G domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

GTP-binding protein, putative

Species:

Leishmania major

UniProt:

E9AFZ4_LEIMA

TriTrypDb:

LmjF.35.5210 , LMJLV39_350060300 , LMJSD75_350059600

Length:

464

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005739	mitochondrion	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9AFZ4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFZ4

PDB structure(s): 7am2_BT

Function

Biological processes
Term	Name	Level	Count
GO:0006412	translation	4	2
GO:0006518	peptide metabolic process	4	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0009058	biosynthetic process	2	2
GO:0009059	macromolecule biosynthetic process	4	2
GO:0009987	cellular process	1	2
GO:0019538	protein metabolic process	3	2
GO:0032543	mitochondrial translation	5	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0034645	obsolete cellular macromolecule biosynthetic process	4	2
GO:0043043	peptide biosynthetic process	5	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043603	amide metabolic process	3	2
GO:0043604	amide biosynthetic process	4	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044249	cellular biosynthetic process	3	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0044271	cellular nitrogen compound biosynthetic process	4	2
GO:0071704	organic substance metabolic process	2	2
GO:1901564	organonitrogen compound metabolic process	3	2
GO:1901566	organonitrogen compound biosynthetic process	4	2
GO:1901576	organic substance biosynthetic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003824	catalytic activity	1	2
GO:0003924	GTPase activity	7	2
GO:0005488	binding	1	12
GO:0005525	GTP binding	5	12
GO:0016462	pyrophosphatase activity	5	2
GO:0016787	hydrolase activity	2	2
GO:0016817	hydrolase activity, acting on acid anhydrides	3	2
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4	2
GO:0017076	purine nucleotide binding	4	12
GO:0017111	ribonucleoside triphosphate phosphatase activity	6	2
GO:0019001	guanyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032561	guanyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	77	81	PF00656	0.282
CLV_NRD_NRD_1	136	138	PF00675	0.535
CLV_NRD_NRD_1	16	18	PF00675	0.448
CLV_NRD_NRD_1	197	199	PF00675	0.218
CLV_NRD_NRD_1	346	348	PF00675	0.458
CLV_NRD_NRD_1	418	420	PF00675	0.667
CLV_NRD_NRD_1	438	440	PF00675	0.467
CLV_NRD_NRD_1	92	94	PF00675	0.388
CLV_PCSK_KEX2_1	136	138	PF00082	0.401
CLV_PCSK_KEX2_1	197	199	PF00082	0.218
CLV_PCSK_KEX2_1	20	22	PF00082	0.453
CLV_PCSK_KEX2_1	346	348	PF00082	0.437
CLV_PCSK_KEX2_1	418	420	PF00082	0.604
CLV_PCSK_KEX2_1	455	457	PF00082	0.584
CLV_PCSK_PC1ET2_1	20	22	PF00082	0.415
CLV_PCSK_PC1ET2_1	455	457	PF00082	0.616
CLV_PCSK_PC7_1	193	199	PF00082	0.218
CLV_PCSK_SKI1_1	17	21	PF00082	0.415
CLV_PCSK_SKI1_1	213	217	PF00082	0.218
CLV_PCSK_SKI1_1	346	350	PF00082	0.493
