LeishMANIAdb
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Amino acid permease

Quick info Annotations Function or PPIs Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:
Amino acid permease
Gene product:
amino acid permease
Species:
Leishmania infantum
UniProt:
E9AHL1_LEIIN
TriTrypDb:
LINF_310025550
Length:
605

Annotations

LeishMANIAdb annotations

An enormously expanded group of various amino acid transporters.. Localization: Cell surface (by feature)

Annotations by Jardim et al.

Transporters, Amino acid permease AAT8.1

Localization

Secreted promastigote
Source Evidence on protein Close homologs
Cuervo et al. no yes: 0
Hassani et al. no yes: 0
Forrest at al. (metacyclic) no yes: 8
Forrest at al. (procyclic) no yes: 8
Silverman et al. no yes: 0
Pissara et al. no yes: 51
Secreted amastigote
Source Evidence on protein Close homologs
Pires et al. no yes: 0
Exosome
Source Evidence on protein Close homologs
Silverman et al. no yes: 0
Glycosome
Source Evidence on protein Close homologs
Jamdhade et al. no yes: 0
Predictions
Source Evidence on protein Close homologs
DeepLoc
SignalP6 no yes: 1, no: 87
NetGPI no yes: 0, no: 88
Cellular components
Term Name Level Count
GO:0016020 membrane 2 75
GO:0110165 cellular anatomical entity 1 75
GO:0005634 nucleus 5 1
GO:0005737 cytoplasm 2 1
GO:0005886 plasma membrane 3 1
GO:0020016 ciliary pocket 2 1
GO:0020023 kinetoplast 2 1
GO:0043226 organelle 2 1
GO:0043227 membrane-bounded organelle 3 1
GO:0043229 intracellular organelle 3 1
GO:0043231 intracellular membrane-bounded organelle 4 1

Expansion

Sequence features

E9AHL1
Sequence
MSA
Disorder
Secondary
Topology
Domains
SignalP
GPI
Phosphorylations
ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AHL1

Function

Biological processes
Term Name Level Count
GO:0003333 amino acid transmembrane transport 5 11
GO:0006810 transport 3 11
GO:0006865 amino acid transport 5 11
GO:0009987 cellular process 1 11
GO:0015849 organic acid transport 5 11
GO:0051179 localization 1 11
GO:0051234 establishment of localization 2 11
GO:0055085 transmembrane transport 2 11
GO:0071702 organic substance transport 4 11
GO:0071705 nitrogen compound transport 4 11
GO:1903825 organic acid transmembrane transport 3 11
GO:1905039 carboxylic acid transmembrane transport 4 11
GO:0006811 monoatomic ion transport 4 1
GO:0006812 monoatomic cation transport 5 1
GO:0006820 monoatomic anion transport 5 1
GO:0015711 organic anion transport 5 1
GO:0015802 basic amino acid transport 6 1
GO:0015819 lysine transport 6 1
GO:0046942 carboxylic acid transport 6 1
Molecular functions
Term Name Level Count
GO:0003824 catalytic activity 1 4
GO:0016787 hydrolase activity 2 4
GO:0005215 transporter activity 1 11
GO:0005342 organic acid transmembrane transporter activity 3 11
GO:0015171 amino acid transmembrane transporter activity 5 11
GO:0015179 L-amino acid transmembrane transporter activity 6 11
GO:0022857 transmembrane transporter activity 2 11
GO:0046943 carboxylic acid transmembrane transporter activity 4 11
GO:0008324 monoatomic cation transmembrane transporter activity 4 1
GO:0008509 monoatomic anion transmembrane transporter activity 4 1
GO:0008514 organic anion transmembrane transporter activity 5 1
GO:0015075 monoatomic ion transmembrane transporter activity 3 1
GO:0015174 basic amino acid transmembrane transporter activity 6 1
GO:0015189 L-lysine transmembrane transporter activity 5 1

