Polyadenylate-binding protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

polyadenylate-binding protein 2

Species:

Leishmania major

UniProt:

E9AFP0_LEIMA

TriTrypDb:

LmjF.35.4130 , LMJLV39_350049300 , LMJSD75_350048700

Length:

594

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 6
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 20

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 14

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0000932	P-body	5	2
GO:0005634	nucleus	5	2
GO:0005737	cytoplasm	2	12
GO:0005829	cytosol	2	2
GO:0010494	cytoplasmic stress granule	5	2
GO:0032991	protein-containing complex	1	2
GO:0035770	ribonucleoprotein granule	3	2
GO:0036464	cytoplasmic ribonucleoprotein granule	4	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043228	non-membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0043232	intracellular non-membrane-bounded organelle	4	2
GO:0097165	nuclear stress granule	4	2
GO:0099080	supramolecular complex	2	2
GO:0110165	cellular anatomical entity	1	12
GO:1990904	ribonucleoprotein complex	2	2

Expansion

Sequence features

E9AFP0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFP0

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006396	RNA processing	6	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0009987	cellular process	1	2
GO:0016070	RNA metabolic process	5	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0043170	macromolecule metabolic process	3	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0046483	heterocycle metabolic process	3	2
GO:0071704	organic substance metabolic process	2	2
GO:0090304	nucleic acid metabolic process	4	2
GO:1901360	organic cyclic compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003727	single-stranded RNA binding	5	2
GO:0003729	mRNA binding	5	2
GO:0003730	mRNA 3'-UTR binding	6	2
GO:0005488	binding	1	12
GO:0005515	protein binding	2	2
GO:0008143	poly(A) binding	7	2
GO:0008187	poly-pyrimidine tract binding	6	2
GO:0008266	poly(U) RNA binding	7	2
GO:0031369	translation initiation factor binding	3	2
GO:0031370	eukaryotic initiation factor 4G binding	4	2
GO:0070717	poly-purine tract binding	6	2
GO:0097159	organic cyclic compound binding	2	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	147	151	PF00656	0.548
CLV_C14_Caspase3-7	575	579	PF00656	0.551
CLV_NRD_NRD_1	277	279	PF00675	0.541
CLV_NRD_NRD_1	366	368	PF00675	0.509
CLV_NRD_NRD_1	371	373	PF00675	0.509
CLV_NRD_NRD_1	90	92	PF00675	0.498
CLV_PCSK_FUR_1	275	279	PF00082	0.532
CLV_PCSK_KEX2_1	277	279	PF00082	0.539
CLV_PCSK_KEX2_1	371	373	PF00082	0.517
CLV_PCSK_KEX2_1	89	91	PF00082	0.500
CLV_PCSK_PC1ET2_1	89	91	PF00082	0.515
