ZZ-type domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

E3 ubiquitin-protein ligase KCMF1, putative

Species:

Leishmania braziliensis

UniProt:

E9AI85_LEIBR

TriTrypDb:

LbrM.20.4510 , LBRM2903_200058800

Length:

784

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005622	intracellular anatomical structure	2	1
GO:0005737	cytoplasm	2	1
GO:0005773	vacuole	5	1
GO:0005776	autophagosome	6	1
GO:0016234	inclusion body	3	1
GO:0016235	aggresome	4	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0043233	organelle lumen	3	1
GO:0044753	amphisome	7	1
GO:0070013	intracellular organelle lumen	4	1
GO:0097014	ciliary plasm	5	1
GO:0099568	cytoplasmic region	3	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AI85

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AI85

Function

Biological processes
Term	Name	Level	Count
GO:0000422	autophagy of mitochondrion	4	1
GO:0000423	mitophagy	5	1
GO:0006914	autophagy	3	1
GO:0006996	organelle organization	4	1
GO:0007005	mitochondrion organization	5	1
GO:0007032	endosome organization	6	1
GO:0008152	metabolic process	1	1
GO:0009056	catabolic process	2	1
GO:0009987	cellular process	1	1
GO:0016043	cellular component organization	3	1
GO:0016050	vesicle organization	5	1
GO:0016236	macroautophagy	4	1
GO:0022411	cellular component disassembly	4	1
GO:0035973	aggrephagy	6	1
GO:0044237	cellular metabolic process	2	1
GO:0044248	cellular catabolic process	3	1
GO:0061726	mitochondrion disassembly	6	1
GO:0061912	selective autophagy	5	1
GO:0061919	process utilizing autophagic mechanism	2	1
GO:0071840	cellular component organization or biogenesis	2	1
GO:1903008	organelle disassembly	5	1

