RNA polymerase III RPC4 family protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

RNA polymerase III RPC4, putative

Species:

Leishmania major

UniProt:

E9AFP4_LEIMA

TriTrypDb:

LmjF.35.4170 , LMJLV39_350049700 , LMJSD75_350049100

Length:

364

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 8
NetGPI	no	yes: 0, no: 8

Cellular components
Term	Name	Level	Count
GO:0000428	DNA-directed RNA polymerase complex	4	7
GO:0005666	RNA polymerase III complex	4	7
GO:0030880	RNA polymerase complex	3	7
GO:0032991	protein-containing complex	1	7
GO:0055029	nuclear DNA-directed RNA polymerase complex	3	7
GO:0061695	transferase complex, transferring phosphorus-containing groups	4	7
GO:0140513	nuclear protein-containing complex	2	7
GO:0140535	intracellular protein-containing complex	2	7
GO:1902494	catalytic complex	2	7
GO:1990234	transferase complex	3	7

Expansion

Sequence features

E9AFP4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFP4

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	7
GO:0006351	DNA-templated transcription	7	7
GO:0006383	transcription by RNA polymerase III	8	7
GO:0006725	cellular aromatic compound metabolic process	3	7
GO:0006807	nitrogen compound metabolic process	2	7
GO:0008152	metabolic process	1	7
GO:0009058	biosynthetic process	2	7
GO:0009059	macromolecule biosynthetic process	4	7
GO:0009987	cellular process	1	7
GO:0016070	RNA metabolic process	5	7
GO:0018130	heterocycle biosynthetic process	4	7
GO:0019438	aromatic compound biosynthetic process	4	7
GO:0032774	RNA biosynthetic process	5	7
GO:0034641	cellular nitrogen compound metabolic process	3	7
GO:0034654	nucleobase-containing compound biosynthetic process	4	7
GO:0043170	macromolecule metabolic process	3	7
GO:0044237	cellular metabolic process	2	7
GO:0044238	primary metabolic process	2	7
GO:0044249	cellular biosynthetic process	3	7
GO:0044271	cellular nitrogen compound biosynthetic process	4	7
GO:0046483	heterocycle metabolic process	3	7
GO:0071704	organic substance metabolic process	2	7
GO:0090304	nucleic acid metabolic process	4	7
GO:0097659	nucleic acid-templated transcription	6	7
GO:1901360	organic cyclic compound metabolic process	3	7
GO:1901362	organic cyclic compound biosynthetic process	4	7
GO:1901576	organic substance biosynthetic process	3	7

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	7
GO:0003677	DNA binding	4	7
GO:0005488	binding	1	7
GO:0097159	organic cyclic compound binding	2	7
GO:1901363	heterocyclic compound binding	2	7

