Pyrroline-5-carboxylate reductase

Quick info Annotations Localization Abundance Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

pyrroline-5-carboxylate reductase

Species:

Leishmania infantum

UniProt:

E9AGK4_LEIIN

TriTrypDb:

LINF_130022300

Length:

272

Annotations

Annotations by Jardim et al.

Amino acid metabolism, Pyrroline-5-carboxylate reductase P5CR

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	yes	yes: 3
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 20

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 7

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Could not find GO cellular_component term for this entry.

Abundance

Amastigote (protein)


Source	Evidence on protein	Close homologs
Pescher et al. (upgregulation)	no	yes: 0

Promastigote and amastigote


Source	Evidence on protein	Close homologs
Lahav et al. - mRNA - Protein

Expansion

Sequence features

E9AGK4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AGK4

Function

Biological processes
Term	Name	Level	Count
GO:0006082	organic acid metabolic process	3	11
GO:0006520	amino acid metabolic process	3	11
GO:0006560	proline metabolic process	6	11
GO:0006561	proline biosynthetic process	7	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0008652	amino acid biosynthetic process	4	11
GO:0009058	biosynthetic process	2	11
GO:0009064	glutamine family amino acid metabolic process	5	11
GO:0009084	glutamine family amino acid biosynthetic process	6	11
GO:0009987	cellular process	1	11
GO:0016053	organic acid biosynthetic process	4	11
GO:0018130	heterocycle biosynthetic process	4	11
GO:0019752	carboxylic acid metabolic process	5	11
GO:0043436	oxoacid metabolic process	4	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044249	cellular biosynthetic process	3	11
GO:0044281	small molecule metabolic process	2	11
GO:0044283	small molecule biosynthetic process	3	11
GO:0046394	carboxylic acid biosynthetic process	5	11
GO:0046483	heterocycle metabolic process	3	11
GO:0055129	L-proline biosynthetic process	5	11
GO:0071704	organic substance metabolic process	2	11
GO:1901360	organic cyclic compound metabolic process	3	11
GO:1901362	organic cyclic compound biosynthetic process	4	11
GO:1901564	organonitrogen compound metabolic process	3	11
GO:1901566	organonitrogen compound biosynthetic process	4	11
GO:1901576	organic substance biosynthetic process	3	11
GO:1901605	alpha-amino acid metabolic process	4	11
GO:1901607	alpha-amino acid biosynthetic process	5	11

