Putative surface antigen protein 2

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein - conserved

Species:

Leishmania infantum

UniProt:

E9AGG5_LEIIN

TriTrypDb:

LINF_120014400

Length:

417

Annotations

LeishMANIAdb annotations

Publication identifier(s): 8702946
A surface coat protein involved in immune evasion in Leishmaniids. Extremely fast evolving, almost completely disordered mucin-like protein. . Localization: Cell surface (experimental)

LeishMANIAdb interaction annotations

by homology
Contact email: handman@wehi.edu.au
Publication title: A Leucine-Rich Repeat Motif of Leishmania Parasite Surface Antigen 2 Binds to Macrophages through the Complement Receptor 3
Publication 1st author(s): Kedzierski
Publication Identifier(s): 15067069
Host organism: -1
Interaction detection method(s): fluorescence activated cell sorting
Interaction type: physical association
Identification method participant A: identification by antibody
Identification method participant B: identification by antibody
ID(s) interactor A: P11215
ID(s) interactor B: E9AGG4
Taxid interactor A: Homo sapiens
Taxid interactor B: Leishmania infantum
Biological role(s) interactor A: unspecified role
Biological role(s) interactor B: unspecified role
Experimental role(s) interactor A: unspecified role

Annotations by Jardim et al.

Plasma membrane, surface antigen 2 LINJ.12.0666

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 155

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 7

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 14

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	yes	yes: 62, no: 8
NetGPI	no	yes: 0, no: 70

Cellular components
Term	Name	Level	Count
GO:0005929	cilium	4	71
GO:0042995	cell projection	2	71
GO:0043226	organelle	2	71
GO:0043227	membrane-bounded organelle	3	71
GO:0110165	cellular anatomical entity	1	71
GO:0120025	plasma membrane bounded cell projection	3	71
GO:0016020	membrane	2	25
GO:0005886	plasma membrane	3	3