CLV_PCSK_PC7_1	273	279	PF00082	0.535
CLV_PCSK_PC7_1	367	373	PF00082	0.524
CLV_PCSK_PC7_1	85	91	PF00082	0.511
CLV_PCSK_SKI1_1	112	116	PF00082	0.262
CLV_PCSK_SKI1_1	180	184	PF00082	0.479
CLV_PCSK_SKI1_1	200	204	PF00082	0.310
CLV_PCSK_SKI1_1	358	362	PF00082	0.414
CLV_PCSK_SKI1_1	533	537	PF00082	0.262
CLV_PCSK_SKI1_1	79	83	PF00082	0.428
DOC_ANK_TNKS_1	119	126	PF00023	0.473
DOC_MAPK_gen_1	117	126	PF00069	0.462
DOC_MAPK_HePTP_8	307	319	PF00069	0.487
DOC_MAPK_MEF2A_6	310	319	PF00069	0.473
DOC_MAPK_MEF2A_6	554	562	PF00069	0.462
DOC_PP1_RVXF_1	137	143	PF00149	0.527
DOC_PP2B_PxIxI_1	7	13	PF00149	0.321
DOC_PP4_FxxP_1	3	6	PF00568	0.662
DOC_PP4_FxxP_1	354	357	PF00568	0.540
DOC_PP4_FxxP_1	402	405	PF00568	0.590
DOC_PP4_FxxP_1	415	418	PF00568	0.582
DOC_PP4_FxxP_1	56	59	PF00568	0.462
DOC_USP7_MATH_1	219	223	PF00917	0.578
DOC_USP7_MATH_1	33	37	PF00917	0.462
DOC_USP7_MATH_1	501	505	PF00917	0.708
DOC_USP7_MATH_1	6	10	PF00917	0.446
DOC_USP7_UBL2_3	100	104	PF12436	0.558
DOC_WW_Pin1_4	253	258	PF00397	0.468
DOC_WW_Pin1_4	353	358	PF00397	0.651
DOC_WW_Pin1_4	570	575	PF00397	0.505
LIG_14-3-3_CanoR_1	120	126	PF00244	0.475
LIG_14-3-3_CanoR_1	139	143	PF00244	0.473
LIG_14-3-3_CanoR_1	539	544	PF00244	0.558
LIG_14-3-3_CterR_2	590	594	PF00244	0.624
LIG_BIR_II_1	1	5	PF00653	0.570
LIG_BRCT_BRCA1_1	327	331	PF00533	0.462
LIG_BRCT_BRCA1_1	378	382	PF00533	0.679
LIG_Clathr_ClatBox_1	559	563	PF01394	0.462
LIG_EVH1_2	398	402	PF00568	0.699
LIG_FHA_1	106	112	PF00498	0.473
LIG_FHA_1	312	318	PF00498	0.467
LIG_FHA_1	361	367	PF00498	0.455
LIG_FHA_1	554	560	PF00498	0.471
LIG_FHA_2	573	579	PF00498	0.500
LIG_GBD_Chelix_1	551	559	PF00786	0.358
LIG_LIR_Apic_2	352	357	PF02991	0.625
LIG_LIR_Gen_1	113	123	PF02991	0.527
LIG_LIR_Gen_1	134	142	PF02991	0.484
LIG_LIR_Gen_1	26	34	PF02991	0.473
LIG_LIR_Gen_1	538	549	PF02991	0.462
LIG_LIR_Gen_1	75	83	PF02991	0.626
LIG_LIR_Nem_3	113	118	PF02991	0.472
LIG_LIR_Nem_3	134	140	PF02991	0.475
LIG_LIR_Nem_3	26	31	PF02991	0.473
LIG_LIR_Nem_3	285	290	PF02991	0.448
LIG_LIR_Nem_3	305	311	PF02991	0.353
LIG_LIR_Nem_3	328	334	PF02991	0.471
LIG_LIR_Nem_3	48	54	PF02991	0.471
LIG_LIR_Nem_3	538	544	PF02991	0.462
LIG_LIR_Nem_3	75	80	PF02991	0.542
LIG_LYPXL_yS_3	287	290	PF13949	0.417
LIG_REV1ctd_RIR_1	200	209	PF16727	0.527
LIG_SH2_CRK	137	141	PF00017	0.502
LIG_SH2_CRK	51	55	PF00017	0.474
LIG_SH2_CRK	541	545	PF00017	0.487
LIG_SH2_GRB2like	53	56	PF00017	0.462
LIG_SH2_NCK_1	137	141	PF00017	0.473
LIG_SH2_STAP1	25	29	PF00017	0.462
LIG_SH2_STAP1	541	545	PF00017	0.558
LIG_SH2_STAT3	170	173	PF00017	0.603
LIG_SH2_STAT5	186	189	PF00017	0.491
LIG_SH2_STAT5	228	231	PF00017	0.462
LIG_SH2_STAT5	27	30	PF00017	0.563
LIG_SH2_STAT5	291	294	PF00017	0.462
