Putative DNA replication factor

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DNA replication licensing factor MCM9, putative

Species:

Leishmania major

UniProt:

E9AFW6_LEIMA

TriTrypDb:

LmjF.35.4910 , LMJLV39_350057200 , LMJSD75_350056500

Length:

908

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0005654	nucleoplasm	2	2
GO:0032991	protein-containing complex	1	2
GO:0042555	MCM complex	2	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9AFW6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFW6

Function

Biological processes
Term	Name	Level	Count
GO:0000724	double-strand break repair via homologous recombination	7	2
GO:0000725	recombinational repair	6	2
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006259	DNA metabolic process	4	2
GO:0006281	DNA repair	5	2
GO:0006302	double-strand break repair	6	2
GO:0006310	DNA recombination	5	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0006950	response to stress	2	2
GO:0006974	DNA damage response	4	2
GO:0006996	organelle organization	4	11
GO:0008152	metabolic process	1	2
GO:0009987	cellular process	1	11
GO:0016043	cellular component organization	3	11
GO:0032392	DNA geometric change	7	11
GO:0032508	DNA duplex unwinding	8	11
GO:0033554	cellular response to stress	3	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0043170	macromolecule metabolic process	3	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0046483	heterocycle metabolic process	3	2
GO:0050896	response to stimulus	1	2
GO:0051276	chromosome organization	5	11
GO:0051716	cellular response to stimulus	2	2
GO:0071103	DNA conformation change	6	11
GO:0071704	organic substance metabolic process	2	2
GO:0071840	cellular component organization or biogenesis	2	11
GO:0090304	nucleic acid metabolic process	4	2
GO:1901360	organic cyclic compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	11
GO:0003676	nucleic acid binding	3	11
GO:0003677	DNA binding	4	11
GO:0003697	single-stranded DNA binding	5	2
GO:0005488	binding	1	11
GO:0005524	ATP binding	5	11
GO:0017076	purine nucleotide binding	4	11
GO:0030554	adenyl nucleotide binding	5	11
GO:0032553	ribonucleotide binding	3	11
GO:0032555	purine ribonucleotide binding	4	11
GO:0032559	adenyl ribonucleotide binding	5	11
GO:0035639	purine ribonucleoside triphosphate binding	4	11
GO:0036094	small molecule binding	2	11
GO:0043167	ion binding	2	11
GO:0043168	anion binding	3	11
GO:0097159	organic cyclic compound binding	2	11
GO:0097367	carbohydrate derivative binding	2	11
GO:1901265	nucleoside phosphate binding	3	11
GO:1901363	heterocyclic compound binding	2	11
GO:0003824	catalytic activity	1	1
GO:0016740	transferase activity	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	724	728	PF00656	0.592
CLV_NRD_NRD_1	286	288	PF00675	0.653
