EF-hand domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Repeat of unknown function (DUF1126), putative

Species:

Leishmania braziliensis

UniProt:

E9AI34_LEIBR

TriTrypDb:

LbrM.08.0890 , LBRM2903_080016700

Length:

859

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 1, no: 29
NetGPI	no	yes: 0, no: 30

Cellular components
Term	Name	Level	Count
GO:0005819	spindle	5	3
GO:0005930	axoneme	2	3
GO:0043226	organelle	2	3
GO:0043228	non-membrane-bounded organelle	3	3
GO:0043229	intracellular organelle	3	3
GO:0043232	intracellular non-membrane-bounded organelle	4	3
GO:0072686	mitotic spindle	6	3
GO:0110165	cellular anatomical entity	1	3

Expansion

Sequence features

E9AI34

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AI34

Function

Biological processes
Term	Name	Level	Count
GO:0000226	microtubule cytoskeleton organization	3	3
GO:0000281	mitotic cytokinesis	4	3
GO:0000910	cytokinesis	3	3
GO:0001539	cilium or flagellum-dependent cell motility	3	3
GO:0006996	organelle organization	4	3
GO:0007010	cytoskeleton organization	5	3
GO:0007017	microtubule-based process	2	3
GO:0007051	spindle organization	3	3
GO:0007052	mitotic spindle organization	4	3
GO:0009987	cellular process	1	3
GO:0016043	cellular component organization	3	3
GO:0022402	cell cycle process	2	3
GO:0048870	cell motility	2	3
GO:0060285	cilium-dependent cell motility	4	3
GO:0061640	cytoskeleton-dependent cytokinesis	4	3
GO:0071840	cellular component organization or biogenesis	2	3
GO:1902850	microtubule cytoskeleton organization involved in mitosis	4	3
GO:1903047	mitotic cell cycle process	3	3

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	20
GO:0005509	calcium ion binding	5	19
GO:0043167	ion binding	2	19
GO:0043169	cation binding	3	19
GO:0046872	metal ion binding	4	19
GO:0005515	protein binding	2	3
GO:0008092	cytoskeletal protein binding	3	3
GO:0015631	tubulin binding	4	3
GO:0043014	alpha-tubulin binding	5	3

