PRORP domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania major

UniProt:

E9AFY2_LEIMA

TriTrypDb:

LmjF.35.5090 , LMJLV39_350059000 , LMJSD75_350058300

Length:

584

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 5

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 15
NetGPI	no	yes: 0, no: 15

Cellular components
Term	Name	Level	Count
GO:0005739	mitochondrion	5	12
GO:0043226	organelle	2	16
GO:0043227	membrane-bounded organelle	3	16
GO:0043229	intracellular organelle	3	16
GO:0043231	intracellular membrane-bounded organelle	4	16
GO:0110165	cellular anatomical entity	1	16

Expansion

Sequence features

E9AFY2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFY2

Function

Biological processes
Term	Name	Level	Count
GO:0000966	RNA 5'-end processing	7	2
GO:0001682	tRNA 5'-leader removal	9	2
GO:0006139	nucleobase-containing compound metabolic process	3	16
GO:0006396	RNA processing	6	16
GO:0006399	tRNA metabolic process	7	16
GO:0006725	cellular aromatic compound metabolic process	3	16
GO:0006807	nitrogen compound metabolic process	2	16
GO:0008033	tRNA processing	8	16
GO:0008152	metabolic process	1	16
GO:0009987	cellular process	1	16
GO:0016070	RNA metabolic process	5	16
GO:0034470	ncRNA processing	7	16
GO:0034641	cellular nitrogen compound metabolic process	3	16
GO:0034660	ncRNA metabolic process	6	16
GO:0043170	macromolecule metabolic process	3	16
GO:0044237	cellular metabolic process	2	16
GO:0044238	primary metabolic process	2	16
GO:0046483	heterocycle metabolic process	3	16
GO:0071704	organic substance metabolic process	2	16
GO:0090304	nucleic acid metabolic process	4	16
GO:0099116	tRNA 5'-end processing	8	2
GO:1901360	organic cyclic compound metabolic process	3	16

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	16
GO:0004518	nuclease activity	4	7
GO:0004519	endonuclease activity	5	6
GO:0004521	RNA endonuclease activity	5	6
GO:0004526	ribonuclease P activity	6	6
GO:0004540	RNA nuclease activity	4	6
GO:0004549	tRNA-specific ribonuclease activity	5	6
GO:0016787	hydrolase activity	2	16
GO:0016788	hydrolase activity, acting on ester bonds	3	7
GO:0016891	RNA endonuclease activity, producing 5'-phosphomonoesters	6	6
GO:0016893	endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters	6	6
GO:0140098	catalytic activity, acting on RNA	3	6
GO:0140101	catalytic activity, acting on a tRNA	4	6
GO:0140640	catalytic activity, acting on a nucleic acid	2	6