CLV_NRD_NRD_1	309	311	PF00675	0.445
CLV_NRD_NRD_1	472	474	PF00675	0.304
CLV_NRD_NRD_1	733	735	PF00675	0.724
CLV_NRD_NRD_1	798	800	PF00675	0.473
CLV_NRD_NRD_1	892	894	PF00675	0.648
CLV_PCSK_KEX2_1	143	145	PF00082	0.265
CLV_PCSK_KEX2_1	285	287	PF00082	0.656
CLV_PCSK_KEX2_1	309	311	PF00082	0.445
CLV_PCSK_KEX2_1	472	474	PF00082	0.282
CLV_PCSK_KEX2_1	798	800	PF00082	0.473
CLV_PCSK_KEX2_1	894	896	PF00082	0.579
CLV_PCSK_PC1ET2_1	143	145	PF00082	0.265
CLV_PCSK_PC1ET2_1	309	311	PF00082	0.509
CLV_PCSK_PC1ET2_1	894	896	PF00082	0.631
CLV_PCSK_PC7_1	139	145	PF00082	0.265
CLV_PCSK_SKI1_1	215	219	PF00082	0.251
CLV_PCSK_SKI1_1	246	250	PF00082	0.540
CLV_PCSK_SKI1_1	312	316	PF00082	0.329
CLV_PCSK_SKI1_1	51	55	PF00082	0.495
DEG_APCC_DBOX_1	214	222	PF00400	0.451
DEG_Nend_UBRbox_2	1	3	PF02207	0.642
DEG_SCF_FBW7_2	495	502	PF00400	0.481
DEG_SCF_TRCP1_1	849	854	PF00400	0.530
DEG_SPOP_SBC_1	274	278	PF00917	0.667
DEG_SPOP_SBC_1	37	41	PF00917	0.732
DEG_SPOP_SBC_1	380	384	PF00917	0.451
DEG_SPOP_SBC_1	712	716	PF00917	0.659
DEG_SPOP_SBC_1	76	80	PF00917	0.473
DOC_CKS1_1	496	501	PF01111	0.481
DOC_CYCLIN_yCln2_LP_2	624	630	PF00134	0.532
DOC_MAPK_gen_1	307	316	PF00069	0.510
DOC_MAPK_gen_1	472	481	PF00069	0.531
DOC_MAPK_MEF2A_6	109	117	PF00069	0.548
DOC_MAPK_MEF2A_6	685	692	PF00069	0.482
DOC_MAPK_MEF2A_6	868	877	PF00069	0.656
DOC_PP1_RVXF_1	532	539	PF00149	0.358
DOC_PP1_RVXF_1	900	907	PF00149	0.595
DOC_PP2B_LxvP_1	327	330	PF13499	0.465
DOC_PP2B_LxvP_1	823	826	PF13499	0.535
DOC_PP2B_LxvP_1	845	848	PF13499	0.704
DOC_PP2B_LxvP_1	875	878	PF13499	0.710
DOC_PP4_FxxP_1	164	167	PF00568	0.526
DOC_SPAK_OSR1_1	331	335	PF12202	0.499
DOC_USP7_MATH_1	147	151	PF00917	0.531
DOC_USP7_MATH_1	274	278	PF00917	0.621
DOC_USP7_MATH_1	37	41	PF00917	0.666
DOC_USP7_MATH_1	704	708	PF00917	0.680
DOC_USP7_MATH_1	712	716	PF00917	0.698
DOC_USP7_MATH_1	748	752	PF00917	0.579
DOC_USP7_MATH_1	773	777	PF00917	0.653
DOC_USP7_MATH_1	855	859	PF00917	0.776
DOC_USP7_MATH_1	871	875	PF00917	0.768
DOC_USP7_UBL2_3	307	311	PF12436	0.538
DOC_WW_Pin1_4	33	38	PF00397	0.737
DOC_WW_Pin1_4	427	432	PF00397	0.531
DOC_WW_Pin1_4	495	500	PF00397	0.481
DOC_WW_Pin1_4	569	574	PF00397	0.481
DOC_WW_Pin1_4	636	641	PF00397	0.504
DOC_WW_Pin1_4	781	786	PF00397	0.647
DOC_WW_Pin1_4	811	816	PF00397	0.776
DOC_WW_Pin1_4	869	874	PF00397	0.751
LIG_14-3-3_CanoR_1	109	117	PF00244	0.424
LIG_14-3-3_CanoR_1	139	147	PF00244	0.531
LIG_14-3-3_CanoR_1	156	164	PF00244	0.531
LIG_14-3-3_CanoR_1	272	282	PF00244	0.695
LIG_14-3-3_CanoR_1	287	296	PF00244	0.522
LIG_14-3-3_CanoR_1	312	317	PF00244	0.504
LIG_14-3-3_CanoR_1	381	388	PF00244	0.451
LIG_14-3-3_CanoR_1	434	438	PF00244	0.465
LIG_14-3-3_CanoR_1	473	481	PF00244	0.496
LIG_14-3-3_CanoR_1	591	599	PF00244	0.466
LIG_14-3-3_CanoR_1	619	625	PF00244	0.520
