tRNA (guanine-N(7)-)-methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tRNA (guanine-N(7)-)-methyltransferase, putative

Species:

Leishmania major

UniProt:

E9AFZ6_LEIMA

TriTrypDb:

LmjF.35.5230 , LMJLV39_350060500 , LMJSD75_350059800

Length:

241

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	12
GO:0032991	protein-containing complex	1	2
GO:0034708	methyltransferase complex	4	2
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0043229	intracellular organelle	3	12
GO:0043231	intracellular membrane-bounded organelle	4	12
GO:0043527	tRNA methyltransferase complex	5	2
GO:0110165	cellular anatomical entity	1	12
GO:1902494	catalytic complex	2	2
GO:1990234	transferase complex	3	2

Expansion

Sequence features

E9AFZ6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFZ6

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	2
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006396	RNA processing	6	2
GO:0006399	tRNA metabolic process	7	2
GO:0006400	tRNA modification	6	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008033	tRNA processing	8	2
GO:0008152	metabolic process	1	2
GO:0009451	RNA modification	5	2
GO:0009987	cellular process	1	2
GO:0016070	RNA metabolic process	5	2
GO:0030488	tRNA methylation	5	2
GO:0032259	methylation	2	2
GO:0034470	ncRNA processing	7	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0034660	ncRNA metabolic process	6	2
GO:0036265	RNA (guanine-N7)-methylation	5	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043412	macromolecule modification	4	2
GO:0043414	macromolecule methylation	3	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0046483	heterocycle metabolic process	3	2
GO:0071704	organic substance metabolic process	2	2
GO:0090304	nucleic acid metabolic process	4	2
GO:1901360	organic cyclic compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0000049	tRNA binding	5	12
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	12
GO:0008168	methyltransferase activity	4	12
GO:0008173	RNA methyltransferase activity	4	12
GO:0008175	tRNA methyltransferase activity	5	12
GO:0008176	tRNA (guanine-N7-)-methyltransferase activity	7	12
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	12
GO:0016423	tRNA (guanine) methyltransferase activity	6	12
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140101	catalytic activity, acting on a tRNA	4	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	146	148	PF00675	0.311
CLV_NRD_NRD_1	152	154	PF00675	0.300
CLV_NRD_NRD_1	197	199	PF00675	0.322
CLV_NRD_NRD_1	227	229	PF00675	0.475
CLV_NRD_NRD_1	39	41	PF00675	0.301
CLV_PCSK_KEX2_1	146	148	PF00082	0.300
CLV_PCSK_KEX2_1	151	153	PF00082	0.286
CLV_PCSK_KEX2_1	197	199	PF00082	0.320
CLV_PCSK_KEX2_1	39	41	PF00082	0.418
CLV_PCSK_PC1ET2_1	146	148	PF00082	0.311
CLV_PCSK_PC7_1	147	153	PF00082	0.322
CLV_PCSK_SKI1_1	135	139	PF00082	0.325
CLV_PCSK_SKI1_1	210	214	PF00082	0.345
DOC_CKS1_1	204	209	PF01111	0.337
DOC_CYCLIN_RxL_1	91	101	PF00134	0.366
DOC_MAPK_FxFP_2	138	141	PF00069	0.306
DOC_PP1_RVXF_1	109	116	PF00149	0.363
DOC_PP1_RVXF_1	133	139	PF00149	0.325
DOC_PP1_RVXF_1	84	91	PF00149	0.322
DOC_PP4_FxxP_1	138	141	PF00568	0.306
DOC_USP7_UBL2_3	229	233	PF12436	0.420
DOC_WW_Pin1_4	153	158	PF00397	0.306
DOC_WW_Pin1_4	190	195	PF00397	0.427
DOC_WW_Pin1_4	203	208	PF00397	0.427
LIG_14-3-3_CanoR_1	39	48	PF00244	0.442
LIG_BIR_II_1	1	5	PF00653	0.635
LIG_BRCT_BRCA1_1	124	128	PF00533	0.320
LIG_BRCT_BRCA1_1	133	137	PF00533	0.371
LIG_BRCT_BRCA1_1	192	196	PF00533	0.427
LIG_BRCT_BRCA1_2	124	130	PF00533	0.306
LIG_FHA_2	173	179	PF00498	0.306
LIG_LIR_Gen_1	38	49	PF02991	0.459
LIG_LIR_Gen_1	54	62	PF02991	0.254
LIG_LIR_Nem_3	107	113	PF02991	0.311
LIG_LIR_Nem_3	134	140	PF02991	0.365
LIG_LIR_Nem_3	193	199	PF02991	0.412
LIG_LIR_Nem_3	38	44	PF02991	0.462
LIG_LIR_Nem_3	54	58	PF02991	0.254
LIG_LYPXL_S_1	44	48	PF13949	0.420
LIG_LYPXL_yS_3	45	48	PF13949	0.428
LIG_Pex14_2	37	41	PF04695	0.531
LIG_PTB_Apo_2	109	116	PF02174	0.306
LIG_SH2_STAP1	163	167	PF00017	0.306
LIG_SH2_STAT5	174	177	PF00017	0.391
LIG_SH3_3	28	34	PF00018	0.430
LIG_SUMO_SIM_par_1	211	217	PF11976	0.427
LIG_TRAF2_1	2	5	PF00917	0.689
LIG_UBA3_1	78	86	PF00899	0.323
LIG_WRC_WIRS_1	87	92	PF05994	0.453
MOD_CDK_SPxxK_3	190	197	PF00069	0.427
MOD_CDK_SPxxK_3	203	210	PF00069	0.427
MOD_GSK3_1	122	129	PF00069	0.376
MOD_NEK2_1	172	177	PF00069	0.306
MOD_NEK2_1	65	70	PF00069	0.363
MOD_PIKK_1	104	110	PF00454	0.399
MOD_PKA_1	39	45	PF00069	0.409
MOD_PKA_2	39	45	PF00069	0.447
MOD_PKA_2	7	13	PF00069	0.656
MOD_ProDKin_1	153	159	PF00069	0.306
MOD_ProDKin_1	190	196	PF00069	0.427
MOD_ProDKin_1	203	209	PF00069	0.427
MOD_SUMO_rev_2	203	212	PF00179	0.376
TRG_ENDOCYTIC_2	110	113	PF00928	0.391
TRG_ENDOCYTIC_2	45	48	PF00928	0.433
TRG_ER_diArg_1	14	17	PF00400	0.495
TRG_ER_diArg_1	150	153	PF00400	0.306
TRG_ER_diArg_1	196	198	PF00400	0.322
TRG_ER_diArg_1	39	41	PF00400	0.443
TRG_NLS_MonoExtN_4	143	150	PF00514	0.302
TRG_Pf-PMV_PEXEL_1	197	201	PF00026	0.305

