Putative folate/biopterin transporter

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

NIMA-related kinase, putative

Species:

Leishmania major

UniProt:

E9AFZ2_LEIMA

TriTrypDb:

LmjF.35.5190 , LMJLV39_350060000 , LMJSD75_350059300

Length:

477

Annotations

LeishMANIAdb annotations

A large and apprently artificial collection of diverse kinetoplastid protein kinases. None of them appear to be TM.

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 2
Forrest at al. (procyclic)	no	yes: 2
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 33

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 10

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 74
NetGPI	no	yes: 0, no: 74

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1
GO:0097014	ciliary plasm	5	1
GO:0099568	cytoplasmic region	3	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AFZ2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFZ2

Function

Biological processes
Term	Name	Level	Count
GO:0006468	protein phosphorylation	5	75
GO:0006793	phosphorus metabolic process	3	75
GO:0006796	phosphate-containing compound metabolic process	4	75
GO:0006807	nitrogen compound metabolic process	2	75
GO:0008152	metabolic process	1	75
GO:0009987	cellular process	1	75
GO:0016310	phosphorylation	5	75
GO:0019538	protein metabolic process	3	75
GO:0036211	protein modification process	4	75
GO:0043170	macromolecule metabolic process	3	75
GO:0043412	macromolecule modification	4	75
GO:0044237	cellular metabolic process	2	75
GO:0044238	primary metabolic process	2	75
GO:0071704	organic substance metabolic process	2	75
GO:1901564	organonitrogen compound metabolic process	3	75
GO:0000281	mitotic cytokinesis	4	1
GO:0000910	cytokinesis	3	1
GO:0018105	peptidyl-serine phosphorylation	6	1
GO:0018193	peptidyl-amino acid modification	5	1
GO:0018209	peptidyl-serine modification	6	1
GO:0022402	cell cycle process	2	1
GO:0061640	cytoskeleton-dependent cytokinesis	4	1
GO:1903047	mitotic cell cycle process	3	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	75
GO:0003824	catalytic activity	1	75
GO:0004672	protein kinase activity	3	75
GO:0005488	binding	1	75
GO:0005524	ATP binding	5	75
GO:0016301	kinase activity	4	75
GO:0016740	transferase activity	2	75
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	75
GO:0016773	phosphotransferase activity, alcohol group as acceptor	4	75
GO:0017076	purine nucleotide binding	4	75
GO:0030554	adenyl nucleotide binding	5	75
GO:0032553	ribonucleotide binding	3	75
GO:0032555	purine ribonucleotide binding	4	75
GO:0032559	adenyl ribonucleotide binding	5	75
GO:0035639	purine ribonucleoside triphosphate binding	4	75
GO:0036094	small molecule binding	2	75
GO:0043167	ion binding	2	75
GO:0043168	anion binding	3	75
GO:0097159	organic cyclic compound binding	2	75
GO:0097367	carbohydrate derivative binding	2	75
GO:0140096	catalytic activity, acting on a protein	2	75
GO:1901265	nucleoside phosphate binding	3	75
GO:1901363	heterocyclic compound binding	2	75
GO:0004674	protein serine/threonine kinase activity	4	43

