Putative ATP-dependent RNA helicase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ATP-dependent RNA helicase, putative

Species:

Leishmania major

UniProt:

E9AFM8_LEIMA

TriTrypDb:

LmjF.35.4030 , LMJLV39_350048100 , LMJSD75_350047500

Length:

690

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005730	nucleolus	5	2
GO:0043226	organelle	2	2
GO:0043228	non-membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043232	intracellular non-membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	3
GO:0016020	membrane	2	1

Expansion

Sequence features

E9AFM8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFM8

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	11
GO:0003824	catalytic activity	1	12
GO:0004386	helicase activity	2	12
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0016787	hydrolase activity	2	12
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:0140657	ATP-dependent activity	1	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	304	308	PF00656	0.512
CLV_NRD_NRD_1	202	204	PF00675	0.254
CLV_NRD_NRD_1	330	332	PF00675	0.217
CLV_NRD_NRD_1	427	429	PF00675	0.535
CLV_NRD_NRD_1	438	440	PF00675	0.506
CLV_NRD_NRD_1	453	455	PF00675	0.563
CLV_NRD_NRD_1	456	458	PF00675	0.564
CLV_NRD_NRD_1	477	479	PF00675	0.604
CLV_NRD_NRD_1	495	497	PF00675	0.400
CLV_NRD_NRD_1	652	654	PF00675	0.658
CLV_NRD_NRD_1	679	681	PF00675	0.586
CLV_NRD_NRD_1	686	688	PF00675	0.570
CLV_PCSK_FUR_1	200	204	PF00082	0.189
CLV_PCSK_FUR_1	454	458	PF00082	0.679
CLV_PCSK_FUR_1	579	583	PF00082	0.569
CLV_PCSK_KEX2_1	196	198	PF00082	0.251
CLV_PCSK_KEX2_1	202	204	PF00082	0.251
CLV_PCSK_KEX2_1	320	322	PF00082	0.262
CLV_PCSK_KEX2_1	330	332	PF00082	0.262
CLV_PCSK_KEX2_1	427	429	PF00082	0.503
CLV_PCSK_KEX2_1	438	440	PF00082	0.562
CLV_PCSK_KEX2_1	453	455	PF00082	0.555
CLV_PCSK_KEX2_1	456	458	PF00082	0.530
CLV_PCSK_KEX2_1	477	479	PF00082	0.657
CLV_PCSK_KEX2_1	495	497	PF00082	0.362
CLV_PCSK_KEX2_1	554	556	PF00082	0.584
CLV_PCSK_KEX2_1	581	583	PF00082	0.515
CLV_PCSK_KEX2_1	606	608	PF00082	0.663
CLV_PCSK_KEX2_1	652	654	PF00082	0.603
CLV_PCSK_KEX2_1	98	100	PF00082	0.335
CLV_PCSK_PC1ET2_1	196	198	PF00082	0.262
CLV_PCSK_PC1ET2_1	320	322	PF00082	0.262
CLV_PCSK_PC1ET2_1	438	440	PF00082	0.577
CLV_PCSK_PC1ET2_1	477	479	PF00082	0.663
CLV_PCSK_PC1ET2_1	554	556	PF00082	0.584
CLV_PCSK_PC1ET2_1	581	583	PF00082	0.555
CLV_PCSK_PC1ET2_1	606	608	PF00082	0.663
CLV_PCSK_PC1ET2_1	98	100	PF00082	0.335
