Putative tubulin tyrosine ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tubulin tyrosine ligase, putative

Species:

Leishmania major

UniProt:

E9AFW7_LEIMA

TriTrypDb:

LmjF.35.4930 , LMJLV39_350057300 , LMJSD75_350056600

Length:

725

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 21
NetGPI	no	yes: 0, no: 21

Cellular components
Term	Name	Level	Count
GO:0005929	cilium	4	4
GO:0042995	cell projection	2	4
GO:0043226	organelle	2	4
GO:0043227	membrane-bounded organelle	3	4
GO:0110165	cellular anatomical entity	1	4
GO:0120025	plasma membrane bounded cell projection	3	4

Expansion

Sequence features

E9AFW7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFW7

Function

Biological processes
Term	Name	Level	Count
GO:0000226	microtubule cytoskeleton organization	3	4
GO:0006807	nitrogen compound metabolic process	2	22
GO:0006996	organelle organization	4	4
GO:0007010	cytoskeleton organization	5	4
GO:0007017	microtubule-based process	2	4
GO:0008152	metabolic process	1	22
GO:0009987	cellular process	1	4
GO:0016043	cellular component organization	3	4
GO:0018095	protein polyglutamylation	7	4
GO:0018193	peptidyl-amino acid modification	5	4
GO:0018200	peptidyl-glutamic acid modification	6	4
GO:0019538	protein metabolic process	3	22
GO:0036211	protein modification process	4	22
GO:0043170	macromolecule metabolic process	3	22
GO:0043412	macromolecule modification	4	22
GO:0044238	primary metabolic process	2	22
GO:0071704	organic substance metabolic process	2	22
GO:0071840	cellular component organization or biogenesis	2	4
GO:1901564	organonitrogen compound metabolic process	3	22