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	138	140	PF00675	0.667
CLV_PCSK_SKI1_1	13	17	PF00082	0.722
CLV_PCSK_SKI1_1	197	201	PF00082	0.556
CLV_PCSK_SKI1_1	300	304	PF00082	0.457
DEG_SPOP_SBC_1	51	55	PF00917	0.705
DEG_SPOP_SBC_1	67	71	PF00917	0.747
DOC_CKS1_1	358	363	PF01111	0.357
DOC_CYCLIN_yCln2_LP_2	178	184	PF00134	0.687
DOC_MAPK_gen_1	294	303	PF00069	0.371
DOC_MAPK_gen_1	9	16	PF00069	0.709
DOC_MAPK_MEF2A_6	297	305	PF00069	0.492
DOC_MAPK_MEF2A_6	9	16	PF00069	0.709
DOC_PP2B_LxvP_1	178	181	PF13499	0.692
DOC_PP4_FxxP_1	283	286	PF00568	0.503
DOC_USP7_MATH_1	114	118	PF00917	0.715
DOC_USP7_MATH_1	155	159	PF00917	0.703
DOC_USP7_MATH_1	336	340	PF00917	0.441
DOC_USP7_MATH_1	52	56	PF00917	0.733
DOC_USP7_MATH_1	67	71	PF00917	0.721
DOC_USP7_MATH_1	84	88	PF00917	0.750
DOC_USP7_UBL2_3	9	13	PF12436	0.702
DOC_WW_Pin1_4	214	219	PF00397	0.696
DOC_WW_Pin1_4	357	362	PF00397	0.357
DOC_WW_Pin1_4	68	73	PF00397	0.801
LIG_14-3-3_CanoR_1	197	207	PF00244	0.678
LIG_FHA_1	222	228	PF00498	0.608
LIG_FHA_1	29	35	PF00498	0.698
LIG_FHA_1	358	364	PF00498	0.357
LIG_FHA_2	215	221	PF00498	0.611
LIG_LIR_Apic_2	281	286	PF02991	0.492
LIG_LIR_Nem_3	176	182	PF02991	0.689
LIG_LIR_Nem_3	281	285	PF02991	0.448
LIG_SH2_CRK	179	183	PF00017	0.660
LIG_SH2_NCK_1	317	321	PF00017	0.391
LIG_SH2_SRC	245	248	PF00017	0.587
LIG_SH2_SRC	317	320	PF00017	0.438
LIG_SH2_STAP1	238	242	PF00017	0.523
LIG_SH2_STAP1	245	249	PF00017	0.541
LIG_SH2_STAP1	310	314	PF00017	0.357
LIG_SH2_STAT5	46	49	PF00017	0.712
LIG_SH3_3	149	155	PF00018	0.687
LIG_SH3_3	335	341	PF00018	0.492
LIG_SH3_3	358	364	PF00018	0.376
LIG_SUMO_SIM_anti_2	33	38	PF11976	0.660
LIG_TRAF2_1	133	136	PF00917	0.656
LIG_TRAF2_1	193	196	PF00917	0.674
LIG_TRFH_1	283	287	PF08558	0.492
LIG_WW_2	152	155	PF00397	0.687
MOD_CK1_1	187	193	PF00069	0.607
MOD_CK1_1	208	214	PF00069	0.643
MOD_CK1_1	255	261	PF00069	0.729
MOD_CK1_1	339	345	PF00069	0.410
MOD_CK2_1	343	349	PF00069	0.438
MOD_CK2_1	50	56	PF00069	0.710
MOD_GlcNHglycan	128	131	PF01048	0.735
MOD_GlcNHglycan	165	168	PF01048	0.758
MOD_GlcNHglycan	175	178	PF01048	0.713
MOD_GlcNHglycan	200	203	PF01048	0.691
MOD_GlcNHglycan	207	210	PF01048	0.680
MOD_GlcNHglycan	213	216	PF01048	0.723
MOD_GlcNHglycan	257	260	PF01048	0.670
MOD_GlcNHglycan	297	300	PF01048	0.332
MOD_GlcNHglycan	334	337	PF01048	0.482
MOD_GlcNHglycan	60	63	PF01048	0.724
MOD_GSK3_1	122	129	PF00069	0.669
MOD_GSK3_1	207	214	PF00069	0.709
MOD_GSK3_1	28	35	PF00069	0.659
MOD_GSK3_1	332	339	PF00069	0.424
MOD_GSK3_1	52	59	PF00069	0.699
MOD_LATS_1	120	126	PF00433	0.529
MOD_N-GLC_1	101	106	PF02516	0.723
MOD_N-GLC_1	126	131	PF02516	0.709
MOD_NEK2_1	173	178	PF00069	0.626
MOD_NEK2_1	184	189	PF00069	0.633
MOD_NEK2_1	252	257	PF00069	0.674
MOD_NEK2_2	245	250	PF00069	0.574
MOD_PIKK_1	142	148	PF00454	0.697
MOD_PIKK_1	208	214	PF00454	0.546
MOD_PIKK_1	25	31	PF00454	0.785
MOD_PIKK_1	329	335	PF00454	0.397
MOD_Plk_1	142	148	PF00069	0.722
MOD_Plk_1	184	190	PF00069	0.609
MOD_Plk_1	245	251	PF00069	0.653
MOD_Plk_1	32	38	PF00069	0.580
MOD_Plk_4	114	120	PF00069	0.783
MOD_Plk_4	32	38	PF00069	0.580
MOD_ProDKin_1	214	220	PF00069	0.694
MOD_ProDKin_1	357	363	PF00069	0.357
MOD_ProDKin_1	68	74	PF00069	0.804
TRG_DiLeu_BaEn_4	195	201	PF01217	0.630
TRG_ENDOCYTIC_2	179	182	PF00928	0.685
TRG_ENDOCYTIC_2	309	312	PF00928	0.331
TRG_NES_CRM1_1	304	318	PF08389	0.319
TRG_NES_CRM1_1	38	49	PF08389	0.636

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PBD4	Leptomonas seymouri	58%	93%
A0A3S7XA21	Leishmania donovani	92%	100%
A4HN89	Leishmania braziliensis	76%	100%
A4IBV7	Leishmania infantum	92%	100%
C9ZYQ2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	33%	100%
E9B6U7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
V5BXW4	Trypanosoma cruzi	34%	100%