Putative small nuclear RNA gene activation protein (SNAP) 50

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

small nuclear RNA gene activation protein (SNAP) 50, putative

Species:

Leishmania major

UniProt:

E9AFU3_LEIMA

TriTrypDb:

LmjF.35.4660 , LMJLV39_350054900 , LMJSD75_350054200

Length:

430

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	12
GO:0005667	transcription regulator complex	2	2
GO:0019185	snRNA-activating protein complex	3	2
GO:0032991	protein-containing complex	1	2
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0043229	intracellular organelle	3	12
GO:0043231	intracellular membrane-bounded organelle	4	12
GO:0110165	cellular anatomical entity	1	12

Expansion

Sequence features

E9AFU3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFU3

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006351	DNA-templated transcription	7	2
GO:0006366	transcription by RNA polymerase II	8	2
GO:0006383	transcription by RNA polymerase III	8	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0009058	biosynthetic process	2	2
GO:0009059	macromolecule biosynthetic process	4	2
GO:0009301	snRNA transcription	8	2
GO:0009987	cellular process	1	2
GO:0016070	RNA metabolic process	5	2
GO:0016073	snRNA metabolic process	7	2
GO:0018130	heterocycle biosynthetic process	4	2
GO:0019438	aromatic compound biosynthetic process	4	2
GO:0032774	RNA biosynthetic process	5	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0034654	nucleobase-containing compound biosynthetic process	4	2
GO:0034660	ncRNA metabolic process	6	2
GO:0042795	snRNA transcription by RNA polymerase II	9	2
GO:0042796	snRNA transcription by RNA polymerase III	9	2
GO:0043170	macromolecule metabolic process	3	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044249	cellular biosynthetic process	3	2
GO:0044271	cellular nitrogen compound biosynthetic process	4	2
GO:0046483	heterocycle metabolic process	3	2
GO:0071704	organic substance metabolic process	2	2
GO:0090304	nucleic acid metabolic process	4	2
GO:0097659	nucleic acid-templated transcription	6	2
GO:0098781	ncRNA transcription	8	2
GO:1901360	organic cyclic compound metabolic process	3	2
GO:1901362	organic cyclic compound biosynthetic process	4	2
GO:1901576	organic substance biosynthetic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0000976	transcription cis-regulatory region binding	5	2
GO:0000987	cis-regulatory region sequence-specific DNA binding	6	2
GO:0000992	RNA polymerase III cis-regulatory region sequence-specific DNA binding	7	2
GO:0001006	RNA polymerase III type 3 promoter sequence-specific DNA binding	8	2
GO:0001016	RNA polymerase III transcription regulatory region sequence-specific DNA binding	6	2
