Roadblock/LC7 domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania major

UniProt:

E9AFT4_LEIMA

TriTrypDb:

LmjF.35.4570 , LMJLV39_350054000 , LMJSD75_350053300

Length:

140

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0032045	guanyl-nucleotide exchange factor complex	3	2
GO:0032991	protein-containing complex	1	2
GO:0071986	Ragulator complex	3	2
GO:0098796	membrane protein complex	2	2
GO:0140535	intracellular protein-containing complex	2	2

Expansion

Sequence features

E9AFT4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFT4

Function

Biological processes
Term	Name	Level	Count
GO:0001101	response to acid chemical	3	2
GO:0009719	response to endogenous stimulus	2	2
GO:0009966	regulation of signal transduction	4	12
GO:0009967	positive regulation of signal transduction	5	12
GO:0009987	cellular process	1	2
GO:0010033	response to organic substance	3	2
GO:0010243	response to organonitrogen compound	4	2
GO:0010646	regulation of cell communication	4	12
GO:0010647	positive regulation of cell communication	5	12
GO:0023051	regulation of signaling	3	12
GO:0023056	positive regulation of signaling	4	12
GO:0032006	regulation of TOR signaling	6	12
GO:0032008	positive regulation of TOR signaling	7	12
GO:0042221	response to chemical	2	2
GO:0043200	response to amino acid	4	2
GO:0048518	positive regulation of biological process	3	12
GO:0048522	positive regulation of cellular process	4	12
GO:0048583	regulation of response to stimulus	3	12
GO:0048584	positive regulation of response to stimulus	4	12
GO:0050789	regulation of biological process	2	12
GO:0050794	regulation of cellular process	3	12
GO:0050896	response to stimulus	1	2
GO:0051716	cellular response to stimulus	2	2
GO:0065007	biological regulation	1	12
GO:0070887	cellular response to chemical stimulus	3	2
GO:0071229	cellular response to acid chemical	4	2
GO:0071230	cellular response to amino acid stimulus	5	2
GO:0071310	cellular response to organic substance	4	2
GO:0071417	cellular response to organonitrogen compound	4	2
GO:0071495	cellular response to endogenous stimulus	3	2
GO:1901698	response to nitrogen compound	3	2
GO:1901699	cellular response to nitrogen compound	4	2
GO:1901700	response to oxygen-containing compound	3	2
GO:1901701	cellular response to oxygen-containing compound	4	2
GO:1902531	regulation of intracellular signal transduction	5	12
GO:1902533	positive regulation of intracellular signal transduction	6	12

Molecular functions
Term	Name	Level	Count
GO:0005085	guanyl-nucleotide exchange factor activity	3	12
GO:0030234	enzyme regulator activity	2	12
GO:0030695	GTPase regulator activity	4	12
GO:0060090	molecular adaptor activity	1	12
GO:0060589	nucleoside-triphosphatase regulator activity	3	12
GO:0098772	molecular function regulator activity	1	12
GO:0140677	molecular function activator activity	2	12
GO:0140678	molecular function inhibitor activity	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	127	129	PF00675	0.421
DEG_Nend_UBRbox_1	1	4	PF02207	0.362
DOC_CYCLIN_yCln2_LP_2	133	139	PF00134	0.517
DOC_MAPK_gen_1	132	140	PF00069	0.516
DOC_MAPK_gen_1	92	99	PF00069	0.388
DOC_USP7_MATH_1	103	107	PF00917	0.456
LIG_Actin_WH2_2	107	124	PF00022	0.410
LIG_APCC_ABBA_1	86	91	PF00400	0.512
LIG_BIR_III_4	28	32	PF00653	0.522
LIG_Clathr_ClatBox_1	107	111	PF01394	0.489
LIG_FHA_1	100	106	PF00498	0.493
LIG_FHA_1	121	127	PF00498	0.392
LIG_FHA_1	77	83	PF00498	0.502
LIG_LIR_Gen_1	115	126	PF02991	0.473
LIG_LIR_Nem_3	115	121	PF02991	0.488
LIG_LIR_Nem_3	46	52	PF02991	0.537
LIG_NRBOX	129	135	PF00104	0.512
LIG_NRBOX	21	27	PF00104	0.460
LIG_PCNA_yPIPBox_3	120	134	PF02747	0.472
LIG_SH2_CRK	49	53	PF00017	0.535
LIG_SH2_SRC	49	52	PF00017	0.502
LIG_SH3_3	84	90	PF00018	0.503
LIG_SUMO_SIM_par_1	101	109	PF11976	0.477
LIG_SUMO_SIM_par_1	22	28	PF11976	0.357
LIG_TYR_ITIM	47	52	PF00017	0.390
LIG_UBA3_1	85	93	PF00899	0.459
MOD_CK1_1	106	112	PF00069	0.486
MOD_CK2_1	110	116	PF00069	0.476
MOD_GlcNHglycan	14	17	PF01048	0.437
MOD_GlcNHglycan	28	32	PF01048	0.342
MOD_GSK3_1	106	113	PF00069	0.407
MOD_GSK3_1	95	102	PF00069	0.420
MOD_NEK2_1	27	32	PF00069	0.409
MOD_NEK2_1	38	43	PF00069	0.334
MOD_Plk_1	110	116	PF00069	0.542
MOD_Plk_2-3	78	84	PF00069	0.508
MOD_Plk_4	103	109	PF00069	0.457
TRG_DiLeu_BaLyEn_6	129	134	PF01217	0.455
TRG_ENDOCYTIC_2	49	52	PF00928	0.496

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P966	Leptomonas seymouri	81%	100%
A0A0S4JTI2	Bodo saltans	39%	100%
A0A1X0P543	Trypanosomatidae	45%	100%
A0A3Q8IIR8	Leishmania donovani	96%	100%
A0A422P194	Trypanosoma rangeli	42%	100%
A4HNC4	Leishmania braziliensis	91%	100%
A4IBZ7	Leishmania infantum	96%	100%
C9ZYK7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	100%
E9B6Y6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	100%
V5BCJ6	Trypanosoma cruzi	47%	81%