PseudoU_synth_2 domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

RNA pseudouridylate synthase, putative

Species:

Leishmania major

UniProt:

E9AFY4_LEIMA

TriTrypDb:

LmjF.35.5110 , LMJLV39_350059200 , LMJSD75_350058500

Length:

741

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

E9AFY4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFY4

Function

Biological processes
Term	Name	Level	Count
GO:0000154	rRNA modification	6	2
GO:0000455	enzyme-directed rRNA pseudouridine synthesis	8	2
GO:0001522	pseudouridine synthesis	6	12
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006364	rRNA processing	8	2
GO:0006396	RNA processing	6	2
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009451	RNA modification	5	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0016072	rRNA metabolic process	7	2
GO:0031118	rRNA pseudouridine synthesis	7	2
GO:0034470	ncRNA processing	7	2
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034660	ncRNA metabolic process	6	2
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	12
GO:0009982	pseudouridine synthase activity	4	12
GO:0016853	isomerase activity	2	12
GO:0016866	intramolecular transferase activity	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:1901363	heterocyclic compound binding	2	12
GO:0009055	electron transfer activity	3	1
GO:0016491	oxidoreductase activity	2	1
GO:0020037	heme binding	4	1
GO:0043167	ion binding	2	1
GO:0043169	cation binding	3	1
GO:0046872	metal ion binding	4	1
GO:0046906	tetrapyrrole binding	3	1
GO:0106029	tRNA pseudouridine synthase activity	5	1
GO:0140098	catalytic activity, acting on RNA	3	1
GO:0140101	catalytic activity, acting on a tRNA	4	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	235	239	PF00656	0.614
CLV_C14_Caspase3-7	631	635	PF00656	0.441
CLV_NRD_NRD_1	12	14	PF00675	0.418
CLV_NRD_NRD_1	128	130	PF00675	0.394
CLV_NRD_NRD_1	249	251	PF00675	0.631
CLV_NRD_NRD_1	331	333	PF00675	0.488
CLV_NRD_NRD_1	336	338	PF00675	0.495
CLV_NRD_NRD_1	671	673	PF00675	0.231
CLV_NRD_NRD_1	729	731	PF00675	0.392
CLV_PCSK_FUR_1	247	251	PF00082	0.491
CLV_PCSK_FUR_1	329	333	PF00082	0.523
CLV_PCSK_KEX2_1	128	130	PF00082	0.386
CLV_PCSK_KEX2_1	249	251	PF00082	0.518
CLV_PCSK_KEX2_1	331	333	PF00082	0.484
CLV_PCSK_KEX2_1	336	338	PF00082	0.491
CLV_PCSK_KEX2_1	387	389	PF00082	0.420
CLV_PCSK_KEX2_1	671	673	PF00082	0.236
CLV_PCSK_KEX2_1	729	731	PF00082	0.427
CLV_PCSK_PC1ET2_1	387	389	PF00082	0.420
CLV_PCSK_PC7_1	332	338	PF00082	0.511
CLV_PCSK_SKI1_1	101	105	PF00082	0.521
CLV_PCSK_SKI1_1	13	17	PF00082	0.492