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	163	167	PF00656	0.553
CLV_C14_Caspase3-7	290	294	PF00656	0.428
CLV_C14_Caspase3-7	448	452	PF00656	0.756
CLV_C14_Caspase3-7	738	742	PF00656	0.546
CLV_MEL_PAP_1	30	36	PF00089	0.606
CLV_NRD_NRD_1	100	102	PF00675	0.633
CLV_NRD_NRD_1	139	141	PF00675	0.284
CLV_NRD_NRD_1	301	303	PF00675	0.278
CLV_NRD_NRD_1	423	425	PF00675	0.535
CLV_NRD_NRD_1	495	497	PF00675	0.271
CLV_NRD_NRD_1	527	529	PF00675	0.303
CLV_NRD_NRD_1	725	727	PF00675	0.535
CLV_NRD_NRD_1	764	766	PF00675	0.599
CLV_NRD_NRD_1	786	788	PF00675	0.426
CLV_PCSK_FUR_1	58	62	PF00082	0.502
CLV_PCSK_KEX2_1	172	174	PF00082	0.294
CLV_PCSK_KEX2_1	301	303	PF00082	0.309
CLV_PCSK_KEX2_1	423	425	PF00082	0.726
CLV_PCSK_KEX2_1	495	497	PF00082	0.277
CLV_PCSK_KEX2_1	527	529	PF00082	0.297
CLV_PCSK_KEX2_1	60	62	PF00082	0.505
CLV_PCSK_KEX2_1	725	727	PF00082	0.541
CLV_PCSK_KEX2_1	764	766	PF00082	0.602
CLV_PCSK_KEX2_1	786	788	PF00082	0.442
CLV_PCSK_KEX2_1	837	839	PF00082	0.476
CLV_PCSK_PC1ET2_1	172	174	PF00082	0.284
CLV_PCSK_PC1ET2_1	527	529	PF00082	0.295
CLV_PCSK_PC1ET2_1	60	62	PF00082	0.484
CLV_PCSK_PC1ET2_1	837	839	PF00082	0.578
CLV_PCSK_SKI1_1	110	114	PF00082	0.374
CLV_PCSK_SKI1_1	220	224	PF00082	0.421
CLV_PCSK_SKI1_1	385	389	PF00082	0.271
CLV_PCSK_SKI1_1	397	401	PF00082	0.359
CLV_PCSK_SKI1_1	424	428	PF00082	0.719
CLV_PCSK_SKI1_1	481	485	PF00082	0.319
CLV_PCSK_SKI1_1	495	499	PF00082	0.308
CLV_PCSK_SKI1_1	530	534	PF00082	0.306
CLV_PCSK_SKI1_1	60	64	PF00082	0.501
CLV_PCSK_SKI1_1	734	738	PF00082	0.446
DEG_APCC_DBOX_1	219	227	PF00400	0.392
DEG_SPOP_SBC_1	855	859	PF00917	0.538
DOC_ANK_TNKS_1	291	298	PF00023	0.541
DOC_CKS1_1	779	784	PF01111	0.448
DOC_CYCLIN_yCln2_LP_2	29	32	PF00134	0.586
DOC_CYCLIN_yCln2_LP_2	408	414	PF00134	0.504
DOC_CYCLIN_yCln2_LP_2	484	490	PF00134	0.542
DOC_MAPK_DCC_7	541	549	PF00069	0.368
DOC_MAPK_DCC_7	776	785	PF00069	0.425
DOC_MAPK_FxFP_2	808	811	PF00069	0.402
DOC_MAPK_gen_1	140	146	PF00069	0.501
DOC_MAPK_gen_1	274	283	PF00069	0.263
DOC_MAPK_gen_1	301	309	PF00069	0.468
DOC_MAPK_gen_1	527	534	PF00069	0.560
DOC_MAPK_gen_1	642	650	PF00069	0.403
DOC_MAPK_MEF2A_6	277	285	PF00069	0.368
DOC_MAPK_MEF2A_6	301	309	PF00069	0.508
DOC_MAPK_MEF2A_6	370	378	PF00069	0.510
DOC_PP1_RVXF_1	115	122	PF00149	0.428
DOC_PP1_RVXF_1	269	276	PF00149	0.484
DOC_PP2B_LxvP_1	29	32	PF13499	0.593
DOC_PP2B_LxvP_1	408	411	PF13499	0.507
DOC_PP2B_LxvP_1	547	550	PF13499	0.376
DOC_PP4_FxxP_1	125	128	PF00568	0.548
DOC_PP4_FxxP_1	771	774	PF00568	0.668
DOC_PP4_FxxP_1	808	811	PF00568	0.379
DOC_SPAK_OSR1_1	496	500	PF12202	0.528
DOC_USP7_MATH_1	12	16	PF00917	0.654
DOC_USP7_MATH_1	269	273	PF00917	0.317
DOC_USP7_MATH_1	432	436	PF00917	0.745
DOC_USP7_MATH_1	455	459	PF00917	0.719
DOC_USP7_MATH_1	460	464	PF00917	0.766
DOC_USP7_MATH_1	588	592	PF00917	0.602
DOC_USP7_MATH_1	727	731	PF00917	0.582
DOC_USP7_MATH_1	854	858	PF00917	0.644
DOC_USP7_MATH_1	88	92	PF00917	0.616
DOC_USP7_UBL2_3	529	533	PF12436	0.490
DOC_WW_Pin1_4	674	679	PF00397	0.577
DOC_WW_Pin1_4	778	783	PF00397	0.413
DOC_WW_Pin1_4	793	798	PF00397	0.419
LIG_14-3-3_CanoR_1	110	115	PF00244	0.336
LIG_14-3-3_CanoR_1	204	213	PF00244	0.575
LIG_14-3-3_CanoR_1	319	329	PF00244	0.513
LIG_14-3-3_CanoR_1	403	409	PF00244	0.566