LIG_SH2_STAT5	53	56	PF00017	0.462
LIG_SH2_STAT5	543	546	PF00017	0.499
LIG_SH2_STAT5	77	80	PF00017	0.619
LIG_SH3_1	494	500	PF00018	0.693
LIG_SH3_3	408	414	PF00018	0.655
LIG_SH3_3	415	421	PF00018	0.624
LIG_SH3_3	479	485	PF00018	0.617
LIG_SH3_3	494	500	PF00018	0.675
LIG_SH3_4	499	506	PF00018	0.780
LIG_SUMO_SIM_anti_2	316	322	PF11976	0.491
LIG_SUMO_SIM_par_1	256	261	PF11976	0.550
LIG_SUMO_SIM_par_1	313	318	PF11976	0.487
LIG_TRAF2_1	240	243	PF00917	0.465
LIG_TRAF2_1	265	268	PF00917	0.570
LIG_TRAF2_1	345	348	PF00917	0.473
LIG_TYR_ITIM	49	54	PF00017	0.462
MOD_CDK_SPK_2	353	358	PF00069	0.651
MOD_CK1_1	105	111	PF00069	0.488
MOD_CK1_1	547	553	PF00069	0.546
MOD_CK2_1	262	268	PF00069	0.508
MOD_GlcNHglycan	145	149	PF01048	0.262
MOD_GlcNHglycan	210	213	PF01048	0.288
MOD_GlcNHglycan	42	45	PF01048	0.358
MOD_GlcNHglycan	91	94	PF01048	0.475
MOD_GSK3_1	258	265	PF00069	0.590
MOD_GSK3_1	311	318	PF00069	0.502
MOD_GSK3_1	349	356	PF00069	0.548
MOD_GSK3_1	486	493	PF00069	0.773
MOD_GSK3_1	572	579	PF00069	0.557
MOD_GSK3_1	68	75	PF00069	0.521
MOD_GSK3_1	85	92	PF00069	0.430
MOD_N-GLC_1	349	354	PF02516	0.358
MOD_NEK2_1	1	6	PF00069	0.676
MOD_NEK2_1	258	263	PF00069	0.444
MOD_NEK2_1	295	300	PF00069	0.469
MOD_NEK2_1	349	354	PF00069	0.467
MOD_NEK2_1	360	365	PF00069	0.409
MOD_NEK2_1	576	581	PF00069	0.532
MOD_NEK2_2	33	38	PF00069	0.462
MOD_NEK2_2	332	337	PF00069	0.462
MOD_PIKK_1	340	346	PF00454	0.546
MOD_PIKK_1	42	48	PF00454	0.473
MOD_PIKK_1	486	492	PF00454	0.764
MOD_PKA_1	89	95	PF00069	0.489
MOD_PKA_2	119	125	PF00069	0.475
MOD_PKA_2	138	144	PF00069	0.473
MOD_PKA_2	89	95	PF00069	0.473
MOD_Plk_1	144	150	PF00069	0.467
MOD_Plk_1	258	264	PF00069	0.563
MOD_Plk_1	295	301	PF00069	0.473
MOD_Plk_2-3	572	578	PF00069	0.556
MOD_Plk_4	315	321	PF00069	0.490
MOD_Plk_4	350	356	PF00069	0.487
MOD_Plk_4	501	507	PF00069	0.709
MOD_Plk_4	539	545	PF00069	0.546
MOD_Plk_4	572	578	PF00069	0.556
MOD_Plk_4	6	12	PF00069	0.420
MOD_ProDKin_1	253	259	PF00069	0.463
MOD_ProDKin_1	353	359	PF00069	0.651
MOD_ProDKin_1	570	576	PF00069	0.505
MOD_SUMO_rev_2	16	24	PF00179	0.456
MOD_SUMO_rev_2	191	199	PF00179	0.487
MOD_SUMO_rev_2	572	582	PF00179	0.462
TRG_ENDOCYTIC_2	137	140	PF00928	0.502
TRG_ENDOCYTIC_2	206	209	PF00928	0.546
TRG_ENDOCYTIC_2	228	231	PF00928	0.527
TRG_ENDOCYTIC_2	25	28	PF00928	0.462
TRG_ENDOCYTIC_2	287	290	PF00928	0.417
TRG_ENDOCYTIC_2	51	54	PF00928	0.474
TRG_ENDOCYTIC_2	541	544	PF00928	0.462
TRG_ENDOCYTIC_2	77	80	PF00928	0.654
TRG_ER_diArg_1	263	266	PF00400	0.510
TRG_ER_diArg_1	275	278	PF00400	0.558
TRG_ER_diArg_1	382	385	PF00400	0.578
TRG_ER_diArg_1	491	494	PF00400	0.655
TRG_NES_CRM1_1	176	191	PF08389	0.496
TRG_NLS_MonoExtN_4	87	93	PF00514	0.501