CLV_PCSK_SKI1_1	283	287	PF00082	0.549
CLV_PCSK_SKI1_1	357	361	PF00082	0.486
CLV_PCSK_SKI1_1	384	388	PF00082	0.457
CLV_PCSK_SKI1_1	465	469	PF00082	0.336
CLV_PCSK_SKI1_1	564	568	PF00082	0.236
CLV_PCSK_SKI1_1	57	61	PF00082	0.545
CLV_PCSK_SKI1_1	699	703	PF00082	0.263
DEG_APCC_DBOX_1	12	20	PF00400	0.471
DEG_APCC_DBOX_1	33	41	PF00400	0.444
DEG_APCC_DBOX_1	437	445	PF00400	0.403
DEG_APCC_DBOX_1	464	472	PF00400	0.364
DEG_APCC_DBOX_1	728	736	PF00400	0.459
DEG_SCF_FBW7_1	78	84	PF00400	0.462
DEG_SPOP_SBC_1	262	266	PF00917	0.507
DEG_SPOP_SBC_1	82	86	PF00917	0.533
DEG_SPOP_SBC_1	89	93	PF00917	0.545
DOC_CDC14_PxL_1	509	517	PF14671	0.479
DOC_CKS1_1	213	218	PF01111	0.476
DOC_CKS1_1	542	547	PF01111	0.415
DOC_CKS1_1	78	83	PF01111	0.451
DOC_CYCLIN_RxL_1	460	469	PF00134	0.341
DOC_CYCLIN_RxL_1	727	738	PF00134	0.329
DOC_MAPK_gen_1	13	21	PF00069	0.446
DOC_MAPK_gen_1	641	649	PF00069	0.466
DOC_MAPK_MEF2A_6	610	619	PF00069	0.519
DOC_MAPK_RevD_3	323	337	PF00069	0.607
DOC_PP1_RVXF_1	643	650	PF00149	0.424
DOC_PP1_RVXF_1	652	658	PF00149	0.424
DOC_PP4_FxxP_1	310	313	PF00568	0.545
DOC_PP4_FxxP_1	709	712	PF00568	0.372
DOC_USP7_MATH_1	201	205	PF00917	0.518
DOC_USP7_MATH_1	206	210	PF00917	0.496
DOC_USP7_MATH_1	236	240	PF00917	0.473
DOC_USP7_MATH_1	262	266	PF00917	0.665
DOC_USP7_MATH_1	339	343	PF00917	0.683
DOC_USP7_MATH_1	359	363	PF00917	0.453
DOC_USP7_MATH_1	459	463	PF00917	0.354
DOC_USP7_MATH_1	577	581	PF00917	0.437
DOC_USP7_MATH_1	591	595	PF00917	0.394
DOC_USP7_MATH_1	89	93	PF00917	0.733
DOC_WW_Pin1_4	157	162	PF00397	0.522
DOC_WW_Pin1_4	212	217	PF00397	0.483
DOC_WW_Pin1_4	309	314	PF00397	0.559
DOC_WW_Pin1_4	406	411	PF00397	0.508
DOC_WW_Pin1_4	541	546	PF00397	0.552
DOC_WW_Pin1_4	60	65	PF00397	0.528
DOC_WW_Pin1_4	77	82	PF00397	0.420
LIG_14-3-3_CanoR_1	140	146	PF00244	0.386
LIG_14-3-3_CanoR_1	261	269	PF00244	0.599
LIG_14-3-3_CanoR_1	394	400	PF00244	0.586
LIG_14-3-3_CanoR_1	438	442	PF00244	0.552
LIG_14-3-3_CanoR_1	44	50	PF00244	0.541
LIG_14-3-3_CanoR_1	477	482	PF00244	0.558
LIG_14-3-3_CanoR_1	534	542	PF00244	0.424
LIG_14-3-3_CanoR_1	729	735	PF00244	0.314
LIG_Actin_RPEL_3	37	56	PF02755	0.377
LIG_Actin_WH2_2	341	359	PF00022	0.576
LIG_Actin_WH2_2	43	59	PF00022	0.306
LIG_Actin_WH2_2	608	626	PF00022	0.462
LIG_APCC_ABBA_1	626	631	PF00400	0.441
LIG_BRCT_BRCA1_1	601	605	PF00533	0.519
LIG_BRCT_BRCA1_1	675	679	PF00533	0.471
LIG_BRCT_BRCA1_1	705	709	PF00533	0.437
LIG_deltaCOP1_diTrp_1	352	358	PF00928	0.566
LIG_EH1_1	1	9	PF00400	0.407
LIG_EH1_1	275	283	PF00400	0.521
LIG_FHA_1	183	189	PF00498	0.512