CLV_PCSK_SKI1_1	40	44	PF00082	0.466
CLV_PCSK_SKI1_1	401	405	PF00082	0.595
CLV_PCSK_SKI1_1	93	97	PF00082	0.513
DEG_APCC_DBOX_1	400	408	PF00400	0.554
DEG_Nend_UBRbox_1	1	4	PF02207	0.540
DEG_SCF_FBW7_1	205	212	PF00400	0.439
DEG_SCF_FBW7_1	228	235	PF00400	0.514
DOC_CKS1_1	229	234	PF01111	0.518
DOC_CYCLIN_RxL_1	343	352	PF00134	0.343
DOC_CYCLIN_yCln2_LP_2	407	413	PF00134	0.592
DOC_MAPK_gen_1	197	207	PF00069	0.408
DOC_MAPK_MEF2A_6	160	169	PF00069	0.334
DOC_PP1_RVXF_1	99	106	PF00149	0.323
DOC_PP2B_LxvP_1	234	237	PF13499	0.493
DOC_PP2B_LxvP_1	287	290	PF13499	0.339
DOC_SPAK_OSR1_1	325	329	PF12202	0.456
DOC_USP7_MATH_1	276	280	PF00917	0.454
DOC_USP7_MATH_1	34	38	PF00917	0.604
DOC_WW_Pin1_4	198	203	PF00397	0.426
DOC_WW_Pin1_4	205	210	PF00397	0.435
DOC_WW_Pin1_4	228	233	PF00397	0.426
DOC_WW_Pin1_4	427	432	PF00397	0.718
DOC_WW_Pin1_4	458	463	PF00397	0.481
LIG_14-3-3_CanoR_1	148	157	PF00244	0.351
LIG_14-3-3_CanoR_1	2	6	PF00244	0.604
LIG_14-3-3_CanoR_1	21	30	PF00244	0.332
LIG_14-3-3_CanoR_1	291	297	PF00244	0.316
LIG_14-3-3_CanoR_1	347	357	PF00244	0.507
LIG_14-3-3_CanoR_1	446	454	PF00244	0.568
LIG_14-3-3_CanoR_1	82	92	PF00244	0.328
LIG_14-3-3_CanoR_1	93	102	PF00244	0.351
LIG_Actin_WH2_2	336	352	PF00022	0.512
LIG_APCC_ABBAyCdc20_2	408	414	PF00400	0.604
LIG_eIF4E_1	269	275	PF01652	0.351
LIG_EVH1_1	154	158	PF00568	0.416
LIG_FHA_1	148	154	PF00498	0.343
LIG_FHA_1	202	208	PF00498	0.428
LIG_FHA_1	229	235	PF00498	0.503
LIG_FHA_1	254	260	PF00498	0.372
LIG_FHA_1	338	344	PF00498	0.465
LIG_FHA_1	382	388	PF00498	0.534
LIG_FHA_2	446	452	PF00498	0.683
LIG_GBD_Chelix_1	266	274	PF00786	0.295
LIG_LIR_Gen_1	41	51	PF02991	0.452
LIG_LIR_Nem_3	41	46	PF02991	0.470
LIG_PCNA_yPIPBox_3	116	125	PF02747	0.403
LIG_Pex14_2	11	15	PF04695	0.467
LIG_Pex14_2	327	331	PF04695	0.385
LIG_SH2_CRK	159	163	PF00017	0.528
LIG_SH2_CRK	229	233	PF00017	0.407
LIG_SH2_CRK	271	275	PF00017	0.304
LIG_SH2_NCK_1	229	233	PF00017	0.435
LIG_SH2_STAP1	271	275	PF00017	0.384
LIG_SH2_STAT5	269	272	PF00017	0.276
LIG_SH2_STAT5	54	57	PF00017	0.417
LIG_SH3_2	155	160	PF14604	0.517
LIG_SH3_3	152	158	PF00018	0.515
LIG_SH3_3	166	172	PF00018	0.277
LIG_SH3_3	256	262	PF00018	0.526
LIG_SH3_3	287	293	PF00018	0.326
LIG_SH3_3	7	13	PF00018	0.451
LIG_SUMO_SIM_anti_2	340	345	PF11976	0.476
LIG_SUMO_SIM_par_1	203	208	PF11976	0.458
LIG_SUMO_SIM_par_1	223	231	PF11976	0.352
LIG_SUMO_SIM_par_1	73	80	PF11976	0.292
LIG_TRAF2_1	118	121	PF00917	0.443
LIG_TYR_ITIM	157	162	PF00017	0.433
LIG_UBA3_1	153	160	PF00899	0.425
LIG_UBA3_1	332	338	PF00899	0.251
LIG_WW_2	155	158	PF00397	0.429
MOD_CDK_SPxK_1	458	464	PF00069	0.484
MOD_CK1_1	201	207	PF00069	0.528
MOD_CK1_1	277	283	PF00069	0.476
MOD_CK1_1	29	35	PF00069	0.543
MOD_CK1_1	307	313	PF00069	0.393
MOD_CK1_1	351	357	PF00069	0.686
MOD_CK1_1	361	367	PF00069	0.608
MOD_CK1_1	432	438	PF00069	0.640