Putative motif mimicry

Leishmania From To Domain/Motif Score
CLV_C14_Caspase3-7 464 468 PF00656 0.390
CLV_C14_Caspase3-7 498 502 PF00656 0.526
CLV_C14_Caspase3-7 508 512 PF00656 0.456
CLV_NRD_NRD_1 160 162 PF00675 0.308
CLV_NRD_NRD_1 60 62 PF00675 0.475
CLV_PCSK_KEX2_1 160 162 PF00082 0.302
CLV_PCSK_KEX2_1 60 62 PF00082 0.500
CLV_PCSK_PC7_1 56 62 PF00082 0.533
CLV_PCSK_SKI1_1 154 158 PF00082 0.319
CLV_PCSK_SKI1_1 161 165 PF00082 0.310
CLV_PCSK_SKI1_1 218 222 PF00082 0.308
CLV_PCSK_SKI1_1 273 277 PF00082 0.490
CLV_PCSK_SKI1_1 333 337 PF00082 0.304
CLV_PCSK_SKI1_1 36 40 PF00082 0.388
CLV_PCSK_SKI1_1 364 368 PF00082 0.542
CLV_PCSK_SKI1_1 369 373 PF00082 0.552
DEG_APCC_DBOX_1 405 413 PF00400 0.519
DEG_SPOP_SBC_1 25 29 PF00917 0.607
DOC_AGCK_PIF_2 356 361 PF00069 0.379
DOC_MAPK_FxFP_2 556 559 PF00069 0.393
DOC_MAPK_gen_1 60 67 PF00069 0.660
DOC_MAPK_MEF2A_6 218 226 PF00069 0.322
DOC_MAPK_MEF2A_6 242 251 PF00069 0.483
DOC_PP1_RVXF_1 204 211 PF00149 0.205
DOC_PP2B_LxvP_1 105 108 PF13499 0.329
DOC_PP2B_LxvP_1 232 235 PF13499 0.282
DOC_PP2B_LxvP_1 534 537 PF13499 0.307
DOC_PP4_FxxP_1 556 559 PF00568 0.333
DOC_USP7_MATH_1 110 114 PF00917 0.314
DOC_USP7_MATH_1 138 142 PF00917 0.537
DOC_USP7_MATH_1 25 29 PF00917 0.717
DOC_USP7_MATH_1 269 273 PF00917 0.344
DOC_USP7_MATH_1 405 409 PF00917 0.558
DOC_USP7_MATH_1 457 461 PF00917 0.522
DOC_USP7_MATH_1 526 530 PF00917 0.244
DOC_USP7_MATH_1 59 63 PF00917 0.559
DOC_USP7_MATH_1 96 100 PF00917 0.346
LIG_14-3-3_CanoR_1 145 150 PF00244 0.561
LIG_14-3-3_CanoR_1 161 167 PF00244 0.411
LIG_14-3-3_CanoR_1 218 223 PF00244 0.296
LIG_14-3-3_CanoR_1 242 251 PF00244 0.473
LIG_14-3-3_CanoR_1 26 31 PF00244 0.749
LIG_14-3-3_CanoR_1 296 302 PF00244 0.365
LIG_14-3-3_CanoR_1 330 336 PF00244 0.490
LIG_14-3-3_CanoR_1 406 410 PF00244 0.507
LIG_14-3-3_CanoR_1 53 59 PF00244 0.754
LIG_Actin_WH2_2 227 244 PF00022 0.300
LIG_APCC_ABBA_1 375 380 PF00400 0.179
LIG_BIR_II_1 1 5 PF00653 0.591
LIG_BRCT_BRCA1_1 549 553 PF00533 0.348
LIG_BRCT_BRCA1_1 565 569 PF00533 0.480
LIG_BRCT_BRCA1_1 89 93 PF00533 0.304
LIG_EH_1 375 379 PF12763 0.178
LIG_eIF4E_1 579 585 PF01652 0.408
LIG_FHA_1 219 225 PF00498 0.315
LIG_FHA_1 248 254 PF00498 0.344
LIG_FHA_1 27 33 PF00498 0.687
LIG_FHA_1 289 295 PF00498 0.296
LIG_FHA_1 346 352 PF00498 0.402
LIG_FHA_1 505 511 PF00498 0.521
LIG_FHA_1 580 586 PF00498 0.317
LIG_FHA_1 598 604 PF00498 0.341
LIG_FHA_2 482 488 PF00498 0.601
LIG_FHA_2 501 507 PF00498 0.484
LIG_KLC1_Yacidic_2 506 511 PF13176 0.537
LIG_LIR_Gen_1 127 137 PF02991 0.337
LIG_LIR_Gen_1 165 174 PF02991 0.387
