Membrane-trafficking protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

membrane- trafficking protein, putative

Species:

Leishmania major

UniProt:

E9AFV4_LEIMA

TriTrypDb:

LmjF.35.4790 , LMJLV39_350056000 , LMJSD75_350055300

Length:

220

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0010008	endosome membrane	5	2
GO:0012506	vesicle membrane	4	2
GO:0016020	membrane	2	12
GO:0030659	cytoplasmic vesicle membrane	5	2
GO:0031090	organelle membrane	3	2
GO:0032588	trans-Golgi network membrane	4	2
GO:0055038	recycling endosome membrane	6	2
GO:0098588	bounding membrane of organelle	4	2
GO:0110165	cellular anatomical entity	1	12

Expansion

Sequence features

E9AFV4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFV4

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	12
GO:0008104	protein localization	4	12
GO:0009987	cellular process	1	12
GO:0015031	protein transport	4	12
GO:0033036	macromolecule localization	2	12
GO:0045184	establishment of protein localization	3	12
GO:0051179	localization	1	12
GO:0051234	establishment of localization	2	12
GO:0051641	cellular localization	2	12
GO:0070727	cellular macromolecule localization	3	12
GO:0071702	organic substance transport	4	12
GO:0071705	nitrogen compound transport	4	12

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	170	172	PF00675	0.380
CLV_NRD_NRD_1	19	21	PF00675	0.368
CLV_PCSK_KEX2_1	19	21	PF00082	0.372
CLV_PCSK_KEX2_1	25	27	PF00082	0.392
CLV_PCSK_PC1ET2_1	25	27	PF00082	0.467
CLV_PCSK_SKI1_1	20	24	PF00082	0.454
CLV_PCSK_SKI1_1	205	209	PF00082	0.331
DOC_CYCLIN_RxL_1	199	211	PF00134	0.464
DOC_MAPK_gen_1	131	138	PF00069	0.468
DOC_MAPK_gen_1	171	179	PF00069	0.274
DOC_MAPK_gen_1	199	208	PF00069	0.476
DOC_MAPK_HePTP_8	169	181	PF00069	0.180
DOC_MAPK_MEF2A_6	171	179	PF00069	0.323
DOC_PP2B_LxvP_1	109	112	PF13499	0.318
DOC_USP7_MATH_1	129	133	PF00917	0.443
LIG_DCNL_PONY_1	1	4	PF03556	0.498
LIG_FHA_1	168	174	PF00498	0.247
LIG_FHA_1	209	215	PF00498	0.549
LIG_FHA_1	3	9	PF00498	0.471
LIG_FHA_1	74	80	PF00498	0.395
LIG_FHA_2	1	7	PF00498	0.615
LIG_LIR_Gen_1	104	112	PF02991	0.273
LIG_LIR_Gen_1	148	158	PF02991	0.273
LIG_LIR_Nem_3	104	108	PF02991	0.273
LIG_LIR_Nem_3	117	123	PF02991	0.273
LIG_LIR_Nem_3	148	153	PF02991	0.299
LIG_NRBOX	134	140	PF00104	0.384
LIG_PCNA_PIPBox_1	67	76	PF02747	0.488
LIG_PDZ_Class_1	215	220	PF00595	0.596
LIG_Pex14_1	115	119	PF04695	0.395
LIG_Pex14_2	101	105	PF04695	0.273
LIG_SH2_CRK	123	127	PF00017	0.464
LIG_SH2_CRK	52	56	PF00017	0.465
LIG_SH2_GRB2like	119	122	PF00017	0.446
LIG_SH2_GRB2like	52	55	PF00017	0.446
LIG_SH2_NCK_1	83	87	PF00017	0.459
LIG_SH2_PTP2	74	77	PF00017	0.352
LIG_SH2_STAP1	165	169	PF00017	0.305
LIG_SH2_STAT5	107	110	PF00017	0.272
LIG_SH2_STAT5	123	126	PF00017	0.478
LIG_SH2_STAT5	68	71	PF00017	0.436
LIG_SH2_STAT5	74	77	PF00017	0.266
LIG_SH3_3	35	41	PF00018	0.447
LIG_Sin3_3	176	183	PF02671	0.318
LIG_SUMO_SIM_anti_2	76	81	PF11976	0.343
LIG_SUMO_SIM_par_1	176	182	PF11976	0.353
LIG_TYR_ITIM	198	203	PF00017	0.505
LIG_TYR_ITIM	50	55	PF00017	0.530
MOD_CMANNOS	115	118	PF00535	0.401
MOD_GlcNHglycan	181	184	PF01048	0.360
MOD_GlcNHglycan	96	99	PF01048	0.450
MOD_GSK3_1	24	31	PF00069	0.666
MOD_N-GLC_1	28	33	PF02516	0.419
MOD_NEK2_1	101	106	PF00069	0.282
MOD_NEK2_1	167	172	PF00069	0.287
MOD_NEK2_1	208	213	PF00069	0.575
MOD_NEK2_1	73	78	PF00069	0.329
MOD_NEK2_1	94	99	PF00069	0.230
MOD_PIKK_1	24	30	PF00454	0.652
MOD_PIKK_1	59	65	PF00454	0.513
MOD_PKA_2	201	207	PF00069	0.545
MOD_Plk_1	28	34	PF00069	0.612
MOD_Plk_4	101	107	PF00069	0.281
MOD_Plk_4	73	79	PF00069	0.299
TRG_ENDOCYTIC_2	119	122	PF00928	0.458
TRG_ENDOCYTIC_2	123	126	PF00928	0.448
TRG_ENDOCYTIC_2	200	203	PF00928	0.496
TRG_ENDOCYTIC_2	52	55	PF00928	0.465
TRG_ENDOCYTIC_2	74	77	PF00928	0.296
TRG_NES_CRM1_1	134	148	PF08389	0.376

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P960	Leptomonas seymouri	65%	99%
A0A0S4J7Y6	Bodo saltans	37%	88%
A0A1X0P4W4	Trypanosomatidae	56%	97%
A0A3Q8IN09	Leishmania donovani	95%	100%
A0A3R7KQ76	Trypanosoma rangeli	57%	97%
A4HE02	Leishmania braziliensis	29%	91%
A4HNE3	Leishmania braziliensis	76%	100%
A4IC16	Leishmania infantum	95%	100%
C9ZYI6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	50%	97%
E9B707	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	100%
O15127	Homo sapiens	28%	67%
Q17QF8	Bos taurus	27%	94%
Q28F21	Xenopus tropicalis	27%	95%
Q58DF6	Bos taurus	26%	96%
Q5R5Z8	Pongo abelii	27%	94%
Q5RCY9	Pongo abelii	29%	96%
Q6GM42	Xenopus laevis	28%	94%
Q6GPA8	Xenopus laevis	29%	94%
Q6P0C7	Danio rerio	26%	96%
Q7F613	Oryza sativa subsp. japonica	27%	77%
Q8TAC9	Homo sapiens	27%	94%
Q969E2	Homo sapiens	29%	96%
Q9ERN0	Mus musculus	27%	67%
Q9ET20	Rattus norvegicus	27%	96%
Q9JKD3	Mus musculus	27%	94%
Q9JKE3	Rattus norvegicus	27%	94%
Q9JKV5	Mus musculus	26%	96%
Q9LR68	Arabidopsis thaliana	26%	78%