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	350	354	PF00656	0.452
CLV_C14_Caspase3-7	368	372	PF00656	0.526
CLV_C14_Caspase3-7	467	471	PF00656	0.447
CLV_NRD_NRD_1	164	166	PF00675	0.459
CLV_NRD_NRD_1	177	179	PF00675	0.297
CLV_NRD_NRD_1	21	23	PF00675	0.660
CLV_NRD_NRD_1	414	416	PF00675	0.241
CLV_PCSK_FUR_1	19	23	PF00082	0.715
CLV_PCSK_KEX2_1	164	166	PF00082	0.454
CLV_PCSK_KEX2_1	176	178	PF00082	0.329
CLV_PCSK_KEX2_1	18	20	PF00082	0.678
CLV_PCSK_KEX2_1	21	23	PF00082	0.643
CLV_PCSK_KEX2_1	414	416	PF00082	0.257
CLV_PCSK_PC1ET2_1	176	178	PF00082	0.428
CLV_PCSK_PC1ET2_1	18	20	PF00082	0.728
CLV_PCSK_SKI1_1	164	168	PF00082	0.450
CLV_PCSK_SKI1_1	177	181	PF00082	0.265
CLV_PCSK_SKI1_1	263	267	PF00082	0.475
CLV_PCSK_SKI1_1	296	300	PF00082	0.478
CLV_PCSK_SKI1_1	306	310	PF00082	0.431
CLV_PCSK_SKI1_1	370	374	PF00082	0.380
CLV_PCSK_SKI1_1	512	516	PF00082	0.381
CLV_PCSK_SKI1_1	58	62	PF00082	0.441
CLV_Separin_Metazoa	217	221	PF03568	0.412
CLV_Separin_Metazoa	95	99	PF03568	0.505
DEG_APCC_DBOX_1	163	171	PF00400	0.499
DOC_CKS1_1	109	114	PF01111	0.516
DOC_CKS1_1	430	435	PF01111	0.330
DOC_CYCLIN_RxL_1	260	269	PF00134	0.500
DOC_CYCLIN_yCln2_LP_2	449	455	PF00134	0.373
DOC_MAPK_DCC_7	389	398	PF00069	0.596
DOC_MAPK_gen_1	176	185	PF00069	0.444
DOC_MAPK_gen_1	414	423	PF00069	0.323
DOC_MAPK_gen_1	509	518	PF00069	0.436
DOC_MAPK_HePTP_8	185	197	PF00069	0.410
DOC_MAPK_MEF2A_6	143	151	PF00069	0.380
DOC_MAPK_MEF2A_6	188	197	PF00069	0.417
DOC_MAPK_MEF2A_6	512	520	PF00069	0.452
DOC_MAPK_MEF2A_6	78	85	PF00069	0.508
DOC_PP4_FxxP_1	390	393	PF00568	0.639
DOC_SPAK_OSR1_1	389	393	PF12202	0.624
DOC_SPAK_OSR1_1	490	494	PF12202	0.442
DOC_USP7_MATH_1	2	6	PF00917	0.762
DOC_USP7_MATH_1	30	34	PF00917	0.508
DOC_USP7_MATH_1	344	348	PF00917	0.538
DOC_WW_Pin1_4	108	113	PF00397	0.496
DOC_WW_Pin1_4	289	294	PF00397	0.585
DOC_WW_Pin1_4	429	434	PF00397	0.376
DOC_WW_Pin1_4	448	453	PF00397	0.354
LIG_14-3-3_CanoR_1	389	393	PF00244	0.637
LIG_14-3-3_CanoR_1	498	504	PF00244	0.391
LIG_14-3-3_CanoR_1	512	517	PF00244	0.368
LIG_14-3-3_CanoR_1	522	527	PF00244	0.351
LIG_14-3-3_CanoR_1	6	15	PF00244	0.721
LIG_Actin_WH2_2	137	152	PF00022	0.458
LIG_BIR_III_2	168	172	PF00653	0.486
LIG_BIR_III_4	66	70	PF00653	0.507
LIG_BRCT_BRCA1_1	390	394	PF00533	0.614
LIG_BRCT_BRCA1_1	450	454	PF00533	0.426
LIG_Clathr_ClatBox_1	137	141	PF01394	0.245
LIG_FHA_1	109	115	PF00498	0.526
LIG_FHA_1	238	244	PF00498	0.531
LIG_FHA_1	513	519	PF00498	0.362
LIG_FHA_1	573	579	PF00498	0.547
LIG_FHA_2	230	236	PF00498	0.550