LIG_LIR_Gen_1 348 358 PF02991 0.407
LIG_LIR_Gen_1 550 561 PF02991 0.398
LIG_LIR_Gen_1 582 591 PF02991 0.313
LIG_LIR_Gen_1 600 605 PF02991 0.400
LIG_LIR_Nem_3 127 132 PF02991 0.333
LIG_LIR_Nem_3 148 152 PF02991 0.487
LIG_LIR_Nem_3 165 170 PF02991 0.327
LIG_LIR_Nem_3 250 254 PF02991 0.318
LIG_LIR_Nem_3 348 353 PF02991 0.338
LIG_LIR_Nem_3 354 359 PF02991 0.354
LIG_LIR_Nem_3 408 413 PF02991 0.473
LIG_LIR_Nem_3 550 556 PF02991 0.345
LIG_LIR_Nem_3 582 586 PF02991 0.332
LIG_LIR_Nem_3 600 605 PF02991 0.439
LIG_PALB2_WD40_1 572 580 PF16756 0.560
LIG_PDZ_Class_2 600 605 PF00595 0.516
LIG_Pex14_2 173 177 PF04695 0.321
LIG_Pex14_2 251 255 PF04695 0.321
LIG_Pex14_2 558 562 PF04695 0.377
LIG_PTB_Apo_2 509 516 PF02174 0.538
LIG_SH2_CRK 398 402 PF00017 0.297
LIG_SH2_GRB2like 579 582 PF00017 0.426
LIG_SH2_PTP2 350 353 PF00017 0.321
LIG_SH2_PTP2 387 390 PF00017 0.316
LIG_SH2_PTP2 602 605 PF00017 0.427
LIG_SH2_PTP2 80 83 PF00017 0.456
LIG_SH2_SRC 183 186 PF00017 0.294
LIG_SH2_SRC 411 414 PF00017 0.514
LIG_SH2_SRC 499 502 PF00017 0.376
LIG_SH2_STAP1 162 166 PF00017 0.526
LIG_SH2_STAP1 243 247 PF00017 0.312
LIG_SH2_STAT3 312 315 PF00017 0.340
LIG_SH2_STAT5 128 131 PF00017 0.332
LIG_SH2_STAT5 135 138 PF00017 0.340
LIG_SH2_STAT5 183 186 PF00017 0.339
LIG_SH2_STAT5 254 257 PF00017 0.346
LIG_SH2_STAT5 312 315 PF00017 0.260
LIG_SH2_STAT5 350 353 PF00017 0.349
LIG_SH2_STAT5 358 361 PF00017 0.359
LIG_SH2_STAT5 387 390 PF00017 0.345
LIG_SH2_STAT5 411 414 PF00017 0.485
LIG_SH2_STAT5 499 502 PF00017 0.545
LIG_SH2_STAT5 509 512 PF00017 0.496
LIG_SH2_STAT5 579 582 PF00017 0.291
LIG_SH2_STAT5 583 586 PF00017 0.309
LIG_SH2_STAT5 602 605 PF00017 0.353
LIG_SH2_STAT5 80 83 PF00017 0.478
LIG_SH3_3 225 231 PF00018 0.435
LIG_SH3_3 78 84 PF00018 0.531
LIG_Sin3_3 397 404 PF02671 0.408
LIG_SUMO_SIM_anti_2 370 375 PF11976 0.246
LIG_SUMO_SIM_anti_2 582 588 PF11976 0.285
LIG_SUMO_SIM_par_1 135 142 PF11976 0.347
LIG_SUMO_SIM_par_1 523 529 PF11976 0.331
LIG_TRAF2_1 377 380 PF00917 0.346
LIG_TRAF2_2 481 486 PF00917 0.379
LIG_TYR_ITIM 126 131 PF00017 0.329
LIG_TYR_ITIM 385 390 PF00017 0.328
LIG_TYR_ITIM 396 401 PF00017 0.319
LIG_TYR_ITSM 346 353 PF00017 0.362
LIG_TYR_ITSM 598 605 PF00017 0.367
LIG_UBA3_1 229 236 PF00899 0.320
LIG_WRC_WIRS_1 146 151 PF05994 0.494
MOD_CK1_1 130 136 PF00069 0.394
MOD_CK1_1 141 147 PF00069 0.527
MOD_CK1_1 2 8 PF00069 0.679
MOD_CK1_1 28 34 PF00069 0.707
MOD_CK2_1 481 487 PF00069 0.598
MOD_GlcNHglycan 112 115 PF01048 0.465
MOD_GlcNHglycan 244 247 PF01048 0.345
MOD_GlcNHglycan 277 280 PF01048 0.499
MOD_GlcNHglycan 337 340 PF01048 0.333