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P5A9	Leptomonas seymouri	90%	100%
A0A0S4IKZ4	Bodo saltans	57%	100%
A0A0S4JPT3	Bodo saltans	38%	100%
A0A1X0P4T3	Trypanosomatidae	37%	100%
A0A1X0P525	Trypanosomatidae	66%	100%
A0A3Q8IF40	Leishmania donovani	37%	100%
A0A3Q8IGR8	Leishmania donovani	36%	100%
A0A3Q8IMW2	Leishmania donovani	99%	100%
A0A3R7NAH9	Trypanosoma rangeli	36%	100%
A0A422NK82	Trypanosoma rangeli	66%	100%
A1CRM1	Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107)	32%	79%
A1D4K4	Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181)	33%	79%
A2Q848	Aspergillus niger (strain CBS 513.88 / FGSC A1513)	33%	81%
A3LXL0	Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545)	34%	94%
A4HDV5	Leishmania braziliensis	37%	100%
A4HN85	Leishmania braziliensis	91%	100%
A4HNG6	Leishmania braziliensis	37%	100%
A4I150	Leishmania infantum	36%	100%
A4IBV3	Leishmania infantum	99%	100%
A4IC57	Leishmania infantum	36%	100%
A4QUF0	Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)	35%	78%
A5DM21	Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324)	34%	94%
A5DW14	Lodderomyces elongisporus (strain ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239)	36%	90%
C9ZYQ8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	66%	100%
E9AFX7	Leishmania major	36%	100%
E9AX84	Leishmania mexicana (strain MHOM/GT/2001/U1103)	37%	100%
E9B6U3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	98%	100%
E9B730	Leishmania mexicana (strain MHOM/GT/2001/U1103)	36%	100%
F1QB54	Danio rerio	40%	94%
O22173	Arabidopsis thaliana	37%	90%
O64380	Arabidopsis thaliana	39%	90%
P04147	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	39%	100%
P0CP46	Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565)	36%	88%
P0CP47	Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)	36%	88%
P11940	Homo sapiens	40%	93%
P20965	Xenopus laevis	40%	94%
P29341	Mus musculus	40%	93%
P31209	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	35%	91%
P42731	Arabidopsis thaliana	38%	94%
P61286	Bos taurus	40%	93%
Q05196	Arabidopsis thaliana	36%	87%
Q0CR95	Aspergillus terreus (strain NIH 2624 / FGSC A1156)	32%	79%
Q0U1G2	Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173)	32%	80%
Q13310	Homo sapiens	40%	92%
Q1DXH0	Coccidioides immitis (strain RS)	34%	77%
Q1ZXC2	Dictyostelium discoideum	30%	73%
Q2GSX8	Chaetomium globosum (strain ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970)	34%	76%
Q2UK72	Aspergillus oryzae (strain ATCC 42149 / RIB 40)	33%	78%
Q4QA88	Leishmania major	36%	100%
Q4VXU2	Homo sapiens	38%	97%
Q4WK03	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)	33%	79%
Q54BM2	Dictyostelium discoideum	32%	100%
Q5AI15	Candida albicans (strain SC5314 / ATCC MYA-2876)	36%	94%
Q5B630	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	35%	81%
Q5R8F7	Pongo abelii	40%	93%
Q6BI95	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	34%	95%
Q6CDH3	Yarrowia lipolytica (strain CLIB 122 / E 150)	32%	94%
Q6CSV3	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	33%	100%
Q6DEY7	Xenopus tropicalis	38%	94%
Q6FKG4	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	32%	100%
Q6GR16	Xenopus laevis	38%	94%
Q6IP09	Xenopus laevis	39%	94%
Q74ZS6	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	38%	100%
Q7S6N6	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	34%	78%
Q8SR30	Encephalitozoon cuniculi (strain GB-M1)	25%	100%
Q98SP8	Xenopus laevis	38%	94%
Q9EPH8	Rattus norvegicus	40%	93%
Q9FXA2	Arabidopsis thaliana	37%	89%
Q9ZQA8	Arabidopsis thaliana	31%	98%
V5BYR2	Trypanosoma cruzi	36%	100%
V5DUJ1	Trypanosoma cruzi	66%	100%