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0004735	pyrroline-5-carboxylate reductase activity	5	11
GO:0016491	oxidoreductase activity	2	11
GO:0016645	oxidoreductase activity, acting on the CH-NH group of donors	3	11
GO:0016646	oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor	4	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
DOC_CDC14_PxL_1	74	82	PF14671	0.552
DOC_PP1_RVXF_1	3	9	PF00149	0.447
DOC_USP7_MATH_1	61	65	PF00917	0.509
DOC_WW_Pin1_4	172	177	PF00397	0.480
DOC_WW_Pin1_4	218	223	PF00397	0.450
DOC_WW_Pin1_4	228	233	PF00397	0.449
DOC_WW_Pin1_4	85	90	PF00397	0.513
LIG_14-3-3_CanoR_1	197	202	PF00244	0.529
LIG_14-3-3_CanoR_1	38	47	PF00244	0.458
LIG_14-3-3_CanoR_1	53	60	PF00244	0.414
LIG_14-3-3_CterR_2	268	272	PF00244	0.521
LIG_APCC_ABBA_1	179	184	PF00400	0.449
LIG_BIR_II_1	1	5	PF00653	0.550
LIG_BRCT_BRCA1_1	262	266	PF00533	0.552
LIG_FHA_1	100	106	PF00498	0.431
LIG_FHA_1	111	117	PF00498	0.515
LIG_FHA_2	138	144	PF00498	0.364
LIG_FHA_2	233	239	PF00498	0.463
LIG_FHA_2	80	86	PF00498	0.469
LIG_IRF3_LxIS_1	89	95	PF10401	0.400
LIG_LIR_Gen_1	175	186	PF02991	0.464
LIG_LIR_Gen_1	263	270	PF02991	0.623
LIG_MYND_3	77	81	PF01753	0.529
LIG_PCNA_yPIPBox_3	38	50	PF02747	0.531
LIG_Rb_LxCxE_1	254	272	PF01857	0.552
LIG_SH2_CRK	74	78	PF00017	0.552
LIG_SH2_NCK_1	201	205	PF00017	0.480
LIG_SH2_STAP1	201	205	PF00017	0.449
LIG_SH2_STAT5	158	161	PF00017	0.476
LIG_SH2_STAT5	178	181	PF00017	0.439
LIG_SH3_1	117	123	PF00018	0.358
LIG_SH3_3	117	123	PF00018	0.369
LIG_SH3_3	226	232	PF00018	0.438
LIG_Sin3_3	205	212	PF02671	0.480
LIG_UBA3_1	150	156	PF00899	0.410
LIG_UBA3_1	18	26	PF00899	0.480
LIG_UBA3_1	256	264	PF00899	0.529
LIG_UBA3_1	66	72	PF00899	0.493
LIG_WRC_WIRS_1	122	127	PF05994	0.355
MOD_CDK_SPxK_1	85	91	PF00069	0.513
MOD_CK1_1	134	140	PF00069	0.418
MOD_CK1_1	218	224	PF00069	0.464
MOD_CK1_1	39	45	PF00069	0.497
MOD_CK2_1	10	16	PF00069	0.539
MOD_CK2_1	232	238	PF00069	0.463
MOD_CK2_1	248	254	PF00069	0.501
MOD_CK2_1	38	44	PF00069	0.519
MOD_CK2_1	53	59	PF00069	0.519
MOD_CK2_1	79	85	PF00069	0.490
MOD_GlcNHglycan	133	136	PF01048	0.490
MOD_GlcNHglycan	151	154	PF01048	0.370
MOD_GlcNHglycan	162	165	PF01048	0.302
MOD_GlcNHglycan	217	220	PF01048	0.284
MOD_GlcNHglycan	38	41	PF01048	0.297
MOD_GlcNHglycan	55	58	PF01048	0.293
MOD_GSK3_1	228	235	PF00069	0.463
MOD_N-GLC_1	36	41	PF02516	0.249
MOD_N-GLC_2	107	109	PF02516	0.493
MOD_NEK2_1	215	220	PF00069	0.529
MOD_NEK2_1	248	253	PF00069	0.501
MOD_NEK2_1	92	97	PF00069	0.552
MOD_PKA_2	248	254	PF00069	0.501
MOD_Plk_1	30	36	PF00069	0.552
MOD_Plk_4	121	127	PF00069	0.523
MOD_Plk_4	248	254	PF00069	0.487
MOD_Plk_4	30	36	PF00069	0.511
MOD_Plk_4	45	51	PF00069	0.497
MOD_ProDKin_1	172	178	PF00069	0.480
MOD_ProDKin_1	218	224	PF00069	0.450
MOD_ProDKin_1	228	234	PF00069	0.449
MOD_ProDKin_1	85	91	PF00069	0.513
MOD_SUMO_rev_2	2	6	PF00179	0.450
TRG_DiLeu_BaEn_2	237	243	PF01217	0.497
TRG_ENDOCYTIC_2	178	181	PF00928	0.439
TRG_ENDOCYTIC_2	74	77	PF00928	0.515
TRG_Pf-PMV_PEXEL_1	213	217	PF00026	0.309