Expansion

Sequence features

E9AGG5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AGG5

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	1
GO:0004672	protein kinase activity	3	1
GO:0004674	protein serine/threonine kinase activity	4	1
GO:0016301	kinase activity	4	1
GO:0016740	transferase activity	2	1
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	1
GO:0016773	phosphotransferase activity, alcohol group as acceptor	4	1
GO:0140096	catalytic activity, acting on a protein	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	277	279	PF00675	0.508
CLV_NRD_NRD_1	6	8	PF00675	0.732
CLV_PCSK_KEX2_1	277	279	PF00082	0.523
CLV_PCSK_KEX2_1	316	318	PF00082	0.652
CLV_PCSK_KEX2_1	6	8	PF00082	0.732
CLV_PCSK_PC1ET2_1	277	279	PF00082	0.523
CLV_PCSK_PC1ET2_1	316	318	PF00082	0.638
CLV_PCSK_SKI1_1	257	261	PF00082	0.550
CLV_PCSK_SKI1_1	31	35	PF00082	0.720
CLV_PCSK_SKI1_1	7	11	PF00082	0.748
DEG_SCF_FBW7_2	345	350	PF00400	0.444
DEG_SPOP_SBC_1	303	307	PF00917	0.643
DEG_SPOP_SBC_1	320	324	PF00917	0.433
DOC_CYCLIN_RxL_1	3	13	PF00134	0.466
DOC_MAPK_gen_1	277	284	PF00069	0.311
DOC_MAPK_MEF2A_6	277	284	PF00069	0.359
DOC_USP7_MATH_1	318	322	PF00917	0.475
DOC_USP7_MATH_2	199	205	PF00917	0.349
DOC_USP7_UBL2_3	294	298	PF12436	0.346
DOC_USP7_UBL2_3	61	65	PF12436	0.294
DOC_WW_Pin1_4	337	342	PF00397	0.576
DOC_WW_Pin1_4	343	348	PF00397	0.511
LIG_Actin_WH2_2	177	192	PF00022	0.274
LIG_BIR_II_1	1	5	PF00653	0.664
LIG_FHA_1	103	109	PF00498	0.336
LIG_FHA_1	131	137	PF00498	0.336
LIG_FHA_1	145	151	PF00498	0.438
LIG_FHA_1	184	190	PF00498	0.321
LIG_FHA_1	325	331	PF00498	0.436
LIG_FHA_1	44	50	PF00498	0.457
LIG_FHA_2	108	114	PF00498	0.327
LIG_FHA_2	207	213	PF00498	0.382
LIG_FHA_2	349	355	PF00498	0.502
LIG_LIR_Gen_1	103	112	PF02991	0.429
LIG_LIR_Gen_1	128	136	PF02991	0.338
LIG_LIR_Gen_1	153	160	PF02991	0.431
LIG_LIR_Gen_1	200	210	PF02991	0.318
LIG_LIR_Gen_1	248	256	PF02991	0.402
LIG_LIR_Nem_3	103	107	PF02991	0.437
LIG_LIR_Nem_3	71	75	PF02991	0.358
LIG_PCNA_PIPBox_1	132	141	PF02747	0.264
LIG_PDZ_Class_2	412	417	PF00595	0.283
LIG_Pex14_1	72	76	PF04695	0.245
LIG_SH2_CRK	104	108	PF00017	0.481
LIG_SH2_CRK	76	80	PF00017	0.268
LIG_SH2_GRB2like	104	107	PF00017	0.273
LIG_SH2_STAP1	104	108	PF00017	0.280
LIG_SH2_STAT5	104	107	PF00017	0.400
LIG_SH2_STAT5	376	379	PF00017	0.324
LIG_SH2_STAT5	74	77	PF00017	0.376
LIG_SH3_1	298	304	PF00018	0.387
LIG_SH3_3	298	304	PF00018	0.393
LIG_SH3_3	329	335	PF00018	0.581
LIG_SH3_3	93	99	PF00018	0.427
LIG_SUMO_SIM_anti_2	206	212	PF11976	0.277
LIG_SUMO_SIM_anti_2	46	51	PF11976	0.312
LIG_SUMO_SIM_par_1	280	285	PF11976	0.321
LIG_TYR_ITIM	102	107	PF00017	0.351
LIG_UBA3_1	210	217	PF00899	0.296
MOD_CK1_1	128	134	PF00069	0.413
MOD_CK1_1	153	159	PF00069	0.486
MOD_CK1_1	179	185	PF00069	0.385
MOD_CK1_1	197	203	PF00069	0.351
MOD_CK1_1	204	210	PF00069	0.363
MOD_CK1_1	221	227	PF00069	0.426
MOD_CK1_1	245	251	PF00069	0.351
MOD_CK1_1	312	318	PF00069	0.459
MOD_CK1_1	321	327	PF00069	0.445
MOD_CK1_1	380	386	PF00069	0.355
MOD_CK2_1	348	354	PF00069	0.544
MOD_CK2_1	91	97	PF00069	0.415
MOD_GlcNHglycan	113	117	PF01048	0.588
MOD_GlcNHglycan	121	124	PF01048	0.583
MOD_GlcNHglycan	170	173	PF01048	0.551
MOD_GlcNHglycan	194	197	PF01048	0.633
MOD_GlcNHglycan	218	221	PF01048	0.641
MOD_GlcNHglycan	225	229	PF01048	0.605
MOD_GlcNHglycan	230	233	PF01048	0.571
MOD_GlcNHglycan	241	245	PF01048	0.526
MOD_GlcNHglycan	25	28	PF01048	0.764
MOD_GlcNHglycan	250	253	PF01048	0.561
MOD_GlcNHglycan	261	264	PF01048	0.554
MOD_GlcNHglycan	362	367	PF01048	0.724
MOD_GlcNHglycan	396	399	PF01048	0.290
MOD_GlcNHglycan	79	82	PF01048	0.591
MOD_GSK3_1	121	128	PF00069	0.435
MOD_GSK3_1	130	137	PF00069	0.374
MOD_GSK3_1	146	153	PF00069	0.456
MOD_GSK3_1	176	183	PF00069	0.367
MOD_GSK3_1	185	192	PF00069	0.324
MOD_GSK3_1	197	204	PF00069	0.354
MOD_GSK3_1	224	231	PF00069	0.403
MOD_GSK3_1	242	249	PF00069	0.353
MOD_GSK3_1	302	309	PF00069	0.508
MOD_GSK3_1	312	319	PF00069	0.483
MOD_GSK3_1	320	327	PF00069	0.475
MOD_GSK3_1	330	337	PF00069	0.627
MOD_GSK3_1	339	346	PF00069	0.521
MOD_GSK3_1	376	383	PF00069	0.448
MOD_N-GLC_1	36	41	PF02516	0.591
MOD_N-GLC_2	297	299	PF02516	0.686
MOD_NEK2_1	10	15	PF00069	0.528
MOD_NEK2_1	112	117	PF00069	0.444
MOD_NEK2_1	121	126	PF00069	0.410
MOD_NEK2_1	165	170	PF00069	0.341
MOD_NEK2_1	189	194	PF00069	0.420
MOD_NEK2_1	21	26	PF00069	0.528
MOD_NEK2_1	213	218	PF00069	0.317
MOD_NEK2_1	242	247	PF00069	0.365
MOD_NEK2_1	38	43	PF00069	0.361
MOD_NEK2_2	185	190	PF00069	0.363
MOD_PIKK_1	107	113	PF00454	0.252
MOD_PIKK_1	165	171	PF00454	0.326
MOD_PIKK_1	38	44	PF00454	0.311
MOD_PKA_1	316	322	PF00069	0.431
MOD_PKA_2	119	125	PF00069	0.295
MOD_PKA_2	144	150	PF00069	0.444
MOD_PKA_2	189	195	PF00069	0.281
MOD_PKA_2	316	322	PF00069	0.439
MOD_Plk_1	200	206	PF00069	0.357
MOD_Plk_1	380	386	PF00069	0.445
MOD_Plk_4	131	137	PF00069	0.372
MOD_Plk_4	206	212	PF00069	0.365
MOD_ProDKin_1	337	343	PF00069	0.573
TRG_DiLeu_BaEn_1	381	386	PF01217	0.319
TRG_ENDOCYTIC_2	104	107	PF00928	0.500
TRG_ER_diArg_1	5	7	PF00400	0.658
TRG_Pf-PMV_PEXEL_1	55	59	PF00026	0.512

