Diacylglycerol kinase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

diacylglycerol kinase-like protein

Species:

Leishmania major

UniProt:

E9AG10_LEIMA

TriTrypDb:

LmjF.35.5370 , LMJLV39_350062000 , LMJSD75_350061300

Length:

387

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 18

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 8
NetGPI	no	yes: 0, no: 8

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	6
GO:0110165	cellular anatomical entity	1	6

Expansion

Sequence features

E9AG10

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AG10

Function

Biological processes
Term	Name	Level	Count
GO:0006793	phosphorus metabolic process	3	9
GO:0006796	phosphate-containing compound metabolic process	4	9
GO:0007165	signal transduction	2	9
GO:0007186	G protein-coupled receptor signaling pathway	3	9
GO:0007205	protein kinase C-activating G protein-coupled receptor signaling pathway	4	9
GO:0008152	metabolic process	1	9
GO:0009987	cellular process	1	9
GO:0016310	phosphorylation	5	9
GO:0044237	cellular metabolic process	2	9
GO:0050789	regulation of biological process	2	9
GO:0050794	regulation of cellular process	3	9
GO:0065007	biological regulation	1	9

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	9
GO:0003824	catalytic activity	1	9
GO:0004143	diacylglycerol kinase activity	5	9
GO:0005488	binding	1	9
GO:0005524	ATP binding	5	9
GO:0016301	kinase activity	4	9
GO:0016740	transferase activity	2	9
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	9
GO:0016773	phosphotransferase activity, alcohol group as acceptor	4	9
GO:0017076	purine nucleotide binding	4	9
GO:0030554	adenyl nucleotide binding	5	9
GO:0032553	ribonucleotide binding	3	9
GO:0032555	purine ribonucleotide binding	4	9
GO:0032559	adenyl ribonucleotide binding	5	9
GO:0035639	purine ribonucleoside triphosphate binding	4	9
GO:0036094	small molecule binding	2	9
GO:0043167	ion binding	2	9
GO:0043168	anion binding	3	9
GO:0097159	organic cyclic compound binding	2	9
GO:0097367	carbohydrate derivative binding	2	9
GO:1901265	nucleoside phosphate binding	3	9
GO:1901363	heterocyclic compound binding	2	9