CLV_PCSK_PC7_1	326	332	PF00082	0.309
CLV_PCSK_SKI1_1	129	133	PF00082	0.259
CLV_PCSK_SKI1_1	2	6	PF00082	0.469
CLV_PCSK_SKI1_1	326	330	PF00082	0.262
CLV_PCSK_SKI1_1	333	337	PF00082	0.262
CLV_PCSK_SKI1_1	428	432	PF00082	0.582
CLV_PCSK_SKI1_1	474	478	PF00082	0.637
CLV_PCSK_SKI1_1	479	483	PF00082	0.541
CLV_PCSK_SKI1_1	500	504	PF00082	0.359
CLV_PCSK_SKI1_1	525	529	PF00082	0.303
CLV_PCSK_SKI1_1	620	624	PF00082	0.603
CLV_PCSK_SKI1_1	98	102	PF00082	0.262
DEG_APCC_DBOX_1	187	195	PF00400	0.537
DEG_Nend_UBRbox_1	1	4	PF02207	0.550
DOC_CKS1_1	147	152	PF01111	0.451
DOC_CYCLIN_RxL_1	422	434	PF00134	0.607
DOC_CYCLIN_RxL_1	96	105	PF00134	0.492
DOC_MAPK_DCC_7	344	353	PF00069	0.462
DOC_MAPK_gen_1	276	284	PF00069	0.442
DOC_MAPK_gen_1	330	337	PF00069	0.472
DOC_MAPK_gen_1	375	385	PF00069	0.527
DOC_MAPK_gen_1	554	563	PF00069	0.491
DOC_MAPK_gen_1	612	621	PF00069	0.567
DOC_MAPK_HePTP_8	341	353	PF00069	0.462
DOC_MAPK_MEF2A_6	224	233	PF00069	0.426
DOC_MAPK_MEF2A_6	276	284	PF00069	0.437
DOC_MAPK_MEF2A_6	344	353	PF00069	0.462
DOC_MAPK_MEF2A_6	5	13	PF00069	0.450
DOC_MAPK_MEF2A_6	554	563	PF00069	0.434
DOC_PP1_RVXF_1	179	186	PF00149	0.451
DOC_PP1_RVXF_1	265	272	PF00149	0.462
DOC_PP1_RVXF_1	379	386	PF00149	0.443
DOC_PP1_RVXF_1	97	104	PF00149	0.472
DOC_PP2B_LxvP_1	236	239	PF13499	0.476
DOC_PP2B_LxvP_1	346	349	PF13499	0.451
DOC_PP2B_LxvP_1	559	562	PF13499	0.407
DOC_PP2B_LxvP_1	82	85	PF13499	0.556
DOC_PP4_FxxP_1	385	388	PF00568	0.340
DOC_PP4_FxxP_1	545	548	PF00568	0.303
DOC_USP7_MATH_1	403	407	PF00917	0.336
DOC_USP7_MATH_1	511	515	PF00917	0.315
DOC_WW_Pin1_4	109	114	PF00397	0.556
DOC_WW_Pin1_4	146	151	PF00397	0.451
DOC_WW_Pin1_4	485	490	PF00397	0.390
LIG_14-3-3_CanoR_1	224	230	PF00244	0.418
LIG_14-3-3_CanoR_1	260	268	PF00244	0.300
LIG_14-3-3_CanoR_1	378	384	PF00244	0.507
LIG_14-3-3_CanoR_1	427	431	PF00244	0.544
LIG_14-3-3_CanoR_1	510	516	PF00244	0.303
LIG_14-3-3_CanoR_1	607	616	PF00244	0.599
LIG_BRCT_BRCA1_1	252	256	PF00533	0.397
LIG_BRCT_BRCA1_1	381	385	PF00533	0.379
LIG_Clathr_ClatBox_1	173	177	PF01394	0.462
LIG_FHA_1	210	216	PF00498	0.462
LIG_FHA_1	228	234	PF00498	0.491
LIG_FHA_1	28	34	PF00498	0.451
LIG_FHA_1	616	622	PF00498	0.440
LIG_FHA_2	34	40	PF00498	0.537
LIG_LIR_Apic_2	382	388	PF02991	0.345
LIG_LIR_Apic_2	51	57	PF02991	0.451
LIG_LIR_Gen_1	159	168	PF02991	0.434
LIG_LIR_Gen_1	169	179	PF02991	0.452
LIG_LIR_Gen_1	291	302	PF02991	0.492
LIG_LIR_Gen_1	36	45	PF02991	0.537
