Checkpoint protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Checkpoint protein

Gene product:

Cell cycle checkpoint protein RAD1-like, putative

Species:

Leishmania braziliensis

UniProt:

E9AI98_LEIBR

TriTrypDb:

LbrM.20.4640 , LBRM2903_200060200

Length:

357

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	11
GO:0043226	organelle	2	11
GO:0043227	membrane-bounded organelle	3	11
GO:0043229	intracellular organelle	3	11
GO:0043231	intracellular membrane-bounded organelle	4	11
GO:0110165	cellular anatomical entity	1	11
GO:0030896	checkpoint clamp complex	3	1
GO:0032991	protein-containing complex	1	1
GO:0140513	nuclear protein-containing complex	2	1

Expansion

Sequence features

E9AI98

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AI98

Function

Biological processes
Term	Name	Level	Count
GO:0000075	cell cycle checkpoint signaling	4	11
GO:0000077	DNA damage checkpoint signaling	5	11
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006259	DNA metabolic process	4	11
GO:0006281	DNA repair	5	11
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0006950	response to stress	2	11
GO:0006974	DNA damage response	4	11
GO:0007165	signal transduction	2	11
GO:0008152	metabolic process	1	11
GO:0009987	cellular process	1	11
GO:0010564	regulation of cell cycle process	5	11
GO:0010948	negative regulation of cell cycle process	6	11
GO:0031570	DNA integrity checkpoint signaling	5	11
GO:0033554	cellular response to stress	3	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0035556	intracellular signal transduction	3	11
GO:0042770	signal transduction in response to DNA damage	4	11
GO:0043170	macromolecule metabolic process	3	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044260	obsolete cellular macromolecule metabolic process	3	11
GO:0045786	negative regulation of cell cycle	5	11
GO:0046483	heterocycle metabolic process	3	11
GO:0048519	negative regulation of biological process	3	11
GO:0048523	negative regulation of cellular process	4	11
GO:0050789	regulation of biological process	2	11
GO:0050794	regulation of cellular process	3	11
GO:0050896	response to stimulus	1	11
GO:0051716	cellular response to stimulus	2	11
GO:0051726	regulation of cell cycle	4	11
GO:0065007	biological regulation	1	11
GO:0071704	organic substance metabolic process	2	11
GO:0090304	nucleic acid metabolic process	4	11
GO:1901360	organic cyclic compound metabolic process	3	11
GO:1901987	regulation of cell cycle phase transition	6	11
GO:1901988	negative regulation of cell cycle phase transition	7	11