MOD_GlcNHglycan 4 7 PF01048 0.604
MOD_GlcNHglycan 45 48 PF01048 0.620
MOD_GlcNHglycan 549 552 PF01048 0.366
MOD_GlcNHglycan 565 568 PF01048 0.268
MOD_GlcNHglycan 61 64 PF01048 0.374
MOD_GlcNHglycan 89 92 PF01048 0.319
MOD_GSK3_1 141 148 PF00069 0.474
MOD_GSK3_1 24 31 PF00069 0.687
MOD_GSK3_1 297 304 PF00069 0.365
MOD_GSK3_1 329 336 PF00069 0.508
MOD_GSK3_1 34 41 PF00069 0.654
MOD_GSK3_1 438 445 PF00069 0.597
MOD_GSK3_1 48 55 PF00069 0.693
MOD_GSK3_1 5 12 PF00069 0.644
MOD_GSK3_1 500 507 PF00069 0.537
MOD_GSK3_1 563 570 PF00069 0.494
MOD_GSK3_1 575 582 PF00069 0.499
MOD_GSK3_1 597 604 PF00069 0.294
MOD_LATS_1 24 30 PF00433 0.601
MOD_N-GLC_1 2 7 PF02516 0.461
MOD_N-GLC_1 420 425 PF02516 0.190
MOD_N-GLC_1 567 572 PF02516 0.274
MOD_NEK2_1 210 215 PF00069 0.276
MOD_NEK2_1 268 273 PF00069 0.357
MOD_NEK2_1 294 299 PF00069 0.279
MOD_NEK2_1 335 340 PF00069 0.507
MOD_NEK2_1 345 350 PF00069 0.372
MOD_NEK2_1 367 372 PF00069 0.337
MOD_NEK2_1 472 477 PF00069 0.384
MOD_NEK2_1 95 100 PF00069 0.323
MOD_NEK2_2 405 410 PF00069 0.542
MOD_NEK2_2 457 462 PF00069 0.374
MOD_PIKK_1 211 217 PF00454 0.363
MOD_PIKK_1 41 47 PF00454 0.751
MOD_PIKK_1 479 485 PF00454 0.389
MOD_PIKK_1 48 54 PF00454 0.786
MOD_PKA_2 25 31 PF00069 0.811
MOD_PKA_2 329 335 PF00069 0.457
MOD_PKA_2 405 411 PF00069 0.540
MOD_PKA_2 52 58 PF00069 0.781
MOD_PKA_2 59 65 PF00069 0.683
MOD_PKB_1 418 426 PF00069 0.531
MOD_Plk_1 141 147 PF00069 0.546
MOD_Plk_1 438 444 PF00069 0.384
MOD_Plk_1 504 510 PF00069 0.540
MOD_Plk_1 567 573 PF00069 0.451
MOD_Plk_2-3 505 511 PF00069 0.550
MOD_Plk_4 218 224 PF00069 0.304
MOD_Plk_4 247 253 PF00069 0.325
MOD_Plk_4 288 294 PF00069 0.324
MOD_Plk_4 297 303 PF00069 0.313
MOD_Plk_4 345 351 PF00069 0.308
MOD_Plk_4 367 373 PF00069 0.323
MOD_Plk_4 405 411 PF00069 0.507
MOD_Plk_4 505 511 PF00069 0.528
MOD_Plk_4 526 532 PF00069 0.328
MOD_Plk_4 538 544 PF00069 0.267
MOD_Plk_4 579 585 PF00069 0.338
MOD_Plk_4 597 603 PF00069 0.260
MOD_SUMO_rev_2 278 287 PF00179 0.360
TRG_DiLeu_BaEn_2 428 434 PF01217 0.390
TRG_DiLeu_BaEn_3 379 385 PF01217 0.341
TRG_ENDOCYTIC_2 128 131 PF00928 0.334
TRG_ENDOCYTIC_2 174 177 PF00928 0.400
TRG_ENDOCYTIC_2 254 257 PF00928 0.342
TRG_ENDOCYTIC_2 350 353 PF00928 0.323
TRG_ENDOCYTIC_2 358 361 PF00928 0.317
TRG_ENDOCYTIC_2 387 390 PF00928 0.333
TRG_ENDOCYTIC_2 398 401 PF00928 0.322
TRG_ENDOCYTIC_2 410 413 PF00928 0.473
TRG_ENDOCYTIC_2 583 586 PF00928 0.309
TRG_ENDOCYTIC_2 602 605 PF00928 0.393
TRG_ENDOCYTIC_2 80 83 PF00928 0.589
TRG_ER_diArg_1 160 162 PF00400 0.403
TRG_ER_diArg_1 419 422 PF00400 0.540
TRG_ER_diArg_1 59 61 PF00400 0.723

