Polyadenylate-binding protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

polyadenylate-binding protein 1

Species:

Leishmania major

UniProt:

E9AFX7_LEIMA

TriTrypDb:

LmjF.35.5040 , LMJLV39_350058500 , LMJSD75_350057800

Length:

560

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 18

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0005737	cytoplasm	2	10
GO:0005829	cytosol	2	2
GO:0010494	cytoplasmic stress granule	5	2
GO:0032991	protein-containing complex	1	2
GO:0035770	ribonucleoprotein granule	3	2
GO:0036464	cytoplasmic ribonucleoprotein granule	4	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043228	non-membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0043232	intracellular non-membrane-bounded organelle	4	2
GO:0099080	supramolecular complex	2	2
GO:0110165	cellular anatomical entity	1	10
GO:1990904	ribonucleoprotein complex	2	2

Expansion

Sequence features

E9AFX7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFX7

PDB structure(s): 6h7a_A , 6h7a_B , 6h7b_A , 6h7b_C

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006378	mRNA polyadenylation	7	2
GO:0006396	RNA processing	6	2
GO:0006397	mRNA processing	7	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0009987	cellular process	1	2
GO:0016070	RNA metabolic process	5	2
GO:0016071	mRNA metabolic process	6	2
GO:0031123	RNA 3'-end processing	7	2
GO:0031124	mRNA 3'-end processing	8	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043631	RNA polyadenylation	6	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0046483	heterocycle metabolic process	3	2
GO:0071704	organic substance metabolic process	2	2
GO:0090304	nucleic acid metabolic process	4	2
GO:1901360	organic cyclic compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	11
GO:0003723	RNA binding	4	11
GO:0003727	single-stranded RNA binding	5	2
GO:0003729	mRNA binding	5	2
GO:0003730	mRNA 3'-UTR binding	6	2
GO:0005488	binding	1	11
GO:0005515	protein binding	2	2
GO:0008143	poly(A) binding	7	2
GO:0008187	poly-pyrimidine tract binding	6	2
GO:0008190	eukaryotic initiation factor 4E binding	4	2
GO:0008266	poly(U) RNA binding	7	2
GO:0031369	translation initiation factor binding	3	2
GO:0031370	eukaryotic initiation factor 4G binding	4	2
GO:0070717	poly-purine tract binding	6	2
GO:0097159	organic cyclic compound binding	2	11
GO:1901363	heterocyclic compound binding	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	165	169	PF00656	0.570
CLV_NRD_NRD_1	194	196	PF00675	0.447
CLV_NRD_NRD_1	384	386	PF00675	0.490
CLV_PCSK_KEX2_1	194	196	PF00082	0.447
CLV_PCSK_KEX2_1	384	386	PF00082	0.490
CLV_PCSK_KEX2_1	541	543	PF00082	0.341
CLV_PCSK_KEX2_1	89	91	PF00082	0.273
CLV_PCSK_PC1ET2_1	541	543	PF00082	0.341
CLV_PCSK_PC1ET2_1	89	91	PF00082	0.257
