Protein-serine/threonine phosphatase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

kinetoplastid-specific dual specificity phosphatase, putative

Species:

Leishmania major

UniProt:

E9AFU2_LEIMA

TriTrypDb:

LmjF.35.4650 , LMJLV39_350054800 , LMJSD75_350054100

Length:

364

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 7
NetGPI	no	yes: 0, no: 7

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9AFU2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFU2

Function

Biological processes
Term	Name	Level	Count
GO:0006470	protein dephosphorylation	5	8
GO:0006793	phosphorus metabolic process	3	8
GO:0006796	phosphate-containing compound metabolic process	4	8
GO:0006807	nitrogen compound metabolic process	2	8
GO:0008152	metabolic process	1	8
GO:0009966	regulation of signal transduction	4	2
GO:0009968	negative regulation of signal transduction	5	2
GO:0009987	cellular process	1	8
GO:0010646	regulation of cell communication	4	2
GO:0010648	negative regulation of cell communication	5	2
GO:0016311	dephosphorylation	5	8
GO:0019538	protein metabolic process	3	8
GO:0023051	regulation of signaling	3	2
GO:0023057	negative regulation of signaling	4	2
GO:0036211	protein modification process	4	8
GO:0043170	macromolecule metabolic process	3	8
GO:0043408	regulation of MAPK cascade	6	2
GO:0043409	negative regulation of MAPK cascade	7	2
GO:0043412	macromolecule modification	4	8
GO:0044237	cellular metabolic process	2	8
GO:0044238	primary metabolic process	2	8
GO:0048519	negative regulation of biological process	3	2
GO:0048523	negative regulation of cellular process	4	2
GO:0048583	regulation of response to stimulus	3	2
GO:0048585	negative regulation of response to stimulus	4	2
GO:0050789	regulation of biological process	2	2
GO:0050794	regulation of cellular process	3	2
GO:0065007	biological regulation	1	2
GO:0071704	organic substance metabolic process	2	8
GO:1901564	organonitrogen compound metabolic process	3	8
GO:1902531	regulation of intracellular signal transduction	5	2
GO:1902532	negative regulation of intracellular signal transduction	6	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	8
GO:0004721	phosphoprotein phosphatase activity	3	8
GO:0008138	protein tyrosine/serine/threonine phosphatase activity	4	8
GO:0016787	hydrolase activity	2	8
GO:0016788	hydrolase activity, acting on ester bonds	3	8
GO:0016791	phosphatase activity	5	8
GO:0042578	phosphoric ester hydrolase activity	4	8
GO:0140096	catalytic activity, acting on a protein	2	8