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	1
GO:0004518	nuclease activity	4	1
GO:0004527	exonuclease activity	5	1
GO:0004529	DNA exonuclease activity	5	1
GO:0004536	DNA nuclease activity	4	1
GO:0008853	obsolete exodeoxyribonuclease III activity	7	1
GO:0016787	hydrolase activity	2	1
GO:0016788	hydrolase activity, acting on ester bonds	3	1
GO:0016796	exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters	6	1
GO:0016895	DNA exonuclease activity, producing 5'-phosphomonoesters	6	1
GO:0140097	catalytic activity, acting on DNA	3	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	275	279	PF00656	0.700
CLV_C14_Caspase3-7	75	79	PF00656	0.311
CLV_MEL_PAP_1	148	154	PF00089	0.357
CLV_PCSK_SKI1_1	56	60	PF00082	0.322
CLV_PCSK_SKI1_1	68	72	PF00082	0.267
CLV_PCSK_SKI1_1	93	97	PF00082	0.414
DEG_APCC_DBOX_1	143	151	PF00400	0.355
DOC_ANK_TNKS_1	264	271	PF00023	0.516
DOC_CYCLIN_RxL_1	53	61	PF00134	0.357
DOC_MAPK_gen_1	141	150	PF00069	0.298
DOC_MAPK_HePTP_8	183	195	PF00069	0.355
DOC_MAPK_MEF2A_6	144	152	PF00069	0.297
DOC_MAPK_MEF2A_6	186	195	PF00069	0.355
DOC_MAPK_MEF2A_6	335	343	PF00069	0.448
DOC_MAPK_MEF2A_6	6	14	PF00069	0.345
DOC_PP2B_LxvP_1	71	74	PF13499	0.386
DOC_USP7_MATH_1	298	302	PF00917	0.652
DOC_USP7_MATH_1	349	353	PF00917	0.528
DOC_USP7_MATH_1	88	92	PF00917	0.355
DOC_WW_Pin1_4	107	112	PF00397	0.410
DOC_WW_Pin1_4	117	122	PF00397	0.410
DOC_WW_Pin1_4	6	11	PF00397	0.465
LIG_14-3-3_CanoR_1	132	138	PF00244	0.410
LIG_14-3-3_CanoR_1	15	21	PF00244	0.410
LIG_14-3-3_CanoR_1	151	158	PF00244	0.410
LIG_14-3-3_CanoR_1	223	230	PF00244	0.333
LIG_14-3-3_CanoR_1	280	289	PF00244	0.606
LIG_14-3-3_CanoR_1	317	321	PF00244	0.392
LIG_14-3-3_CanoR_1	326	331	PF00244	0.307
LIG_Actin_WH2_2	136	153	PF00022	0.410
LIG_Actin_WH2_2	324	342	PF00022	0.316
LIG_APCC_ABBA_1	37	42	PF00400	0.297
LIG_BIR_II_1	1	5	PF00653	0.358
LIG_deltaCOP1_diTrp_1	25	32	PF00928	0.279
LIG_FHA_1	138	144	PF00498	0.297
LIG_FHA_1	17	23	PF00498	0.373
LIG_FHA_1	229	235	PF00498	0.361
LIG_FHA_1	282	288	PF00498	0.421
LIG_FHA_1	317	323	PF00498	0.451
LIG_FHA_1	342	348	PF00498	0.369
LIG_FHA_1	49	55	PF00498	0.337
LIG_FHA_1	90	96	PF00498	0.343
LIG_FHA_2	108	114	PF00498	0.297
LIG_FHA_2	120	126	PF00498	0.150
LIG_FHA_2	73	79	PF00498	0.322
LIG_LIR_Gen_1	2	12	PF02991	0.342
LIG_LIR_Gen_1	25	32	PF02991	0.279
LIG_LIR_Gen_1	284	293	PF02991	0.667
LIG_LIR_Gen_1	351	357	PF02991	0.595
LIG_LIR_Gen_1	61	70	PF02991	0.410
LIG_LIR_Nem_3	2	7	PF02991	0.332
LIG_LIR_Nem_3	25	30	PF02991	0.279
LIG_LIR_Nem_3	310	315	PF02991	0.445
LIG_LIR_Nem_3	351	357	PF02991	0.595
LIG_LIR_Nem_3	61	65	PF02991	0.346
LIG_NRBOX	133	139	PF00104	0.410
LIG_NRBOX	190	196	PF00104	0.197
LIG_PDZ_Class_3	352	357	PF00595	0.611