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I121	Leptomonas seymouri	30%	83%
A0A0N1I7S5	Leptomonas seymouri	30%	82%
A0A0S4IZC7	Bodo saltans	25%	100%
A0A0S4J206	Bodo saltans	31%	68%
A0A0S4JB17	Bodo saltans	29%	67%
A0A0S4JEP2	Bodo saltans	21%	72%
A0A0S4KK37	Bodo saltans	25%	72%
A0A0S4KMS7	Bodo saltans	27%	100%
A0A3Q8I9A6	Leishmania donovani	81%	100%
A0A3Q8I9B4	Leishmania donovani	59%	67%
A0A3Q8I9D0	Leishmania donovani	60%	100%
A0A3Q8I9D9	Leishmania donovani	59%	66%
A0A3Q8IFC2	Leishmania donovani	38%	96%
A0A3Q8IIJ9	Leishmania donovani	30%	100%
A0A3S5H6D6	Leishmania donovani	30%	100%
A0A3S5H6L9	Leishmania donovani	59%	72%
A0A3S7WP69	Leishmania donovani	24%	100%
A0A3S7WPB2	Leishmania donovani	24%	100%
A0A3S7X4J4	Leishmania donovani	33%	100%
A4H4D2	Leishmania braziliensis	23%	100%
A4H4G6	Leishmania braziliensis	24%	100%
A4H5P0	Leishmania braziliensis	29%	100%
A4HBX3	Leishmania braziliensis	31%	92%
A4HJC8	Leishmania braziliensis	34%	100%
A4HSL2	Leishmania infantum	24%	100%
A4HTX9	Leishmania infantum	28%	100%
A4HVB0	Leishmania infantum	53%	100%
A4I6S2	Leishmania infantum	35%	100%
A4I6S3	Leishmania infantum	39%	83%
A4I6S4	Leishmania infantum	32%	100%
D1GJ51	Leishmania infantum	53%	90%
E8NHG5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	38%	94%
E8NHP8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
E9ACQ0	Leishmania major	24%	100%
E9AG65	Leishmania infantum	24%	100%
E9AGG7	Leishmania infantum	41%	67%
E9AGG9	Leishmania infantum	44%	77%
E9AGH0	Leishmania infantum	76%	100%
E9AKM8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	100%
E9AMQ9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	100%
E9AP02	Leishmania mexicana (strain MHOM/GT/2001/U1103)	58%	86%
E9AP03	Leishmania mexicana (strain MHOM/GT/2001/U1103)	63%	93%
E9AP05	Leishmania mexicana (strain MHOM/GT/2001/U1103)	48%	81%
E9AP07	Leishmania mexicana (strain MHOM/GT/2001/U1103)	59%	87%
E9AP08	Leishmania mexicana (strain MHOM/GT/2001/U1103)	68%	100%
E9AVA1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	36%	90%
E9B1U4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
E9B1U5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	40%	100%
E9B1U6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
Q25331	Leishmania major	56%	89%
Q4Q6B6	Leishmania major	34%	100%
Q4Q6B7	Leishmania major	33%	100%
Q4Q6B8	Leishmania major	35%	79%
Q4QGI0	Leishmania major	47%	82%
Q4QGI2	Leishmania major	48%	74%
Q4QGI4	Leishmania major	46%	78%
Q4QGI6	Leishmania major	61%	76%
Q4QGJ0	Leishmania major	52%	66%
Q4QGJ4	Leishmania major	56%	89%
Q4QGJ6	Leishmania major	49%	73%
Q4QGJ7	Leishmania major	57%	89%
Q4QGJ9	Leishmania major	51%	71%
Q4QGK2	Leishmania major	51%	74%
Q4QGK6	Leishmania major	56%	89%
Q4QGL4	Leishmania major	55%	90%
Q4QGL5	Leishmania major	49%	76%
Q4QGL8	Leishmania major	56%	68%
Q4QHW6	Leishmania major	29%	100%
Q4QJB2	Leishmania major	21%	100%