LIG_LIR_Gen_1	390	399	PF02991	0.391
LIG_LIR_Gen_1	539	548	PF02991	0.297
LIG_LIR_Gen_1	644	651	PF02991	0.645
LIG_LIR_Nem_3	121	125	PF02991	0.516
LIG_LIR_Nem_3	159	165	PF02991	0.434
LIG_LIR_Nem_3	169	174	PF02991	0.452
LIG_LIR_Nem_3	253	258	PF02991	0.475
LIG_LIR_Nem_3	291	297	PF02991	0.492
LIG_LIR_Nem_3	36	41	PF02991	0.537
LIG_LIR_Nem_3	390	396	PF02991	0.360
LIG_LIR_Nem_3	539	545	PF02991	0.305
LIG_LIR_Nem_3	565	570	PF02991	0.492
LIG_MLH1_MIPbox_1	252	256	PF16413	0.397
LIG_NRBOX	152	158	PF00104	0.462
LIG_NRBOX	190	196	PF00104	0.528
LIG_NRBOX	58	64	PF00104	0.512
LIG_NRBOX	637	643	PF00104	0.639
LIG_NRP_CendR_1	687	690	PF00754	0.629
LIG_PCNA_yPIPBox_3	629	642	PF02747	0.535
LIG_PTB_Apo_2	73	80	PF02174	0.528
LIG_Rb_LxCxE_1	51	73	PF01857	0.476
LIG_SH2_CRK	162	166	PF00017	0.435
LIG_SH2_CRK	357	361	PF00017	0.462
LIG_SH2_CRK	38	42	PF00017	0.516
LIG_SH2_GRB2like	162	165	PF00017	0.417
LIG_SH2_GRB2like	393	396	PF00017	0.470
LIG_SH2_SRC	357	360	PF00017	0.451
LIG_SH2_SRC	54	57	PF00017	0.511
LIG_SH2_STAP1	154	158	PF00017	0.451
LIG_SH2_STAP1	162	166	PF00017	0.451
LIG_SH2_STAP1	38	42	PF00017	0.474
LIG_SH2_STAT3	120	123	PF00017	0.451
LIG_SH2_STAT3	526	529	PF00017	0.319
LIG_SH2_STAT5	125	128	PF00017	0.431
LIG_SH2_STAT5	283	286	PF00017	0.448
LIG_SH2_STAT5	393	396	PF00017	0.362
LIG_SH2_STAT5	526	529	PF00017	0.319
LIG_SH2_STAT5	54	57	PF00017	0.450
LIG_SH2_STAT5	647	650	PF00017	0.493
LIG_SH3_1	357	363	PF00018	0.462
LIG_SH3_3	144	150	PF00018	0.512
LIG_SH3_3	243	249	PF00018	0.495
LIG_SH3_3	357	363	PF00018	0.462
LIG_SUMO_SIM_anti_2	172	178	PF11976	0.462
LIG_SUMO_SIM_par_1	172	178	PF11976	0.462
LIG_SUMO_SIM_par_1	225	230	PF11976	0.451
LIG_SUMO_SIM_par_1	531	537	PF11976	0.319
LIG_SUMO_SIM_par_1	80	86	PF11976	0.473
LIG_TRAF2_1	585	588	PF00917	0.603
LIG_TRAF2_2	403	408	PF00917	0.486
LIG_TYR_ITSM	34	41	PF00017	0.424
LIG_UBA3_1	173	181	PF00899	0.338
LIG_UBA3_1	621	626	PF00899	0.675
LIG_WRC_WIRS_1	512	517	PF05994	0.303
MOD_CDK_SPxK_1	146	152	PF00069	0.303
MOD_CK1_1	379	385	PF00069	0.499
MOD_CK1_1	48	54	PF00069	0.296
MOD_CK1_1	627	633	PF00069	0.660
MOD_CK1_1	673	679	PF00069	0.628
MOD_CK1_1	7	13	PF00069	0.436
MOD_CK2_1	166	172	PF00069	0.338
MOD_CK2_1	449	455	PF00069	0.609
MOD_CK2_1	622	628	PF00069	0.521
MOD_CK2_1	673	679	PF00069	0.522
MOD_GlcNHglycan	207	210	PF01048	0.303
MOD_GlcNHglycan	297	300	PF01048	0.373
MOD_GlcNHglycan	378	381	PF01048	0.551
MOD_GlcNHglycan	47	50	PF01048	0.293