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	11
GO:0008270	zinc ion binding	6	11
GO:0043167	ion binding	2	11
GO:0043169	cation binding	3	11
GO:0046872	metal ion binding	4	11
GO:0046914	transition metal ion binding	5	11
GO:0005080	protein kinase C binding	6	1
GO:0005515	protein binding	2	1
GO:0019899	enzyme binding	3	1
GO:0019900	kinase binding	4	1
GO:0019901	protein kinase binding	5	1
GO:0031593	polyubiquitin modification-dependent protein binding	4	1
GO:0070530	K63-linked polyubiquitin modification-dependent protein binding	5	1
GO:0140030	modification-dependent protein binding	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	713	717	PF00656	0.593
CLV_NRD_NRD_1	397	399	PF00675	0.506
CLV_NRD_NRD_1	415	417	PF00675	0.560
CLV_NRD_NRD_1	445	447	PF00675	0.642
CLV_NRD_NRD_1	485	487	PF00675	0.590
CLV_NRD_NRD_1	541	543	PF00675	0.557
CLV_NRD_NRD_1	609	611	PF00675	0.522
CLV_NRD_NRD_1	71	73	PF00675	0.477
CLV_PCSK_FUR_1	443	447	PF00082	0.647
CLV_PCSK_KEX2_1	326	328	PF00082	0.361
CLV_PCSK_KEX2_1	37	39	PF00082	0.421
CLV_PCSK_KEX2_1	397	399	PF00082	0.559
CLV_PCSK_KEX2_1	415	417	PF00082	0.546
CLV_PCSK_KEX2_1	443	445	PF00082	0.755
CLV_PCSK_KEX2_1	485	487	PF00082	0.509
CLV_PCSK_KEX2_1	541	543	PF00082	0.464
CLV_PCSK_KEX2_1	609	611	PF00082	0.340
CLV_PCSK_KEX2_1	71	73	PF00082	0.438
CLV_PCSK_PC1ET2_1	326	328	PF00082	0.361
CLV_PCSK_PC1ET2_1	37	39	PF00082	0.421
CLV_PCSK_PC7_1	393	399	PF00082	0.387
CLV_PCSK_SKI1_1	266	270	PF00082	0.443
CLV_PCSK_SKI1_1	343	347	PF00082	0.500
CLV_PCSK_SKI1_1	610	614	PF00082	0.403
CLV_PCSK_SKI1_1	71	75	PF00082	0.454
CLV_PCSK_SKI1_1	84	88	PF00082	0.293
DEG_APCC_DBOX_1	158	166	PF00400	0.394
DEG_APCC_DBOX_1	235	243	PF00400	0.466
DEG_APCC_DBOX_1	396	404	PF00400	0.466
DEG_APCC_DBOX_1	609	617	PF00400	0.287
DEG_APCC_DBOX_1	83	91	PF00400	0.358
DEG_SCF_FBW7_1	558	564	PF00400	0.399
DEG_SCF_FBW7_1	736	743	PF00400	0.490
DEG_SPOP_SBC_1	191	195	PF00917	0.475
DOC_CKS1_1	123	128	PF01111	0.500
DOC_CKS1_1	170	175	PF01111	0.444
DOC_CKS1_1	558	563	PF01111	0.531
DOC_CYCLIN_RxL_1	263	272	PF00134	0.499
DOC_CYCLIN_RxL_1	481	492	PF00134	0.508
DOC_CYCLIN_yCln2_LP_2	120	126	PF00134	0.432
DOC_MAPK_FxFP_2	666	669	PF00069	0.426
DOC_MAPK_gen_1	326	333	PF00069	0.348
DOC_MAPK_gen_1	485	491	PF00069	0.479
DOC_MAPK_MEF2A_6	326	335	PF00069	0.322
DOC_MAPK_MEF2A_6	354	363	PF00069	0.293
DOC_PP1_RVXF_1	680	687	PF00149	0.289
DOC_PP2B_LxvP_1	631	634	PF13499	0.465
DOC_PP2B_LxvP_1	755	758	PF13499	0.514
DOC_PP4_FxxP_1	666	669	PF00568	0.426
DOC_PP4_FxxP_1	768	771	PF00568	0.531
DOC_USP7_MATH_1	192	196	PF00917	0.571
DOC_USP7_MATH_1	420	424	PF00917	0.460
DOC_USP7_MATH_1	561	565	PF00917	0.538
DOC_USP7_MATH_1	582	586	PF00917	0.415
DOC_USP7_MATH_1	658	662	PF00917	0.533
DOC_USP7_MATH_1	735	739	PF00917	0.649
DOC_USP7_MATH_1	771	775	PF00917	0.511
DOC_WW_Pin1_4	122	127	PF00397	0.435
DOC_WW_Pin1_4	169	174	PF00397	0.542
DOC_WW_Pin1_4	355	360	PF00397	0.397
DOC_WW_Pin1_4	436	441	PF00397	0.625
DOC_WW_Pin1_4	557	562	PF00397	0.470
DOC_WW_Pin1_4	729	734	PF00397	0.636
DOC_WW_Pin1_4	736	741	PF00397	0.630
LIG_14-3-3_CanoR_1	208	217	PF00244	0.414
LIG_14-3-3_CanoR_1	236	240	PF00244	0.353
LIG_14-3-3_CanoR_1	266	274	PF00244	0.454
LIG_14-3-3_CanoR_1	408	417	PF00244	0.552
LIG_14-3-3_CanoR_1	665	669	PF00244	0.598
LIG_14-3-3_CanoR_1	676	681	PF00244	0.384
