CULLIN_2 domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Cullin family, putative

Species:

Leishmania major

UniProt:

E9AFJ9_LEIMA

TriTrypDb:

LmjF.35.3750 , LMJLV39_350045100 , LMJSD75_350044500

Length:

875

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0000151	ubiquitin ligase complex	3	2
GO:0000152	nuclear ubiquitin ligase complex	3	2
GO:0005680	anaphase-promoting complex	4	2
GO:0005737	cytoplasm	2	2
GO:0031461	cullin-RING ubiquitin ligase complex	4	2
GO:0032991	protein-containing complex	1	2
GO:0051286	cell tip	3	2
GO:0060187	cell pole	2	2
GO:0110165	cellular anatomical entity	1	2
GO:0140513	nuclear protein-containing complex	2	2
GO:0140535	intracellular protein-containing complex	2	2
GO:1902494	catalytic complex	2	2
GO:1990234	transferase complex	3	2

Expansion

Sequence features

E9AFJ9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFJ9

Function

Biological processes
Term	Name	Level	Count
GO:0000209	protein polyubiquitination	8	2
GO:0006508	proteolysis	4	10
GO:0006511	ubiquitin-dependent protein catabolic process	7	10
GO:0006807	nitrogen compound metabolic process	2	10
GO:0007091	metaphase/anaphase transition of mitotic cell cycle	5	2
GO:0008152	metabolic process	1	10
GO:0009056	catabolic process	2	10
GO:0009057	macromolecule catabolic process	4	10
GO:0009987	cellular process	1	10
GO:0010564	regulation of cell cycle process	5	2
GO:0010965	regulation of mitotic sister chromatid separation	6	2
GO:0016567	protein ubiquitination	7	2
GO:0019538	protein metabolic process	3	10
GO:0019941	modification-dependent protein catabolic process	6	10
GO:0022402	cell cycle process	2	2
GO:0032446	protein modification by small protein conjugation	6	2
GO:0033043	regulation of organelle organization	5	2
GO:0033044	regulation of chromosome organization	6	2
GO:0033045	regulation of sister chromatid segregation	5	2
GO:0036211	protein modification process	4	2
GO:0043170	macromolecule metabolic process	3	10
GO:0043412	macromolecule modification	4	2
GO:0043632	modification-dependent macromolecule catabolic process	5	10
GO:0044237	cellular metabolic process	2	10
GO:0044238	primary metabolic process	2	10
GO:0044248	cellular catabolic process	3	10
GO:0044260	obsolete cellular macromolecule metabolic process	3	10
GO:0044265	obsolete cellular macromolecule catabolic process	4	10
GO:0044770	cell cycle phase transition	3	2
GO:0044772	mitotic cell cycle phase transition	4	2
GO:0044784	metaphase/anaphase transition of cell cycle	4	2
GO:0050789	regulation of biological process	2	2
GO:0050794	regulation of cellular process	3	2
GO:0051128	regulation of cellular component organization	4	2
GO:0051603	proteolysis involved in protein catabolic process	5	10
GO:0051726	regulation of cell cycle	4	2
GO:0051983	regulation of chromosome segregation	4	2
GO:0065007	biological regulation	1	2
GO:0070647	protein modification by small protein conjugation or removal	5	2
GO:0070979	protein K11-linked ubiquitination	9	2
GO:0071704	organic substance metabolic process	2	10
GO:1901564	organonitrogen compound metabolic process	3	10
GO:1901575	organic substance catabolic process	3	10
GO:1903047	mitotic cell cycle process	3	2
GO:1905818	regulation of chromosome separation	5	2
GO:0007049	cell cycle	2	4
GO:0051301	cell division	2	4

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	10
GO:0005515	protein binding	2	10
GO:0019899	enzyme binding	3	10
GO:0031625	ubiquitin protein ligase binding	5	10
GO:0044389	ubiquitin-like protein ligase binding	4	10