MOD_GlcNHglycan	587	592	PF01048	0.581
MOD_GlcNHglycan	675	678	PF01048	0.608
MOD_GSK3_1	201	208	PF00069	0.303
MOD_GSK3_1	426	433	PF00069	0.497
MOD_N-GLC_1	109	114	PF02516	0.410
MOD_NEK2_1	156	161	PF00069	0.303
MOD_NEK2_1	166	171	PF00069	0.303
MOD_NEK2_1	18	23	PF00069	0.495
MOD_NEK2_1	207	212	PF00069	0.303
MOD_NEK2_1	256	261	PF00069	0.506
MOD_NEK2_1	271	276	PF00069	0.290
MOD_NEK2_1	33	38	PF00069	0.388
MOD_NEK2_1	376	381	PF00069	0.520
MOD_NEK2_1	622	627	PF00069	0.592
MOD_NEK2_1	642	647	PF00069	0.322
MOD_PIKK_1	166	172	PF00454	0.450
MOD_PIKK_1	207	213	PF00454	0.303
MOD_PIKK_1	469	475	PF00454	0.658
MOD_PKA_2	201	207	PF00069	0.303
MOD_PKA_2	259	265	PF00069	0.353
MOD_PKA_2	329	335	PF00069	0.256
MOD_PKA_2	426	432	PF00069	0.480
MOD_Plk_1	587	593	PF00069	0.631
MOD_Plk_1	670	676	PF00069	0.634
MOD_Plk_2-3	410	416	PF00069	0.534
MOD_Plk_2-3	596	602	PF00069	0.506
MOD_Plk_4	305	311	PF00069	0.355
MOD_Plk_4	362	368	PF00069	0.303
MOD_Plk_4	379	385	PF00069	0.378
MOD_Plk_4	487	493	PF00069	0.395
MOD_Plk_4	511	517	PF00069	0.303
MOD_Plk_4	642	648	PF00069	0.518
MOD_Plk_4	670	676	PF00069	0.616
MOD_Plk_4	9	15	PF00069	0.300
MOD_ProDKin_1	109	115	PF00069	0.450
MOD_ProDKin_1	146	152	PF00069	0.303
MOD_ProDKin_1	485	491	PF00069	0.383
MOD_SUMO_for_1	302	305	PF00179	0.217
MOD_SUMO_for_1	316	319	PF00179	0.319
MOD_SUMO_for_1	648	651	PF00179	0.498
MOD_SUMO_rev_2	673	683	PF00179	0.555
MOD_SUMO_rev_2	69	77	PF00179	0.323
TRG_DiLeu_BaEn_1	178	183	PF01217	0.303
TRG_DiLeu_BaEn_1	617	622	PF01217	0.647
TRG_DiLeu_BaEn_1	637	642	PF01217	0.336
TRG_DiLeu_BaEn_2	391	397	PF01217	0.403
TRG_DiLeu_BaEn_2	565	571	PF01217	0.512
TRG_ENDOCYTIC_2	154	157	PF00928	0.303
TRG_ENDOCYTIC_2	162	165	PF00928	0.303
TRG_ENDOCYTIC_2	294	297	PF00928	0.347
TRG_ENDOCYTIC_2	38	41	PF00928	0.355
TRG_ENDOCYTIC_2	393	396	PF00928	0.362
TRG_ENDOCYTIC_2	647	650	PF00928	0.639
TRG_ER_diArg_1	199	202	PF00400	0.368
TRG_ER_diArg_1	330	333	PF00400	0.294
TRG_ER_diArg_1	494	496	PF00400	0.424
TRG_ER_diArg_1	555	558	PF00400	0.612
TRG_ER_diArg_1	63	66	PF00400	0.424
TRG_ER_FFAT_2	668	679	PF00635	0.579
TRG_NES_CRM1_1	291	305	PF08389	0.221
TRG_NES_CRM1_1	390	400	PF08389	0.393
TRG_NES_CRM1_1	552	565	PF08389	0.424
TRG_NES_CRM1_1	614	628	PF08389	0.671
TRG_NLS_MonoExtC_3	605	611	PF00514	0.603
TRG_NLS_MonoExtN_4	603	610	PF00514	0.649
TRG_Pf-PMV_PEXEL_1	213	217	PF00026	0.317
TRG_Pf-PMV_PEXEL_1	525	529	PF00026	0.303
TRG_Pf-PMV_PEXEL_1	87	91	PF00026	0.303