LIG_FHA_1	22	28	PF00498	0.446
LIG_FHA_1	544	550	PF00498	0.428
LIG_FHA_2	120	126	PF00498	0.406
LIG_FHA_2	345	351	PF00498	0.466
LIG_FHA_2	464	470	PF00498	0.452
LIG_FHA_2	61	67	PF00498	0.374
LIG_FHA_2	91	97	PF00498	0.539
LIG_GBD_Chelix_1	244	252	PF00786	0.438
LIG_Integrin_RGD_1	405	407	PF01839	0.545
LIG_LIR_Apic_2	706	712	PF02991	0.365
LIG_LIR_Gen_1	352	360	PF02991	0.569
LIG_LIR_Gen_1	609	619	PF02991	0.436
LIG_LIR_LC3C_4	519	524	PF02991	0.369
LIG_LIR_Nem_3	352	358	PF02991	0.566
LIG_LIR_Nem_3	412	418	PF02991	0.334
LIG_LIR_Nem_3	462	467	PF02991	0.332
LIG_LIR_Nem_3	609	615	PF02991	0.428
LIG_LIR_Nem_3	642	647	PF02991	0.416
LIG_LIR_Nem_3	676	682	PF02991	0.302
LIG_Pex14_1	306	310	PF04695	0.434
LIG_Pex14_2	222	226	PF04695	0.417
LIG_SH2_CRK	612	616	PF00017	0.453
LIG_SH2_CRK	644	648	PF00017	0.424
LIG_SH2_STAP1	399	403	PF00017	0.329
LIG_SH2_STAT5	114	117	PF00017	0.537
LIG_SH2_STAT5	374	377	PF00017	0.542
LIG_SH2_STAT5	418	421	PF00017	0.496
LIG_SH3_3	191	197	PF00018	0.505
LIG_SH3_3	291	297	PF00018	0.530
LIG_SH3_3	487	493	PF00018	0.343
LIG_SH3_3	504	510	PF00018	0.401
LIG_SH3_3	521	527	PF00018	0.492
LIG_SH3_3	615	621	PF00018	0.453
LIG_SUMO_SIM_anti_2	517	525	PF11976	0.279
LIG_SUMO_SIM_anti_2	546	554	PF11976	0.439
LIG_SUMO_SIM_par_1	517	525	PF11976	0.400
LIG_TYR_ITSM	640	647	PF00017	0.273
LIG_UBA3_1	55	60	PF00899	0.405
LIG_WRC_WIRS_1	223	228	PF05994	0.479
MOD_CK1_1	195	201	PF00069	0.381
MOD_CK1_1	232	238	PF00069	0.519
MOD_CK1_1	239	245	PF00069	0.461
MOD_CK1_1	257	263	PF00069	0.644
MOD_CK1_1	302	308	PF00069	0.563
MOD_CK1_1	344	350	PF00069	0.563
MOD_CK1_1	606	612	PF00069	0.268
MOD_CK1_1	695	701	PF00069	0.430
MOD_CK2_1	119	125	PF00069	0.381
MOD_CK2_1	346	352	PF00069	0.442
MOD_CK2_1	60	66	PF00069	0.510
MOD_Cter_Amidation	11	14	PF01082	0.512
MOD_Cter_Amidation	664	667	PF01082	0.242
MOD_GlcNHglycan	199	202	PF01048	0.375
MOD_GlcNHglycan	203	206	PF01048	0.365
MOD_GlcNHglycan	230	234	PF01048	0.493
MOD_GlcNHglycan	265	268	PF01048	0.647
MOD_GlcNHglycan	361	365	PF01048	0.558
MOD_GlcNHglycan	367	370	PF01048	0.561
MOD_GlcNHglycan	375	378	PF01048	0.420
MOD_GlcNHglycan	400	403	PF01048	0.532
MOD_GlcNHglycan	578	582	PF01048	0.252
MOD_GlcNHglycan	601	604	PF01048	0.242
MOD_GlcNHglycan	85	88	PF01048	0.615
MOD_GSK3_1	175	182	PF00069	0.465
MOD_GSK3_1	197	204	PF00069	0.365
MOD_GSK3_1	232	239	PF00069	0.587
MOD_GSK3_1	257	264	PF00069	0.586
MOD_GSK3_1	428	435	PF00069	0.537
MOD_GSK3_1	459	466	PF00069	0.346
MOD_GSK3_1	537	544	PF00069	0.303
MOD_GSK3_1	599	606	PF00069	0.266
MOD_GSK3_1	77	84	PF00069	0.456