CLV_PCSK_SKI1_1	184	188	PF00082	0.487
CLV_PCSK_SKI1_1	212	216	PF00082	0.281
CLV_PCSK_SKI1_1	499	503	PF00082	0.281
CLV_PCSK_SKI1_1	505	509	PF00082	0.284
DEG_ODPH_VHL_1	513	526	PF01847	0.478
DEG_SCF_FBW7_1	465	472	PF00400	0.682
DOC_CDC14_PxL_1	511	519	PF14671	0.541
DOC_CKS1_1	456	461	PF01111	0.653
DOC_CKS1_1	466	471	PF01111	0.636
DOC_CKS1_1	477	482	PF01111	0.621
DOC_CKS1_1	484	489	PF01111	0.360
DOC_MAPK_gen_1	151	160	PF00069	0.461
DOC_MAPK_gen_1	384	394	PF00069	0.522
DOC_MAPK_MEF2A_6	388	396	PF00069	0.532
DOC_MAPK_MEF2A_6	520	528	PF00069	0.475
DOC_PP1_RVXF_1	391	397	PF00149	0.547
DOC_PP1_RVXF_1	95	101	PF00149	0.500
DOC_PP4_FxxP_1	456	459	PF00568	0.644
DOC_USP7_MATH_1	457	461	PF00917	0.654
DOC_USP7_MATH_1	82	86	PF00917	0.541
DOC_USP7_MATH_2	487	493	PF00917	0.426
DOC_USP7_UBL2_3	118	122	PF12436	0.564
DOC_USP7_UBL2_3	225	229	PF12436	0.476
DOC_WW_Pin1_4	440	445	PF00397	0.751
DOC_WW_Pin1_4	455	460	PF00397	0.671
DOC_WW_Pin1_4	465	470	PF00397	0.634
DOC_WW_Pin1_4	476	481	PF00397	0.657
DOC_WW_Pin1_4	483	488	PF00397	0.366
DOC_WW_Pin1_4	491	496	PF00397	0.475
LIG_14-3-3_CanoR_1	337	345	PF00244	0.476
LIG_14-3-3_CanoR_1	388	392	PF00244	0.548
LIG_14-3-3_CanoR_1	546	554	PF00244	0.541
LIG_Actin_WH2_2	92	110	PF00022	0.544
LIG_APCC_ABBA_1	324	329	PF00400	0.541
LIG_BIR_II_1	1	5	PF00653	0.659
LIG_BIR_III_3	1	5	PF00653	0.598
LIG_BRCT_BRCA1_1	345	349	PF00533	0.461
LIG_BRCT_BRCA1_1	84	88	PF00533	0.541
LIG_CaM_IQ_9	391	407	PF13499	0.538
LIG_FHA_1	477	483	PF00498	0.637
LIG_FHA_1	546	552	PF00498	0.492
LIG_FHA_2	317	323	PF00498	0.502
LIG_FHA_2	33	39	PF00498	0.485
LIG_FHA_2	484	490	PF00498	0.580
LIG_Integrin_isoDGR_2	368	370	PF01839	0.261
LIG_LIR_Gen_1	131	140	PF02991	0.521
LIG_LIR_Gen_1	224	233	PF02991	0.453
LIG_LIR_Gen_1	386	396	PF02991	0.523
LIG_LIR_Gen_1	504	515	PF02991	0.475
LIG_LIR_Gen_1	522	533	PF02991	0.475
LIG_LIR_Nem_3	131	136	PF02991	0.455
LIG_LIR_Nem_3	152	158	PF02991	0.461
LIG_LIR_Nem_3	224	230	PF02991	0.453
LIG_LIR_Nem_3	325	330	PF02991	0.461
LIG_LIR_Nem_3	346	352	PF02991	0.461
LIG_LIR_Nem_3	386	392	PF02991	0.525
LIG_LIR_Nem_3	43	48	PF02991	0.455
LIG_LIR_Nem_3	504	510	PF02991	0.475
LIG_LIR_Nem_3	532	537	PF02991	0.492
LIG_LIR_Nem_3	65	71	PF02991	0.457
LIG_LIR_Nem_3	85	91	PF02991	0.513
LIG_MYND_1	480	484	PF01753	0.618
LIG_MYND_1	515	519	PF01753	0.541
LIG_PCNA_TLS_4	220	227	PF02747	0.461
LIG_PCNA_yPIPBox_3	194	203	PF02747	0.442
LIG_Pex14_2	226	230	PF04695	0.461
LIG_Pex14_2	314	318	PF04695	0.461
LIG_PTB_Apo_2	304	311	PF02174	0.411
LIG_PTB_Phospho_1	304	310	PF10480	0.419
LIG_REV1ctd_RIR_1	224	233	PF16727	0.461
LIG_SH2_CRK	155	159	PF00017	0.461
LIG_SH2_CRK	389	393	PF00017	0.583
LIG_SH2_CRK	68	72	PF00017	0.492
LIG_SH2_GRB2like	70	73	PF00017	0.461
LIG_SH2_NCK_1	511	515	PF00017	0.541