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	186	190	PF00656	0.748
CLV_C14_Caspase3-7	19	23	PF00656	0.549
CLV_C14_Caspase3-7	198	202	PF00656	0.748
CLV_C14_Caspase3-7	373	377	PF00656	0.447
CLV_C14_Caspase3-7	665	669	PF00656	0.511
CLV_MEL_PAP_1	70	76	PF00089	0.536
CLV_NRD_NRD_1	175	177	PF00675	0.534
CLV_NRD_NRD_1	306	308	PF00675	0.695
CLV_NRD_NRD_1	362	364	PF00675	0.392
CLV_NRD_NRD_1	459	461	PF00675	0.459
CLV_NRD_NRD_1	531	533	PF00675	0.559
CLV_NRD_NRD_1	589	591	PF00675	0.270
CLV_PCSK_KEX2_1	306	308	PF00082	0.695
CLV_PCSK_KEX2_1	361	363	PF00082	0.393
CLV_PCSK_KEX2_1	458	460	PF00082	0.465
CLV_PCSK_KEX2_1	531	533	PF00082	0.535
CLV_PCSK_KEX2_1	589	591	PF00082	0.270
CLV_PCSK_KEX2_1	675	677	PF00082	0.295
CLV_PCSK_PC1ET2_1	675	677	PF00082	0.295
CLV_PCSK_SKI1_1	140	144	PF00082	0.544
CLV_PCSK_SKI1_1	157	161	PF00082	0.281
CLV_PCSK_SKI1_1	169	173	PF00082	0.398
CLV_PCSK_SKI1_1	225	229	PF00082	0.505
CLV_PCSK_SKI1_1	239	243	PF00082	0.359
CLV_PCSK_SKI1_1	278	282	PF00082	0.470
CLV_PCSK_SKI1_1	352	356	PF00082	0.494
CLV_PCSK_SKI1_1	405	409	PF00082	0.427
CLV_PCSK_SKI1_1	523	527	PF00082	0.428
CLV_PCSK_SKI1_1	531	535	PF00082	0.418
CLV_PCSK_SKI1_1	567	571	PF00082	0.270
CLV_PCSK_SKI1_1	675	679	PF00082	0.295
CLV_PCSK_SKI1_1	815	819	PF00082	0.490
CLV_Separin_Metazoa	851	855	PF03568	0.548
DEG_APCC_DBOX_1	246	254	PF00400	0.582
DEG_APCC_DBOX_1	522	530	PF00400	0.415
DEG_SCF_TRCP1_1	189	195	PF00400	0.561
DEG_SCF_TRCP1_1	647	652	PF00400	0.402
DEG_SPOP_SBC_1	311	315	PF00917	0.773
DEG_SPOP_SBC_1	826	830	PF00917	0.490
DOC_CDC14_PxL_1	683	691	PF14671	0.546
DOC_CKS1_1	126	131	PF01111	0.469
DOC_CKS1_1	389	394	PF01111	0.604
DOC_CKS1_1	734	739	PF01111	0.449
DOC_CYCLIN_RxL_1	402	410	PF00134	0.509
DOC_CYCLIN_RxL_1	518	528	PF00134	0.440
DOC_MAPK_gen_1	516	524	PF00069	0.557
DOC_MAPK_HePTP_8	70	82	PF00069	0.536
DOC_MAPK_MEF2A_6	418	427	PF00069	0.412
DOC_MAPK_MEF2A_6	518	526	PF00069	0.430
DOC_MAPK_MEF2A_6	73	82	PF00069	0.538
DOC_MAPK_MEF2A_6	760	767	PF00069	0.550
DOC_PP1_RVXF_1	262	269	PF00149	0.544
DOC_PP4_FxxP_1	734	737	PF00568	0.388
DOC_USP7_MATH_1	187	191	PF00917	0.764
DOC_USP7_MATH_1	202	206	PF00917	0.517
DOC_USP7_MATH_1	330	334	PF00917	0.648
DOC_USP7_MATH_1	622	626	PF00917	0.531
DOC_USP7_MATH_1	638	642	PF00917	0.506
DOC_USP7_MATH_1	826	830	PF00917	0.563
DOC_USP7_MATH_1	831	835	PF00917	0.530
DOC_USP7_UBL2_3	699	703	PF12436	0.574
DOC_USP7_UBL2_3	837	841	PF12436	0.579
DOC_WW_Pin1_4	125	130	PF00397	0.472
DOC_WW_Pin1_4	210	215	PF00397	0.748
DOC_WW_Pin1_4	388	393	PF00397	0.555
DOC_WW_Pin1_4	42	47	PF00397	0.647
DOC_WW_Pin1_4	554	559	PF00397	0.542
