Surface antigen protein 2

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein - conserved

Species:

Leishmania infantum

UniProt:

E9AGH0_LEIIN

TriTrypDb:

LINF_120012800

Length:

377

Annotations

LeishMANIAdb annotations

Publication identifier(s): 8702946
A surface coat protein involved in immune evasion in Leishmaniids. Extremely fast evolving, almost completely disordered mucin-like protein. . Localization: Cell surface (experimental)

LeishMANIAdb interaction annotations

by homology
Contact email: handman@wehi.edu.au
Publication title: A Leucine-Rich Repeat Motif of Leishmania Parasite Surface Antigen 2 Binds to Macrophages through the Complement Receptor 3
Publication 1st author(s): Kedzierski
Publication Identifier(s): 15067069
Host organism: -1
Interaction detection method(s): fluorescence activated cell sorting
Interaction type: physical association
Identification method participant A: identification by antibody
Identification method participant B: identification by antibody
ID(s) interactor A: P11215
ID(s) interactor B: E9AGG4
Taxid interactor A: Homo sapiens
Taxid interactor B: Leishmania infantum
Biological role(s) interactor A: unspecified role
Biological role(s) interactor B: unspecified role
Experimental role(s) interactor A: unspecified role

Annotations by Jardim et al.

Plasma membrane, Surface antigen 2 LINJ.12.0690

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 150

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 16

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	yes	yes: 59, no: 9
NetGPI	no	yes: 0, no: 68

Cellular components
Term	Name	Level	Count
GO:0005929	cilium	4	69
GO:0042995	cell projection	2	69
GO:0043226	organelle	2	69
GO:0043227	membrane-bounded organelle	3	69
GO:0110165	cellular anatomical entity	1	69
GO:0120025	plasma membrane bounded cell projection	3	69
GO:0016020	membrane	2	24
GO:0005886	plasma membrane	3	5

Expansion

Sequence features

E9AGH0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AGH0

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	3
GO:0004672	protein kinase activity	3	3
GO:0004674	protein serine/threonine kinase activity	4	3
GO:0016301	kinase activity	4	3
GO:0016740	transferase activity	2	3
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	3
GO:0016773	phosphotransferase activity, alcohol group as acceptor	4	3
GO:0140096	catalytic activity, acting on a protein	2	3

