J domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

chaperone protein DnaJ, putative

Species:

Leishmania major

UniProt:

E9AFU0_LEIMA

TriTrypDb:

LmjF.35.4630 , LMJLV39_350054600 , LMJSD75_350053900

Length:

491

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	10
GO:0031207	Sec62/Sec63 complex	3	2
GO:0032991	protein-containing complex	1	2
GO:0098796	membrane protein complex	2	2
GO:0110165	cellular anatomical entity	1	10
GO:0140534	endoplasmic reticulum protein-containing complex	2	2

Expansion

Sequence features

E9AFU0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFU0

Function

Biological processes
Term	Name	Level	Count
GO:0006605	protein targeting	5	2
GO:0006612	protein targeting to membrane	5	2
GO:0006613	cotranslational protein targeting to membrane	6	2
GO:0006614	SRP-dependent cotranslational protein targeting to membrane	7	2
GO:0006620	post-translational protein targeting to endoplasmic reticulum membrane	6	2
GO:0006810	transport	3	11
GO:0006886	intracellular protein transport	4	11
GO:0008104	protein localization	4	11
GO:0009987	cellular process	1	11
GO:0015031	protein transport	4	11
GO:0031204	post-translational protein targeting to membrane, translocation	5	11
GO:0033036	macromolecule localization	2	11
GO:0033365	protein localization to organelle	5	2
GO:0045047	protein targeting to ER	6	2
GO:0045184	establishment of protein localization	3	11
GO:0046907	intracellular transport	3	11
GO:0051179	localization	1	11
GO:0051234	establishment of localization	2	11
GO:0051641	cellular localization	2	11
GO:0051649	establishment of localization in cell	3	11
GO:0051668	localization within membrane	3	2
GO:0055085	transmembrane transport	2	11
GO:0065002	intracellular protein transmembrane transport	4	11
GO:0070727	cellular macromolecule localization	3	11
GO:0070972	protein localization to endoplasmic reticulum	6	2
GO:0071702	organic substance transport	4	11
GO:0071705	nitrogen compound transport	4	11
GO:0071806	protein transmembrane transport	3	11
GO:0072594	establishment of protein localization to organelle	4	2
GO:0072599	establishment of protein localization to endoplasmic reticulum	5	2
GO:0072657	protein localization to membrane	4	2
GO:0090150	establishment of protein localization to membrane	4	2