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	19
GO:0004835	tubulin-tyrosine ligase activity	3	6
GO:0005488	binding	1	5
GO:0005515	protein binding	2	4
GO:0008092	cytoskeletal protein binding	3	4
GO:0015631	tubulin binding	4	4
GO:0016874	ligase activity	2	19
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	7
GO:0016881	acid-amino acid ligase activity	4	7
GO:0070739	protein-glutamic acid ligase activity	3	4
GO:0070740	tubulin-glutamic acid ligase activity	4	4
GO:0140096	catalytic activity, acting on a protein	2	7
GO:0000166	nucleotide binding	3	1
GO:0005524	ATP binding	5	1
GO:0017076	purine nucleotide binding	4	1
GO:0030554	adenyl nucleotide binding	5	1
GO:0032553	ribonucleotide binding	3	1
GO:0032555	purine ribonucleotide binding	4	1
GO:0032559	adenyl ribonucleotide binding	5	1
GO:0035639	purine ribonucleoside triphosphate binding	4	1
GO:0036094	small molecule binding	2	1
GO:0043167	ion binding	2	1
GO:0043168	anion binding	3	1
GO:0043169	cation binding	3	1
GO:0046872	metal ion binding	4	1
GO:0097159	organic cyclic compound binding	2	1
GO:0097367	carbohydrate derivative binding	2	1
GO:1901265	nucleoside phosphate binding	3	1
GO:1901363	heterocyclic compound binding	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	202	206	PF00656	0.712
CLV_NRD_NRD_1	175	177	PF00675	0.538
CLV_NRD_NRD_1	308	310	PF00675	0.293
CLV_NRD_NRD_1	318	320	PF00675	0.302
CLV_NRD_NRD_1	346	348	PF00675	0.359
CLV_NRD_NRD_1	408	410	PF00675	0.291
CLV_NRD_NRD_1	599	601	PF00675	0.267
CLV_NRD_NRD_1	701	703	PF00675	0.387
CLV_NRD_NRD_1	82	84	PF00675	0.675
CLV_PCSK_KEX2_1	175	177	PF00082	0.538
CLV_PCSK_KEX2_1	282	284	PF00082	0.350
CLV_PCSK_KEX2_1	318	320	PF00082	0.328
CLV_PCSK_KEX2_1	346	348	PF00082	0.341
CLV_PCSK_KEX2_1	492	494	PF00082	0.323
CLV_PCSK_KEX2_1	599	601	PF00082	0.315
CLV_PCSK_KEX2_1	701	703	PF00082	0.357
CLV_PCSK_KEX2_1	81	83	PF00082	0.647
CLV_PCSK_PC1ET2_1	175	177	PF00082	0.554
CLV_PCSK_PC1ET2_1	282	284	PF00082	0.351
CLV_PCSK_PC1ET2_1	492	494	PF00082	0.338
CLV_PCSK_PC7_1	171	177	PF00082	0.584
CLV_PCSK_SKI1_1	176	180	PF00082	0.536
CLV_PCSK_SKI1_1	282	286	PF00082	0.385
CLV_PCSK_SKI1_1	334	338	PF00082	0.339
CLV_PCSK_SKI1_1	519	523	PF00082	0.247
CLV_PCSK_SKI1_1	579	583	PF00082	0.244
CLV_PCSK_SKI1_1	642	646	PF00082	0.435
CLV_PCSK_SKI1_1	680	684	PF00082	0.322
CLV_PCSK_SKI1_1	97	101	PF00082	0.532
DEG_APCC_DBOX_1	175	183	PF00400	0.306
DEG_APCC_DBOX_1	241	249	PF00400	0.370
DEG_MDM2_SWIB_1	276	284	PF02201	0.230
DEG_SCF_FBW7_1	50	57	PF00400	0.532
DEG_SPOP_SBC_1	141	145	PF00917	0.710
DOC_CKS1_1	353	358	PF01111	0.538
DOC_CYCLIN_RxL_1	171	180	PF00134	0.412
DOC_CYCLIN_RxL_1	638	647	PF00134	0.439
DOC_MAPK_gen_1	175	183	PF00069	0.464