LIG_14-3-3_CanoR_1	798	804	PF00244	0.702
LIG_14-3-3_CanoR_1	893	901	PF00244	0.645
LIG_BIR_III_2	577	581	PF00653	0.503
LIG_BRCT_BRCA1_1	160	164	PF00533	0.404
LIG_BRCT_BRCA1_1	324	328	PF00533	0.481
LIG_BRCT_BRCA1_1	902	906	PF00533	0.598
LIG_Clathr_ClatBox_1	218	222	PF01394	0.451
LIG_CtBP_PxDLS_1	114	118	PF00389	0.386
LIG_EH_1	165	169	PF12763	0.497
LIG_FHA_1	140	146	PF00498	0.498
LIG_FHA_1	158	164	PF00498	0.369
LIG_FHA_1	2	8	PF00498	0.492
LIG_FHA_1	367	373	PF00498	0.513
LIG_FHA_1	443	449	PF00498	0.462
LIG_FHA_1	714	720	PF00498	0.662
LIG_FHA_1	76	82	PF00498	0.408
LIG_FHA_2	218	224	PF00498	0.465
LIG_FHA_2	4	10	PF00498	0.496
LIG_FHA_2	526	532	PF00498	0.515
LIG_FHA_2	604	610	PF00498	0.451
LIG_FHA_2	682	688	PF00498	0.696
LIG_FHA_2	718	724	PF00498	0.678
LIG_FHA_2	78	84	PF00498	0.454
LIG_FHA_2	830	836	PF00498	0.564
LIG_Integrin_RGD_1	685	687	PF01839	0.641
LIG_LIR_Apic_2	161	167	PF02991	0.524
LIG_LIR_Gen_1	487	495	PF02991	0.451
LIG_LIR_Gen_1	827	835	PF02991	0.580
LIG_LIR_Gen_1	882	889	PF02991	0.674
LIG_LIR_Nem_3	299	305	PF02991	0.448
LIG_LIR_Nem_3	487	492	PF02991	0.451
LIG_LIR_Nem_3	560	565	PF02991	0.435
LIG_LIR_Nem_3	827	831	PF02991	0.652
LIG_LIR_Nem_3	882	888	PF02991	0.678
LIG_PDZ_Class_2	903	908	PF00595	0.502
LIG_Pex14_1	245	249	PF04695	0.477
LIG_Pex14_2	164	168	PF04695	0.554
LIG_Pex14_2	301	305	PF04695	0.453
LIG_Pex14_2	328	332	PF04695	0.499
LIG_Pex14_2	489	493	PF04695	0.442
LIG_SH2_CRK	671	675	PF00017	0.618
LIG_SH2_CRK	828	832	PF00017	0.577
LIG_SH2_CRK	885	889	PF00017	0.675
LIG_SH2_NCK_1	543	547	PF00017	0.526
LIG_SH2_STAP1	148	152	PF00017	0.531
LIG_SH2_STAT3	563	566	PF00017	0.465
LIG_SH2_STAT5	13	16	PF00017	0.493
LIG_SH2_STAT5	360	363	PF00017	0.554
LIG_SH2_STAT5	535	538	PF00017	0.344
LIG_SH2_STAT5	828	831	PF00017	0.655
LIG_SH3_3	118	124	PF00018	0.526
LIG_SH3_3	18	24	PF00018	0.592
LIG_SH3_3	358	364	PF00018	0.465
LIG_SH3_3	404	410	PF00018	0.451
LIG_SH3_3	478	484	PF00018	0.451
LIG_SH3_3	810	816	PF00018	0.760
LIG_SH3_3	863	869	PF00018	0.766
LIG_SUMO_SIM_par_1	216	224	PF11976	0.453
LIG_SUMO_SIM_par_1	745	751	PF11976	0.677
LIG_TRFH_1	885	889	PF08558	0.753
LIG_TYR_ITIM	11	16	PF00017	0.522
LIG_TYR_ITIM	883	888	PF00017	0.677
LIG_UBA3_1	137	143	PF00899	0.416
LIG_UBA3_1	492	500	PF00899	0.363
LIG_WRC_WIRS_1	302	307	PF05994	0.471
LIG_WW_2	21	24	PF00397	0.440
MOD_CDK_SPK_2	495	500	PF00069	0.371
MOD_CDK_SPK_2	781	786	PF00069	0.636
MOD_CDK_SPxxK_3	427	434	PF00069	0.416
MOD_CK1_1	104	110	PF00069	0.472
MOD_CK1_1	207	213	PF00069	0.347
MOD_CK1_1	252	258	PF00069	0.573
MOD_CK1_1	36	42	PF00069	0.685
MOD_CK1_1	382	388	PF00069	0.304
MOD_CK1_1	707	713	PF00069	0.696
MOD_CK1_1	780	786	PF00069	0.725
MOD_CK1_1	811	817	PF00069	0.718
MOD_CK1_1	854	860	PF00069	0.747
MOD_CK2_1	3	9	PF00069	0.501
MOD_CK2_1	433	439	PF00069	0.304
MOD_CK2_1	525	531	PF00069	0.511