LIG_14-3-3_CanoR_1	702	709	PF00244	0.569
LIG_14-3-3_CanoR_1	95	100	PF00244	0.393
LIG_Actin_WH2_2	29	47	PF00022	0.430
LIG_AP2alpha_2	294	296	PF02296	0.348
LIG_APCC_ABBA_1	654	659	PF00400	0.483
LIG_BIR_II_1	1	5	PF00653	0.578
LIG_BIR_III_2	391	395	PF00653	0.311
LIG_Clathr_ClatBox_1	332	336	PF01394	0.322
LIG_Clathr_ClatBox_1	380	384	PF01394	0.428
LIG_deltaCOP1_diTrp_1	262	269	PF00928	0.488
LIG_deltaCOP1_diTrp_1	294	299	PF00928	0.357
LIG_deltaCOP1_diTrp_1	567	573	PF00928	0.418
LIG_DLG_GKlike_1	676	684	PF00625	0.324
LIG_EVH1_1	307	311	PF00568	0.360
LIG_FHA_1	105	111	PF00498	0.480
LIG_FHA_1	123	129	PF00498	0.260
LIG_FHA_1	209	215	PF00498	0.599
LIG_FHA_1	278	284	PF00498	0.595
LIG_FHA_1	313	319	PF00498	0.568
LIG_FHA_1	424	430	PF00498	0.626
LIG_FHA_1	451	457	PF00498	0.678
LIG_FHA_1	490	496	PF00498	0.384
LIG_FHA_1	558	564	PF00498	0.512
LIG_FHA_1	588	594	PF00498	0.490
LIG_FHA_1	68	74	PF00498	0.455
LIG_FHA_1	737	743	PF00498	0.705
LIG_FHA_2	176	182	PF00498	0.610
LIG_FHA_2	342	348	PF00498	0.558
LIG_FHA_2	527	533	PF00498	0.474
LIG_IBAR_NPY_1	503	505	PF08397	0.491
LIG_LIR_Apic_2	765	771	PF02991	0.546
LIG_LIR_Gen_1	19	26	PF02991	0.515
LIG_LIR_Gen_1	284	295	PF02991	0.499
LIG_LIR_Gen_1	30	39	PF02991	0.384
LIG_LIR_Gen_1	344	353	PF02991	0.441
LIG_LIR_Gen_1	460	469	PF02991	0.486
LIG_LIR_Nem_3	19	23	PF02991	0.487
LIG_LIR_Nem_3	25	29	PF02991	0.403
LIG_LIR_Nem_3	284	290	PF02991	0.516
LIG_LIR_Nem_3	294	299	PF02991	0.411
LIG_LIR_Nem_3	30	36	PF02991	0.345
LIG_LIR_Nem_3	344	349	PF02991	0.428
LIG_LIR_Nem_3	460	466	PF02991	0.452
LIG_LIR_Nem_3	55	60	PF02991	0.459
LIG_MYND_3	466	470	PF01753	0.316
LIG_PCNA_PIPBox_1	374	383	PF02747	0.487
LIG_PDZ_Class_2	779	784	PF00595	0.433
LIG_Pex14_1	136	140	PF04695	0.364
LIG_Pex14_1	253	257	PF04695	0.435
LIG_SH2_CRK	287	291	PF00017	0.482
LIG_SH2_CRK	76	80	PF00017	0.404
LIG_SH2_GRB2like	505	508	PF00017	0.320
LIG_SH2_NCK_1	26	30	PF00017	0.312
LIG_SH2_STAP1	210	214	PF00017	0.515
LIG_SH2_STAP1	287	291	PF00017	0.482
LIG_SH2_STAP1	704	708	PF00017	0.586
LIG_SH2_STAT5	133	136	PF00017	0.465
LIG_SH2_STAT5	140	143	PF00017	0.510
LIG_SH2_STAT5	210	213	PF00017	0.445
LIG_SH2_STAT5	499	502	PF00017	0.581
LIG_SH2_STAT5	505	508	PF00017	0.532
LIG_SH2_STAT5	527	530	PF00017	0.425
LIG_SH2_STAT5	673	676	PF00017	0.294
LIG_SH2_STAT5	81	84	PF00017	0.449
LIG_SH3_2	308	313	PF14604	0.539
LIG_SH3_3	120	126	PF00018	0.450
LIG_SH3_3	167	173	PF00018	0.451
LIG_SH3_3	305	311	PF00018	0.481
LIG_SH3_3	555	561	PF00018	0.447
LIG_SH3_3	634	640	PF00018	0.498
LIG_SH3_3	712	718	PF00018	0.659
LIG_SUMO_SIM_anti_2	360	365	PF11976	0.407
LIG_SUMO_SIM_par_1	452	460	PF11976	0.598
LIG_TRAF2_1	4	7	PF00917	0.555
LIG_TRAF2_1	8	11	PF00917	0.557
LIG_TYR_ITIM	74	79	PF00017	0.413
LIG_UBA3_1	320	326	PF00899	0.546
LIG_UBA3_1	680	687	PF00899	0.289
MOD_CDC14_SPxK_1	734	737	PF00782	0.581
MOD_CDK_SPK_2	436	441	PF00069	0.644
MOD_CDK_SPxK_1	731	737	PF00069	0.578
MOD_CDK_SPxxK_3	436	443	PF00069	0.717
MOD_CK1_1	127	133	PF00069	0.485
MOD_CK1_1	169	175	PF00069	0.600
MOD_CK1_1	2	8	PF00069	0.608
MOD_CK1_1	411	417	PF00069	0.554
MOD_CK1_1	423	429	PF00069	0.510
MOD_CK1_1	66	72	PF00069	0.429
MOD_CK1_1	710	716	PF00069	0.620
MOD_CK1_1	731	737	PF00069	0.693
MOD_CK2_1	1	7	PF00069	0.560
MOD_CK2_1	182	188	PF00069	0.778
MOD_CK2_1	341	347	PF00069	0.507