DOC_MAPK_gen_1	409	415	PF00069	0.502
DOC_MAPK_gen_1	559	567	PF00069	0.538
DOC_MAPK_MEF2A_6	175	183	PF00069	0.469
DOC_MAPK_MEF2A_6	559	567	PF00069	0.520
DOC_MAPK_MEF2A_6	86	95	PF00069	0.636
DOC_PP2B_LxvP_1	49	52	PF13499	0.621
DOC_PP2B_PxIxI_1	358	364	PF00149	0.457
DOC_PP4_FxxP_1	107	110	PF00568	0.667
DOC_PP4_FxxP_1	15	18	PF00568	0.523
DOC_PP4_FxxP_1	336	339	PF00568	0.444
DOC_PP4_FxxP_1	648	651	PF00568	0.397
DOC_USP7_MATH_1	118	122	PF00917	0.650
DOC_USP7_MATH_1	128	132	PF00917	0.643
DOC_USP7_MATH_1	366	370	PF00917	0.438
DOC_USP7_MATH_1	41	45	PF00917	0.669
DOC_USP7_MATH_1	52	56	PF00917	0.675
DOC_USP7_MATH_1	537	541	PF00917	0.550
DOC_USP7_MATH_1	572	576	PF00917	0.505
DOC_USP7_MATH_1	580	584	PF00917	0.478
DOC_USP7_MATH_1	633	637	PF00917	0.574
DOC_WW_Pin1_4	352	357	PF00397	0.527
DOC_WW_Pin1_4	50	55	PF00397	0.727
LIG_14-3-3_CanoR_1	171	179	PF00244	0.585
LIG_14-3-3_CanoR_1	225	231	PF00244	0.621
LIG_14-3-3_CanoR_1	268	276	PF00244	0.526
LIG_14-3-3_CanoR_1	283	287	PF00244	0.242
LIG_14-3-3_CanoR_1	493	503	PF00244	0.550
LIG_14-3-3_CanoR_1	584	593	PF00244	0.408
LIG_14-3-3_CanoR_1	642	651	PF00244	0.481
LIG_14-3-3_CanoR_1	81	86	PF00244	0.725
LIG_Actin_RPEL_3	577	596	PF02755	0.475
LIG_Actin_WH2_2	252	270	PF00022	0.432
LIG_APCC_ABBA_1	427	432	PF00400	0.491
LIG_APCC_ABBAyCdc20_2	426	432	PF00400	0.445
LIG_APCC_Cbox_2	690	696	PF00515	0.271
LIG_BIR_II_1	1	5	PF00653	0.685
LIG_BIR_III_4	327	331	PF00653	0.373
LIG_BRCT_BRCA1_1	11	15	PF00533	0.507
LIG_BRCT_BRCA1_1	191	195	PF00533	0.332
LIG_Clathr_ClatBox_1	547	551	PF01394	0.561
LIG_deltaCOP1_diTrp_1	483	491	PF00928	0.457
LIG_eIF4E_1	399	405	PF01652	0.479
LIG_FHA_1	178	184	PF00498	0.319
LIG_FHA_1	195	201	PF00498	0.631
LIG_FHA_1	302	308	PF00498	0.445
LIG_FHA_1	462	468	PF00498	0.467
LIG_FHA_1	55	61	PF00498	0.493
LIG_FHA_1	607	613	PF00498	0.655
LIG_FHA_1	74	80	PF00498	0.699
LIG_FHA_2	101	107	PF00498	0.643
LIG_FHA_2	418	424	PF00498	0.481
LIG_LIR_Apic_2	106	110	PF02991	0.682
LIG_LIR_Apic_2	12	18	PF02991	0.524
LIG_LIR_Apic_2	208	212	PF02991	0.684
LIG_LIR_Apic_2	335	339	PF02991	0.439
LIG_LIR_Apic_2	646	651	PF02991	0.383
LIG_LIR_Gen_1	229	240	PF02991	0.416
LIG_LIR_Gen_1	275	284	PF02991	0.330
LIG_LIR_Gen_1	520	529	PF02991	0.425
LIG_LIR_Nem_3	229	235	PF02991	0.387
LIG_LIR_Nem_3	275	279	PF02991	0.309
LIG_LIR_Nem_3	326	332	PF02991	0.438
LIG_LIR_Nem_3	420	425	PF02991	0.445
LIG_LIR_Nem_3	520	524	PF02991	0.425
LIG_LIR_Nem_3	690	695	PF02991	0.331
LIG_LIR_Nem_3	7	11	PF02991	0.464
LIG_LIR_Nem_3	88	93	PF02991	0.652
LIG_LYPXL_yS_3	90	93	PF13949	0.642
LIG_MLH1_MIPbox_1	191	195	PF16413	0.350
LIG_MYND_1	58	62	PF01753	0.750
LIG_Pex14_2	276	280	PF04695	0.309
LIG_Pex14_2	301	305	PF04695	0.413