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P618	Leptomonas seymouri	85%	100%
A0A0S4JLN4	Bodo saltans	66%	92%
A0A1X0P519	Trypanosomatidae	69%	95%
A0A3Q8IGS8	Leishmania donovani	97%	100%
A0A422N5W0	Trypanosoma rangeli	71%	100%
A1CIF1	Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107)	30%	71%
A1CWA9	Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181)	29%	67%
A1TY08	Marinobacter nauticus (strain ATCC 700491 / DSM 11845 / VT8)	25%	100%
A2BP39	Prochlorococcus marinus (strain AS9601)	28%	100%
A2BUM1	Prochlorococcus marinus (strain MIT 9515)	28%	100%
A2Q9E4	Aspergillus niger (strain CBS 513.88 / FGSC A1513)	37%	81%
A2RM55	Lactococcus lactis subsp. cremoris (strain MG1363)	26%	100%
A2YB34	Oryza sativa subsp. indica	40%	93%
A3CQ23	Streptococcus sanguinis (strain SK36)	25%	100%
A3LS77	Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545)	38%	80%
A4HNI5	Leishmania braziliensis	89%	100%
A4IC41	Leishmania infantum	98%	100%
A5DC23	Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324)	37%	89%
A5E7T4	Lodderomyces elongisporus (strain ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239)	40%	79%
A5WAD6	Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1)	22%	100%
A6S8E7	Botryotinia fuckeliana (strain B05.10)	34%	85%
A6ZXD2	Saccharomyces cerevisiae (strain YJM789)	37%	84%
A7TT36	Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294 / BCRC 21397 / CBS 2163 / NBRC 10782 / NRRL Y-8283 / UCD 57-17)	34%	84%
A8G2P9	Prochlorococcus marinus (strain MIT 9215)	25%	100%
A8NFF0	Brugia malayi	41%	93%
A8WTA7	Caenorhabditis briggsae	41%	94%
A9UMM1	Xenopus tropicalis	39%	100%
B0JUK5	Microcystis aeruginosa (strain NIES-843 / IAM M-2473)	30%	100%
B0KN57	Pseudomonas putida (strain GB-1)	22%	100%
B0WSB8	Culex quinquefasciatus	38%	100%
B0Y4I9	Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163)	28%	67%
B1J2G9	Pseudomonas putida (strain W619)	23%	100%
B2AR91	Podospora anserina (strain S / ATCC MYA-4624 / DSM 980 / FGSC 10383)	37%	85%
B3LH81	Saccharomyces cerevisiae (strain RM11-1a)	37%	84%
B3MYY4	Drosophila ananassae	36%	94%
B3P8V8	Drosophila erecta	38%	94%
B4H4I3	Drosophila persimilis	38%	98%
B4I9N7	Drosophila sechellia	38%	94%
B4JLU7	Drosophila grimshawi	38%	98%
B4L529	Drosophila mojavensis	39%	97%
B4M703	Drosophila virilis	39%	98%
B4N278	Drosophila willistoni	37%	96%
B4Q1B6	Drosophila yakuba	38%	94%
B4R338	Drosophila simulans	38%	94%
B6SHG7	Zea mays	39%	95%
C3K3L6	Pseudomonas fluorescens (strain SBW25)	24%	100%
C9ZYD2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	69%	99%
E9B749	Leishmania mexicana (strain MHOM/GT/2001/U1103)	96%	100%
O77263	Drosophila melanogaster	38%	94%
P0CS80	Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565)	36%	84%
P0CS81	Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)	36%	84%
P72546	Synechococcus elongatus (strain PCC 7942 / FACHB-805)	28%	100%
P73161	Synechocystis sp. (strain PCC 6803 / Kazusa)	29%	100%
Q0CT71	Aspergillus terreus (strain NIH 2624 / FGSC A1156)	30%	70%
Q0IEN3	Aedes aegypti	37%	98%
