GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

alpha-1,2-mannosyltransferase, putative

Species:

Leishmania major

UniProt:

E9AFZ8_LEIMA

TriTrypDb:

LmjF.35.5250 , LMJLV39_350060700 , LMJSD75_350060000

Length:

466

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005789	endoplasmic reticulum membrane	4	6
GO:0016020	membrane	2	12
GO:0031090	organelle membrane	3	6
GO:0110165	cellular anatomical entity	1	12

Expansion

Sequence features

E9AFZ8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFZ8

Function

Biological processes
Term	Name	Level	Count
GO:0006486	protein glycosylation	4	6
GO:0006487	protein N-linked glycosylation	5	2
GO:0006490	oligosaccharide-lipid intermediate biosynthetic process	4	2
GO:0006629	lipid metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	6
GO:0008152	metabolic process	1	6
GO:0009058	biosynthetic process	2	2
GO:0009987	cellular process	1	2
GO:0019538	protein metabolic process	3	6
GO:0036211	protein modification process	4	6
GO:0043170	macromolecule metabolic process	3	6
GO:0043412	macromolecule modification	4	6
GO:0043413	macromolecule glycosylation	3	6
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	6
GO:0044255	cellular lipid metabolic process	3	2
GO:0070085	glycosylation	2	6
GO:0071704	organic substance metabolic process	2	6
GO:1901135	carbohydrate derivative metabolic process	3	2
GO:1901137	carbohydrate derivative biosynthetic process	4	2
GO:1901564	organonitrogen compound metabolic process	3	6
GO:1901576	organic substance biosynthetic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0000026	alpha-1,2-mannosyltransferase activity	6	12
GO:0000030	mannosyltransferase activity	5	12
GO:0003824	catalytic activity	1	12
GO:0004376	glycolipid mannosyltransferase activity	6	12
GO:0004377	GDP-Man:Man3GlcNAc2-PP-Dol alpha-1,2-mannosyltransferase activity	7	12
GO:0016740	transferase activity	2	12
GO:0016757	glycosyltransferase activity	3	12
GO:0016758	hexosyltransferase activity	4	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	18	20	PF00675	0.388
CLV_NRD_NRD_1	322	324	PF00675	0.361
CLV_PCSK_KEX2_1	20	22	PF00082	0.409
CLV_PCSK_KEX2_1	324	326	PF00082	0.316
CLV_PCSK_KEX2_1	437	439	PF00082	0.352
CLV_PCSK_KEX2_1	62	64	PF00082	0.327
CLV_PCSK_PC1ET2_1	20	22	PF00082	0.502
CLV_PCSK_PC1ET2_1	324	326	PF00082	0.334
CLV_PCSK_PC1ET2_1	437	439	PF00082	0.358
CLV_PCSK_PC1ET2_1	62	64	PF00082	0.380
CLV_PCSK_SKI1_1	109	113	PF00082	0.344
CLV_PCSK_SKI1_1	209	213	PF00082	0.357
CLV_PCSK_SKI1_1	307	311	PF00082	0.369
CLV_PCSK_SKI1_1	59	63	PF00082	0.328
DEG_Nend_Nbox_1	1	3	PF02207	0.492
DOC_CYCLIN_yCln2_LP_2	99	105	PF00134	0.315
DOC_MAPK_FxFP_2	159	162	PF00069	0.377
DOC_MAPK_gen_1	286	295	PF00069	0.315
DOC_MAPK_gen_1	304	314	PF00069	0.300
DOC_MAPK_gen_1	323	331	PF00069	0.320
DOC_MAPK_gen_1	59	68	PF00069	0.369
DOC_MAPK_gen_1	98	108	PF00069	0.355
DOC_MAPK_MEF2A_6	390	398	PF00069	0.385
DOC_PP1_RVXF_1	41	47	PF00149	0.300
DOC_PP1_RVXF_1	429	436	PF00149	0.447
DOC_PP1_RVXF_1	99	106	PF00149	0.420
DOC_PP2B_LxvP_1	256	259	PF13499	0.405
DOC_PP4_FxxP_1	159	162	PF00568	0.377
DOC_USP7_MATH_1	179	183	PF00917	0.369
DOC_USP7_MATH_1	266	270	PF00917	0.519
DOC_USP7_MATH_1	80	84	PF00917	0.418
DOC_WW_Pin1_4	145	150	PF00397	0.315
DOC_WW_Pin1_4	196	201	PF00397	0.362
DOC_WW_Pin1_4	398	403	PF00397	0.357
LIG_14-3-3_CanoR_1	123	127	PF00244	0.400
LIG_14-3-3_CanoR_1	25	29	PF00244	0.437
LIG_14-3-3_CanoR_1	278	283	PF00244	0.426
LIG_Actin_WH2_2	93	111	PF00022	0.413
LIG_BIR_III_2	119	123	PF00653	0.447
LIG_eIF4E_1	121	127	PF01652	0.300
LIG_eIF4E_1	161	167	PF01652	0.315
LIG_eIF4E_1	216	222	PF01652	0.385
LIG_FHA_1	1	7	PF00498	0.471
LIG_FHA_1	111	117	PF00498	0.308
LIG_FHA_1	123	129	PF00498	0.263
LIG_FHA_1	146	152	PF00498	0.323