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	187	189	PF00675	0.318
CLV_NRD_NRD_1	243	245	PF00675	0.267
CLV_PCSK_KEX2_1	187	189	PF00082	0.358
CLV_PCSK_KEX2_1	245	247	PF00082	0.285
CLV_PCSK_KEX2_1	393	395	PF00082	0.318
CLV_PCSK_PC1ET2_1	245	247	PF00082	0.337
CLV_PCSK_PC1ET2_1	393	395	PF00082	0.319
CLV_PCSK_SKI1_1	106	110	PF00082	0.433
CLV_PCSK_SKI1_1	138	142	PF00082	0.353
CLV_PCSK_SKI1_1	153	157	PF00082	0.267
CLV_PCSK_SKI1_1	192	196	PF00082	0.298
CLV_PCSK_SKI1_1	268	272	PF00082	0.312
DEG_APCC_DBOX_1	111	119	PF00400	0.416
DEG_COP1_1	424	435	PF00400	0.355
DEG_SPOP_SBC_1	293	297	PF00917	0.163
DOC_CDC14_PxL_1	235	243	PF14671	0.313
DOC_MAPK_gen_1	156	165	PF00069	0.318
DOC_MAPK_gen_1	192	201	PF00069	0.304
DOC_MAPK_MEF2A_6	134	143	PF00069	0.230
DOC_MAPK_MEF2A_6	21	28	PF00069	0.436
DOC_MAPK_MEF2A_6	439	448	PF00069	0.401
DOC_PP4_FxxP_1	200	203	PF00568	0.319
DOC_PP4_FxxP_1	270	273	PF00568	0.321
DOC_PP4_FxxP_1	430	433	PF00568	0.272
DOC_USP7_MATH_1	293	297	PF00917	0.163
DOC_USP7_MATH_1	362	366	PF00917	0.577
DOC_USP7_UBL2_3	134	138	PF12436	0.296
DOC_USP7_UBL2_3	156	160	PF12436	0.284
DOC_USP7_UBL2_3	245	249	PF12436	0.256
DOC_USP7_UBL2_3	94	98	PF12436	0.392
DOC_WW_Pin1_4	230	235	PF00397	0.311
DOC_WW_Pin1_4	340	345	PF00397	0.536
DOC_WW_Pin1_4	55	60	PF00397	0.680
LIG_14-3-3_CanoR_1	106	111	PF00244	0.342
LIG_14-3-3_CanoR_1	313	320	PF00244	0.256
LIG_14-3-3_CanoR_1	337	344	PF00244	0.375
LIG_14-3-3_CanoR_1	404	408	PF00244	0.272
LIG_14-3-3_CanoR_1	76	84	PF00244	0.454
LIG_APCC_ABBAyCdc20_2	187	193	PF00400	0.327
LIG_Clathr_ClatBox_1	140	144	PF01394	0.248
LIG_deltaCOP1_diTrp_1	388	396	PF00928	0.312
LIG_deltaCOP1_diTrp_1	465	471	PF00928	0.355
LIG_eIF4E_1	168	174	PF01652	0.354
LIG_eIF4E_1	22	28	PF01652	0.419
LIG_eIF4E_1	68	74	PF01652	0.356
LIG_FHA_1	11	17	PF00498	0.532
LIG_FHA_1	202	208	PF00498	0.258
LIG_FHA_1	314	320	PF00498	0.331
LIG_FHA_1	371	377	PF00498	0.479
LIG_FHA_1	69	75	PF00498	0.446
LIG_FHA_2	27	33	PF00498	0.349
LIG_FHA_2	423	429	PF00498	0.334
LIG_FHA_2	458	464	PF00498	0.315
LIG_FHA_2	46	52	PF00498	0.432
LIG_HCF-1_HBM_1	65	68	PF13415	0.512
LIG_LIR_Apic_2	233	239	PF02991	0.301
LIG_LIR_Apic_2	428	433	PF02991	0.296
LIG_LIR_Nem_3	322	327	PF02991	0.358
LIG_NRBOX	259	265	PF00104	0.263
LIG_Pex14_1	391	395	PF04695	0.295
LIG_Pex14_1	467	471	PF04695	0.353
LIG_SH2_CRK	175	179	PF00017	0.355
LIG_SH2_GRB2like	457	460	PF00017	0.156
LIG_SH2_SRC	175	178	PF00017	0.331
LIG_SH2_STAP1	19	23	PF00017	0.387