LIG_14-3-3_CanoR_1	423	427	PF00244	0.667
LIG_14-3-3_CanoR_1	495	500	PF00244	0.457
LIG_14-3-3_CanoR_1	6	12	PF00244	0.694
LIG_14-3-3_CanoR_1	702	706	PF00244	0.675
LIG_14-3-3_CanoR_1	752	758	PF00244	0.385
LIG_14-3-3_CanoR_1	96	103	PF00244	0.618
LIG_APCC_ABBA_1	305	310	PF00400	0.517
LIG_BIR_II_1	1	5	PF00653	0.661
LIG_BIR_III_4	454	458	PF00653	0.552
LIG_BIR_III_4	607	611	PF00653	0.650
LIG_BRCT_BRCA1_1	192	196	PF00533	0.575
LIG_BRCT_BRCA1_1	253	257	PF00533	0.570
LIG_BRCT_BRCA1_1	271	275	PF00533	0.580
LIG_BRCT_BRCA1_1	504	508	PF00533	0.576
LIG_FHA_1	150	156	PF00498	0.459
LIG_FHA_1	343	349	PF00498	0.410
LIG_FHA_1	377	383	PF00498	0.484
LIG_FHA_1	431	437	PF00498	0.659
LIG_FHA_1	473	479	PF00498	0.383
LIG_FHA_1	701	707	PF00498	0.717
LIG_FHA_1	98	104	PF00498	0.605
LIG_FHA_2	111	117	PF00498	0.381
LIG_FHA_2	501	507	PF00498	0.467
LIG_FHA_2	555	561	PF00498	0.488
LIG_FHA_2	591	597	PF00498	0.634
LIG_FHA_2	684	690	PF00498	0.412
LIG_FHA_2	770	776	PF00498	0.631
LIG_FHA_2	796	802	PF00498	0.409
LIG_GBD_Chelix_1	618	626	PF00786	0.473
LIG_Integrin_RGD_1	765	767	PF01839	0.433
LIG_LIR_Apic_2	770	774	PF02991	0.607
LIG_LIR_Apic_2	807	811	PF02991	0.465
LIG_LIR_Gen_1	189	199	PF02991	0.465
LIG_LIR_Gen_1	636	643	PF02991	0.449
LIG_LIR_Gen_1	682	691	PF02991	0.456
LIG_LIR_Nem_3	145	149	PF02991	0.567
LIG_LIR_Nem_3	189	194	PF02991	0.450
LIG_LIR_Nem_3	208	213	PF02991	0.478
LIG_LIR_Nem_3	243	248	PF02991	0.487
LIG_LIR_Nem_3	254	260	PF02991	0.579
LIG_LIR_Nem_3	366	372	PF02991	0.541
LIG_LIR_Nem_3	477	483	PF02991	0.577
LIG_LIR_Nem_3	636	640	PF02991	0.417
LIG_LIR_Nem_3	682	688	PF02991	0.485
LIG_LIR_Nem_3	807	813	PF02991	0.476
LIG_MYND_1	27	31	PF01753	0.681
LIG_PCNA_PIPBox_1	802	811	PF02747	0.400
LIG_PCNA_yPIPBox_3	80	90	PF02747	0.410
LIG_Pex14_2	121	125	PF04695	0.462
LIG_Pex14_2	218	222	PF04695	0.493
LIG_REV1ctd_RIR_1	397	407	PF16727	0.364
LIG_SH2_PTP2	191	194	PF00017	0.556
LIG_SH2_STAP1	395	399	PF00017	0.519
LIG_SH2_STAT5	191	194	PF00017	0.535
LIG_SH2_STAT5	21	24	PF00017	0.576
LIG_SH2_STAT5	330	333	PF00017	0.528
LIG_SH2_STAT5	395	398	PF00017	0.550
LIG_SH2_STAT5	417	420	PF00017	0.614
LIG_SH2_STAT5	502	505	PF00017	0.480
LIG_SH2_STAT5	556	559	PF00017	0.490
LIG_SH2_STAT5	625	628	PF00017	0.444
LIG_SH2_STAT5	684	687	PF00017	0.445
LIG_SH2_STAT5	818	821	PF00017	0.529
LIG_SH2_STAT5	840	843	PF00017	0.363
LIG_SH3_3	174	180	PF00018	0.561
LIG_SH3_3	227	233	PF00018	0.408
LIG_SH3_3	440	446	PF00018	0.747
LIG_SH3_3	532	538	PF00018	0.555
LIG_SH3_3	645	651	PF00018	0.509
LIG_SH3_3	776	782	PF00018	0.553
LIG_SH3_3	808	814	PF00018	0.446
LIG_SH3_CIN85_PxpxPR_1	536	541	PF14604	0.558
LIG_SUMO_SIM_anti_2	313	318	PF11976	0.427
LIG_SUMO_SIM_anti_2	686	692	PF11976	0.545
LIG_SUMO_SIM_par_1	150	157	PF11976	0.465
LIG_SUMO_SIM_par_1	410	415	PF11976	0.507
LIG_SUMO_SIM_par_1	611	616	PF11976	0.554
LIG_SUMO_SIM_par_1	645	652	PF11976	0.440
LIG_TRAF2_1	105	108	PF00917	0.661
LIG_TRAF2_1	187	190	PF00917	0.506
LIG_TRAF2_2	72	77	PF00917	0.457
LIG_WRC_WIRS_1	143	148	PF05994	0.441
LIG_WRC_WIRS_1	634	639	PF05994	0.457
LIG_WRC_WIRS_1	768	773	PF05994	0.699
LIG_WW_3	30	34	PF00397	0.609
MOD_CDK_SPxK_1	674	680	PF00069	0.308
MOD_CK1_1	322	328	PF00069	0.420
MOD_CK1_1	435	441	PF00069	0.720
MOD_CK1_1	463	469	PF00069	0.610