MOD_CK2_1	551	557	PF00069	0.554
MOD_Cter_Amidation	395	398	PF01082	0.412
MOD_GlcNHglycan	184	187	PF01048	0.668
MOD_GlcNHglycan	194	197	PF01048	0.667
MOD_GlcNHglycan	200	203	PF01048	0.641
MOD_GlcNHglycan	283	286	PF01048	0.530
MOD_GlcNHglycan	39	42	PF01048	0.443
MOD_GSK3_1	1	8	PF00069	0.677
MOD_GSK3_1	198	205	PF00069	0.553
MOD_GSK3_1	274	281	PF00069	0.339
MOD_GSK3_1	372	379	PF00069	0.432
MOD_GSK3_1	398	405	PF00069	0.669
MOD_GSK3_1	419	426	PF00069	0.572
MOD_GSK3_1	450	457	PF00069	0.675
MOD_GSK3_1	557	564	PF00069	0.547
MOD_GSK3_1	576	583	PF00069	0.467
MOD_GSK3_1	63	70	PF00069	0.575
MOD_GSK3_1	664	671	PF00069	0.524
MOD_GSK3_1	727	734	PF00069	0.680
MOD_GSK3_1	736	743	PF00069	0.598
MOD_N-GLC_1	175	180	PF02516	0.667
MOD_N-GLC_1	198	203	PF02516	0.411
MOD_N-GLC_1	551	556	PF02516	0.440
MOD_N-GLC_2	675	677	PF02516	0.294
MOD_NEK2_1	166	171	PF00069	0.484
MOD_NEK2_1	190	195	PF00069	0.721
MOD_NEK2_1	198	203	PF00069	0.662
MOD_NEK2_1	269	274	PF00069	0.429
MOD_NEK2_1	278	283	PF00069	0.484
MOD_NEK2_1	489	494	PF00069	0.462
MOD_NEK2_1	551	556	PF00069	0.427
MOD_NEK2_1	728	733	PF00069	0.610
MOD_OFUCOSY	515	520	PF10250	0.271
MOD_PIKK_1	208	214	PF00454	0.370
MOD_PIKK_1	568	574	PF00454	0.418
MOD_PIKK_1	757	763	PF00454	0.502
MOD_PK_1	95	101	PF00069	0.412
MOD_PKA_1	37	43	PF00069	0.443
MOD_PKA_2	235	241	PF00069	0.485
MOD_PKA_2	37	43	PF00069	0.443
MOD_PKA_2	420	426	PF00069	0.623
MOD_PKA_2	476	482	PF00069	0.474
MOD_PKA_2	664	670	PF00069	0.554
MOD_PKB_1	406	414	PF00069	0.471
MOD_Plk_1	551	557	PF00069	0.503
MOD_Plk_1	576	582	PF00069	0.468
MOD_Plk_4	101	107	PF00069	0.317
MOD_Plk_4	245	251	PF00069	0.323
MOD_Plk_4	362	368	PF00069	0.396
MOD_Plk_4	376	382	PF00069	0.413
MOD_Plk_4	551	557	PF00069	0.501
MOD_Plk_4	668	674	PF00069	0.409
MOD_ProDKin_1	122	128	PF00069	0.442
MOD_ProDKin_1	169	175	PF00069	0.547
MOD_ProDKin_1	355	361	PF00069	0.389
MOD_ProDKin_1	436	442	PF00069	0.633
MOD_ProDKin_1	557	563	PF00069	0.473
MOD_ProDKin_1	729	735	PF00069	0.635
MOD_ProDKin_1	736	742	PF00069	0.630
TRG_DiLeu_BaEn_2	383	389	PF01217	0.430
TRG_DiLeu_BaEn_2	52	58	PF01217	0.255
TRG_DiLeu_BaLyEn_6	640	645	PF01217	0.495
TRG_DiLeu_BaLyEn_6	69	74	PF01217	0.441
TRG_ENDOCYTIC_2	140	143	PF00928	0.375
TRG_ENDOCYTIC_2	26	29	PF00928	0.451
TRG_ENDOCYTIC_2	287	290	PF00928	0.538
TRG_ENDOCYTIC_2	76	79	PF00928	0.372
TRG_ER_diArg_1	158	161	PF00400	0.407
TRG_ER_diArg_1	406	409	PF00400	0.622
TRG_ER_diArg_1	415	417	PF00400	0.550
TRG_ER_diArg_1	442	445	PF00400	0.644
TRG_ER_diArg_1	471	474	PF00400	0.374
TRG_ER_diArg_1	485	487	PF00400	0.521
TRG_ER_diArg_1	540	542	PF00400	0.559
TRG_ER_diArg_1	71	73	PF00400	0.529
TRG_ER_diArg_1	78	81	PF00400	0.447
TRG_NES_CRM1_1	371	384	PF08389	0.493
TRG_NES_CRM1_1	545	557	PF08389	0.452
TRG_Pf-PMV_PEXEL_1	149	154	PF00026	0.412
TRG_Pf-PMV_PEXEL_1	163	168	PF00026	0.510
TRG_Pf-PMV_PEXEL_1	266	271	PF00026	0.455

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IJU9	Leptomonas seymouri	46%	98%
A0A0S4JDR0	Bodo saltans	26%	85%
A0A1X0NY20	Trypanosomatidae	31%	100%
A0A3S7WVR9	Leishmania donovani	74%	98%
A0A422NEI4	Trypanosoma rangeli	30%	100%
A4HYM1	Leishmania infantum	74%	98%
C9ZHT5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	29%	100%
E9AUH0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	73%	98%
Q4QD14	Leishmania major	73%	100%