Molecular functions
Term	Name	Level	Count
GO:0005215	transporter activity	1	2
GO:0008320	protein transmembrane transporter activity	3	2
GO:0022857	transmembrane transporter activity	2	2
GO:0022884	macromolecule transmembrane transporter activity	3	2
GO:0140318	protein transporter activity	2	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	452	456	PF00656	0.563
CLV_MEL_PAP_1	446	452	PF00089	0.370
CLV_NRD_NRD_1	150	152	PF00675	0.571
CLV_NRD_NRD_1	179	181	PF00675	0.495
CLV_NRD_NRD_1	278	280	PF00675	0.344
CLV_NRD_NRD_1	282	284	PF00675	0.324
CLV_NRD_NRD_1	38	40	PF00675	0.433
CLV_PCSK_KEX2_1	159	161	PF00082	0.496
CLV_PCSK_KEX2_1	179	181	PF00082	0.350
CLV_PCSK_KEX2_1	278	280	PF00082	0.360
CLV_PCSK_KEX2_1	282	284	PF00082	0.324
CLV_PCSK_KEX2_1	38	40	PF00082	0.406
CLV_PCSK_PC1ET2_1	159	161	PF00082	0.513
CLV_PCSK_PC7_1	155	161	PF00082	0.496
CLV_PCSK_PC7_1	278	284	PF00082	0.354
CLV_PCSK_SKI1_1	103	107	PF00082	0.373
CLV_PCSK_SKI1_1	112	116	PF00082	0.335
CLV_PCSK_SKI1_1	188	192	PF00082	0.593
CLV_PCSK_SKI1_1	210	214	PF00082	0.599
CLV_PCSK_SKI1_1	283	287	PF00082	0.400
CLV_PCSK_SKI1_1	383	387	PF00082	0.344
CLV_PCSK_SKI1_1	39	43	PF00082	0.435
CLV_PCSK_SKI1_1	412	416	PF00082	0.403
CLV_PCSK_SKI1_1	93	97	PF00082	0.355
DEG_APCC_DBOX_1	111	119	PF00400	0.546
DEG_APCC_DBOX_1	282	290	PF00400	0.568
DOC_CYCLIN_RxL_1	322	333	PF00134	0.628
DOC_CYCLIN_RxL_1	35	46	PF00134	0.661
DOC_CYCLIN_RxL_1	380	390	PF00134	0.553
DOC_CYCLIN_RxL_1	410	420	PF00134	0.635
DOC_CYCLIN_yClb5_NLxxxL_5	413	421	PF00134	0.606
DOC_CYCLIN_yCln2_LP_2	28	34	PF00134	0.378
DOC_MAPK_DCC_7	110	120	PF00069	0.558
DOC_MAPK_gen_1	110	117	PF00069	0.465
DOC_MAPK_gen_1	244	255	PF00069	0.621
DOC_MAPK_gen_1	278	288	PF00069	0.513
DOC_MAPK_gen_1	434	443	PF00069	0.646
DOC_MAPK_MEF2A_6	106	115	PF00069	0.578
DOC_MAPK_MEF2A_6	282	290	PF00069	0.517
DOC_MAPK_MEF2A_6	395	404	PF00069	0.666
DOC_PP1_RVXF_1	101	108	PF00149	0.571
DOC_PP1_RVXF_1	246	252	PF00149	0.579
DOC_PP1_RVXF_1	91	97	PF00149	0.553
DOC_PP2B_LxvP_1	139	142	PF13499	0.334
DOC_PP2B_LxvP_1	28	31	PF13499	0.378
DOC_PP4_FxxP_1	267	270	PF00568	0.568
DOC_USP7_MATH_1	19	23	PF00917	0.335
DOC_USP7_MATH_1	339	343	PF00917	0.553
DOC_USP7_MATH_1	360	364	PF00917	0.682
DOC_WW_Pin1_4	53	58	PF00397	0.615
LIG_14-3-3_CanoR_1	151	155	PF00244	0.330
LIG_14-3-3_CanoR_1	449	457	PF00244	0.595
LIG_Actin_WH2_2	90	108	PF00022	0.568
LIG_APCC_ABBA_1	415	420	PF00400	0.558
LIG_BIR_II_1	1	5	PF00653	0.489
LIG_BRCT_BRCA1_1	129	133	PF00533	0.585
LIG_BRCT_BRCA1_1	14	18	PF00533	0.355
LIG_FHA_1	145	151	PF00498	0.282
LIG_FHA_1	19	25	PF00498	0.432
LIG_FHA_1	380	386	PF00498	0.563
LIG_FHA_1	388	394	PF00498	0.532
LIG_FHA_2	196	202	PF00498	0.490
LIG_FHA_2	211	217	PF00498	0.357
LIG_FHA_2	45	51	PF00498	0.653
LIG_LIR_Gen_1	10	20	PF02991	0.282
LIG_LIR_Gen_1	125	135	PF02991	0.461
LIG_LIR_Gen_1	173	182	PF02991	0.313
LIG_LIR_Gen_1	342	351	PF02991	0.642
LIG_LIR_Gen_1	369	379	PF02991	0.627
LIG_LIR_Gen_1	419	430	PF02991	0.541
LIG_LIR_Nem_3	10	16	PF02991	0.282
LIG_LIR_Nem_3	125	131	PF02991	0.335
LIG_LIR_Nem_3	153	157	PF02991	0.362
LIG_LIR_Nem_3	173	178	PF02991	0.150
LIG_LIR_Nem_3	342	346	PF02991	0.654
LIG_LIR_Nem_3	369	374	PF02991	0.592
LIG_LIR_Nem_3	419	425	PF02991	0.535
LIG_NRBOX	221	227	PF00104	0.335
LIG_NRBOX	420	426	PF00104	0.596
LIG_Pex14_1	119	123	PF04695	0.355
LIG_Pex14_1	251	255	PF04695	0.559
LIG_Pex14_2	263	267	PF04695	0.568
LIG_REV1ctd_RIR_1	93	101	PF16727	0.555