LIG_FHA_2	348	354	PF00498	0.432
LIG_FHA_2	408	414	PF00498	0.453
LIG_FHA_2	465	471	PF00498	0.367
LIG_FHA_2	558	564	PF00498	0.596
LIG_GBD_Chelix_1	299	307	PF00786	0.536
LIG_LIR_Apic_2	108	112	PF02991	0.296
LIG_LIR_Apic_2	388	393	PF02991	0.622
LIG_LIR_Gen_1	212	223	PF02991	0.338
LIG_LIR_Gen_1	335	344	PF02991	0.444
LIG_LIR_Gen_1	347	355	PF02991	0.407
LIG_LIR_Gen_1	457	466	PF02991	0.366
LIG_LIR_Gen_1	481	492	PF02991	0.343
LIG_LIR_Nem_3	212	218	PF02991	0.370
LIG_LIR_Nem_3	335	339	PF02991	0.400
LIG_LIR_Nem_3	347	351	PF02991	0.400
LIG_LIR_Nem_3	457	462	PF02991	0.354
LIG_LIR_Nem_3	481	487	PF02991	0.310
LIG_LIR_Nem_3	502	506	PF02991	0.387
LIG_MAD2	104	112	PF02301	0.489
LIG_PCNA_yPIPBox_3	92	106	PF02747	0.506
LIG_Pex14_1	211	215	PF04695	0.406
LIG_Pex14_1	539	543	PF04695	0.426
LIG_Pex14_2	136	140	PF04695	0.474
LIG_Pex14_2	180	184	PF04695	0.381
LIG_Pex14_2	390	394	PF04695	0.637
LIG_Pex14_2	491	495	PF04695	0.385
LIG_PTB_Apo_2	453	460	PF02174	0.354
LIG_PTB_Apo_2	59	66	PF02174	0.379
LIG_PTB_Phospho_1	453	459	PF10480	0.354
LIG_REV1ctd_RIR_1	489	498	PF16727	0.385
LIG_SH2_CRK	348	352	PF00017	0.409
LIG_SH2_CRK	463	467	PF00017	0.365
LIG_SH2_CRK	503	507	PF00017	0.383
LIG_SH2_STAP1	348	352	PF00017	0.409
LIG_SH2_STAP1	438	442	PF00017	0.417
LIG_SH2_STAP1	524	528	PF00017	0.341
LIG_SH2_STAT5	107	110	PF00017	0.404
LIG_SH2_STAT5	366	369	PF00017	0.554
LIG_SH2_STAT5	459	462	PF00017	0.330
LIG_SH2_STAT5	483	486	PF00017	0.461
LIG_SH2_STAT5	543	546	PF00017	0.452
LIG_SH3_3	176	182	PF00018	0.468
LIG_SH3_3	389	395	PF00018	0.526
LIG_SH3_3	483	489	PF00018	0.471
LIG_SH3_4	309	316	PF00018	0.597
LIG_SUMO_SIM_anti_2	546	551	PF11976	0.531
LIG_TRFH_1	289	293	PF08558	0.432
LIG_TYR_ITIM	357	362	PF00017	0.404
LIG_TYR_ITIM	501	506	PF00017	0.383
LIG_WRC_WIRS_1	286	291	PF05994	0.463
MOD_CDK_SPK_2	108	113	PF00069	0.414
MOD_CDK_SPxxK_3	289	296	PF00069	0.430
MOD_CK1_1	325	331	PF00069	0.564
MOD_CK1_1	33	39	PF00069	0.438
MOD_CK1_1	347	353	PF00069	0.526
MOD_CK1_1	364	370	PF00069	0.455
MOD_CK1_1	383	389	PF00069	0.622
MOD_CK1_1	7	13	PF00069	0.769
MOD_CK2_1	229	235	PF00069	0.562
MOD_CK2_1	313	319	PF00069	0.581
MOD_CK2_1	400	406	PF00069	0.511
MOD_CK2_1	407	413	PF00069	0.424
MOD_Cter_Amidation	415	418	PF01082	0.385
MOD_GlcNHglycan	116	119	PF01048	0.465
MOD_GlcNHglycan	27	31	PF01048	0.577
MOD_GlcNHglycan	385	388	PF01048	0.607
MOD_GlcNHglycan	509	512	PF01048	0.242
MOD_GlcNHglycan	560	563	PF01048	0.609
MOD_GSK3_1	26	33	PF00069	0.547
MOD_GSK3_1	285	292	PF00069	0.467
MOD_GSK3_1	379	386	PF00069	0.577