GO:0001067	transcription regulatory region nucleic acid binding	4	2
GO:0003676	nucleic acid binding	3	12
GO:0003677	DNA binding	4	12
GO:0003681	bent DNA binding	5	2
GO:0003690	double-stranded DNA binding	5	2
GO:0005488	binding	1	12
GO:0043565	sequence-specific DNA binding	5	2
GO:0097159	organic cyclic compound binding	2	12
GO:1901363	heterocyclic compound binding	2	12
GO:1990837	sequence-specific double-stranded DNA binding	6	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	281	283	PF00675	0.371
CLV_NRD_NRD_1	348	350	PF00675	0.411
CLV_NRD_NRD_1	367	369	PF00675	0.403
CLV_NRD_NRD_1	4	6	PF00675	0.534
CLV_NRD_NRD_1	416	418	PF00675	0.577
CLV_NRD_NRD_1	95	97	PF00675	0.542
CLV_PCSK_KEX2_1	165	167	PF00082	0.426
CLV_PCSK_KEX2_1	192	194	PF00082	0.626
CLV_PCSK_KEX2_1	281	283	PF00082	0.371
CLV_PCSK_KEX2_1	367	369	PF00082	0.403
CLV_PCSK_KEX2_1	4	6	PF00082	0.532
CLV_PCSK_KEX2_1	416	418	PF00082	0.577
CLV_PCSK_PC1ET2_1	165	167	PF00082	0.426
CLV_PCSK_PC1ET2_1	192	194	PF00082	0.626
CLV_PCSK_PC7_1	363	369	PF00082	0.449
CLV_PCSK_SKI1_1	130	134	PF00082	0.491
CLV_PCSK_SKI1_1	251	255	PF00082	0.344
CLV_PCSK_SKI1_1	358	362	PF00082	0.342
CLV_PCSK_SKI1_1	368	372	PF00082	0.355
CLV_PCSK_SKI1_1	417	421	PF00082	0.555
CLV_PCSK_SKI1_1	96	100	PF00082	0.461
DEG_APCC_DBOX_1	367	375	PF00400	0.449
DEG_SPOP_SBC_1	246	250	PF00917	0.474
DOC_MAPK_gen_1	130	140	PF00069	0.538
DOC_MAPK_gen_1	40	47	PF00069	0.647
DOC_MAPK_gen_1	416	427	PF00069	0.627
DOC_MAPK_MEF2A_6	130	138	PF00069	0.597
DOC_MAPK_MEF2A_6	40	47	PF00069	0.626
DOC_USP7_MATH_1	145	149	PF00917	0.494
DOC_USP7_MATH_1	25	29	PF00917	0.575
DOC_USP7_MATH_1	304	308	PF00917	0.413
DOC_WW_Pin1_4	182	187	PF00397	0.639
DOC_WW_Pin1_4	384	389	PF00397	0.344
DOC_WW_Pin1_4	404	409	PF00397	0.145
DOC_WW_Pin1_4	49	54	PF00397	0.654
LIG_14-3-3_CanoR_1	266	274	PF00244	0.371
LIG_Actin_WH2_2	3	21	PF00022	0.591
LIG_APCC_ABBA_1	379	384	PF00400	0.449
LIG_BRCT_BRCA1_1	389	393	PF00533	0.449
LIG_Clathr_ClatBox_1	137	141	PF01394	0.412
LIG_CtBP_PxDLS_1	176	180	PF00389	0.496
LIG_FHA_1	100	106	PF00498	0.481
LIG_FHA_1	269	275	PF00498	0.296
LIG_FHA_1	376	382	PF00498	0.417
LIG_FHA_2	189	195	PF00498	0.518
LIG_FHA_2	240	246	PF00498	0.384
LIG_FHA_2	310	316	PF00498	0.274
LIG_FHA_2	405	411	PF00498	0.417
LIG_HP1_1	230	234	PF01393	0.474
LIG_LIR_Gen_1	221	231	PF02991	0.381
LIG_LIR_Gen_1	271	280	PF02991	0.345
LIG_LIR_Gen_1	290	300	PF02991	0.402
LIG_LIR_Nem_3	197	202	PF02991	0.586
LIG_LIR_Nem_3	221	226	PF02991	0.381
LIG_LIR_Nem_3	271	276	PF02991	0.345
LIG_LIR_Nem_3	290	295	PF02991	0.402
LIG_LIR_Nem_3	310	316	PF02991	0.346
LIG_LIR_Nem_3	333	338	PF02991	0.390
LIG_LIR_Nem_3	421	425	PF02991	0.563
LIG_OCRL_FandH_1	253	265	PF00620	0.436
LIG_Pex14_1	81	85	PF04695	0.600
LIG_SH2_CRK	199	203	PF00017	0.529
LIG_SH2_CRK	394	398	PF00017	0.328