LIG_SH2_SRC	511	514	PF00017	0.541
LIG_SH2_STAT3	210	213	PF00017	0.461
LIG_SH2_STAT5	188	191	PF00017	0.406
LIG_SH2_STAT5	210	213	PF00017	0.455
LIG_SH2_STAT5	28	31	PF00017	0.461
LIG_SH2_STAT5	281	284	PF00017	0.403
LIG_SH2_STAT5	310	313	PF00017	0.461
LIG_SH2_STAT5	389	392	PF00017	0.583
LIG_SH2_STAT5	70	73	PF00017	0.461
LIG_SH3_3	441	447	PF00018	0.651
LIG_SH3_3	474	480	PF00018	0.673
LIG_SH3_3	481	487	PF00018	0.600
LIG_TRAF2_1	4	7	PF00917	0.573
LIG_TRAF2_1	486	489	PF00917	0.661
LIG_TRAF2_1	494	497	PF00917	0.541
LIG_TYR_ITIM	66	71	PF00017	0.314
LIG_UBA3_1	292	298	PF00899	0.503
LIG_UBA3_1	323	328	PF00899	0.426
LIG_UBA3_1	524	531	PF00899	0.333
LIG_WRC_WIRS_1	351	356	PF05994	0.314
LIG_WRPW_2	45	48	PF00400	0.333
LIG_WW_2	480	483	PF00397	0.620
MOD_CDK_SPK_2	465	470	PF00069	0.749
MOD_CK1_1	138	144	PF00069	0.407
MOD_CK1_1	343	349	PF00069	0.459
MOD_CK2_1	32	38	PF00069	0.347
MOD_CK2_1	483	489	PF00069	0.598
MOD_CK2_1	491	497	PF00069	0.298
MOD_GlcNHglycan	109	112	PF01048	0.449
MOD_GlcNHglycan	162	167	PF01048	0.442
MOD_GlcNHglycan	234	237	PF01048	0.317
MOD_GlcNHglycan	241	245	PF01048	0.310
MOD_GlcNHglycan	342	345	PF01048	0.459
MOD_GlcNHglycan	459	462	PF01048	0.702
MOD_GlcNHglycan	77	80	PF01048	0.333
MOD_GSK3_1	131	138	PF00069	0.352
MOD_GSK3_1	465	472	PF00069	0.705
MOD_LATS_1	159	165	PF00433	0.381
MOD_N-GLC_1	204	209	PF02516	0.386
MOD_NEK2_1	107	112	PF00069	0.459
MOD_NEK2_1	32	37	PF00069	0.312
MOD_NEK2_1	413	418	PF00069	0.559
MOD_PIKK_1	413	419	PF00454	0.636
MOD_PIKK_1	59	65	PF00454	0.314
MOD_PK_1	135	141	PF00069	0.401
MOD_PKA_2	107	113	PF00069	0.451
MOD_PKA_2	387	393	PF00069	0.550
MOD_PKA_2	545	551	PF00069	0.426
MOD_Plk_1	32	38	PF00069	0.347
MOD_Plk_2-3	125	131	PF00069	0.426
MOD_Plk_2-3	178	184	PF00069	0.646
MOD_Plk_4	135	141	PF00069	0.347
MOD_Plk_4	23	29	PF00069	0.404
MOD_Plk_4	387	393	PF00069	0.550
MOD_Plk_4	47	53	PF00069	0.314
MOD_ProDKin_1	440	446	PF00069	0.753
MOD_ProDKin_1	455	461	PF00069	0.669
MOD_ProDKin_1	465	471	PF00069	0.635
MOD_ProDKin_1	476	482	PF00069	0.648
MOD_ProDKin_1	483	489	PF00069	0.364
MOD_ProDKin_1	491	497	PF00069	0.333
MOD_SUMO_for_1	160	163	PF00179	0.426
MOD_SUMO_rev_2	121	129	PF00179	0.426
MOD_SUMO_rev_2	147	152	PF00179	0.389
MOD_SUMO_rev_2	178	187	PF00179	0.526
MOD_SUMO_rev_2	329	335	PF00179	0.459
MOD_SUMO_rev_2	535	543	PF00179	0.380
TRG_DiLeu_BaEn_1	532	537	PF01217	0.357
TRG_DiLeu_BaLyEn_6	288	293	PF01217	0.472
TRG_DiLeu_BaLyEn_6	373	378	PF01217	0.333
TRG_ENDOCYTIC_2	155	158	PF00928	0.314
TRG_ENDOCYTIC_2	330	333	PF00928	0.314
TRG_ENDOCYTIC_2	389	392	PF00928	0.583
TRG_ENDOCYTIC_2	534	537	PF00928	0.426
TRG_ENDOCYTIC_2	68	71	PF00928	0.357
TRG_ER_diArg_1	193	195	PF00400	0.437
TRG_ER_diArg_1	421	424	PF00400	0.753
TRG_NES_CRM1_1	516	527	PF08389	0.426
TRG_Pf-PMV_PEXEL_1	541	545	PF00026	0.378