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	240	242	PF00675	0.350
CLV_NRD_NRD_1	28	30	PF00675	0.316
CLV_NRD_NRD_1	67	69	PF00675	0.373
CLV_PCSK_KEX2_1	137	139	PF00082	0.335
CLV_PCSK_KEX2_1	240	242	PF00082	0.299
CLV_PCSK_KEX2_1	28	30	PF00082	0.380
CLV_PCSK_KEX2_1	92	94	PF00082	0.339
CLV_PCSK_PC1ET2_1	137	139	PF00082	0.347
CLV_PCSK_PC1ET2_1	92	94	PF00082	0.339
CLV_PCSK_PC7_1	24	30	PF00082	0.329
CLV_PCSK_SKI1_1	15	19	PF00082	0.381
DEG_MDM2_SWIB_1	289	297	PF02201	0.535
DEG_ODPH_VHL_1	100	112	PF01847	0.534
DEG_SPOP_SBC_1	161	165	PF00917	0.601
DOC_MAPK_gen_1	306	314	PF00069	0.411
DOC_MAPK_MEF2A_6	307	316	PF00069	0.411
DOC_MAPK_MEF2A_6	60	67	PF00069	0.547
DOC_MAPK_NFAT4_5	60	68	PF00069	0.529
DOC_PP1_RVXF_1	297	303	PF00149	0.535
DOC_PP1_RVXF_1	341	348	PF00149	0.535
DOC_PP2B_LxvP_1	353	356	PF13499	0.574
DOC_USP7_MATH_1	275	279	PF00917	0.504
DOC_WW_Pin1_4	381	386	PF00397	0.465
DOC_WW_Pin1_4	4	9	PF00397	0.723
LIG_14-3-3_CanoR_1	156	160	PF00244	0.555
LIG_14-3-3_CanoR_1	28	35	PF00244	0.562
LIG_APCC_ABBA_1	221	226	PF00400	0.526
LIG_BRCT_BRCA1_1	182	186	PF00533	0.477
LIG_BRCT_BRCA1_1	321	325	PF00533	0.526
LIG_Clathr_ClatBox_1	234	238	PF01394	0.573
LIG_deltaCOP1_diTrp_1	45	51	PF00928	0.573
LIG_FHA_1	146	152	PF00498	0.588
LIG_FHA_1	205	211	PF00498	0.535
LIG_FHA_1	311	317	PF00498	0.573
LIG_FHA_1	339	345	PF00498	0.544
LIG_FHA_2	112	118	PF00498	0.477
LIG_FHA_2	150	156	PF00498	0.580
LIG_GBD_Chelix_1	373	381	PF00786	0.337
LIG_LIR_Apic_2	272	277	PF02991	0.535
LIG_LIR_Gen_1	136	146	PF02991	0.633
LIG_LIR_Gen_1	16	23	PF02991	0.526
LIG_LIR_Gen_1	361	372	PF02991	0.467
LIG_LIR_Gen_1	45	56	PF02991	0.535
LIG_LIR_Gen_1	73	78	PF02991	0.494
LIG_LIR_Nem_3	136	142	PF02991	0.584
LIG_LIR_Nem_3	16	21	PF02991	0.515
LIG_LIR_Nem_3	175	181	PF02991	0.576
LIG_LIR_Nem_3	340	345	PF02991	0.530
LIG_LIR_Nem_3	361	367	PF02991	0.467
LIG_LIR_Nem_3	45	51	PF02991	0.498
LIG_LIR_Nem_3	73	77	PF02991	0.494
LIG_PCNA_TLS_4	60	67	PF02747	0.573
LIG_Pex14_1	214	218	PF04695	0.477
LIG_Pex14_2	120	124	PF04695	0.533
LIG_Pex14_2	178	182	PF04695	0.490
LIG_Pex14_2	289	293	PF04695	0.535
LIG_PTB_Apo_2	176	183	PF02174	0.535
LIG_SH2_CRK	274	278	PF00017	0.535
LIG_SH2_CRK	382	386	PF00017	0.468
LIG_SH2_NCK_1	146	150	PF00017	0.595
LIG_SH2_NCK_1	274	278	PF00017	0.526
LIG_SH2_PTP2	74	77	PF00017	0.573
LIG_SH2_SRC	74	77	PF00017	0.573
LIG_SH2_STAP1	215	219	PF00017	0.535
LIG_SH2_STAT5	181	184	PF00017	0.474
LIG_SH2_STAT5	33	36	PF00017	0.505
LIG_SH2_STAT5	74	77	PF00017	0.563
LIG_SH3_3	103	109	PF00018	0.547
LIG_SH3_3	96	102	PF00018	0.516
LIG_SUMO_SIM_par_1	231	238	PF11976	0.573
LIG_TRAF2_1	357	360	PF00917	0.641
LIG_WW_3	355	359	PF00397	0.628
MOD_CDK_SPK_2	4	9	PF00069	0.723
MOD_CK1_1	247	253	PF00069	0.573
MOD_CK2_1	345	351	PF00069	0.569
MOD_CK2_1	58	64	PF00069	0.573
MOD_Cter_Amidation	238	241	PF01082	0.299
MOD_Cter_Amidation	26	29	PF01082	0.329
MOD_Cter_Amidation	304	307	PF01082	0.335
MOD_GlcNHglycan	125	128	PF01048	0.379
MOD_GlcNHglycan	169	172	PF01048	0.433
MOD_GlcNHglycan	24	27	PF01048	0.269
MOD_GlcNHglycan	246	249	PF01048	0.302
MOD_GSK3_1	145	152	PF00069	0.590
MOD_GSK3_1	155	162	PF00069	0.544
MOD_GSK3_1	315	322	PF00069	0.535
MOD_GSK3_1	333	340	PF00069	0.468
MOD_GSK3_1	347	354	PF00069	0.444
MOD_NEK2_1	118	123	PF00069	0.498
MOD_NEK2_1	159	164	PF00069	0.597
MOD_NEK2_1	172	177	PF00069	0.626
MOD_NEK2_1	204	209	PF00069	0.520
MOD_NEK2_1	38	43	PF00069	0.546
MOD_NEK2_2	13	18	PF00069	0.605
MOD_NEK2_2	338	343	PF00069	0.621
MOD_PIKK_1	58	64	PF00454	0.500
MOD_PKA_1	28	34	PF00069	0.565
MOD_PKA_2	155	161	PF00069	0.532
MOD_PKA_2	27	33	PF00069	0.519
MOD_Plk_1	116	122	PF00069	0.526
MOD_Plk_4	229	235	PF00069	0.573
MOD_Plk_4	275	281	PF00069	0.529
MOD_ProDKin_1	381	387	PF00069	0.469
MOD_ProDKin_1	4	10	PF00069	0.720
TRG_ENDOCYTIC_2	181	184	PF00928	0.482
TRG_ENDOCYTIC_2	215	218	PF00928	0.468
TRG_ENDOCYTIC_2	74	77	PF00928	0.563
TRG_ER_diArg_1	197	200	PF00400	0.573
TRG_ER_diArg_1	240	242	PF00400	0.499

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IKR4	Leptomonas seymouri	68%	100%
A0A0S4KIS7	Bodo saltans	24%	87%
A0A1X0NZZ0	Trypanosomatidae	36%	100%
A0A3Q8IIW3	Leishmania donovani	95%	100%
A4HNJ8	Leishmania braziliensis	80%	100%
A4IC67	Leishmania infantum	95%	100%
C9ZVX0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	24%	67%
E9B763	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
P52429	Homo sapiens	31%	68%
Q1PDI2	Arabidopsis thaliana	24%	79%
Q7XQT2	Oryza sativa subsp. japonica	24%	78%
Q9C5E5	Arabidopsis thaliana	25%	76%
Q9R1C6	Mus musculus	31%	69%
V5AWH3	Trypanosoma cruzi	36%	100%