LIG_SH2_SRC	382	385	PF00017	0.449
LIG_SH2_STAT5	199	202	PF00017	0.672
LIG_SH2_STAT5	262	265	PF00017	0.328
LIG_SH2_STAT5	335	338	PF00017	0.337
LIG_SH2_STAT5	394	397	PF00017	0.328
LIG_SH2_STAT5	426	429	PF00017	0.501
LIG_SH3_1	382	388	PF00018	0.449
LIG_SH3_3	31	37	PF00018	0.491
LIG_SH3_3	38	44	PF00018	0.453
LIG_SH3_3	382	388	PF00018	0.340
LIG_SUMO_SIM_par_1	134	139	PF11976	0.482
LIG_SUMO_SIM_par_1	175	181	PF11976	0.494
LIG_SUMO_SIM_par_1	230	236	PF11976	0.474
LIG_SUMO_SIM_par_1	377	384	PF11976	0.449
LIG_SUMO_SIM_par_1	43	48	PF11976	0.678
LIG_SUMO_SIM_par_1	9	14	PF11976	0.379
LIG_TRAF2_1	14	17	PF00917	0.578
LIG_TRAF2_1	149	152	PF00917	0.568
LIG_TRAF2_1	407	410	PF00917	0.453
LIG_WRC_WIRS_1	161	166	PF05994	0.635
LIG_WRC_WIRS_1	289	294	PF05994	0.248
LIG_WW_3	93	97	PF00397	0.622
MOD_CK1_1	178	184	PF00069	0.626
MOD_CK1_1	188	194	PF00069	0.495
MOD_CK1_1	387	393	PF00069	0.344
MOD_CK1_1	404	410	PF00069	0.344
MOD_CK1_1	76	82	PF00069	0.582
MOD_CK2_1	11	17	PF00069	0.508
MOD_CK2_1	145	151	PF00069	0.505
MOD_CK2_1	304	310	PF00069	0.428
MOD_CK2_1	404	410	PF00069	0.417
MOD_CK2_1	76	82	PF00069	0.582
MOD_CK2_1	97	103	PF00069	0.514
MOD_GlcNHglycan	147	150	PF01048	0.476
MOD_GlcNHglycan	306	309	PF01048	0.449
MOD_GlcNHglycan	344	347	PF01048	0.392
MOD_GlcNHglycan	61	64	PF01048	0.655
MOD_GSK3_1	178	185	PF00069	0.624
MOD_GSK3_1	304	311	PF00069	0.443
MOD_GSK3_1	336	343	PF00069	0.344
MOD_GSK3_1	73	80	PF00069	0.596
MOD_LATS_1	340	346	PF00433	0.474
MOD_NEK2_1	247	252	PF00069	0.435
MOD_NEK2_1	320	325	PF00069	0.433
MOD_NEK2_1	336	341	PF00069	0.218
MOD_NEK2_1	73	78	PF00069	0.587
MOD_NEK2_2	160	165	PF00069	0.633
MOD_NEK2_2	309	314	PF00069	0.423
MOD_PKB_1	266	274	PF00069	0.248
MOD_Plk_1	150	156	PF00069	0.593
MOD_Plk_1	287	293	PF00069	0.475
MOD_Plk_1	309	315	PF00069	0.324
MOD_Plk_4	73	79	PF00069	0.630
MOD_ProDKin_1	182	188	PF00069	0.634
MOD_ProDKin_1	384	390	PF00069	0.344
MOD_ProDKin_1	404	410	PF00069	0.145
MOD_ProDKin_1	49	55	PF00069	0.656
MOD_SUMO_for_1	118	121	PF00179	0.504
MOD_SUMO_for_1	164	167	PF00179	0.658
TRG_DiLeu_BaLyEn_6	132	137	PF01217	0.614
TRG_ENDOCYTIC_2	199	202	PF00928	0.672
TRG_ENDOCYTIC_2	335	338	PF00928	0.356
TRG_ENDOCYTIC_2	394	397	PF00928	0.433
TRG_ER_diArg_1	280	282	PF00400	0.371
TRG_ER_diArg_1	3	5	PF00400	0.527

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0S4JRS6	Bodo saltans	30%	89%
A0A1X0P603	Trypanosomatidae	41%	94%
A0A3Q8IJI9	Leishmania donovani	94%	100%
A0A3R7KX62	Trypanosoma rangeli	40%	97%
A4HND3	Leishmania braziliensis	90%	100%
A4IC05	Leishmania infantum	94%	100%
C9ZYK0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	38%	96%
E9B6Z5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	96%	100%
Q8IS08	Leptomonas seymouri	73%	90%
V5BCJ0	Trypanosoma cruzi	40%	97%