LIG_Pex14_2	679	683	PF04695	0.238
LIG_PTB_Apo_2	106	113	PF02174	0.504
LIG_PTB_Apo_2	628	635	PF02174	0.365
LIG_PTB_Phospho_1	106	112	PF10480	0.500
LIG_RPA_C_Fungi	117	129	PF08784	0.716
LIG_SH2_CRK	295	299	PF00017	0.363
LIG_SH2_CRK	416	420	PF00017	0.299
LIG_SH2_PTP2	232	235	PF00017	0.332
LIG_SH2_SRC	112	115	PF00017	0.706
LIG_SH2_STAP1	669	673	PF00017	0.468
LIG_SH2_STAT5	112	115	PF00017	0.545
LIG_SH2_STAT5	199	202	PF00017	0.641
LIG_SH2_STAT5	232	235	PF00017	0.496
LIG_SH2_STAT5	269	272	PF00017	0.363
LIG_SH2_STAT5	287	290	PF00017	0.362
LIG_SH2_STAT5	323	326	PF00017	0.214
LIG_SH2_STAT5	362	365	PF00017	0.304
LIG_SH2_STAT5	399	402	PF00017	0.298
LIG_SH2_STAT5	416	419	PF00017	0.339
LIG_SH2_STAT5	428	431	PF00017	0.343
LIG_SH2_STAT5	613	616	PF00017	0.583
LIG_SH2_STAT5	657	660	PF00017	0.467
LIG_SH2_STAT5	692	695	PF00017	0.297
LIG_SH2_STAT5	705	708	PF00017	0.308
LIG_SH2_STAT5	98	101	PF00017	0.591
LIG_SH3_1	379	385	PF00018	0.300
LIG_SH3_2	353	358	PF14604	0.413
LIG_SH3_3	178	184	PF00018	0.387
LIG_SH3_3	350	356	PF00018	0.423
LIG_SH3_3	379	385	PF00018	0.281
LIG_SH3_3	637	643	PF00018	0.433
LIG_SH3_3	96	102	PF00018	0.590
LIG_TRAF2_2	445	450	PF00917	0.413
LIG_TRFH_1	362	366	PF08558	0.381
LIG_TYR_ITIM	230	235	PF00017	0.337
LIG_UBA3_1	360	365	PF00899	0.300
LIG_WRC_WIRS_1	419	424	PF05994	0.281
LIG_WRC_WIRS_1	518	523	PF05994	0.254
LIG_WRC_WIRS_1	645	650	PF05994	0.320
MOD_CDK_SPxK_1	352	358	PF00069	0.413
MOD_CK1_1	121	127	PF00069	0.692
MOD_CK1_1	139	145	PF00069	0.463
MOD_CK1_1	149	155	PF00069	0.522
MOD_CK1_1	189	195	PF00069	0.350
MOD_CK1_1	231	237	PF00069	0.460
MOD_CK1_1	4	10	PF00069	0.623
MOD_CK1_1	468	474	PF00069	0.388
MOD_CK1_1	56	62	PF00069	0.655
MOD_CK1_1	570	576	PF00069	0.253
MOD_CK1_1	656	662	PF00069	0.388
MOD_CK2_1	231	237	PF00069	0.612
MOD_CK2_1	656	662	PF00069	0.398
MOD_Cter_Amidation	307	310	PF01082	0.282
MOD_Cter_Amidation	407	410	PF01082	0.347
MOD_GlcNHglycan	1	4	PF01048	0.688
MOD_GlcNHglycan	11	14	PF01048	0.693
MOD_GlcNHglycan	188	191	PF01048	0.469
MOD_GlcNHglycan	255	258	PF01048	0.400
MOD_GlcNHglycan	442	445	PF01048	0.441
MOD_GlcNHglycan	538	542	PF01048	0.393
MOD_GlcNHglycan	635	638	PF01048	0.600
MOD_GlcNHglycan	708	711	PF01048	0.336
MOD_GSK3_1	114	121	PF00069	0.603
MOD_GSK3_1	136	143	PF00069	0.585
MOD_GSK3_1	146	153	PF00069	0.612
MOD_GSK3_1	154	161	PF00069	0.607
MOD_GSK3_1	185	192	PF00069	0.449
MOD_GSK3_1	457	464	PF00069	0.303
MOD_GSK3_1	50	57	PF00069	0.719
MOD_GSK3_1	580	587	PF00069	0.371
MOD_GSK3_1	649	656	PF00069	0.386
MOD_GSK3_1	73	80	PF00069	0.624
MOD_GSK3_1	81	88	PF00069	0.588
MOD_N-GLC_1	226	231	PF02516	0.566
MOD_N-GLC_1	457	462	PF02516	0.302
MOD_N-GLC_1	606	611	PF02516	0.543
MOD_NEK2_1	1	6	PF00069	0.686
