Uncharacterized protein

Quick info Localization Expansion Sequence features Structure Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania major

UniProt:

E9AFR1_LEIMA

TriTrypDb:

LmjF.35.4340 , LMJLV39_350051500 , LMJSD75_350050900

Length:

342

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 1, no: 10
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	8
GO:0110165	cellular anatomical entity	1	8

Expansion

Sequence features

E9AFR1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFR1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	54	58	PF00656	0.368
CLV_NRD_NRD_1	136	138	PF00675	0.540
CLV_NRD_NRD_1	142	144	PF00675	0.557
CLV_NRD_NRD_1	171	173	PF00675	0.531
CLV_NRD_NRD_1	185	187	PF00675	0.513
CLV_NRD_NRD_1	204	206	PF00675	0.616
CLV_NRD_NRD_1	42	44	PF00675	0.631
CLV_NRD_NRD_1	87	89	PF00675	0.659
CLV_NRD_NRD_1	96	98	PF00675	0.619
CLV_PCSK_KEX2_1	136	138	PF00082	0.531
CLV_PCSK_KEX2_1	171	173	PF00082	0.536
CLV_PCSK_KEX2_1	185	187	PF00082	0.513
CLV_PCSK_KEX2_1	204	206	PF00082	0.546
CLV_PCSK_KEX2_1	95	97	PF00082	0.647
CLV_PCSK_SKI1_1	164	168	PF00082	0.537
CLV_PCSK_SKI1_1	171	175	PF00082	0.486
CLV_PCSK_SKI1_1	204	208	PF00082	0.559
CLV_PCSK_SKI1_1	230	234	PF00082	0.613
CLV_PCSK_SKI1_1	37	41	PF00082	0.589
DEG_Nend_Nbox_1	1	3	PF02207	0.498
DOC_CDC14_PxL_1	46	54	PF14671	0.419
DOC_CKS1_1	268	273	PF01111	0.443
DOC_CYCLIN_RxL_1	93	102	PF00134	0.433
DOC_MAPK_gen_1	201	211	PF00069	0.267
DOC_MAPK_HePTP_8	232	244	PF00069	0.279
DOC_MAPK_MEF2A_6	204	211	PF00069	0.267
DOC_MAPK_MEF2A_6	235	244	PF00069	0.286
DOC_PP2B_LxvP_1	190	193	PF13499	0.344
DOC_USP7_UBL2_3	326	330	PF12436	0.660
DOC_USP7_UBL2_3	73	77	PF12436	0.270
DOC_WW_Pin1_4	153	158	PF00397	0.436
DOC_WW_Pin1_4	267	272	PF00397	0.468
LIG_14-3-3_CanoR_1	112	117	PF00244	0.386
LIG_14-3-3_CanoR_1	3	10	PF00244	0.476
LIG_14-3-3_CanoR_1	43	47	PF00244	0.459
LIG_Actin_WH2_2	52	69	PF00022	0.377
LIG_BIR_III_4	57	61	PF00653	0.370
LIG_Clathr_ClatBox_1	166	170	PF01394	0.303
LIG_FHA_1	140	146	PF00498	0.350
LIG_FHA_1	229	235	PF00498	0.411
LIG_FHA_1	255	261	PF00498	0.387
LIG_FHA_1	315	321	PF00498	0.609
LIG_FHA_1	33	39	PF00498	0.421
LIG_FHA_2	115	121	PF00498	0.450
LIG_FHA_2	146	152	PF00498	0.384
LIG_FHA_2	153	159	PF00498	0.424
LIG_FHA_2	197	203	PF00498	0.337
LIG_FHA_2	217	223	PF00498	0.427
LIG_FHA_2	241	247	PF00498	0.425
LIG_FHA_2	268	274	PF00498	0.473
LIG_LIR_Gen_1	273	283	PF02991	0.371
LIG_LIR_Nem_3	226	232	PF02991	0.381
LIG_LIR_Nem_3	273	278	PF02991	0.375
LIG_LIR_Nem_3	295	299	PF02991	0.350
LIG_LIR_Nem_3	45	49	PF02991	0.414
LIG_LYPXL_yS_3	49	52	PF13949	0.433
LIG_NRBOX	162	168	PF00104	0.338
LIG_Pex14_1	286	290	PF04695	0.334
LIG_Pex14_1	293	297	PF04695	0.321
LIG_Pex14_2	311	315	PF04695	0.613
LIG_SH2_STAP1	110	114	PF00017	0.383
LIG_SH2_STAT5	126	129	PF00017	0.374
LIG_SH2_STAT5	332	335	PF00017	0.650
LIG_SH3_3	19	25	PF00018	0.374
LIG_SH3_3	262	268	PF00018	0.378
LIG_SUMO_SIM_anti_2	165	170	PF11976	0.331
LIG_SUMO_SIM_anti_2	208	213	PF11976	0.380
LIG_SUMO_SIM_anti_2	300	308	PF11976	0.395