DOC_WW_Pin1_4	618	623	PF00397	0.560
DOC_WW_Pin1_4	733	738	PF00397	0.554
DOC_WW_Pin1_4	759	764	PF00397	0.563
LIG_14-3-3_CanoR_1	113	122	PF00244	0.489
LIG_14-3-3_CanoR_1	249	257	PF00244	0.506
LIG_14-3-3_CanoR_1	682	686	PF00244	0.574
LIG_14-3-3_CanoR_1	73	80	PF00244	0.519
LIG_14-3-3_CanoR_1	731	737	PF00244	0.520
LIG_14-3-3_CanoR_1	815	821	PF00244	0.553
LIG_14-3-3_CanoR_1	824	832	PF00244	0.556
LIG_Actin_WH2_2	127	142	PF00022	0.462
LIG_APCC_ABBA_1	29	34	PF00400	0.566
LIG_BIR_III_2	329	333	PF00653	0.752
LIG_BRCT_BRCA1_1	253	257	PF00533	0.568
LIG_BRCT_BRCA1_1	443	447	PF00533	0.417
LIG_BRCT_BRCA1_1	783	787	PF00533	0.580
LIG_CaM_IQ_9	582	598	PF13499	0.481
LIG_CSL_BTD_1	633	636	PF09270	0.556
LIG_CSL_BTD_1	684	687	PF09270	0.546
LIG_EVH1_1	634	638	PF00568	0.418
LIG_FHA_1	126	132	PF00498	0.421
LIG_FHA_1	144	150	PF00498	0.484
LIG_FHA_1	24	30	PF00498	0.530
LIG_FHA_1	257	263	PF00498	0.543
LIG_FHA_1	396	402	PF00498	0.541
LIG_FHA_1	417	423	PF00498	0.396
LIG_FHA_1	770	776	PF00498	0.448
LIG_FHA_1	816	822	PF00498	0.447
LIG_FHA_2	148	154	PF00498	0.627
LIG_FHA_2	193	199	PF00498	0.764
LIG_FHA_2	311	317	PF00498	0.591
LIG_FHA_2	415	421	PF00498	0.448
LIG_FHA_2	554	560	PF00498	0.470
LIG_FHA_2	571	577	PF00498	0.574
LIG_FHA_2	67	73	PF00498	0.458
LIG_FHA_2	793	799	PF00498	0.683
LIG_GBD_Chelix_1	131	139	PF00786	0.538
LIG_LIR_Gen_1	365	375	PF02991	0.416
LIG_LIR_Gen_1	536	546	PF02991	0.385
LIG_LIR_Gen_1	784	794	PF02991	0.662
LIG_LIR_Nem_3	110	115	PF02991	0.491
LIG_LIR_Nem_3	365	371	PF02991	0.405
LIG_LIR_Nem_3	632	637	PF02991	0.473
LIG_LIR_Nem_3	672	677	PF02991	0.527
LIG_MYND_1	608	612	PF01753	0.511
LIG_MYND_3	102	106	PF01753	0.563
LIG_MYND_3	378	382	PF01753	0.536
LIG_NRBOX	350	356	PF00104	0.407
LIG_OCRL_FandH_1	162	174	PF00620	0.411
LIG_PCNA_yPIPBox_3	841	853	PF02747	0.486
LIG_Pex14_2	161	165	PF04695	0.313
LIG_Pex14_2	433	437	PF04695	0.507
LIG_Pex14_2	717	721	PF04695	0.511
LIG_PTB_Apo_2	493	500	PF02174	0.536
LIG_Rb_pABgroove_1	711	719	PF01858	0.556
LIG_RPA_C_Fungi	584	596	PF08784	0.467
LIG_SH2_CRK	112	116	PF00017	0.539
LIG_SH2_CRK	674	678	PF00017	0.408
LIG_SH2_CRK	870	874	PF00017	0.494
LIG_SH2_NCK_1	870	874	PF00017	0.555
LIG_SH2_SRC	4	7	PF00017	0.617
LIG_SH2_STAT3	386	389	PF00017	0.542
LIG_SH2_STAT5	386	389	PF00017	0.507
LIG_SH3_3	468	474	PF00018	0.607
LIG_SH3_3	477	483	PF00018	0.653
LIG_SH3_3	632	638	PF00018	0.365
LIG_SH3_3	705	711	PF00018	0.380
LIG_SH3_3	97	103	PF00018	0.559
LIG_SH3_4	549	556	PF00018	0.380
LIG_SUMO_SIM_anti_2	419	427	PF11976	0.414
LIG_SUMO_SIM_anti_2	50	59	PF11976	0.487
LIG_SUMO_SIM_anti_2	625	630	PF11976	0.365
