TPR_REGION domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

TPR repeat, putative

Species:

Leishmania major

UniProt:

E9AFW3_LEIMA

TriTrypDb:

LmjF.35.4880 , LMJLV39_350056900 , LMJSD75_350056200

Length:

382

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005783	endoplasmic reticulum	5	2
GO:0016020	membrane	2	10
GO:0032991	protein-containing complex	1	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0072379	ER membrane insertion complex	2	2
GO:0072380	TRC complex	3	2
GO:0110165	cellular anatomical entity	1	10

Expansion

Sequence features

E9AFW3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFW3

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	2
GO:0006511	ubiquitin-dependent protein catabolic process	7	2
GO:0006605	protein targeting	5	2
GO:0006612	protein targeting to membrane	5	2
GO:0006620	post-translational protein targeting to endoplasmic reticulum membrane	6	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0006810	transport	3	2
GO:0006886	intracellular protein transport	4	2
GO:0006950	response to stress	2	2
GO:0008104	protein localization	4	2
GO:0008152	metabolic process	1	2
GO:0009056	catabolic process	2	2
GO:0009057	macromolecule catabolic process	4	2
GO:0009987	cellular process	1	4
GO:0010033	response to organic substance	3	2
GO:0010243	response to organonitrogen compound	4	2
GO:0010498	proteasomal protein catabolic process	5	2
GO:0015031	protein transport	4	2
GO:0019538	protein metabolic process	3	2
GO:0019941	modification-dependent protein catabolic process	6	2
GO:0030163	protein catabolic process	4	2
GO:0030433	ubiquitin-dependent ERAD pathway	6	2
GO:0033036	macromolecule localization	2	2
GO:0033365	protein localization to organelle	5	2
GO:0033554	cellular response to stress	3	2
GO:0034976	response to endoplasmic reticulum stress	4	2
GO:0036503	ERAD pathway	5	2
GO:0042221	response to chemical	2	2
GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process	6	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043632	modification-dependent macromolecule catabolic process	5	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044248	cellular catabolic process	3	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0044265	obsolete cellular macromolecule catabolic process	4	2
GO:0045047	protein targeting to ER	6	2
GO:0045184	establishment of protein localization	3	2
GO:0046907	intracellular transport	3	2
GO:0050896	response to stimulus	1	2
GO:0051179	localization	1	2
GO:0051234	establishment of localization	2	2
GO:0051603	proteolysis involved in protein catabolic process	5	2
GO:0051641	cellular localization	2	2
GO:0051649	establishment of localization in cell	3	2
GO:0051668	localization within membrane	3	2
GO:0051716	cellular response to stimulus	2	2
GO:0070727	cellular macromolecule localization	3	2
GO:0070972	protein localization to endoplasmic reticulum	6	2
GO:0071702	organic substance transport	4	2
GO:0071704	organic substance metabolic process	2	2
GO:0071705	nitrogen compound transport	4	2
GO:0072594	establishment of protein localization to organelle	4	2
GO:0072599	establishment of protein localization to endoplasmic reticulum	5	2
GO:0072657	protein localization to membrane	4	2
GO:0090150	establishment of protein localization to membrane	4	2
GO:1901564	organonitrogen compound metabolic process	3	2
GO:1901565	organonitrogen compound catabolic process	4	2
GO:1901575	organic substance catabolic process	3	2
GO:1901698	response to nitrogen compound	3	2
GO:0006457	protein folding	2	2