Homologs

Protein Taxonomy Sequence identity Coverage
A0A0N1IJ81 Leptomonas seymouri 20% 100%
A0A0N1PA98 Leptomonas seymouri 65% 99%
A0A0N1PBP2 Leptomonas seymouri 34% 100%
A0A0S4IV32 Bodo saltans 23% 100%
A0A0S4JU02 Bodo saltans 33% 100%
A0A1X0NR26 Trypanosomatidae 31% 100%
A0A1X0NRL7 Trypanosomatidae 41% 100%
A0A3Q8ICL1 Leishmania donovani 97% 100%
A0A3Q8IDG8 Leishmania donovani 35% 100%
A0A3Q8IG00 Leishmania donovani 40% 100%
A0A3Q8IGE6 Leishmania donovani 92% 100%
A0A3Q8IHG8 Leishmania donovani 32% 100%
A0A3Q8IHH7 Leishmania donovani 20% 100%
A0A3Q8IJH2 Leishmania donovani 31% 100%
A0A3Q8IJX8 Leishmania donovani 30% 100%
A0A3Q8ILE0 Leishmania donovani 34% 100%
A0A3Q8IQG8 Leishmania donovani 25% 100%
A0A3R7JSA0 Trypanosoma rangeli 43% 100%
A0A3R7MWF1 Trypanosoma rangeli 22% 100%
A0A3R7N1H9 Trypanosoma rangeli 44% 100%
A0A3S7WQ39 Leishmania donovani 37% 100%
A0A3S7X443 Leishmania donovani 36% 100%
A0A3S7X458 Leishmania donovani 33% 100%
A0A3S7X4A1 Leishmania donovani 30% 100%
A0A3S7X4L2 Leishmania donovani 98% 100%
A0A3S7X4N4 Leishmania donovani 98% 100%
A0A3S7X4P0 Leishmania donovani 99% 100%
A0A3S7XA86 Leishmania donovani 23% 100%
A0A3S7XCE3 Leishmania donovani 23% 100%
A0A422MW74 Trypanosoma rangeli 37% 100%
A0A422N608 Trypanosoma rangeli 44% 100%
A0A422NZS1 Trypanosoma rangeli 33% 100%
A4H565 Leishmania braziliensis 36% 100%
A4HC75 Leishmania braziliensis 35% 100%
A4HFT5 Leishmania braziliensis 36% 100%
A4HJ07 Leishmania braziliensis 36% 100%
A4HJ09 Leishmania braziliensis 36% 100%
A4HJ35 Leishmania braziliensis 39% 100%
A4HJ63 Leishmania braziliensis 36% 100%
A4HJF8 Leishmania braziliensis 74% 99%
A4HJF9 Leishmania braziliensis 72% 96%
A4HJG1 Leishmania braziliensis 72% 96%
A4HJG3 Leishmania braziliensis 74% 99%
A4HJG5 Leishmania braziliensis 74% 99%
A4HKQ7 Leishmania braziliensis 34% 100%
A4HNJ6 Leishmania braziliensis 24% 100%
A4HNJ7 Leishmania braziliensis 22% 100%
A4HNQ0 Leishmania braziliensis 25% 100%
A4HTE2 Leishmania infantum 37% 100%
A4HZQ0 Leishmania infantum 38% 100%
A4I6I1 Leishmania infantum 33% 100%
A4I6J9 Leishmania infantum 36% 100%
A4I6L3 Leishmania infantum 40% 100%
A4I6L4 Leishmania infantum 33% 100%
A4I6W4 Leishmania infantum 99% 100%
A4I887 Leishmania infantum 32% 100%
A4I914 Leishmania infantum 20% 95%
A4IC53 Leishmania infantum 23% 100%
A4IC66 Leishmania infantum 22% 100%
A4ICC3 Leishmania infantum 23% 100%
A4ICU5 Leishmania infantum 25% 100%