LIG_SUMO_SIM_par_1	111	117	PF11976	0.426
LIG_SUMO_SIM_par_1	165	170	PF11976	0.344
LIG_SUMO_SIM_par_1	205	210	PF11976	0.379
LIG_TRAF2_1	236	239	PF00917	0.425
LIG_TRAF2_1	39	42	PF00917	0.438
LIG_TYR_ITIM	283	288	PF00017	0.287
LIG_UBA3_1	12	21	PF00899	0.400
LIG_UBA3_1	206	214	PF00899	0.370
LIG_UBA3_1	259	267	PF00899	0.419
LIG_UBA3_1	309	313	PF00899	0.467
MOD_CK2_1	114	120	PF00069	0.464
MOD_CK2_1	145	151	PF00069	0.367
MOD_CK2_1	152	158	PF00069	0.441
MOD_CK2_1	196	202	PF00069	0.408
MOD_CK2_1	216	222	PF00069	0.426
MOD_CK2_1	240	246	PF00069	0.371
MOD_CMANNOS	286	289	PF00535	0.344
MOD_Cter_Amidation	183	186	PF01082	0.616
MOD_GlcNHglycan	13	16	PF01048	0.603
MOD_GlcNHglycan	28	31	PF01048	0.593
MOD_GlcNHglycan	334	337	PF01048	0.499
MOD_GlcNHglycan	63	66	PF01048	0.636
MOD_GSK3_1	152	159	PF00069	0.415
MOD_GSK3_1	192	199	PF00069	0.399
MOD_N-GLC_1	216	221	PF02516	0.612
MOD_NEK2_1	2	7	PF00069	0.468
MOD_NEK2_1	207	212	PF00069	0.429
MOD_NEK2_1	228	233	PF00069	0.429
MOD_NEK2_1	278	283	PF00069	0.409
MOD_NEK2_2	292	297	PF00069	0.475
MOD_PIKK_1	114	120	PF00454	0.445
MOD_PIKK_1	177	183	PF00454	0.424
MOD_PK_1	112	118	PF00069	0.396
MOD_PKA_1	136	142	PF00069	0.350
MOD_PKA_1	171	177	PF00069	0.423
MOD_PKA_1	73	79	PF00069	0.406
MOD_PKA_1	88	94	PF00069	0.411
MOD_PKA_2	136	142	PF00069	0.366
MOD_PKA_2	171	177	PF00069	0.383
MOD_PKA_2	2	8	PF00069	0.490
MOD_PKA_2	254	260	PF00069	0.368
MOD_PKA_2	278	284	PF00069	0.435
MOD_PKA_2	42	48	PF00069	0.459
MOD_Plk_1	207	213	PF00069	0.438
MOD_Plk_1	216	222	PF00069	0.416
MOD_Plk_2-3	196	202	PF00069	0.351
MOD_Plk_2-3	42	48	PF00069	0.397
MOD_Plk_4	136	142	PF00069	0.363
MOD_Plk_4	278	284	PF00069	0.365
MOD_Plk_4	292	298	PF00069	0.341
MOD_Plk_4	42	48	PF00069	0.397
MOD_ProDKin_1	153	159	PF00069	0.426
MOD_ProDKin_1	267	273	PF00069	0.467
MOD_SUMO_for_1	197	200	PF00179	0.362
MOD_SUMO_rev_2	147	156	PF00179	0.415
MOD_SUMO_rev_2	195	199	PF00179	0.373
MOD_SUMO_rev_2	41	45	PF00179	0.441
TRG_DiLeu_BaEn_1	158	163	PF01217	0.379
TRG_DiLeu_BaEn_1	202	207	PF01217	0.382
TRG_DiLeu_BaEn_1	302	307	PF01217	0.475
TRG_DiLeu_LyEn_5	202	207	PF01217	0.407
TRG_ENDOCYTIC_2	110	113	PF00928	0.375
TRG_ENDOCYTIC_2	285	288	PF00928	0.344
TRG_ENDOCYTIC_2	49	52	PF00928	0.418
TRG_ER_diArg_1	135	137	PF00400	0.337
TRG_ER_diArg_1	171	173	PF00400	0.336
TRG_ER_diArg_1	185	187	PF00400	0.313
TRG_ER_diArg_1	204	206	PF00400	0.264
TRG_ER_diArg_1	94	97	PF00400	0.427
TRG_NES_CRM1_1	57	70	PF08389	0.273
TRG_Pf-PMV_PEXEL_1	204	208	PF00026	0.637
TRG_Pf-PMV_PEXEL_1	318	322	PF00026	0.408

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P542	Leptomonas seymouri	26%	88%
A0A0N1I4W7	Leptomonas seymouri	74%	100%
A0A0S4JN93	Bodo saltans	46%	100%
A0A1X0P510	Trypanosomatidae	52%	99%
A0A3Q8IVJ8	Leishmania donovani	96%	100%
A4HNA3	Leishmania braziliensis	87%	100%
A4IBX4	Leishmania infantum	96%	100%
C9ZYN3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	52%	84%
E9B6W4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	96%	100%
V5BXU8	Trypanosoma cruzi	52%	99%