MOD_CK2_1	318	324	PF00069	0.351
MOD_CK2_1	500	506	PF00069	0.337
MOD_CK2_1	590	596	PF00069	0.561
MOD_CK2_1	646	652	PF00069	0.420
MOD_CK2_1	795	801	PF00069	0.457
MOD_Cter_Amidation	58	61	PF01082	0.487
MOD_Cter_Amidation	762	765	PF01082	0.627
MOD_GlcNHglycan	162	165	PF01048	0.324
MOD_GlcNHglycan	253	256	PF01048	0.512
MOD_GlcNHglycan	447	450	PF01048	0.652
MOD_GlcNHglycan	465	468	PF01048	0.758
MOD_GlcNHglycan	512	515	PF01048	0.332
MOD_GlcNHglycan	590	593	PF01048	0.494
MOD_GlcNHglycan	712	715	PF01048	0.685
MOD_GSK3_1	318	325	PF00069	0.349
MOD_GSK3_1	338	345	PF00069	0.402
MOD_GSK3_1	422	429	PF00069	0.634
MOD_GSK3_1	588	595	PF00069	0.497
MOD_GSK3_1	617	624	PF00069	0.446
MOD_GSK3_1	625	632	PF00069	0.369
MOD_GSK3_1	63	70	PF00069	0.711
MOD_GSK3_1	679	686	PF00069	0.461
MOD_GSK3_1	701	708	PF00069	0.641
MOD_GSK3_1	791	798	PF00069	0.551
MOD_N-GLC_1	12	17	PF02516	0.630
MOD_N-GLC_1	205	210	PF02516	0.259
MOD_N-GLC_1	216	221	PF02516	0.199
MOD_N-GLC_1	67	72	PF02516	0.654
MOD_N-GLC_1	823	828	PF02516	0.552
MOD_NEK2_1	329	334	PF00069	0.353
MOD_NEK2_1	348	353	PF00069	0.405
MOD_NEK2_1	383	388	PF00069	0.451
MOD_NEK2_1	7	12	PF00069	0.621
MOD_NEK2_1	705	710	PF00069	0.674
MOD_NEK2_1	753	758	PF00069	0.386
MOD_NEK2_1	78	83	PF00069	0.524
MOD_NEK2_1	89	94	PF00069	0.495
MOD_NEK2_2	269	274	PF00069	0.315
MOD_NEK2_2	404	409	PF00069	0.376
MOD_NEK2_2	474	479	PF00069	0.259
MOD_PIKK_1	258	264	PF00454	0.533
MOD_PIKK_1	63	69	PF00454	0.635
MOD_PIKK_1	816	822	PF00454	0.420
MOD_PK_1	338	344	PF00069	0.400
MOD_PKA_1	495	501	PF00069	0.369
MOD_PKA_2	291	297	PF00069	0.369
MOD_PKA_2	318	324	PF00069	0.344
MOD_PKA_2	422	428	PF00069	0.676
MOD_PKA_2	495	501	PF00069	0.348
MOD_PKA_2	588	594	PF00069	0.568
MOD_PKA_2	679	685	PF00069	0.595
MOD_PKA_2	7	13	PF00069	0.676
MOD_PKA_2	701	707	PF00069	0.644
MOD_PKA_2	775	781	PF00069	0.507
MOD_PKA_2	95	101	PF00069	0.682
MOD_Plk_1	149	155	PF00069	0.331
MOD_Plk_1	269	275	PF00069	0.514
MOD_Plk_1	310	316	PF00069	0.317
MOD_Plk_1	346	352	PF00069	0.346
MOD_Plk_1	651	657	PF00069	0.535
MOD_Plk_1	67	73	PF00069	0.700
MOD_Plk_2-3	695	701	PF00069	0.552
MOD_Plk_4	133	139	PF00069	0.391
MOD_Plk_4	149	155	PF00069	0.205
MOD_Plk_4	582	588	PF00069	0.560
MOD_Plk_4	633	639	PF00069	0.497
MOD_Plk_4	701	707	PF00069	0.739
MOD_Plk_4	775	781	PF00069	0.517
MOD_Plk_4	843	849	PF00069	0.636
MOD_ProDKin_1	674	680	PF00069	0.587
MOD_ProDKin_1	778	784	PF00069	0.411
MOD_ProDKin_1	793	799	PF00069	0.420
MOD_SUMO_rev_2	559	567	PF00179	0.439
MOD_SUMO_rev_2	713	723	PF00179	0.585
MOD_SUMO_rev_2	730	738	PF00179	0.547
TRG_DiLeu_BaEn_1	563	568	PF01217	0.175
TRG_DiLeu_BaEn_2	303	309	PF01217	0.344
TRG_DiLeu_BaEn_4	189	195	PF01217	0.441
TRG_ENDOCYTIC_2	122	125	PF00928	0.360
TRG_ENDOCYTIC_2	191	194	PF00928	0.422
TRG_ENDOCYTIC_2	286	289	PF00928	0.391
TRG_ENDOCYTIC_2	369	372	PF00928	0.441
TRG_ENDOCYTIC_2	839	842	PF00928	0.460
TRG_ER_diArg_1	180	183	PF00400	0.341
TRG_ER_diArg_1	300	302	PF00400	0.392
TRG_ER_diArg_1	495	497	PF00400	0.341
TRG_ER_diArg_1	5	8	PF00400	0.695
TRG_ER_diArg_1	785	787	PF00400	0.412
TRG_NLS_MonoCore_2	526	531	PF00514	0.441
TRG_NLS_MonoExtN_4	527	532	PF00514	0.353
TRG_Pf-PMV_PEXEL_1	274	278	PF00026	0.468
TRG_Pf-PMV_PEXEL_1	731	735	PF00026	0.473
TRG_Pf-PMV_PEXEL_1	787	791	PF00026	0.414