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	116	120	PF00656	0.604
CLV_C14_Caspase3-7	188	192	PF00656	0.408
CLV_NRD_NRD_1	87	89	PF00675	0.680
CLV_PCSK_KEX2_1	87	89	PF00082	0.680
CLV_PCSK_SKI1_1	105	109	PF00082	0.735
CLV_PCSK_SKI1_1	87	91	PF00082	0.659
DEG_APCC_DBOX_1	315	323	PF00400	0.340
DEG_Nend_UBRbox_2	1	3	PF02207	0.623
DEG_SCF_FBW7_1	173	180	PF00400	0.494
DEG_SPOP_SBC_1	128	132	PF00917	0.764
DOC_CKS1_1	152	157	PF01111	0.576
DOC_CKS1_1	164	169	PF01111	0.517
DOC_CKS1_1	174	179	PF01111	0.438
DOC_MAPK_gen_1	326	336	PF00069	0.369
DOC_MAPK_gen_1	87	95	PF00069	0.611
DOC_MAPK_MEF2A_6	88	97	PF00069	0.614
DOC_USP7_MATH_1	115	119	PF00917	0.587
DOC_USP7_MATH_1	129	133	PF00917	0.633
DOC_USP7_MATH_1	48	52	PF00917	0.638
DOC_USP7_MATH_1	89	93	PF00917	0.635
DOC_WW_Pin1_4	121	126	PF00397	0.631
DOC_WW_Pin1_4	151	156	PF00397	0.579
DOC_WW_Pin1_4	163	168	PF00397	0.523
DOC_WW_Pin1_4	173	178	PF00397	0.447
DOC_WW_Pin1_4	4	9	PF00397	0.634
DOC_WW_Pin1_4	75	80	PF00397	0.595
DOC_WW_Pin1_4	82	87	PF00397	0.580
LIG_14-3-3_CanoR_1	127	135	PF00244	0.652
LIG_14-3-3_CanoR_1	187	191	PF00244	0.407
LIG_14-3-3_CanoR_1	316	320	PF00244	0.360
LIG_Actin_WH2_2	32	49	PF00022	0.513
LIG_APCC_ABBA_1	271	276	PF00400	0.351
LIG_BRCT_BRCA1_1	48	52	PF00533	0.675
LIG_FHA_1	152	158	PF00498	0.679
LIG_FHA_1	174	180	PF00498	0.493
LIG_FHA_1	208	214	PF00498	0.348
LIG_FHA_2	114	120	PF00498	0.684
LIG_FHA_2	186	192	PF00498	0.396
LIG_FHA_2	247	253	PF00498	0.351
LIG_FHA_2	284	290	PF00498	0.357
LIG_FHA_2	36	42	PF00498	0.608
LIG_FHA_2	70	76	PF00498	0.734
LIG_Integrin_isoDGR_2	331	333	PF01839	0.351
LIG_LIR_Apic_2	123	129	PF02991	0.648
LIG_LIR_Gen_1	267	277	PF02991	0.321
LIG_LIR_Nem_3	267	273	PF02991	0.321
LIG_LIR_Nem_3	276	280	PF02991	0.282
LIG_LIR_Nem_3	49	55	PF02991	0.725
LIG_PDZ_Class_3	359	364	PF00595	0.391
LIG_PTB_Apo_2	212	219	PF02174	0.389
LIG_PTB_Apo_2	336	343	PF02174	0.292
LIG_PTB_Phospho_1	212	218	PF10480	0.387
LIG_RPA_C_Fungi	299	311	PF08784	0.357
LIG_SH2_NCK_1	162	166	PF00017	0.551
LIG_SH2_STAP1	277	281	PF00017	0.292
LIG_SH2_STAT3	175	178	PF00017	0.545
LIG_SH2_STAT5	175	178	PF00017	0.545
LIG_SH2_STAT5	218	221	PF00017	0.351
LIG_SH2_STAT5	261	264	PF00017	0.363
LIG_SH2_STAT5	270	273	PF00017	0.325
LIG_SH2_STAT5	350	353	PF00017	0.381
LIG_SH3_2	83	88	PF14604	0.618
LIG_SH3_3	122	128	PF00018	0.648
LIG_SH3_3	161	167	PF00018	0.678
LIG_SH3_3	71	77	PF00018	0.587
LIG_SH3_3	80	86	PF00018	0.551
LIG_SUMO_SIM_par_1	71	78	PF11976	0.531
LIG_TRAF2_1	60	63	PF00917	0.677
LIG_Vh1_VBS_1	295	313	PF01044	0.248
MOD_CDC14_SPxK_1	78	81	PF00782	0.685
MOD_CDC14_SPxK_1	85	88	PF00782	0.601
MOD_CDK_SPK_2	82	87	PF00069	0.673
MOD_CDK_SPxK_1	121	127	PF00069	0.632
MOD_CDK_SPxK_1	75	81	PF00069	0.593
MOD_CDK_SPxK_1	82	88	PF00069	0.585
MOD_CK1_1	136	142	PF00069	0.608
MOD_CK1_1	208	214	PF00069	0.385
MOD_CK1_1	264	270	PF00069	0.357
MOD_CK1_1	58	64	PF00069	0.693
MOD_CK2_1	246	252	PF00069	0.307
MOD_CK2_1	283	289	PF00069	0.357
MOD_CK2_1	35	41	PF00069	0.605
MOD_CK2_1	69	75	PF00069	0.724
MOD_GlcNHglycan	10	13	PF01048	0.501
MOD_GlcNHglycan	131	134	PF01048	0.679
MOD_GlcNHglycan	135	138	PF01048	0.660
MOD_GlcNHglycan	197	200	PF01048	0.371
MOD_GSK3_1	109	116	PF00069	0.614
MOD_GSK3_1	129	136	PF00069	0.657
MOD_GSK3_1	173	180	PF00069	0.560
MOD_GSK3_1	4	11	PF00069	0.505
MOD_GSK3_1	42	49	PF00069	0.656
MOD_N-GLC_1	195	200	PF02516	0.365
MOD_N-GLC_1	302	307	PF02516	0.292
MOD_N-GLC_2	241	243	PF02516	0.292
MOD_NEK2_1	103	108	PF00069	0.622
MOD_NEK2_1	283	288	PF00069	0.357
MOD_NEK2_1	40	45	PF00069	0.683
MOD_NEK2_1	55	60	PF00069	0.674
MOD_NEK2_2	42	47	PF00069	0.584
MOD_PIKK_1	222	228	PF00454	0.357
MOD_PIKK_1	53	59	PF00454	0.679
MOD_PKA_2	186	192	PF00069	0.411
MOD_PKA_2	315	321	PF00069	0.340
MOD_PKA_2	46	52	PF00069	0.649
MOD_PKA_2	53	59	PF00069	0.697
MOD_PKA_2	64	70	PF00069	0.638
MOD_Plk_1	302	308	PF00069	0.292
MOD_Plk_1	35	41	PF00069	0.668
MOD_Plk_4	208	214	PF00069	0.419
MOD_Plk_4	283	289	PF00069	0.357
MOD_Plk_4	35	41	PF00069	0.517
MOD_Plk_4	69	75	PF00069	0.535
MOD_ProDKin_1	121	127	PF00069	0.632
MOD_ProDKin_1	151	157	PF00069	0.577
MOD_ProDKin_1	163	169	PF00069	0.521
MOD_ProDKin_1	173	179	PF00069	0.436
MOD_ProDKin_1	4	10	PF00069	0.632
MOD_ProDKin_1	75	81	PF00069	0.595
MOD_ProDKin_1	82	88	PF00069	0.583
TRG_DiLeu_BaEn_4	268	274	PF01217	0.357
TRG_ENDOCYTIC_2	270	273	PF00928	0.292
TRG_ENDOCYTIC_2	277	280	PF00928	0.292
TRG_ER_diArg_1	333	336	PF00400	0.351
TRG_ER_diArg_1	86	88	PF00400	0.683

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PES2	Leptomonas seymouri	56%	100%
A0A3Q8IJV8	Leishmania donovani	91%	100%
A4HND2	Leishmania braziliensis	75%	100%
A4IC04	Leishmania infantum	92%	100%
E9B6Z4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	100%
V5DUB4	Trypanosoma cruzi	37%	100%