MOD_GSK3_1	464	471	PF00069	0.398
MOD_GSK3_1	554	561	PF00069	0.596
MOD_GSK3_1	577	584	PF00069	0.569
MOD_N-GLC_1	434	439	PF02516	0.393
MOD_N-GLC_1	507	512	PF02516	0.443
MOD_N-GLC_1	554	559	PF02516	0.661
MOD_NEK2_1	114	119	PF00069	0.476
MOD_NEK2_1	125	130	PF00069	0.424
MOD_NEK2_1	266	271	PF00069	0.538
MOD_NEK2_1	373	378	PF00069	0.537
MOD_NEK2_1	4	9	PF00069	0.767
MOD_PIKK_1	532	538	PF00454	0.464
MOD_PK_1	522	528	PF00069	0.404
MOD_PKA_2	388	394	PF00069	0.636
MOD_PKA_2	499	505	PF00069	0.397
MOD_Plk_1	325	331	PF00069	0.592
MOD_Plk_1	434	440	PF00069	0.437
MOD_Plk_1	554	560	PF00069	0.663
MOD_Plk_4	347	353	PF00069	0.445
MOD_Plk_4	361	367	PF00069	0.383
MOD_Plk_4	37	43	PF00069	0.538
MOD_Plk_4	522	528	PF00069	0.380
MOD_Plk_4	554	560	PF00069	0.586
MOD_Plk_4	81	87	PF00069	0.425
MOD_ProDKin_1	108	114	PF00069	0.488
MOD_ProDKin_1	289	295	PF00069	0.583
MOD_ProDKin_1	429	435	PF00069	0.376
MOD_ProDKin_1	448	454	PF00069	0.354
MOD_SUMO_rev_2	33	41	PF00179	0.460
MOD_SUMO_rev_2	335	342	PF00179	0.532
MOD_SUMO_rev_2	364	372	PF00179	0.459
TRG_DiLeu_BaEn_1	238	243	PF01217	0.543
TRG_DiLeu_BaEn_1	37	42	PF01217	0.541
TRG_ENDOCYTIC_2	215	218	PF00928	0.348
TRG_ENDOCYTIC_2	348	351	PF00928	0.382
TRG_ENDOCYTIC_2	359	362	PF00928	0.386
TRG_ENDOCYTIC_2	459	462	PF00928	0.318
TRG_ENDOCYTIC_2	463	466	PF00928	0.302
TRG_ENDOCYTIC_2	483	486	PF00928	0.461
TRG_ENDOCYTIC_2	503	506	PF00928	0.133
TRG_ER_diArg_1	163	165	PF00400	0.442
TRG_ER_diArg_1	177	179	PF00400	0.264
TRG_ER_diArg_1	19	22	PF00400	0.647
TRG_ER_diArg_1	374	377	PF00400	0.502
TRG_ER_diArg_1	495	498	PF00400	0.324
TRG_NES_CRM1_1	59	74	PF08389	0.298
TRG_NLS_Bipartite_1	164	180	PF00514	0.503
TRG_NLS_MonoCore_2	17	22	PF00514	0.746
TRG_NLS_MonoExtC_3	17	22	PF00514	0.737
TRG_NLS_MonoExtN_4	15	22	PF00514	0.741
TRG_Pf-PMV_PEXEL_1	164	168	PF00026	0.493
TRG_Pf-PMV_PEXEL_1	426	430	PF00026	0.330

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I198	Leptomonas seymouri	67%	99%
A0A0S4JLM5	Bodo saltans	39%	100%
A0A1X0P3X4	Trypanosomatidae	32%	100%
A0A1X0P680	Trypanosomatidae	42%	100%
A0A3Q8IIJ5	Leishmania donovani	94%	100%
A0A3R7LTW4	Trypanosoma rangeli	45%	100%
A0A422MWI6	Trypanosoma rangeli	30%	100%
A4HNH1	Leishmania braziliensis	82%	100%
A4IC62	Leishmania infantum	94%	100%
C9ZYE1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	43%	100%
D0A6B5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	31%	100%
E9B735	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%
V5B8T9	Trypanosoma cruzi	44%	100%
V5BPA7	Trypanosoma cruzi	30%	100%