LIG_FHA_1	184	190	PF00498	0.333
LIG_FHA_1	451	457	PF00498	0.475
LIG_FHA_2	347	353	PF00498	0.408
LIG_FHA_2	364	370	PF00498	0.315
LIG_FHA_2	408	414	PF00498	0.335
LIG_LIR_Gen_1	3	12	PF02991	0.477
LIG_LIR_Gen_1	403	414	PF02991	0.420
LIG_LIR_Gen_1	453	463	PF02991	0.491
LIG_LIR_Nem_3	119	124	PF02991	0.351
LIG_LIR_Nem_3	174	178	PF02991	0.322
LIG_LIR_Nem_3	3	7	PF02991	0.489
LIG_LIR_Nem_3	369	375	PF02991	0.315
LIG_LIR_Nem_3	403	409	PF02991	0.386
LIG_LIR_Nem_3	430	435	PF02991	0.385
LIG_LIR_Nem_3	453	458	PF02991	0.474
LIG_LYPXL_yS_3	121	124	PF13949	0.334
LIG_LYPXL_yS_3	161	164	PF13949	0.300
LIG_LYPXL_yS_3	178	181	PF13949	0.300
LIG_NRBOX	123	129	PF00104	0.377
LIG_PDZ_Class_2	461	466	PF00595	0.466
LIG_Pex14_2	451	455	PF04695	0.364
LIG_SH2_SRC	462	465	PF00017	0.503
LIG_SH2_STAP1	139	143	PF00017	0.334
LIG_SH2_STAP1	414	418	PF00017	0.334
LIG_SH2_STAT5	107	110	PF00017	0.367
LIG_SH2_STAT5	175	178	PF00017	0.300
LIG_SH2_STAT5	379	382	PF00017	0.357
LIG_SH2_STAT5	432	435	PF00017	0.377
LIG_SH2_STAT5	462	465	PF00017	0.503
LIG_SH2_STAT5	65	68	PF00017	0.300
LIG_SH3_3	152	158	PF00018	0.300
LIG_SH3_3	300	306	PF00018	0.315
LIG_SUMO_SIM_anti_2	339	346	PF11976	0.213
LIG_SUMO_SIM_anti_2	395	401	PF11976	0.447
LIG_SUMO_SIM_anti_2	5	10	PF11976	0.475
LIG_SUMO_SIM_par_1	124	130	PF11976	0.338
LIG_SUMO_SIM_par_1	148	154	PF11976	0.346
LIG_SUMO_SIM_par_1	373	378	PF11976	0.315
LIG_SUMO_SIM_par_1	417	423	PF11976	0.380
LIG_SUMO_SIM_par_1	86	92	PF11976	0.447
LIG_TRAF2_1	410	413	PF00917	0.336
LIG_TYR_ITIM	213	218	PF00017	0.292
LIG_TYR_ITSM	174	181	PF00017	0.315
LIG_UBA3_1	87	93	PF00899	0.408
LIG_Vh1_VBS_1	207	225	PF01044	0.447
LIG_WRC_WIRS_1	1	6	PF05994	0.480
LIG_WRC_WIRS_1	128	133	PF05994	0.415
LIG_WRC_WIRS_1	443	448	PF05994	0.457
MOD_CK1_1	183	189	PF00069	0.361
MOD_CK1_1	237	243	PF00069	0.300
MOD_CK1_1	450	456	PF00069	0.353
MOD_CK1_1	79	85	PF00069	0.353
MOD_CK2_1	346	352	PF00069	0.408
MOD_CK2_1	407	413	PF00069	0.316
MOD_CK2_1	86	92	PF00069	0.390
MOD_GlcNHglycan	116	119	PF01048	0.379
MOD_GlcNHglycan	182	185	PF01048	0.362
MOD_GlcNHglycan	190	193	PF01048	0.300
MOD_GlcNHglycan	390	393	PF01048	0.299
MOD_GlcNHglycan	78	81	PF01048	0.432
MOD_GSK3_1	110	117	PF00069	0.385
MOD_GSK3_1	179	186	PF00069	0.344
MOD_GSK3_1	188	195	PF00069	0.314
MOD_GSK3_1	442	449	PF00069	0.505
MOD_GSK3_1	76	83	PF00069	0.399
MOD_N-GLC_1	235	240	PF02516	0.357
MOD_N-GLC_1	346	351	PF02516	0.367
MOD_NEK2_1	127	132	PF00069	0.364
MOD_NEK2_1	171	176	PF00069	0.308
MOD_NEK2_1	2	7	PF00069	0.433
MOD_NEK2_1	234	239	PF00069	0.303
MOD_NEK2_1	446	451	PF00069	0.474
MOD_NEK2_2	363	368	PF00069	0.447
MOD_PIKK_1	183	189	PF00454	0.385
MOD_PIKK_1	237	243	PF00454	0.367
MOD_PIKK_1	266	272	PF00454	0.468
MOD_PK_1	278	284	PF00069	0.447
MOD_PK_1	93	99	PF00069	0.369
MOD_PKA_1	437	443	PF00069	0.435
MOD_PKA_2	122	128	PF00069	0.361
MOD_PKA_2	24	30	PF00069	0.385
MOD_PKA_2	437	443	PF00069	0.367
MOD_PKA_2	76	82	PF00069	0.447
MOD_Plk_1	235	241	PF00069	0.357
MOD_Plk_1	346	352	PF00069	0.367
MOD_Plk_1	446	452	PF00069	0.353
MOD_Plk_4	122	128	PF00069	0.315
MOD_Plk_4	243	249	PF00069	0.437
MOD_Plk_4	86	92	PF00069	0.416
MOD_ProDKin_1	145	151	PF00069	0.315
MOD_ProDKin_1	196	202	PF00069	0.362
MOD_ProDKin_1	398	404	PF00069	0.357
MOD_SUMO_rev_2	321	326	PF00179	0.337
TRG_DiLeu_BaEn_1	340	345	PF01217	0.333
TRG_DiLeu_BaLyEn_6	159	164	PF01217	0.300
TRG_DiLeu_BaLyEn_6	95	100	PF01217	0.439
TRG_ENDOCYTIC_2	121	124	PF00928	0.300
TRG_ENDOCYTIC_2	161	164	PF00928	0.306
TRG_ENDOCYTIC_2	178	181	PF00928	0.286
TRG_ENDOCYTIC_2	215	218	PF00928	0.307
TRG_ENDOCYTIC_2	223	226	PF00928	0.300
TRG_ENDOCYTIC_2	432	435	PF00928	0.315
TRG_ENDOCYTIC_2	65	68	PF00928	0.408
TRG_ER_diArg_1	424	427	PF00400	0.438
TRG_ER_diArg_1	451	454	PF00400	0.450
TRG_Pf-PMV_PEXEL_1	114	119	PF00026	0.357