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P3Y8	Leptomonas seymouri	87%	99%
A0A0N0P5A9	Leptomonas seymouri	37%	99%
A0A0N1I0F0	Leptomonas seymouri	35%	100%
A0A0R4IEW8	Danio rerio	23%	100%
A0A0S4IKZ4	Bodo saltans	33%	98%
A0A0S4JPT3	Bodo saltans	37%	99%
A0A1X0P4T3	Trypanosomatidae	73%	100%
A0A1X0P4V5	Trypanosomatidae	41%	98%
A0A1X0P525	Trypanosomatidae	34%	100%
A0A3Q8IF40	Leishmania donovani	35%	100%
A0A3Q8IGR8	Leishmania donovani	99%	100%
A0A3Q8IMW2	Leishmania donovani	36%	96%
A0A3R7NAH9	Trypanosoma rangeli	75%	100%
A0A422NK82	Trypanosoma rangeli	33%	100%
A1CRM1	Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107)	35%	74%
A1D4K4	Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181)	42%	75%
A3LXL0	Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545)	36%	89%
A4HDV5	Leishmania braziliensis	35%	100%
A4HN85	Leishmania braziliensis	38%	100%
A4HNG6	Leishmania braziliensis	94%	100%
A4I150	Leishmania infantum	35%	100%
A4IBV3	Leishmania infantum	36%	96%
A4IC57	Leishmania infantum	99%	100%
A4QNI8	Xenopus tropicalis	21%	100%
A4QUF0	Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)	38%	73%
A5DM21	Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324)	38%	89%
A5DW14	Lodderomyces elongisporus (strain ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239)	38%	85%
C9ZYE6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	69%	99%
C9ZYQ8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	34%	100%
E9AFP0	Leishmania major	36%	94%
E9AX84	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	100%
E9B6U3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	36%	94%
E9B730	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	99%
F1QB54	Danio rerio	44%	88%
F4HT49	Arabidopsis thaliana	37%	100%
O04319	Arabidopsis thaliana	36%	100%
O09032	Rattus norvegicus	23%	100%
O22173	Arabidopsis thaliana	38%	85%
O64380	Arabidopsis thaliana	43%	85%
P04147	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	39%	97%
P09405	Mus musculus	23%	79%
P0CP46	Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565)	40%	83%
P0CP47	Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)	40%	83%
P11940	Homo sapiens	45%	88%
P13383	Rattus norvegicus	22%	79%
P15771	Gallus gallus	22%	81%
P19338	Homo sapiens	22%	79%
P20397	Xenopus laevis	23%	86%
P20965	Xenopus laevis	45%	88%
P21187	Drosophila melanogaster	42%	88%
P26378	Homo sapiens	23%	100%
P29341	Mus musculus	45%	88%
P31209	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	40%	86%
P39684	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	26%	92%
P42731	Arabidopsis thaliana	38%	89%
P52912	Mus musculus	22%	100%
P61286	Bos taurus	45%	88%
Q05196	Arabidopsis thaliana	42%	82%
Q0CR95	Aspergillus terreus (strain NIH 2624 / FGSC A1156)	40%	74%
Q0U1G2	Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173)	37%	75%
Q13310	Homo sapiens	37%	87%
Q1DXH0	Coccidioides immitis (strain RS)	34%	73%
Q1ZXC2	Dictyostelium discoideum	34%	69%
Q2GSX8	Chaetomium globosum (strain ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970)	39%	72%
Q4P8R9	Ustilago maydis (strain 521 / FGSC 9021)	40%	86%
Q4QA88	Leishmania major	33%	100%
Q4R4J7	Macaca fascicularis	22%	79%
Q4VXU2	Homo sapiens	37%	91%
Q4WJT7	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)	22%	68%
Q4WK03	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)	41%	74%
Q54BM2	Dictyostelium discoideum	36%	99%
Q5AI15	Candida albicans (strain SC5314 / ATCC MYA-2876)	38%	89%
Q5B630	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	36%	77%
Q5BGA9	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	23%	68%
Q5R8F7	Pongo abelii	45%	88%
Q5RF26	Pongo abelii	22%	79%
Q61701	Mus musculus	23%	100%
Q6BI95	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	37%	89%
Q6CDH3	Yarrowia lipolytica (strain CLIB 122 / E 150)	35%	89%
Q6CSV3	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	38%	95%
Q6DEY7	Xenopus tropicalis	44%	89%
Q6FKG4	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	39%	97%
Q6GR16	Xenopus laevis	44%	89%
Q6IP09	Xenopus laevis	45%	88%
Q74ZS6	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	39%	96%
Q7S6N6	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	35%	73%
Q7SZT7	Xenopus laevis	21%	100%
Q8SR30	Encephalitozoon cuniculi (strain GB-M1)	29%	100%
Q98SP8	Xenopus laevis	44%	89%
Q9EPH8	Rattus norvegicus	45%	88%
Q9FXA2	Arabidopsis thaliana	42%	83%
Q9H361	Homo sapiens	42%	89%
Q9ZQA8	Arabidopsis thaliana	34%	92%
V5BYR2	Trypanosoma cruzi	71%	99%
V5DUJ1	Trypanosoma cruzi	34%	100%