LIG_SUMO_SIM_anti_2	742	748	PF11976	0.358
LIG_SUMO_SIM_anti_2	848	855	PF11976	0.460
LIG_SUMO_SIM_par_1	352	358	PF11976	0.408
LIG_SUMO_SIM_par_1	712	718	PF11976	0.401
LIG_SUMO_SIM_par_1	860	866	PF11976	0.554
LIG_SxIP_EBH_1	522	532	PF03271	0.577
LIG_TRAF2_1	150	153	PF00917	0.610
LIG_TRAF2_1	557	560	PF00917	0.338
LIG_TRAF2_1	662	665	PF00917	0.380
LIG_TRAF2_1	795	798	PF00917	0.783
LIG_TRFH_1	870	874	PF08558	0.558
LIG_WRC_WIRS_1	160	165	PF05994	0.507
LIG_WRC_WIRS_1	853	858	PF05994	0.528
LIG_WW_2	635	638	PF00397	0.435
MOD_CK1_1	218	224	PF00069	0.530
MOD_CK1_1	251	257	PF00069	0.515
MOD_CK1_1	294	300	PF00069	0.760
MOD_CK1_1	333	339	PF00069	0.640
MOD_CK1_1	44	50	PF00069	0.605
MOD_CK1_1	448	454	PF00069	0.547
MOD_CK1_1	627	633	PF00069	0.343
MOD_CK2_1	147	153	PF00069	0.584
MOD_CK2_1	192	198	PF00069	0.771
MOD_CK2_1	237	243	PF00069	0.521
MOD_CK2_1	264	270	PF00069	0.545
MOD_CK2_1	281	287	PF00069	0.392
MOD_CK2_1	310	316	PF00069	0.769
MOD_CK2_1	387	393	PF00069	0.561
MOD_CK2_1	4	10	PF00069	0.599
MOD_CK2_1	553	559	PF00069	0.322
MOD_CK2_1	598	604	PF00069	0.310
MOD_CK2_1	66	72	PF00069	0.439
MOD_CK2_1	792	798	PF00069	0.727
MOD_GlcNHglycan	123	126	PF01048	0.519
MOD_GlcNHglycan	189	192	PF01048	0.694
MOD_GlcNHglycan	204	207	PF01048	0.621
MOD_GlcNHglycan	210	213	PF01048	0.609
MOD_GlcNHglycan	230	233	PF01048	0.341
MOD_GlcNHglycan	294	297	PF01048	0.774
MOD_GlcNHglycan	300	305	PF01048	0.667
MOD_GlcNHglycan	34	37	PF01048	0.594
MOD_GlcNHglycan	393	396	PF01048	0.545
MOD_GlcNHglycan	475	478	PF01048	0.467
MOD_GlcNHglycan	50	53	PF01048	0.540
MOD_GlcNHglycan	58	61	PF01048	0.462
MOD_GlcNHglycan	615	618	PF01048	0.470
MOD_GlcNHglycan	641	644	PF01048	0.365
MOD_GlcNHglycan	647	650	PF01048	0.400
MOD_GlcNHglycan	651	654	PF01048	0.337
MOD_GlcNHglycan	74	77	PF01048	0.267
MOD_GlcNHglycan	783	786	PF01048	0.636
MOD_GlcNHglycan	796	801	PF01048	0.754
MOD_GlcNHglycan	86	89	PF01048	0.540
MOD_GlcNHglycan	92	95	PF01048	0.580
MOD_GSK3_1	121	128	PF00069	0.553
MOD_GSK3_1	143	150	PF00069	0.568
MOD_GSK3_1	204	211	PF00069	0.707
MOD_GSK3_1	251	258	PF00069	0.572
MOD_GSK3_1	281	288	PF00069	0.586
MOD_GSK3_1	387	394	PF00069	0.552
MOD_GSK3_1	41	48	PF00069	0.609
MOD_GSK3_1	441	448	PF00069	0.463
MOD_GSK3_1	483	490	PF00069	0.687
MOD_GSK3_1	585	592	PF00069	0.332
MOD_GSK3_1	618	625	PF00069	0.447
MOD_GSK3_1	645	652	PF00069	0.340
MOD_GSK3_1	681	688	PF00069	0.439
MOD_GSK3_1	72	79	PF00069	0.521
MOD_GSK3_1	769	776	PF00069	0.464
MOD_GSK3_1	792	799	PF00069	0.674
MOD_GSK3_1	816	823	PF00069	0.548
MOD_GSK3_1	827	834	PF00069	0.548
MOD_GSK3_1	90	97	PF00069	0.398
MOD_N-GLC_1	202	207	PF02516	0.569