LIG_SH2_CRK	128	132	PF00017	0.525
LIG_SH2_CRK	154	158	PF00017	0.295
LIG_SH2_CRK	182	186	PF00017	0.282
LIG_SH2_CRK	343	347	PF00017	0.606
LIG_SH2_GRB2like	472	475	PF00017	0.647
LIG_SH2_NCK_1	355	359	PF00017	0.619
LIG_SH2_NCK_1	73	77	PF00017	0.650
LIG_SH2_PTP2	175	178	PF00017	0.296
LIG_SH2_STAT3	384	387	PF00017	0.608
LIG_SH2_STAT3	472	475	PF00017	0.582
LIG_SH2_STAT5	175	178	PF00017	0.282
LIG_SH2_STAT5	235	238	PF00017	0.482
LIG_SH2_STAT5	275	278	PF00017	0.554
LIG_SH2_STAT5	29	32	PF00017	0.335
LIG_SH2_STAT5	384	387	PF00017	0.521
LIG_SH2_STAT5	429	432	PF00017	0.638
LIG_SH2_STAT5	67	70	PF00017	0.582
LIG_SH3_3	105	111	PF00018	0.661
LIG_SH3_3	203	209	PF00018	0.463
LIG_SH3_3	267	273	PF00018	0.545
LIG_SH3_4	434	441	PF00018	0.618
LIG_SUMO_SIM_anti_2	21	28	PF11976	0.358
LIG_SUMO_SIM_par_1	288	293	PF11976	0.519
LIG_TRAF2_1	190	193	PF00917	0.386
LIG_TRAF2_1	198	201	PF00917	0.427
LIG_TRAF2_1	396	399	PF00917	0.620
LIG_UBA3_1	249	257	PF00899	0.627
LIG_UBA3_1	326	335	PF00899	0.656
LIG_WRC_WIRS_1	13	18	PF05994	0.355
MOD_CK1_1	143	149	PF00069	0.328
MOD_CK1_1	53	59	PF00069	0.613
MOD_CK1_1	9	15	PF00069	0.403
MOD_CK2_1	143	149	PF00069	0.280
MOD_CK2_1	195	201	PF00069	0.492
MOD_CK2_1	210	216	PF00069	0.357
MOD_CK2_1	44	50	PF00069	0.624
MOD_GlcNHglycan	124	127	PF01048	0.355
MOD_GlcNHglycan	143	146	PF01048	0.496
MOD_GlcNHglycan	376	379	PF01048	0.380
MOD_GlcNHglycan	409	412	PF01048	0.375
MOD_GlcNHglycan	63	66	PF01048	0.449
MOD_GSK3_1	140	147	PF00069	0.310
MOD_N-GLC_1	165	170	PF02516	0.482
MOD_NEK2_1	122	127	PF00069	0.351
MOD_NEK2_1	18	23	PF00069	0.430
MOD_NEK2_1	61	66	PF00069	0.583
MOD_PK_1	442	448	PF00069	0.465
MOD_PKA_1	278	284	PF00069	0.538
MOD_PKA_2	150	156	PF00069	0.378
MOD_PKA_2	278	284	PF00069	0.538
MOD_PKA_2	379	385	PF00069	0.600
MOD_PKA_2	448	454	PF00069	0.601
MOD_Plk_1	200	206	PF00069	0.420
MOD_Plk_1	9	15	PF00069	0.403
MOD_Plk_2-3	195	201	PF00069	0.467
MOD_Plk_4	19	25	PF00069	0.335
MOD_Plk_4	290	296	PF00069	0.567
MOD_Plk_4	339	345	PF00069	0.612
MOD_Plk_4	63	69	PF00069	0.560
MOD_Plk_4	78	84	PF00069	0.519
MOD_Plk_4	9	15	PF00069	0.411
MOD_ProDKin_1	53	59	PF00069	0.615
MOD_SUMO_rev_2	189	198	PF00179	0.360
MOD_SUMO_rev_2	363	368	PF00179	0.648
TRG_DiLeu_BaEn_1	216	221	PF01217	0.428
TRG_DiLeu_BaEn_1	420	425	PF01217	0.512
TRG_DiLeu_BaEn_2	91	97	PF01217	0.590
TRG_DiLeu_BaLyEn_6	245	250	PF01217	0.620
TRG_DiLeu_BaLyEn_6	306	311	PF01217	0.468
TRG_ENDOCYTIC_2	128	131	PF00928	0.482
TRG_ENDOCYTIC_2	154	157	PF00928	0.295
TRG_ENDOCYTIC_2	175	178	PF00928	0.282
TRG_ENDOCYTIC_2	182	185	PF00928	0.282
TRG_ENDOCYTIC_2	343	346	PF00928	0.608
TRG_ENDOCYTIC_2	422	425	PF00928	0.569
TRG_ER_diArg_1	109	112	PF00400	0.665
TRG_ER_diArg_1	178	180	PF00400	0.293
TRG_ER_diArg_1	38	40	PF00400	0.622
TRG_NES_CRM1_1	419	433	PF08389	0.595
TRG_Pf-PMV_PEXEL_1	172	177	PF00026	0.549
TRG_Pf-PMV_PEXEL_1	283	287	PF00026	0.340
TRG_Pf-PMV_PEXEL_1	328	333	PF00026	0.435
TRG_Pf-PMV_PEXEL_1	383	387	PF00026	0.335
TRG_Pf-PMV_PEXEL_1	395	399	PF00026	0.329

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1ICG5	Leptomonas seymouri	80%	100%
A0A0S4JR42	Bodo saltans	48%	99%
A0A1X0P4Z0	Trypanosomatidae	61%	100%
A0A3R7RRP5	Trypanosoma rangeli	58%	100%
A0A3S7X9X2	Leishmania donovani	96%	100%
A4HND0	Leishmania braziliensis	88%	100%
A4IC02	Leishmania infantum	97%	100%
C9ZYK3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	50%	100%
E9B6Z2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	96%	100%