C9ZWR1 Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972) 37% 100%
C9ZWR7 Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972) 27% 100%
D0AAF6 Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972) 31% 100%
D0AAF8 Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972) 31% 100%
E8NHF9 Leishmania mexicana (strain MHOM/GT/2001/U1103) 35% 100%
E8NHS7 Leishmania mexicana (strain MHOM/GT/2001/U1103) 85% 99%
E9ADD7 Leishmania major 33% 100%
E9AG08 Leishmania major 22% 100%
E9AG09 Leishmania major 23% 100%
E9AHD7 Leishmania infantum 33% 100%
E9AHL2 Leishmania infantum 91% 100%
E9AHL3 Leishmania infantum 98% 100%
E9ALD5 Leishmania mexicana (strain MHOM/GT/2001/U1103) 38% 100%
E9ASF9 Leishmania mexicana (strain MHOM/GT/2001/U1103) 24% 100%
E9AUB8 Leishmania mexicana (strain MHOM/GT/2001/U1103) 23% 100%
E9AVK6 Leishmania mexicana (strain MHOM/GT/2001/U1103) 38% 100%
E9AYW9 Leishmania mexicana (strain MHOM/GT/2001/U1103) 32% 100%
E9AZ62 Leishmania mexicana (strain MHOM/GT/2001/U1103) 35% 100%
E9B1I4 Leishmania mexicana (strain MHOM/GT/2001/U1103) 36% 100%
E9B1K7 Leishmania mexicana (strain MHOM/GT/2001/U1103) 40% 100%
E9B1N8 Leishmania mexicana (strain MHOM/GT/2001/U1103) 35% 100%
E9B1N9 Leishmania mexicana (strain MHOM/GT/2001/U1103) 34% 100%
E9B1X9 Leishmania mexicana (strain MHOM/GT/2001/U1103) 85% 100%
E9B1Y0 Leishmania mexicana (strain MHOM/GT/2001/U1103) 86% 99%
E9B345 Leishmania mexicana (strain MHOM/GT/2001/U1103) 33% 100%
E9B3X9 Leishmania mexicana (strain MHOM/GT/2001/U1103) 20% 95%
E9B761 Leishmania mexicana (strain MHOM/GT/2001/U1103) 23% 100%
E9B762 Leishmania mexicana (strain MHOM/GT/2001/U1103) 23% 100%
P40074 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) 23% 100%
Q4Q072 Leishmania major 23% 100%
Q4Q236 Leishmania major 26% 100%
Q4Q509 Leishmania major 33% 100%
Q4Q680 Leishmania major 93% 100%
Q4Q682 Leishmania major 87% 99%
Q4Q683 Leishmania major 92% 100%
Q4Q6H5 Leishmania major 34% 100%
Q4Q6K6 Leishmania major 40% 100%
Q4Q6M9 Leishmania major 35% 100%
Q4QBX3 Leishmania major 38% 100%
Q4QIH0 Leishmania major 37% 100%
Q969I6 Homo sapiens 23% 100%
V5BIJ1 Trypanosoma cruzi 36% 100%
V5D7K9 Trypanosoma cruzi 34% 100%

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LeishMANIAdb - Server version: v0.0.2. - Database version: v0.0.1. - ChangeLog - © 2022-2024 Protein Bioinformatics Research Group, Institute of Enzymology, RCNS