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I5T1	Leptomonas seymouri	77%	100%
A0A0S4JNV8	Bodo saltans	39%	97%
A0A0S4JPA1	Bodo saltans	33%	99%
A0A0S4JQ86	Bodo saltans	40%	97%
A0A1X0NTV2	Trypanosomatidae	54%	100%
A0A348AXY1	Curvularia clavata	31%	87%
A0A3R7M5B1	Trypanosoma rangeli	55%	100%
A0A3S7WSS3	Leishmania donovani	100%	100%
A0A411KUQ8	Acremonium sp.	24%	86%
A1L2Q8	Xenopus laevis	36%	99%
A4H7F8	Leishmania braziliensis	83%	100%
C9ZRL2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	52%	100%
E0TY11	Bacillus spizizenii (strain ATCC 23059 / NRRL B-14472 / W23)	34%	92%
E9APJ2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	94%	100%
L0E163	Penicillium fellutanum	28%	84%
O04016	Actinidia chinensis var. chinensis	37%	98%
O25773	Helicobacter pylori (strain ATCC 700392 / 26695)	24%	100%
O66553	Aquifex aeolicus (strain VF5)	32%	100%
P0A9L8	Escherichia coli (strain K12)	48%	100%
P0A9L9	Shigella flexneri	48%	100%
P0C1E4	Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)	37%	100%
P0C1E5	Corynebacterium melassecola	37%	100%
P0CI77	Bacillus subtilis (strain 168)	34%	92%
P17817	Glycine max	35%	99%
P22008	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	30%	100%
P22350	Methanobrevibacter smithii	21%	100%
P27771	Treponema pallidum (strain Nichols)	34%	100%
P32263	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	31%	95%
P32322	Homo sapiens	35%	85%
P39696	Bacillus subtilis (strain 168)	25%	100%
P43869	Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)	30%	100%
P46725	Mycobacterium leprae (strain TN)	32%	93%
P52053	Vibrio alginolyticus	32%	100%
P54552	Bacillus subtilis (strain 168)	35%	98%
P54893	Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27)	31%	100%
P54904	Arabidopsis thaliana	36%	99%
P74572	Synechocystis sp. (strain PCC 6803 / Kazusa)	40%	100%
P9WHU6	Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)	32%	92%
P9WHU7	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	32%	92%
Q00777	Bacillus subtilis (strain 168)	23%	100%
Q04708	Pisum sativum	35%	100%
Q12641	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	29%	82%
Q12740	Lophium arboricola	30%	85%
Q17QJ7	Bos taurus	36%	85%
Q20848	Caenorhabditis elegans	34%	91%
Q49XV0	Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41)	29%	100%
Q4L6K3	Staphylococcus haemolyticus (strain JCSC1435)	29%	100%
Q4QFY6	Leishmania major	95%	100%
Q4R531	Macaca fascicularis	31%	99%
Q4R6W7	Macaca fascicularis	40%	85%
Q53H96	Homo sapiens	33%	99%
Q55E34	Dictyostelium discoideum	28%	91%
Q58D08	Bos taurus	31%	88%
Q58DT4	Bos taurus	35%	85%
Q5HFR9	Staphylococcus aureus (strain COL)	25%	100%
Q5HP48	Staphylococcus epidermidis (strain ATCC 35984 / RP62A)	27%	100%
Q5PQJ6	Rattus norvegicus	32%	99%
Q5R9X6	Pongo abelii	35%	85%
Q5RAQ3	Pongo abelii	36%	85%
Q5SPD7	Danio rerio	37%	94%
Q6AY23	Rattus norvegicus	35%	85%
Q6CR99	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	34%	95%
Q6G961	Staphylococcus aureus (strain MSSA476)	25%	100%
Q6GGJ5	Staphylococcus aureus (strain MRSA252)	25%	100%
Q7A0U1	Staphylococcus aureus (strain MW2)	25%	100%
Q7A5G8	Staphylococcus aureus (strain N315)	25%	100%
Q8CP51	Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200)	27%	100%
Q922Q4	Mus musculus	35%	85%
Q922W5	Mus musculus	36%	88%
Q96C36	Homo sapiens	36%	85%
Q99TZ0	Staphylococcus aureus (strain Mu50 / ATCC 700699)	25%	100%
Q9CPE8	Pasteurella multocida (strain Pm70)	31%	99%
Q9DCC4	Mus musculus	33%	99%
Q9HH99	Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)	43%	100%
Q9P7Y7	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	30%	96%
Q9ZK56	Helicobacter pylori (strain J99 / ATCC 700824)	25%	100%
S0DLN5	Gibberella fujikuroi (strain CBS 195.34 / IMI 58289 / NRRL A-6831)	27%	92%
V5BVR2	Trypanosoma cruzi	54%	100%