LIG_SH2_STAP1	218	222	PF00017	0.281
LIG_SH2_STAP1	247	251	PF00017	0.340
LIG_SH2_STAP1	457	461	PF00017	0.301
LIG_SH2_STAT5	22	25	PF00017	0.433
LIG_SH2_STAT5	236	239	PF00017	0.298
LIG_SH2_STAT5	327	330	PF00017	0.388
LIG_SH2_STAT5	381	384	PF00017	0.336
LIG_SH2_STAT5	395	398	PF00017	0.289
LIG_SH2_STAT5	68	71	PF00017	0.368
LIG_SH3_3	47	53	PF00018	0.523
LIG_SUMO_SIM_anti_2	135	144	PF11976	0.277
LIG_SUMO_SIM_par_1	135	144	PF11976	0.317
LIG_SUMO_SIM_par_1	177	183	PF11976	0.247
LIG_TRAF2_1	164	167	PF00917	0.400
LIG_TRAF2_1	29	32	PF00917	0.357
LIG_TRFH_1	235	239	PF08558	0.276
LIG_TYR_ITIM	173	178	PF00017	0.324
LIG_TYR_ITIM	259	264	PF00017	0.317
LIG_UBA3_1	263	271	PF00899	0.304
LIG_UBA3_1	301	308	PF00899	0.324
MOD_CDK_SPxxK_3	340	347	PF00069	0.295
MOD_CK1_1	10	16	PF00069	0.471
MOD_CK1_1	374	380	PF00069	0.302
MOD_CK1_1	58	64	PF00069	0.575
MOD_CK1_1	89	95	PF00069	0.292
MOD_CK2_1	26	32	PF00069	0.385
MOD_CK2_1	271	277	PF00069	0.398
MOD_CK2_1	403	409	PF00069	0.306
MOD_GlcNHglycan	146	149	PF01048	0.287
MOD_GlcNHglycan	195	198	PF01048	0.340
MOD_GlcNHglycan	356	359	PF01048	0.598
MOD_GlcNHglycan	9	12	PF01048	0.589
MOD_GSK3_1	106	113	PF00069	0.334
MOD_GSK3_1	212	219	PF00069	0.283
MOD_GSK3_1	226	233	PF00069	0.299
MOD_GSK3_1	304	311	PF00069	0.345
MOD_GSK3_1	336	343	PF00069	0.460
MOD_GSK3_1	370	377	PF00069	0.553
MOD_NEK2_1	212	217	PF00069	0.297
MOD_NEK2_1	403	408	PF00069	0.287
MOD_NEK2_1	466	471	PF00069	0.304
MOD_NEK2_1	83	88	PF00069	0.425
MOD_PIKK_1	27	33	PF00454	0.338
MOD_PIKK_1	271	277	PF00454	0.319
MOD_PIKK_1	313	319	PF00454	0.271
MOD_PIKK_1	371	377	PF00454	0.570
MOD_PIKK_1	434	440	PF00454	0.401
MOD_PKA_1	245	251	PF00069	0.328
MOD_PKA_2	245	251	PF00069	0.323
MOD_PKA_2	312	318	PF00069	0.293
MOD_PKA_2	336	342	PF00069	0.388
MOD_PKA_2	403	409	PF00069	0.319
MOD_PKA_2	86	92	PF00069	0.417
MOD_Plk_2-3	132	138	PF00069	0.296
MOD_Plk_2-3	220	226	PF00069	0.319
MOD_Plk_4	69	75	PF00069	0.466
MOD_ProDKin_1	230	236	PF00069	0.311
MOD_ProDKin_1	340	346	PF00069	0.534
MOD_ProDKin_1	55	61	PF00069	0.668
MOD_SUMO_rev_2	131	140	PF00179	0.348
TRG_DiLeu_BaLyEn_6	301	306	PF01217	0.374
TRG_ENDOCYTIC_2	175	178	PF00928	0.326
TRG_ENDOCYTIC_2	261	264	PF00928	0.304
TRG_ENDOCYTIC_2	369	372	PF00928	0.536
TRG_ENDOCYTIC_2	395	398	PF00928	0.326
TRG_ER_diArg_1	187	189	PF00400	0.347
TRG_ER_diArg_1	244	247	PF00400	0.293
TRG_NLS_MonoExtN_4	390	396	PF00514	0.320
TRG_Pf-PMV_PEXEL_1	276	280	PF00026	0.300
TRG_Pf-PMV_PEXEL_1	352	356	PF00026	0.478