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P6F7	Leptomonas seymouri	66%	100%
A0A0S4JLQ0	Bodo saltans	42%	99%
A0A1X0P5V7	Trypanosomatidae	51%	98%
A0A3R7K7Z3	Trypanosoma rangeli	50%	98%
A0A3S7XA76	Leishmania donovani	94%	100%
A4HNI6	Leishmania braziliensis	87%	100%
A4IC43	Leishmania infantum	94%	100%
C9ZYD9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	53%	99%
E9B751	Leishmania mexicana (strain MHOM/GT/2001/U1103)	94%	100%
O74878	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	32%	99%
P53954	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	30%	85%
P53993	Caenorhabditis elegans	32%	99%
Q08B22	Xenopus laevis	37%	96%
Q2TAA5	Homo sapiens	37%	95%
Q3TZM9	Mus musculus	36%	95%
Q54DM9	Dictyostelium discoideum	35%	92%
Q59S72	Candida albicans (strain SC5314 / ATCC MYA-2876)	30%	77%
Q5R7Z6	Pongo abelii	37%	95%
Q6BVB2	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	31%	76%
Q6C9T3	Yarrowia lipolytica (strain CLIB 122 / E 150)	35%	73%
Q6CLD6	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	29%	82%
Q6FWD1	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	34%	92%
Q6P312	Xenopus tropicalis	37%	96%
Q75B12	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	33%	80%
Q7ZW24	Danio rerio	37%	93%
Q8X092	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	32%	84%
Q9XEE9	Arabidopsis thaliana	38%	100%
V5BPD4	Trypanosoma cruzi	51%	98%