MOD_NEK2_1	170	175	PF00069	0.672
MOD_NEK2_1	193	198	PF00069	0.398
MOD_NEK2_1	228	233	PF00069	0.436
MOD_NEK2_1	644	649	PF00069	0.387
MOD_NEK2_1	706	711	PF00069	0.327
MOD_PIKK_1	599	605	PF00454	0.578
MOD_PKA_1	282	288	PF00069	0.446
MOD_PKA_1	440	446	PF00069	0.230
MOD_PKA_1	599	605	PF00069	0.561
MOD_PKA_1	81	87	PF00069	0.708
MOD_PKA_2	121	127	PF00069	0.647
MOD_PKA_2	139	145	PF00069	0.556
MOD_PKA_2	158	164	PF00069	0.742
MOD_PKA_2	170	176	PF00069	0.704
MOD_PKA_2	267	273	PF00069	0.494
MOD_PKA_2	282	288	PF00069	0.449
MOD_PKA_2	599	605	PF00069	0.562
MOD_PKA_2	700	706	PF00069	0.391
MOD_PKA_2	81	87	PF00069	0.663
MOD_Plk_1	226	232	PF00069	0.562
MOD_Plk_1	457	463	PF00069	0.289
MOD_Plk_1	623	629	PF00069	0.496
MOD_Plk_1	653	659	PF00069	0.521
MOD_Plk_2-3	475	481	PF00069	0.300
MOD_Plk_4	177	183	PF00069	0.381
MOD_Plk_4	189	195	PF00069	0.351
MOD_Plk_4	228	234	PF00069	0.537
MOD_Plk_4	282	288	PF00069	0.535
MOD_Plk_4	332	338	PF00069	0.320
MOD_Plk_4	517	523	PF00069	0.383
MOD_Plk_4	56	62	PF00069	0.602
MOD_Plk_4	588	594	PF00069	0.292
MOD_Plk_4	653	659	PF00069	0.380
MOD_Plk_4	660	666	PF00069	0.386
MOD_ProDKin_1	352	358	PF00069	0.398
MOD_ProDKin_1	50	56	PF00069	0.725
MOD_SUMO_rev_2	551	560	PF00179	0.182
TRG_DiLeu_BaEn_4	165	171	PF01217	0.679
TRG_DiLeu_BaLyEn_6	356	361	PF01217	0.300
TRG_DiLeu_BaLyEn_6	400	405	PF01217	0.349
TRG_DiLeu_BaLyEn_6	558	563	PF01217	0.300
TRG_DiLeu_BaLyEn_6	640	645	PF01217	0.429
TRG_ENDOCYTIC_2	232	235	PF00928	0.452
TRG_ENDOCYTIC_2	295	298	PF00928	0.347
TRG_ENDOCYTIC_2	416	419	PF00928	0.297
TRG_ENDOCYTIC_2	90	93	PF00928	0.642
TRG_ER_diArg_1	317	319	PF00400	0.442
TRG_ER_diArg_1	345	347	PF00400	0.442
TRG_ER_diArg_1	598	600	PF00400	0.413
TRG_ER_diArg_1	700	702	PF00400	0.449
TRG_ER_diArg_1	81	83	PF00400	0.706

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I1Q6	Leptomonas seymouri	26%	100%
A0A0N1IAP5	Leptomonas seymouri	77%	98%
A0A0S4J7C9	Bodo saltans	51%	100%
A0A1X0NXX8	Trypanosomatidae	30%	100%
A0A1X0P6A0	Trypanosomatidae	55%	100%
A0A3Q8III2	Leishmania donovani	95%	100%
A0A3S7X261	Leishmania donovani	26%	100%
A0A422NPL9	Trypanosoma rangeli	32%	100%
A0A422P136	Trypanosoma rangeli	54%	100%
A4HNF6	Leishmania braziliensis	85%	100%
A4I479	Leishmania infantum	26%	100%
A4IC28	Leishmania infantum	95%	100%
C9ZI96	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	28%	100%
C9ZYH1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	52%	100%
E9ADM8	Leishmania major	26%	100%
E9AID0	Leishmania braziliensis	28%	97%
E9AM52	Leishmania mexicana (strain MHOM/GT/2001/U1103)	27%	100%
E9B720	Leishmania mexicana (strain MHOM/GT/2001/U1103)	94%	100%
Q4QCW7	Leishmania major	27%	100%
V5BVF5	Trypanosoma cruzi	30%	100%