF00017	0.471
LIG_SH2_STAT5	73	76	PF00017	0.436
LIG_SH3_3	123	129	PF00018	0.481
LIG_SH3_3	48	54	PF00018	0.657
LIG_SH3_3	645	651	PF00018	0.313
LIG_SH3_3	72	78	PF00018	0.441
LIG_SUMO_SIM_anti_2	582	592	PF11976	0.507
LIG_SUMO_SIM_anti_2	639	645	PF11976	0.396
LIG_SUMO_SIM_par_1	582	592	PF11976	0.507
LIG_SUMO_SIM_par_1	597	602	PF11976	0.537
LIG_SUMO_SIM_par_1	609	614	PF11976	0.526
LIG_TRAF2_1	692	695	PF00917	0.286
LIG_TYR_ITSM	413	420	PF00017	0.412
LIG_WW_2	650	653	PF00397	0.345
MOD_CDK_SPxxK_3	528	535	PF00069	0.507
MOD_CDK_SPxxK_3	74	81	PF00069	0.477
MOD_CK1_1	134	140	PF00069	0.705
MOD_CK1_1	163	169	PF00069	0.391
MOD_CK1_1	19	25	PF00069	0.453
MOD_CK1_1	354	360	PF00069	0.441
MOD_CK1_1	365	371	PF00069	0.471
MOD_CK1_1	497	503	PF00069	0.439
MOD_CK1_1	541	547	PF00069	0.421
MOD_CK1_1	548	554	PF00069	0.452
MOD_CK1_1	55	61	PF00069	0.731
MOD_CK1_1	565	571	PF00069	0.528
MOD_CK1_1	604	610	PF00069	0.462
MOD_CK1_1	620	626	PF00069	0.443
MOD_CK1_1	668	674	PF00069	0.358
MOD_CK2_1	104	110	PF00069	0.583
MOD_CK2_1	354	360	PF00069	0.409
MOD_CK2_1	36	42	PF00069	0.372
MOD_CK2_1	57	63	PF00069	0.603
MOD_CK2_1	690	696	PF00069	0.305
MOD_CK2_1	93	99	PF00069	0.655
MOD_Cter_Amidation	238	241	PF01082	0.231
MOD_Cter_Amidation	402	405	PF01082	0.331
MOD_GlcNHglycan	1	4	PF01048	0.639
MOD_GlcNHglycan	133	136	PF01048	0.728
MOD_GlcNHglycan	141	144	PF01048	0.784
MOD_GlcNHglycan	157	160	PF01048	0.650
MOD_GlcNHglycan	191	194	PF01048	0.470
MOD_GlcNHglycan	257	260	PF01048	0.293
MOD_GlcNHglycan	271	274	PF01048	0.354
MOD_GlcNHglycan	307	310	PF01048	0.349
MOD_GlcNHglycan	367	371	PF01048	0.205
MOD_GlcNHglycan	38	41	PF01048	0.370
MOD_GlcNHglycan	541	544	PF01048	0.289
MOD_GlcNHglycan	547	550	PF01048	0.314
MOD_GlcNHglycan	601	604	PF01048	0.208
MOD_GlcNHglycan	62	66	PF01048	0.523
MOD_GlcNHglycan	711	714	PF01048	0.508
MOD_GSK3_1	100	107	PF00069	0.683
MOD_GSK3_1	255	262	PF00069	0.497
MOD_GSK3_1	265	272	PF00069	0.445
MOD_GSK3_1	305	312	PF00069	0.446
MOD_GSK3_1	362	369	PF00069	0.486
MOD_GSK3_1	52	59	PF00069	0.634
MOD_GSK3_1	541	548	PF00069	0.447
MOD_GSK3_1	561	568	PF00069	0.539
MOD_GSK3_1	61	68	PF00069	0.504
MOD_GSK3_1	619	626	PF00069	0.450
MOD_GSK3_1	727	734	PF00069	0.635
MOD_GSK3_1	739	746	PF00069	0.473
MOD_N-GLC_1	171	176	PF02516	0.264
MOD_N-GLC_1	259	264	PF02516	0.233
MOD_N-GLC_1	462	467	PF02516	0.259
MOD_N-GLC_1	489	494	PF02516	0.157
MOD_N-GLC_1	569	574	PF02516	0.187
MOD_N-GLC_1	663	668	PF02516	0.708
MOD_N-GLC_1	701	706	PF02516	0.346
MOD_N-GLC_1	725	730	PF02516	0.437
MOD_N-GLC_2	688	690	PF02516	0.300
MOD_NEK2_1	144	149	PF00069	0.519
MOD_NEK2_1	179	184	PF00069	0.353
MOD_NEK2_1	198	203	PF00069	0.254
MOD_NEK2_1	265	270	PF00069	0.366
MOD_NEK2_1	366	371	PF00069	0.404
MOD_NEK2_1	538	543	PF00069	0.513
MOD_NEK2_1	599	604	PF00069	0.416
MOD_NEK2_2	381	386	PF00069	0.357
MOD_PIKK_1	144	150	PF00454	0.506
MOD_PIKK_1	665	671	PF00454	0.526
MOD_PKA_1	82	88	PF00069	0.365
MOD_PKA_2	104	110	PF00069	0.530
MOD_PKA_2	155	161	PF00069	0.583
MOD_PKA_2	163	169	PF00069	0.320
MOD_PKA_2	189	195	PF00069	0.520