MOD_LATS_1	138	144	PF00433	0.252
MOD_N-GLC_1	257	262	PF02516	0.700
MOD_NEK2_1	175	180	PF00069	0.423
MOD_NEK2_1	222	227	PF00069	0.500
MOD_NEK2_1	360	365	PF00069	0.593
MOD_NEK2_1	395	400	PF00069	0.371
MOD_NEK2_1	411	416	PF00069	0.492
MOD_NEK2_1	439	444	PF00069	0.386
MOD_NEK2_1	471	476	PF00069	0.388
MOD_NEK2_1	530	535	PF00069	0.256
MOD_NEK2_1	537	542	PF00069	0.226
MOD_NEK2_1	599	604	PF00069	0.233
MOD_NEK2_1	735	740	PF00069	0.439
MOD_NEK2_2	591	596	PF00069	0.242
MOD_PIKK_1	239	245	PF00454	0.425
MOD_PIKK_1	537	543	PF00454	0.242
MOD_PKA_2	119	125	PF00069	0.372
MOD_PKA_2	432	438	PF00069	0.513
MOD_PKA_2	476	482	PF00069	0.605
MOD_PKA_2	735	741	PF00069	0.403
MOD_Plk_1	283	289	PF00069	0.530
MOD_Plk_1	411	417	PF00069	0.489
MOD_Plk_4	206	212	PF00069	0.425
MOD_Plk_4	341	347	PF00069	0.574
MOD_Plk_4	370	376	PF00069	0.602
MOD_Plk_4	45	51	PF00069	0.494
MOD_Plk_4	459	465	PF00069	0.353
MOD_Plk_4	692	698	PF00069	0.461
MOD_Plk_4	705	711	PF00069	0.273
MOD_Plk_4	730	736	PF00069	0.476
MOD_ProDKin_1	157	163	PF00069	0.523
MOD_ProDKin_1	212	218	PF00069	0.479
MOD_ProDKin_1	309	315	PF00069	0.558
MOD_ProDKin_1	406	412	PF00069	0.507
MOD_ProDKin_1	541	547	PF00069	0.435
MOD_ProDKin_1	60	66	PF00069	0.527
MOD_ProDKin_1	77	83	PF00069	0.434
MOD_SUMO_for_1	115	118	PF00179	0.393
MOD_SUMO_for_1	486	489	PF00179	0.367
MOD_SUMO_rev_2	479	488	PF00179	0.550
MOD_SUMO_rev_2	631	640	PF00179	0.268
TRG_DiLeu_BaLyEn_6	391	396	PF01217	0.390
TRG_DiLeu_BaLyEn_6	446	451	PF01217	0.529
TRG_ENDOCYTIC_2	172	175	PF00928	0.316
TRG_ENDOCYTIC_2	612	615	PF00928	0.242
TRG_ENDOCYTIC_2	644	647	PF00928	0.251
TRG_ER_diArg_1	246	249	PF00400	0.458
TRG_ER_diArg_1	328	331	PF00400	0.495
TRG_ER_diArg_1	335	337	PF00400	0.518
TRG_ER_diArg_1	640	643	PF00400	0.463
TRG_ER_diArg_1	670	672	PF00400	0.265
TRG_ER_diArg_1	728	730	PF00400	0.443
TRG_Pf-PMV_PEXEL_1	140	145	PF00026	0.387
TRG_Pf-PMV_PEXEL_1	17	22	PF00026	0.535
TRG_Pf-PMV_PEXEL_1	388	392	PF00026	0.399
TRG_Pf-PMV_PEXEL_1	452	456	PF00026	0.428
TRG_Pf-PMV_PEXEL_1	465	469	PF00026	0.315
TRG_Pf-PMV_PEXEL_1	69	73	PF00026	0.375

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I1F5	Leptomonas seymouri	62%	100%
A0A0S4JPK1	Bodo saltans	36%	100%
A0A1X0NY31	Trypanosomatidae	43%	97%
A0A3Q8IN41	Leishmania donovani	94%	100%
A0A422P4N9	Trypanosoma rangeli	45%	100%
A4HNH3	Leishmania braziliensis	83%	98%
A4IC64	Leishmania infantum	94%	100%
C9ZKA6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	46%	89%
E9B737	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
V5B2G2	Trypanosoma cruzi	45%	100%