Q0TZT0	Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173)	34%	85%
Q0VLC7	Alcanivorax borkumensis (strain ATCC 700651 / DSM 11573 / NCIMB 13689 / SK2)	26%	100%
Q117P8	Trichodesmium erythraeum (strain IMS101)	33%	100%
Q12009	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	37%	84%
Q15ZS8	Pseudoalteromonas atlantica (strain T6c / ATCC BAA-1087)	25%	100%
Q1HPU2	Bombyx mori	38%	100%
Q1IGD2	Pseudomonas entomophila (strain L48)	23%	100%
Q21EN2	Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024)	25%	100%
Q23126	Caenorhabditis elegans	41%	94%
Q28H76	Xenopus tropicalis	39%	88%
Q29I16	Drosophila pseudoobscura pseudoobscura	38%	98%
Q2GS86	Chaetomium globosum (strain ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970)	40%	82%
Q2JJQ0	Synechococcus sp. (strain JA-2-3B'a(2-13))	32%	100%
Q2JRH1	Synechococcus sp. (strain JA-3-3Ab)	33%	100%
Q2UU72	Aspergillus oryzae (strain ATCC 42149 / RIB 40)	31%	73%
Q2YDF1	Bos taurus	37%	87%
Q31CU2	Prochlorococcus marinus (strain MIT 9312)	26%	100%
Q3K588	Pseudomonas fluorescens (strain Pf0-1)	24%	100%
Q3M3Q5	Trichormus variabilis (strain ATCC 29413 / PCC 7937)	29%	100%
Q47WM2	Colwellia psychrerythraea (strain 34H / ATCC BAA-681)	24%	99%
Q48CM0	Pseudomonas savastanoi pv. phaseolicola (strain 1448A / Race 6)	23%	99%
Q4K4C6	Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)	24%	100%
Q4WQB9	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)	29%	67%
Q4ZM56	Pseudomonas syringae pv. syringae (strain B728a)	24%	99%
Q551M3	Dictyostelium discoideum	36%	87%
Q5A692	Candida albicans (strain SC5314 / ATCC MYA-2876)	36%	74%
Q5H737	Colletotrichum orbiculare (strain 104-T / ATCC 96160 / CBS 514.97 / LARS 414 / MAFF 240422)	34%	84%
Q5N2X5	Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1)	28%	100%
Q5XJ57	Danio rerio	39%	100%
Q67V78	Oryza sativa subsp. japonica	40%	93%
Q6BX78	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	35%	83%
Q6CE12	Yarrowia lipolytica (strain CLIB 122 / E 150)	43%	83%
Q6CY47	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	36%	87%
Q6FNR1	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	38%	85%
Q6NU94	Xenopus laevis	38%	88%
Q74ZK8	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	36%	85%
Q7Q2P7	Anopheles gambiae	38%	100%
Q7RZC1	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	36%	82%
Q7V350	Prochlorococcus marinus subsp. pastoris (strain CCMP1986 / NIES-2087 / MED4)	28%	100%
Q7VDU8	Prochlorococcus marinus (strain SARG / CCMP1375 / SS120)	26%	100%
Q88AF5	Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)	24%	99%
Q88CS7	Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)	22%	100%
Q8DHH6	Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1)	32%	100%
Q8GXB7	Arabidopsis thaliana	44%	96%
Q8YVX4	Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)	29%	100%
Q96WV1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	40%	88%
Q9CHI2	Lactococcus lactis subsp. lactis (strain IL1403)	26%	100%
Q9UBP6	Homo sapiens	36%	87%
Q9Z120	Mus musculus	38%	90%
V5B8T4	Trypanosoma cruzi	69%	100%