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	6	8	PF00675	0.713
CLV_PCSK_KEX2_1	6	8	PF00082	0.713
CLV_PCSK_SKI1_1	255	259	PF00082	0.543
CLV_PCSK_SKI1_1	31	35	PF00082	0.605
CLV_PCSK_SKI1_1	7	11	PF00082	0.689
DEG_APCC_KENBOX_2	33	37	PF00400	0.346
DEG_SCF_FBW7_2	305	310	PF00400	0.402
DOC_AGCK_PIF_2	69	74	PF00069	0.263
DOC_CYCLIN_RxL_1	3	13	PF00134	0.474
DOC_MAPK_MEF2A_6	277	284	PF00069	0.348
DOC_USP7_MATH_1	192	196	PF00917	0.411
DOC_USP7_MATH_1	216	220	PF00917	0.262
DOC_USP7_MATH_1	257	261	PF00917	0.441
DOC_USP7_MATH_1	353	357	PF00917	0.470
DOC_USP7_MATH_1	41	45	PF00917	0.478
DOC_WW_Pin1_4	301	306	PF00397	0.406
LIG_14-3-3_CanoR_1	43	48	PF00244	0.445
LIG_BIR_II_1	1	5	PF00653	0.603
LIG_FHA_1	103	109	PF00498	0.314
LIG_FHA_1	131	137	PF00498	0.337
LIG_FHA_1	145	151	PF00498	0.351
LIG_FHA_1	341	347	PF00498	0.377
LIG_FHA_1	44	50	PF00498	0.423
LIG_FHA_2	108	114	PF00498	0.331
LIG_FHA_2	183	189	PF00498	0.355
LIG_FHA_2	207	213	PF00498	0.346
LIG_FHA_2	309	315	PF00498	0.494
LIG_FHA_2	37	43	PF00498	0.292
LIG_FHA_2	92	98	PF00498	0.404
LIG_LIR_Gen_1	101	112	PF02991	0.439
LIG_LIR_Gen_1	113	121	PF02991	0.388
LIG_LIR_Gen_1	128	136	PF02991	0.316
LIG_LIR_Gen_1	153	160	PF02991	0.356
LIG_LIR_Gen_1	248	257	PF02991	0.405
LIG_LIR_Nem_3	101	107	PF02991	0.427
LIG_PCNA_PIPBox_1	132	141	PF02747	0.245
LIG_PDZ_Class_2	372	377	PF00595	0.282
LIG_SH2_CRK	104	108	PF00017	0.459
LIG_SH2_GRB2like	104	107	PF00017	0.458
LIG_SH2_STAP1	104	108	PF00017	0.460
LIG_SH2_STAP1	78	82	PF00017	0.296
LIG_SH2_STAT5	104	107	PF00017	0.364
LIG_SH2_STAT5	336	339	PF00017	0.467
LIG_SH2_STAT5	347	350	PF00017	0.341
LIG_SH2_STAT5	74	77	PF00017	0.367
LIG_SH3_3	93	99	PF00018	0.428
LIG_SUMO_SIM_anti_2	182	188	PF11976	0.264
LIG_SUMO_SIM_anti_2	206	212	PF11976	0.254
LIG_SUMO_SIM_par_1	107	113	PF11976	0.245
LIG_TYR_ITIM	102	107	PF00017	0.440
LIG_UBA3_1	186	193	PF00899	0.246
LIG_UBA3_1	210	217	PF00899	0.250
MOD_CK1_1	128	134	PF00069	0.396
MOD_CK1_1	153	159	PF00069	0.361
MOD_CK1_1	179	185	PF00069	0.393
MOD_CK1_1	203	209	PF00069	0.401
MOD_CK1_1	221	227	PF00069	0.377
MOD_CK1_1	245	251	PF00069	0.378
MOD_CK1_1	340	346	PF00069	0.267
MOD_CK1_1	80	86	PF00069	0.330
MOD_CK2_1	308	314	PF00069	0.384
MOD_CK2_1	36	42	PF00069	0.354
MOD_CK2_1	53	59	PF00069	0.293
MOD_CK2_1	91	97	PF00069	0.410
MOD_Cter_Amidation	60	63	PF01082	0.461
MOD_GlcNHglycan	113	117	PF01048	0.532
MOD_GlcNHglycan	121	124	PF01048	0.568
MOD_GlcNHglycan	170	173	PF01048	0.597
MOD_GlcNHglycan	194	197	PF01048	0.557
MOD_GlcNHglycan	218	221	PF01048	0.633
MOD_GlcNHglycan	225	229	PF01048	0.637
MOD_GlcNHglycan	230	233	PF01048	0.614
MOD_GlcNHglycan	241	245	PF01048	0.582
MOD_GlcNHglycan	25	28	PF01048	0.745
MOD_GlcNHglycan	250	253	PF01048	0.573
MOD_GlcNHglycan	324	327	PF01048	0.563
MOD_GlcNHglycan	356	359	PF01048	0.290
MOD_GlcNHglycan	79	82	PF01048	0.535
MOD_GSK3_1	121	128	PF00069	0.374
MOD_GSK3_1	130	137	PF00069	0.377
MOD_GSK3_1	146	153	PF00069	0.321
MOD_GSK3_1	176	183	PF00069	0.356
MOD_GSK3_1	200	207	PF00069	0.412
MOD_GSK3_1	224	231	PF00069	0.395
MOD_GSK3_1	242	249	PF00069	0.356
MOD_GSK3_1	336	343	PF00069	0.439
MOD_GSK3_1	76	83	PF00069	0.358
MOD_N-GLC_2	297	299	PF02516	0.544
MOD_NEK2_1	10	15	PF00069	0.601
MOD_NEK2_1	112	117	PF00069	0.368
MOD_NEK2_1	121	126	PF00069	0.428
MOD_NEK2_1	165	170	PF00069	0.337
MOD_NEK2_1	21	26	PF00069	0.578
MOD_NEK2_1	242	247	PF00069	0.365
MOD_NEK2_1	53	58	PF00069	0.349
MOD_PIKK_1	165	171	PF00454	0.259
MOD_PKA_2	119	125	PF00069	0.345
MOD_PKA_2	144	150	PF00069	0.384
MOD_Plk_1	112	118	PF00069	0.365
MOD_Plk_1	340	346	PF00069	0.435
MOD_Plk_4	131	137	PF00069	0.364
MOD_Plk_4	182	188	PF00069	0.382
MOD_Plk_4	206	212	PF00069	0.337
MOD_Plk_4	80	86	PF00069	0.435
MOD_ProDKin_1	301	307	PF00069	0.407
MOD_SUMO_rev_2	283	291	PF00179	0.303
TRG_DiLeu_BaEn_1	341	346	PF01217	0.271
TRG_ENDOCYTIC_2	104	107	PF00928	0.445
TRG_ER_diArg_1	5	7	PF00400	0.640
TRG_Pf-PMV_PEXEL_1	55	59	PF00026	0.511