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P4X4	Leptomonas seymouri	81%	99%
A0A0S4IX79	Bodo saltans	57%	95%
A0A0S4JD47	Bodo saltans	29%	84%
A0A1X0NVK0	Trypanosomatidae	31%	96%
A0A1X0P5E1	Trypanosomatidae	65%	97%
A0A1X0P9U0	Trypanosomatidae	28%	83%
A0A3Q8ID91	Leishmania donovani	31%	100%
A0A3R7M1K3	Trypanosoma rangeli	30%	96%
A0A3R7NR45	Trypanosoma rangeli	29%	84%
A0A3S5H6T7	Leishmania donovani	27%	100%
A0A3S5IQZ7	Trypanosoma rangeli	29%	87%
A0A3S7X5R1	Leishmania donovani	32%	89%
A0A3S7X9W0	Leishmania donovani	95%	100%
A0A3S7XAT8	Leishmania donovani	35%	100%
A0A422NKE3	Trypanosoma rangeli	64%	95%
A4H7Y3	Leishmania braziliensis	27%	100%
A4HGR1	Leishmania braziliensis	32%	100%
A4HK38	Leishmania braziliensis	27%	100%
A4HN73	Leishmania braziliensis	89%	100%
A4HP49	Leishmania braziliensis	34%	100%
A4HT33	Leishmania infantum	30%	100%
A4HWB0	Leishmania infantum	27%	100%
A4HZF8	Leishmania infantum	26%	100%
A4I3T6	Leishmania infantum	31%	100%
A4I846	Leishmania infantum	32%	89%
A4IBU1	Leishmania infantum	95%	100%
A4IDF6	Leishmania infantum	35%	100%
A5DAR2	Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324)	27%	92%
A6R918	Ajellomyces capsulatus (strain NAm1 / WU24)	35%	100%
A6RMZ2	Botryotinia fuckeliana (strain B05.10)	35%	100%
A6ZZY8	Saccharomyces cerevisiae (strain YJM789)	26%	93%
A7F2S3	Sclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1)	35%	100%
A7TJS7	Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294 / BCRC 21397 / CBS 2163 / NBRC 10782 / NRRL Y-8283 / UCD 57-17)	34%	100%
A7TNT1	Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294 / BCRC 21397 / CBS 2163 / NBRC 10782 / NRRL Y-8283 / UCD 57-17)	25%	94%
C9ZYS3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	68%	89%
D0AAB3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	31%	95%
E9AJG4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	100%
E9ASV7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	100%
E9B028	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
E9B6T1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	100%
P0CR08	Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565)	32%	92%
P0CR09	Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)	32%	92%
P36120	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	26%	93%
Q1EB38	Coccidioides immitis (strain RS)	31%	74%
Q4Q1N9	Leishmania major	35%	100%
Q4Q858	Leishmania major	31%	100%
Q59S50	Candida albicans (strain SC5314 / ATCC MYA-2876)	27%	95%
Q6CT85	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	31%	100%
Q6NZQ2	Mus musculus	29%	100%
Q9H8H2	Homo sapiens	28%	81%
V5DUK0	Trypanosoma cruzi	64%	90%