Molecular functions
Term	Name	Level	Count
GO:0060090	molecular adaptor activity	1	2
GO:0005488	binding	1	2
GO:0005515	protein binding	2	2
GO:0030544	Hsp70 protein binding	4	2
GO:0031072	heat shock protein binding	3	2
GO:0051879	Hsp90 protein binding	4	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	108	112	PF00656	0.615
CLV_C14_Caspase3-7	293	297	PF00656	0.296
CLV_NRD_NRD_1	132	134	PF00675	0.529
CLV_PCSK_KEX2_1	132	134	PF00082	0.529
CLV_PCSK_KEX2_1	269	271	PF00082	0.619
CLV_PCSK_PC1ET2_1	269	271	PF00082	0.619
CLV_PCSK_SKI1_1	215	219	PF00082	0.359
CLV_PCSK_SKI1_1	299	303	PF00082	0.583
CLV_PCSK_SKI1_1	340	344	PF00082	0.350
CLV_PCSK_SKI1_1	90	94	PF00082	0.425
DOC_CYCLIN_RxL_1	340	351	PF00134	0.486
DOC_MAPK_gen_1	213	220	PF00069	0.654
DOC_MAPK_gen_1	247	256	PF00069	0.246
DOC_MAPK_MEF2A_6	249	258	PF00069	0.447
DOC_PP1_RVXF_1	247	254	PF00149	0.480
DOC_PP1_RVXF_1	338	344	PF00149	0.574
DOC_PP4_FxxP_1	283	286	PF00568	0.301
DOC_USP7_MATH_1	122	126	PF00917	0.730
DOC_USP7_MATH_1	63	67	PF00917	0.566
DOC_USP7_UBL2_3	106	110	PF12436	0.619
DOC_USP7_UBL2_3	340	344	PF12436	0.630
DOC_USP7_UBL2_3	54	58	PF12436	0.618
LIG_14-3-3_CanoR_1	299	308	PF00244	0.437
LIG_14-3-3_CanoR_1	352	360	PF00244	0.636
LIG_BRCT_BRCA1_1	233	237	PF00533	0.447
LIG_BRCT_BRCA1_1	36	40	PF00533	0.586
LIG_EH1_1	260	268	PF00400	0.416
LIG_eIF4E_1	261	267	PF01652	0.389
LIG_FHA_1	162	168	PF00498	0.575
LIG_FHA_1	300	306	PF00498	0.382
LIG_FHA_1	36	42	PF00498	0.565
LIG_LIR_Gen_1	158	167	PF02991	0.637
LIG_LIR_Gen_1	194	205	PF02991	0.460
LIG_LIR_Gen_1	230	239	PF02991	0.540
LIG_LIR_Gen_1	319	329	PF02991	0.476
LIG_LIR_Gen_1	336	346	PF02991	0.545
LIG_LIR_Nem_3	158	162	PF02991	0.645
LIG_LIR_Nem_3	194	200	PF02991	0.451
LIG_LIR_Nem_3	230	235	PF02991	0.535
LIG_LIR_Nem_3	319	324	PF02991	0.362
LIG_LIR_Nem_3	336	341	PF02991	0.537
LIG_LIR_Nem_3	37	43	PF02991	0.562
LIG_Pex14_1	228	232	PF04695	0.573
LIG_Pex14_2	237	241	PF04695	0.447
LIG_Pex14_2	253	257	PF04695	0.447
LIG_Pex14_2	311	315	PF04695	0.368
LIG_SH2_CRK	281	285	PF00017	0.441
LIG_SH2_GRB2like	129	132	PF00017	0.743
LIG_SH2_GRB2like	42	45	PF00017	0.562
LIG_SH2_NCK_1	141	145	PF00017	0.705
LIG_SH2_PTP2	199	202	PF00017	0.480
LIG_SH2_PTP2	338	341	PF00017	0.625
LIG_SH2_SRC	139	142	PF00017	0.717
LIG_SH2_STAP1	141	145	PF00017	0.681
LIG_SH2_STAP1	29	33	PF00017	0.493
LIG_SH2_STAP1	366	370	PF00017	0.636
LIG_SH2_STAP1	56	60	PF00017	0.579
LIG_SH2_STAT3	287	290	PF00017	0.452
LIG_SH2_STAT5	199	202	PF00017	0.428
LIG_SH2_STAT5	325	328	PF00017	0.390
LIG_SH2_STAT5	338	341	PF00017	0.625
LIG_SH3_3	132	138	PF00018	0.673
LIG_SUMO_SIM_anti_2	172	177	PF11976	0.376
LIG_SUMO_SIM_par_1	199	204	PF11976	0.446
LIG_Vh1_VBS_1	170	188	PF01044	0.246
MOD_CK1_1	105	111	PF00069	0.636
MOD_CK1_1	161	167	PF00069	0.665
MOD_CK1_1	191	197	PF00069	0.394
MOD_CK1_1	227	233	PF00069	0.594
MOD_GlcNHglycan	126	129	PF01048	0.520
MOD_GlcNHglycan	292	295	PF01048	0.518
MOD_GSK3_1	188	195	PF00069	0.395
MOD_GSK3_1	227	234	PF00069	0.622
MOD_GSK3_1	309	316	PF00069	0.335
MOD_GSK3_1	348	355	PF00069	0.597
MOD_GSK3_1	98	105	PF00069	0.616
MOD_N-GLC_1	348	353	PF02516	0.343
MOD_N-GLC_1	63	68	PF02516	0.457
MOD_NEK2_1	123	128	PF00069	0.692
MOD_NEK2_1	183	188	PF00069	0.361
MOD_NEK2_1	192	197	PF00069	0.370
MOD_NEK2_1	201	206	PF00069	0.323
MOD_NEK2_1	235	240	PF00069	0.363
MOD_NEK2_1	309	314	PF00069	0.314
MOD_NEK2_1	348	353	PF00069	0.574
MOD_PIKK_1	376	382	PF00454	0.617
MOD_PK_1	65	71	PF00069	0.582
MOD_PKA_2	351	357	PF00069	0.597
MOD_Plk_1	348	354	PF00069	0.540
MOD_Plk_1	63	69	PF00069	0.647
MOD_Plk_2-3	158	164	PF00069	0.647
MOD_Plk_4	183	189	PF00069	0.379
MOD_Plk_4	192	198	PF00069	0.373
MOD_Plk_4	224	230	PF00069	0.592
MOD_Plk_4	231	237	PF00069	0.512
MOD_Plk_4	316	322	PF00069	0.368
MOD_Plk_4	354	360	PF00069	0.609
MOD_SUMO_for_1	92	95	PF00179	0.625
MOD_SUMO_rev_2	7	11	PF00179	0.615
TRG_DiLeu_BaEn_4	23	29	PF01217	0.567
TRG_ENDOCYTIC_2	199	202	PF00928	0.480
TRG_ENDOCYTIC_2	261	264	PF00928	0.302
TRG_ENDOCYTIC_2	281	284	PF00928	0.422
TRG_ENDOCYTIC_2	338	341	PF00928	0.625
TRG_ENDOCYTIC_2	50	53	PF00928	0.562
TRG_ER_diArg_1	212	215	PF00400	0.653
TRG_ER_diArg_1	247	250	PF00400	0.246
TRG_Pf-PMV_PEXEL_1	215	219	PF00026	0.415

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PGM1	Leptomonas seymouri	72%	100%
A0A0S4J3T8	Bodo saltans	30%	100%
A0A1X0P4V4	Trypanosomatidae	36%	100%
A0A3S7X9Z6	Leishmania donovani	93%	100%
A0A422P159	Trypanosoma rangeli	36%	100%
A4HNF2	Leishmania braziliensis	84%	100%
A4IC24	Leishmania infantum	93%	100%
C9ZYH5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	33%	100%
E9B716	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%