MOD_PKA_2	331	337	PF00069	0.412
MOD_PKA_2	438	444	PF00069	0.382
MOD_PKA_2	538	544	PF00069	0.564
MOD_PKA_2	709	715	PF00069	0.508
MOD_PKA_2	82	88	PF00069	0.409
MOD_Plk_1	108	114	PF00069	0.627
MOD_Plk_1	366	372	PF00069	0.375
MOD_Plk_1	462	468	PF00069	0.480
MOD_Plk_1	701	707	PF00069	0.466
MOD_Plk_4	163	169	PF00069	0.393
MOD_Plk_4	200	206	PF00069	0.400
MOD_Plk_4	210	216	PF00069	0.523
MOD_Plk_4	285	291	PF00069	0.576
MOD_Plk_4	338	344	PF00069	0.426
MOD_Plk_4	46	52	PF00069	0.629
MOD_Plk_4	468	474	PF00069	0.423
MOD_Plk_4	623	629	PF00069	0.411
MOD_Plk_4	639	645	PF00069	0.477
MOD_Plk_4	739	745	PF00069	0.357
MOD_ProDKin_1	171	177	PF00069	0.539
MOD_ProDKin_1	282	288	PF00069	0.507
MOD_ProDKin_1	309	315	PF00069	0.514
MOD_ProDKin_1	44	50	PF00069	0.459
MOD_ProDKin_1	528	534	PF00069	0.507
MOD_ProDKin_1	570	576	PF00069	0.381
MOD_ProDKin_1	725	731	PF00069	0.650
MOD_ProDKin_1	74	80	PF00069	0.347
MOD_SUMO_rev_2	492	497	PF00179	0.410
TRG_DiLeu_BaEn_1	521	526	PF01217	0.452
TRG_DiLeu_BaLyEn_6	206	211	PF01217	0.271
TRG_DiLeu_BaLyEn_6	702	707	PF01217	0.465
TRG_ENDOCYTIC_2	162	165	PF00928	0.370
TRG_ENDOCYTIC_2	226	229	PF00928	0.549
TRG_ENDOCYTIC_2	411	414	PF00928	0.452
TRG_ENDOCYTIC_2	417	420	PF00928	0.465
TRG_ENDOCYTIC_2	625	628	PF00928	0.416
TRG_ENDOCYTIC_2	716	719	PF00928	0.337
TRG_ER_diArg_1	151	153	PF00400	0.469
TRG_ER_diArg_1	248	250	PF00400	0.527
TRG_ER_diArg_1	35	38	PF00400	0.605
TRG_ER_diArg_1	81	83	PF00400	0.411
TRG_NES_CRM1_1	521	533	PF08389	0.412
TRG_NES_CRM1_1	577	589	PF08389	0.412
TRG_Pf-PMV_PEXEL_1	300	304	PF00026	0.212
TRG_Pf-PMV_PEXEL_1	717	721	PF00026	0.507

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P7G7	Leptomonas seymouri	44%	91%
A0A0S4IZZ2	Bodo saltans	28%	84%
A0A0S4JH38	Bodo saltans	22%	100%
A0A0S4KQK8	Bodo saltans	28%	100%
A0A1X0NXX8	Trypanosomatidae	31%	100%
A0A3Q8IDL7	Leishmania donovani	75%	100%
A0A3Q8IEC1	Leishmania donovani	28%	100%
A0A3Q8III2	Leishmania donovani	28%	100%
A0A3S5H6X0	Leishmania donovani	24%	92%
A0A3S7X261	Leishmania donovani	25%	100%
A0A422NPL9	Trypanosoma rangeli	34%	100%
A4H8G3	Leishmania braziliensis	25%	100%
A4HG29	Leishmania braziliensis	24%	100%
A4HIG0	Leishmania braziliensis	25%	100%
A4HNF6	Leishmania braziliensis	28%	100%
A4HWT7	Leishmania infantum	24%	92%
A4HYN9	Leishmania infantum	76%	100%
A4I479	Leishmania infantum	24%	100%
A4I5Q6	Leishmania infantum	27%	100%
A4IC28	Leishmania infantum	28%	100%
A4Q9F0	Mus musculus	22%	82%
A8X9V4	Caenorhabditis briggsae	23%	100%
C9ZI96	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	34%	100%
E9ADM8	Leishmania major	24%	100%
E9AFW7	Leishmania major	28%	100%
E9AM52	Leishmania mexicana (strain MHOM/GT/2001/U1103)	24%	100%
E9AUL7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	76%	100%
E9B720	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	100%
E9P886	Caenorhabditis elegans	27%	100%
Q09647	Caenorhabditis elegans	24%	100%
Q4QCW7	Leishmania major	76%	100%
Q6ZT98	Homo sapiens	24%	84%
V5BVF5	Trypanosoma cruzi	33%	100%
V5C123	Trypanosoma cruzi	22%	79%