MOD_CK2_1	635	641	PF00069	0.609
MOD_CK2_1	772	778	PF00069	0.752
MOD_CK2_1	829	835	PF00069	0.731
MOD_CK2_1	873	879	PF00069	0.680
MOD_GlcNHglycan	209	212	PF01048	0.323
MOD_GlcNHglycan	278	281	PF01048	0.631
MOD_GlcNHglycan	31	34	PF01048	0.660
MOD_GlcNHglycan	324	327	PF01048	0.335
MOD_GlcNHglycan	336	339	PF01048	0.347
MOD_GlcNHglycan	391	394	PF01048	0.304
MOD_GlcNHglycan	40	43	PF01048	0.654
MOD_GlcNHglycan	464	467	PF01048	0.304
MOD_GlcNHglycan	661	664	PF01048	0.463
MOD_GlcNHglycan	674	677	PF01048	0.581
MOD_GlcNHglycan	737	740	PF01048	0.623
MOD_GlcNHglycan	750	753	PF01048	0.578
MOD_GlcNHglycan	762	765	PF01048	0.671
MOD_GlcNHglycan	778	782	PF01048	0.512
MOD_GlcNHglycan	799	802	PF01048	0.674
MOD_GlcNHglycan	805	808	PF01048	0.726
MOD_GlcNHglycan	849	852	PF01048	0.697
MOD_GlcNHglycan	853	856	PF01048	0.739
MOD_GlcNHglycan	857	860	PF01048	0.715
MOD_GlcNHglycan	895	898	PF01048	0.706
MOD_GSK3_1	100	107	PF00069	0.450
MOD_GSK3_1	115	122	PF00069	0.557
MOD_GSK3_1	158	165	PF00069	0.337
MOD_GSK3_1	169	176	PF00069	0.369
MOD_GSK3_1	213	220	PF00069	0.313
MOD_GSK3_1	223	230	PF00069	0.288
MOD_GSK3_1	24	31	PF00069	0.618
MOD_GSK3_1	248	255	PF00069	0.542
MOD_GSK3_1	312	319	PF00069	0.328
MOD_GSK3_1	33	40	PF00069	0.626
MOD_GSK3_1	341	348	PF00069	0.327
MOD_GSK3_1	362	369	PF00069	0.304
MOD_GSK3_1	379	386	PF00069	0.304
MOD_GSK3_1	389	396	PF00069	0.304
MOD_GSK3_1	413	420	PF00069	0.364
MOD_GSK3_1	452	459	PF00069	0.304
MOD_GSK3_1	513	520	PF00069	0.585
MOD_GSK3_1	538	545	PF00069	0.479
MOD_GSK3_1	707	714	PF00069	0.686
MOD_GSK3_1	773	780	PF00069	0.603
MOD_GSK3_1	799	806	PF00069	0.674
MOD_GSK3_1	826	833	PF00069	0.616
MOD_GSK3_1	847	854	PF00069	0.728
MOD_GSK3_1	855	862	PF00069	0.747
MOD_GSK3_1	869	876	PF00069	0.788
MOD_GSK3_1	879	886	PF00069	0.664
MOD_GSK3_1	889	896	PF00069	0.625
MOD_N-GLC_1	158	163	PF02516	0.337
MOD_N-GLC_1	207	212	PF02516	0.416
MOD_N-GLC_1	679	684	PF02516	0.534
MOD_N-GLC_1	710	715	PF02516	0.731
MOD_N-GLC_1	879	884	PF02516	0.683
MOD_N-GLC_1	889	894	PF02516	0.733
MOD_NEK2_1	102	107	PF00069	0.542
MOD_NEK2_1	133	138	PF00069	0.368
MOD_NEK2_1	227	232	PF00069	0.375
MOD_NEK2_1	301	306	PF00069	0.445
MOD_NEK2_1	316	321	PF00069	0.286
MOD_NEK2_1	388	393	PF00069	0.377
MOD_NEK2_1	585	590	PF00069	0.304
MOD_NEK2_1	617	622	PF00069	0.379
MOD_NEK2_1	635	640	PF00069	0.549
MOD_NEK2_1	75	80	PF00069	0.411
MOD_NEK2_1	791	796	PF00069	0.640
MOD_NEK2_1	808	813	PF00069	0.661
MOD_NEK2_2	346	351	PF00069	0.448
MOD_NEK2_2	49	54	PF00069	0.493
MOD_PIKK_1	173	179	PF00454	0.410
MOD_PIKK_1	366	372	PF00454	0.304
MOD_PIKK_1	538	544	PF00454	0.434
MOD_PIKK_1	590	596	PF00454	0.325
MOD_PIKK_1	704	710	PF00454	0.730
MOD_PIKK_1	791	797	PF00454	0.643
MOD_PIKK_1	808	814	PF00454	0.739
MOD_PIKK_1	826	832	PF00454	0.736
MOD_PIKK_1	859	865	PF00454	0.747