MOD_CK1_1	445	451	PF00069	0.501
MOD_DYRK1A_RPxSP_1	198	202	PF00069	0.431
MOD_GlcNHglycan	133	136	PF01048	0.422
MOD_GlcNHglycan	23	26	PF01048	0.539
MOD_GlcNHglycan	243	246	PF01048	0.474
MOD_GlcNHglycan	276	279	PF01048	0.402
MOD_GlcNHglycan	305	309	PF01048	0.381
MOD_GlcNHglycan	31	35	PF01048	0.512
MOD_GlcNHglycan	360	363	PF01048	0.537
MOD_GlcNHglycan	59	62	PF01048	0.460
MOD_GSK3_1	108	115	PF00069	0.478
MOD_GSK3_1	175	182	PF00069	0.408
MOD_GSK3_1	201	208	PF00069	0.454
MOD_GSK3_1	228	235	PF00069	0.418
MOD_GSK3_1	253	260	PF00069	0.363
MOD_GSK3_1	26	33	PF00069	0.506
MOD_GSK3_1	306	313	PF00069	0.404
MOD_GSK3_1	348	355	PF00069	0.579
MOD_GSK3_1	358	365	PF00069	0.543
MOD_NEK2_1	1	6	PF00069	0.632
MOD_NEK2_1	131	136	PF00069	0.392
MOD_NEK2_1	179	184	PF00069	0.413
MOD_NEK2_1	196	201	PF00069	0.463
MOD_NEK2_1	274	279	PF00069	0.460
MOD_NEK2_1	30	35	PF00069	0.507
MOD_NEK2_1	348	353	PF00069	0.480
MOD_NEK2_1	358	363	PF00069	0.577
MOD_PIKK_1	349	355	PF00454	0.517
MOD_PKA_1	455	461	PF00069	0.633
MOD_PKA_1	93	99	PF00069	0.513
MOD_PKA_2	1	7	PF00069	0.629
MOD_PKA_2	147	153	PF00069	0.414
MOD_PKA_2	196	202	PF00069	0.474
MOD_PKA_2	349	355	PF00069	0.431
MOD_PKA_2	445	451	PF00069	0.580
MOD_PKA_2	455	461	PF00069	0.517
MOD_Plk_1	257	263	PF00069	0.365
MOD_Plk_1	432	438	PF00069	0.556
MOD_Plk_4	175	181	PF00069	0.407
MOD_Plk_4	432	438	PF00069	0.556
MOD_ProDKin_1	198	204	PF00069	0.426
MOD_ProDKin_1	205	211	PF00069	0.435
MOD_ProDKin_1	228	234	PF00069	0.426
MOD_ProDKin_1	427	433	PF00069	0.719
MOD_ProDKin_1	458	464	PF00069	0.484
MOD_SUMO_rev_2	41	51	PF00179	0.546
TRG_DiLeu_BaLyEn_6	344	349	PF01217	0.569
TRG_DiLeu_BaLyEn_6	354	359	PF01217	0.668
TRG_DiLeu_BaLyEn_6	98	103	PF01217	0.342
TRG_ENDOCYTIC_2	159	162	PF00928	0.541
TRG_ENDOCYTIC_2	271	274	PF00928	0.295
TRG_ER_diArg_1	196	198	PF00400	0.418
TRG_ER_diArg_1	345	347	PF00400	0.470
TRG_ER_diArg_1	417	419	PF00400	0.600
TRG_NLS_MonoExtC_3	16	21	PF00514	0.530
TRG_NLS_MonoExtC_3	438	443	PF00514	0.563
TRG_Pf-PMV_PEXEL_1	117	121	PF00026	0.360
TRG_Pf-PMV_PEXEL_1	268	272	PF00026	0.284

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IKR9	Leptomonas seymouri	84%	100%
A0A0S4JQF8	Bodo saltans	54%	100%
A0A1X0P5K5	Trypanosomatidae	67%	100%
A0A3R7M7F0	Trypanosoma rangeli	67%	100%
A0A3S7XAB8	Leishmania donovani	96%	100%
A4IC39	Leishmania infantum	95%	100%
C9ZYD4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	65%	100%
E3TDS3	Ictalurus punctatus	26%	100%
E9B747	Leishmania mexicana (strain MHOM/GT/2001/U1103)	96%	100%
O15827	Leishmania braziliensis	91%	100%
O74776	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	24%	100%
P53145	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	25%	72%
Q29AU5	Drosophila pseudoobscura pseudoobscura	28%	100%
Q8R2R6	Mus musculus	29%	100%
Q9BT17	Homo sapiens	29%	100%
Q9VCU5	Drosophila melanogaster	27%	100%
V5BTY1	Trypanosoma cruzi	65%	100%