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P5H4	Leptomonas seymouri	69%	100%
A0A0N1HZ98	Leptomonas seymouri	23%	100%
A0A0N1I3M2	Leptomonas seymouri	21%	100%
A0A0S4IJW8	Bodo saltans	35%	100%
A0A0S4IQM0	Bodo saltans	25%	100%
A0A0S4J8J7	Bodo saltans	24%	94%
A0A1X0NEV8	Trypanosomatidae	46%	100%
A0A1X0P419	Trypanosomatidae	23%	100%
A0A3Q8IDH4	Leishmania donovani	24%	100%
A0A3Q8IPU3	Leishmania donovani	23%	100%
A0A3R7KM50	Trypanosoma rangeli	48%	100%
A0A3R7L048	Trypanosoma rangeli	23%	100%
A0A3S5H688	Leishmania donovani	87%	99%
A0A422NK00	Trypanosoma rangeli	27%	100%
A4HE78	Leishmania braziliensis	24%	100%
A4HFK9	Leishmania braziliensis	23%	100%
A4HTP5	Leishmania infantum	87%	99%
A4I1J2	Leishmania infantum	24%	100%
C9ZJX4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	23%	100%
C9ZPE7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	100%
E9AD64	Leishmania major	23%	100%
E9AHF6	Leishmania infantum	23%	100%
E9AMI2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	100%
E9AXM7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	100%
E9AYY9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	23%	100%
Q4Q9U5	Leishmania major	24%	99%
Q4QI57	Leishmania major	87%	100%
V5ASV2	Trypanosoma cruzi	24%	100%
V5D3X9	Trypanosoma cruzi	23%	100%