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P276	Leptomonas seymouri	30%	100%
A0A0N0P2L4	Leptomonas seymouri	40%	100%
A0A0N0P4M2	Leptomonas seymouri	27%	100%
A0A0N1I121	Leptomonas seymouri	30%	75%
A0A0N1I661	Leptomonas seymouri	36%	66%
A0A0N1I7S5	Leptomonas seymouri	31%	74%
A0A0S4IZC7	Bodo saltans	24%	92%
A0A0S4J1A6	Bodo saltans	23%	87%
A0A0S4JBV9	Bodo saltans	27%	77%
A0A0S4JL29	Bodo saltans	32%	74%
A0A0S4KMV2	Bodo saltans	30%	96%
A0A3Q8I9A6	Leishmania donovani	83%	98%
A0A3Q8I9D0	Leishmania donovani	60%	100%
A0A3Q8IFC2	Leishmania donovani	38%	86%
A0A3Q8IIJ9	Leishmania donovani	35%	100%
A0A3Q8IK15	Leishmania donovani	37%	72%
A0A3S5H6D6	Leishmania donovani	29%	100%
A0A3S7WP69	Leishmania donovani	21%	100%
A0A3S7WPB2	Leishmania donovani	26%	100%
A0A3S7X4J4	Leishmania donovani	34%	100%
A4H4D2	Leishmania braziliensis	22%	100%
A4H4G6	Leishmania braziliensis	24%	100%
A4H5P0	Leishmania braziliensis	30%	100%
A4HBX3	Leishmania braziliensis	33%	83%
A4HJC8	Leishmania braziliensis	34%	100%
A4HJX1	Leishmania braziliensis	41%	86%
A4HSL2	Leishmania infantum	21%	100%
A4HTX9	Leishmania infantum	29%	100%
A4HVB0	Leishmania infantum	56%	100%
A4I6S2	Leishmania infantum	37%	86%
A4I6S3	Leishmania infantum	37%	75%
A4I6S4	Leishmania infantum	35%	100%
D1GJ51	Leishmania infantum	56%	100%
E8NHG5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	39%	85%
E8NHP8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
E9ACQ0	Leishmania major	27%	100%
E9AEF4	Leishmania major	41%	82%
E9AG65	Leishmania infantum	26%	100%
E9AGG5	Leishmania infantum	76%	100%
E9AGG9	Leishmania infantum	50%	70%
E9AKJ6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	21%	100%
E9AKM8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	100%
E9AMQ9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	96%
E9AP00	Leishmania mexicana (strain MHOM/GT/2001/U1103)	36%	99%
E9AP02	Leishmania mexicana (strain MHOM/GT/2001/U1103)	63%	93%
E9AP03	Leishmania mexicana (strain MHOM/GT/2001/U1103)	69%	100%
E9AP05	Leishmania mexicana (strain MHOM/GT/2001/U1103)	48%	73%
E9AP07	Leishmania mexicana (strain MHOM/GT/2001/U1103)	65%	94%
E9AP08	Leishmania mexicana (strain MHOM/GT/2001/U1103)	63%	100%
E9AVA1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	82%
E9B1U3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	41%	83%
E9B1U4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	33%	100%
E9B1U5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	39%	90%
E9B1U6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	100%
P21978	Leishmania amazonensis	48%	79%
P35334	Phaseolus vulgaris	20%	100%
P58822	Phaseolus vulgaris	21%	100%
Q25331	Leishmania major	55%	89%
Q4PSE6	Arabidopsis thaliana	23%	87%
Q4Q6B6	Leishmania major	35%	100%
Q4Q6B7	Leishmania major	35%	100%
Q4Q6B8	Leishmania major	38%	71%
Q4QGI0	Leishmania major	49%	74%
Q4QGI2	Leishmania major	45%	67%
Q4QGI4	Leishmania major	48%	70%
Q4QGI6	Leishmania major	48%	69%
Q4QGJ4	Leishmania major	55%	89%
Q4QGJ7	Leishmania major	56%	89%
Q4QGK0	Leishmania major	53%	66%
Q4QGK2	Leishmania major	51%	67%
Q4QGK6	Leishmania major	55%	89%
Q4QGK8	Leishmania major	50%	74%
Q4QGL2	Leishmania major	50%	74%
Q4QGL4	Leishmania major	64%	99%
Q4QGL5	Leishmania major	51%	69%
Q4QHW6	Leishmania major	28%	100%
Q4QJB2	Leishmania major	21%	100%