LIG_PTB_Apo_2	280	287	PF02174	0.574
LIG_PTB_Phospho_1	280	286	PF10480	0.576
LIG_SH2_CRK	4	8	PF00017	0.344
LIG_SH2_NCK_1	62	66	PF00017	0.410
LIG_SH2_STAP1	230	234	PF00017	0.483
LIG_SH2_STAT5	230	233	PF00017	0.488
LIG_SH2_STAT5	286	289	PF00017	0.679
LIG_SH2_STAT5	4	7	PF00017	0.322
LIG_SH3_3	343	349	PF00018	0.357
LIG_SUMO_SIM_anti_2	342	347	PF11976	0.406
LIG_SUMO_SIM_par_1	105	110	PF11976	0.279
LIG_SUMO_SIM_par_1	18	23	PF11976	0.322
LIG_SUMO_SIM_par_1	213	220	PF11976	0.408
MOD_CDK_SPxxK_3	107	114	PF00069	0.355
MOD_CK1_1	224	230	PF00069	0.330
MOD_CK1_1	251	257	PF00069	0.747
MOD_CK1_1	86	92	PF00069	0.373
MOD_GlcNHglycan	222	226	PF01048	0.365
MOD_GlcNHglycan	250	253	PF01048	0.675
MOD_GlcNHglycan	293	296	PF01048	0.720
MOD_GlcNHglycan	300	303	PF01048	0.491
MOD_GSK3_1	117	124	PF00069	0.322
MOD_GSK3_1	133	140	PF00069	0.313
MOD_GSK3_1	16	23	PF00069	0.338
MOD_GSK3_1	217	224	PF00069	0.322
MOD_GSK3_1	247	254	PF00069	0.755
MOD_GSK3_1	26	33	PF00069	0.251
MOD_GSK3_1	46	53	PF00069	0.150
MOD_N-GLC_1	46	51	PF02516	0.410
MOD_NEK2_1	133	138	PF00069	0.302
MOD_NEK2_1	264	269	PF00069	0.803
MOD_NEK2_1	291	296	PF00069	0.613
MOD_NEK2_1	32	37	PF00069	0.355
MOD_NEK2_1	339	344	PF00069	0.298
MOD_NEK2_1	48	53	PF00069	0.322
MOD_NEK2_2	307	312	PF00069	0.590
MOD_NEK2_2	349	354	PF00069	0.515
MOD_PK_1	326	332	PF00069	0.363
MOD_PKA_2	150	156	PF00069	0.355
MOD_PKA_2	264	270	PF00069	0.781
MOD_PKA_2	279	285	PF00069	0.670
MOD_PKA_2	316	322	PF00069	0.457
MOD_PKB_1	221	229	PF00069	0.197
MOD_Plk_1	181	187	PF00069	0.410
MOD_Plk_4	133	139	PF00069	0.360
MOD_Plk_4	26	32	PF00069	0.318
MOD_Plk_4	307	313	PF00069	0.581
MOD_Plk_4	326	332	PF00069	0.357
MOD_Plk_4	341	347	PF00069	0.378
MOD_Plk_4	349	355	PF00069	0.519
MOD_Plk_4	72	78	PF00069	0.484
MOD_ProDKin_1	107	113	PF00069	0.410
MOD_ProDKin_1	117	123	PF00069	0.410
MOD_ProDKin_1	6	12	PF00069	0.463
MOD_SUMO_rev_2	101	106	PF00179	0.436
MOD_SUMO_rev_2	182	191	PF00179	0.355
MOD_SUMO_rev_2	336	342	PF00179	0.566
TRG_ENDOCYTIC_2	202	205	PF00928	0.322
TRG_ENDOCYTIC_2	286	289	PF00928	0.667
TRG_ENDOCYTIC_2	354	357	PF00928	0.606
TRG_ENDOCYTIC_2	4	7	PF00928	0.322
TRG_ENDOCYTIC_2	62	65	PF00928	0.410
TRG_Pf-PMV_PEXEL_1	104	108	PF00026	0.279
TRG_Pf-PMV_PEXEL_1	169	174	PF00026	0.410

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HUB9	Leptomonas seymouri	70%	100%
A0A1X0NWX4	Trypanosomatidae	46%	100%
A0A3S7WVU5	Leishmania donovani	86%	99%
A0A422NCM6	Trypanosoma rangeli	45%	100%
A4HYN1	Leishmania infantum	91%	100%
C9ZI59	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	39%	100%
E9AUI3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	97%
Q4QD01	Leishmania major	87%	99%
V5BVI1	Trypanosoma cruzi	44%	100%