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P3N5	Leptomonas seymouri	38%	100%
A0A0N0P953	Leptomonas seymouri	88%	100%
A0A0N1I9A0	Leptomonas seymouri	28%	73%
A0A0N1PD05	Leptomonas seymouri	36%	100%
A0A0S4IMB7	Bodo saltans	38%	100%
A0A0S4IQ75	Bodo saltans	30%	95%
A0A0S4IRZ7	Bodo saltans	35%	97%
A0A0S4IVW1	Bodo saltans	28%	76%
A0A0S4IX86	Bodo saltans	39%	100%
A0A0S4J804	Bodo saltans	64%	91%
A0A0S4JIJ6	Bodo saltans	30%	84%
A0A0S4JPZ1	Bodo saltans	34%	100%
A0A1X0NIA0	Trypanosomatidae	38%	100%
A0A1X0NIX2	Trypanosomatidae	34%	100%
A0A1X0NUB2	Trypanosomatidae	32%	82%
A0A1X0P527	Trypanosomatidae	38%	100%
A0A1X0P549	Trypanosomatidae	75%	100%
A0A1X0P863	Trypanosomatidae	35%	96%
A0A1X0P994	Trypanosomatidae	34%	96%
A0A3Q8IAQ1	Leishmania donovani	43%	100%
A0A3Q8IFW0	Leishmania donovani	38%	100%
A0A3Q8IIG1	Leishmania donovani	31%	100%
A0A3Q8IJM9	Leishmania donovani	97%	100%
A0A3R7KCZ4	Trypanosoma rangeli	40%	100%
A0A3R7MKG5	Trypanosoma rangeli	36%	100%
A0A3S7WYP4	Leishmania donovani	27%	100%
A0A3S7X2W9	Leishmania donovani	25%	100%
A0A3S7X5M4	Leishmania donovani	29%	73%
A0A3S7X6T8	Leishmania donovani	35%	100%
A0A3S7XAL3	Leishmania donovani	35%	96%
A0A3S7XAT9	Leishmania donovani	35%	95%
A0A422NCP0	Trypanosoma rangeli	35%	96%
A0A422NH41	Trypanosoma rangeli	33%	83%
A4H4S9	Leishmania braziliensis	25%	100%
A4HH03	Leishmania braziliensis	28%	100%
A4HHS9	Leishmania braziliensis	24%	100%
A4HJW2	Leishmania braziliensis	37%	100%
A4HKG9	Leishmania braziliensis	30%	73%
A4HLR0	Leishmania braziliensis	35%	100%
A4HNI1	Leishmania braziliensis	90%	100%
A4HP12	Leishmania braziliensis	35%	94%
A4HP13	Leishmania braziliensis	35%	95%
A4HZW8	Leishmania infantum	43%	100%
A4I140	Leishmania infantum	27%	100%
A4I435	Leishmania infantum	31%	100%
A4I4Y0	Leishmania infantum	25%	100%
A4I7C4	Leishmania infantum	38%	100%
A4I7Z6	Leishmania infantum	29%	73%
A4I960	Leishmania infantum	35%	100%
A4IC37	Leishmania infantum	97%	100%
A4IDC1	Leishmania infantum	34%	94%
A4IDC2	Leishmania infantum	35%	95%
C9ZMH9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	35%	100%
C9ZWI1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	39%	100%
C9ZWK2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	38%	100%
D0A2Z1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	36%	97%
D0A2Z6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	36%	97%
D0AA64	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	34%	83%
E9AED4	Leishmania major	26%	100%
E9ASS2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	94%
E9ASS3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	36%	95%
E9AVS7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	39%	100%
E9B0C2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	32%	100%
E9B2B7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	38%	100%
E9B2V8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	73%
E9B436	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
E9B745	Leishmania mexicana (strain MHOM/GT/2001/U1103)	96%	100%
Q00771	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	27%	100%
Q03428	Trypanosoma brucei brucei	38%	100%
Q08942	Trypanosoma brucei brucei	39%	100%
Q4Q1S3	Leishmania major	37%	95%
Q4Q1S4	Leishmania major	34%	94%
Q4Q3Y9	Leishmania major	35%	100%
Q4Q598	Leishmania major	30%	73%
Q4Q5T9	Leishmania major	38%	100%
Q4Q7W2	Leishmania major	31%	100%
Q4Q9K2	Leishmania major	31%	100%
Q4QBQ2	Leishmania major	40%	100%
Q4QCK0	Leishmania major	23%	100%
Q4QF23	Leishmania major	27%	100%
V5BC28	Trypanosoma cruzi	38%	95%
V5BPJ0	Trypanosoma cruzi	35%	96%
V5D579	Trypanosoma cruzi	39%	100%
V5DFW9	Trypanosoma cruzi	32%	82%
V5DKY9	Trypanosoma cruzi	28%	77%