MOD_PK_1	799	805	PF00069	0.621
MOD_PKA_1	893	899	PF00069	0.641
MOD_PKA_2	108	114	PF00069	0.391
MOD_PKA_2	138	144	PF00069	0.375
MOD_PKA_2	157	163	PF00069	0.163
MOD_PKA_2	169	175	PF00069	0.321
MOD_PKA_2	252	258	PF00069	0.499
MOD_PKA_2	352	358	PF00069	0.304
MOD_PKA_2	380	386	PF00069	0.304
MOD_PKA_2	433	439	PF00069	0.304
MOD_PKA_2	590	596	PF00069	0.325
MOD_PKA_2	760	766	PF00069	0.702
MOD_PKA_2	797	803	PF00069	0.693
MOD_PKB_1	156	164	PF00069	0.337
MOD_PKB_1	310	318	PF00069	0.378
MOD_Plk_1	223	229	PF00069	0.377
MOD_Plk_1	525	531	PF00069	0.507
MOD_Plk_1	617	623	PF00069	0.430
MOD_Plk_1	826	832	PF00069	0.574
MOD_Plk_2-3	433	439	PF00069	0.304
MOD_Plk_2-3	525	531	PF00069	0.530
MOD_Plk_4	133	139	PF00069	0.304
MOD_Plk_4	517	523	PF00069	0.532
MOD_Plk_4	585	591	PF00069	0.304
MOD_Plk_4	726	732	PF00069	0.650
MOD_Plk_4	742	748	PF00069	0.581
MOD_Plk_4	77	83	PF00069	0.426
MOD_Plk_4	883	889	PF00069	0.766
MOD_ProDKin_1	33	39	PF00069	0.737
MOD_ProDKin_1	427	433	PF00069	0.416
MOD_ProDKin_1	495	501	PF00069	0.347
MOD_ProDKin_1	569	575	PF00069	0.347
MOD_ProDKin_1	636	642	PF00069	0.495
MOD_ProDKin_1	781	787	PF00069	0.643
MOD_ProDKin_1	811	817	PF00069	0.776
MOD_ProDKin_1	869	875	PF00069	0.752
MOD_SUMO_for_1	499	502	PF00179	0.371
MOD_SUMO_rev_2	169	176	PF00179	0.416
TRG_DiLeu_BaEn_1	409	414	PF01217	0.304
TRG_DiLeu_BaEn_1	842	847	PF01217	0.728
TRG_DiLeu_BaLyEn_6	481	486	PF01217	0.304
TRG_ENDOCYTIC_2	13	16	PF00928	0.507
TRG_ENDOCYTIC_2	562	565	PF00928	0.323
TRG_ENDOCYTIC_2	828	831	PF00928	0.655
TRG_ENDOCYTIC_2	885	888	PF00928	0.682
TRG_ER_diArg_1	155	158	PF00400	0.377
TRG_ER_diArg_1	284	287	PF00400	0.676
TRG_ER_diArg_1	797	799	PF00400	0.504
TRG_NES_CRM1_1	186	200	PF08389	0.324
TRG_NLS_MonoCore_2	308	313	PF00514	0.541
TRG_NLS_MonoExtC_3	892	898	PF00514	0.684
TRG_NLS_MonoExtN_4	307	314	PF00514	0.518

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P860	Leptomonas seymouri	28%	91%
A0A0N0P9H0	Leptomonas seymouri	73%	98%
A0A0S4IYQ3	Bodo saltans	26%	100%
A0A0S4J4D4	Bodo saltans	45%	97%
A0A1X0NZT6	Trypanosomatidae	29%	96%
A0A1X0P4W1	Trypanosomatidae	59%	100%
A0A3Q8IQ79	Leishmania donovani	94%	100%
A0A3R7MUG3	Trypanosoma rangeli	59%	100%
A0A3S7WY81	Leishmania donovani	27%	100%
A4HDE7	Leishmania braziliensis	26%	100%
A4HGC9	Leishmania braziliensis	29%	94%
A4HNF5	Leishmania braziliensis	85%	99%
A4I0T0	Leishmania infantum	27%	100%
A4IC27	Leishmania infantum	94%	100%
B8B406	Oryza sativa subsp. indica	32%	100%
C9ZYH2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	57%	100%
D0A7X6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	27%	96%
E9AWT2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	27%	100%
E9B719	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%
Q4QAP2	Leishmania major	26%	100%
Q69QA6	Oryza sativa subsp. japonica	31%	100%