MOD_N-GLC_1	228	233	PF02516	0.576
MOD_NEK2_1	143	148	PF00069	0.410
MOD_NEK2_1	237	242	PF00069	0.474
MOD_NEK2_1	269	274	PF00069	0.544
MOD_NEK2_1	291	296	PF00069	0.750
MOD_NEK2_1	32	37	PF00069	0.581
MOD_NEK2_1	355	360	PF00069	0.368
MOD_NEK2_1	400	405	PF00069	0.421
MOD_NEK2_1	407	412	PF00069	0.416
MOD_NEK2_1	426	431	PF00069	0.362
MOD_NEK2_1	525	530	PF00069	0.479
MOD_NEK2_1	56	61	PF00069	0.507
MOD_NEK2_1	570	575	PF00069	0.460
MOD_NEK2_1	585	590	PF00069	0.246
MOD_NEK2_1	629	634	PF00069	0.298
MOD_NEK2_1	717	722	PF00069	0.365
MOD_NEK2_1	816	821	PF00069	0.490
MOD_NEK2_1	842	847	PF00069	0.520
MOD_NEK2_1	852	857	PF00069	0.403
MOD_NEK2_1	90	95	PF00069	0.557
MOD_PIKK_1	331	337	PF00454	0.695
MOD_PIKK_1	483	489	PF00454	0.737
MOD_PIKK_1	589	595	PF00454	0.404
MOD_PKA_1	589	595	PF00069	0.338
MOD_PKA_2	248	254	PF00069	0.501
MOD_PKA_2	292	298	PF00069	0.687
MOD_PKA_2	589	595	PF00069	0.338
MOD_PKA_2	610	616	PF00069	0.457
MOD_PKA_2	638	644	PF00069	0.421
MOD_PKA_2	681	687	PF00069	0.462
MOD_PKA_2	72	78	PF00069	0.516
MOD_PKA_2	792	798	PF00069	0.760
MOD_Plk_1	269	275	PF00069	0.512
MOD_Plk_1	440	446	PF00069	0.453
MOD_Plk_1	717	723	PF00069	0.400
MOD_Plk_2-3	792	798	PF00069	0.763
MOD_Plk_4	215	221	PF00069	0.600
MOD_Plk_4	264	270	PF00069	0.461
MOD_Plk_4	441	447	PF00069	0.430
MOD_Plk_4	624	630	PF00069	0.408
MOD_Plk_4	66	72	PF00069	0.435
MOD_Plk_4	681	687	PF00069	0.469
MOD_Plk_4	712	718	PF00069	0.316
MOD_Plk_4	816	822	PF00069	0.479
MOD_ProDKin_1	125	131	PF00069	0.467
MOD_ProDKin_1	210	216	PF00069	0.739
MOD_ProDKin_1	388	394	PF00069	0.557
MOD_ProDKin_1	42	48	PF00069	0.638
MOD_ProDKin_1	554	560	PF00069	0.422
MOD_ProDKin_1	618	624	PF00069	0.448
MOD_ProDKin_1	733	739	PF00069	0.549
MOD_ProDKin_1	759	765	PF00069	0.557
MOD_SUMO_for_1	396	399	PF00179	0.585
MOD_SUMO_rev_2	231	237	PF00179	0.441
TRG_DiLeu_BaEn_1	808	813	PF01217	0.599
TRG_DiLeu_BaEn_4	665	671	PF01217	0.380
TRG_DiLeu_BaLyEn_6	349	354	PF01217	0.534
TRG_ENDOCYTIC_2	112	115	PF00928	0.514
TRG_ENDOCYTIC_2	538	541	PF00928	0.334
TRG_ENDOCYTIC_2	674	677	PF00928	0.357
TRG_ER_diArg_1	137	140	PF00400	0.543
TRG_ER_diArg_1	361	363	PF00400	0.424
TRG_ER_diArg_1	457	460	PF00400	0.550
TRG_ER_diArg_1	516	519	PF00400	0.631
TRG_ER_diArg_1	530	532	PF00400	0.592
TRG_ER_diArg_1	589	591	PF00400	0.330
TRG_NES_CRM1_1	68	83	PF08389	0.542
TRG_NES_CRM1_1	712	727	PF08389	0.427
TRG_NES_CRM1_1	851	866	PF08389	0.463
TRG_Pf-PMV_PEXEL_1	15	19	PF00026	0.487
TRG_Pf-PMV_PEXEL_1	532	536	PF00026	0.528
TRG_Pf-PMV_PEXEL_1	589	593	PF00026	0.443

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I206	Leptomonas seymouri	54%	95%
A0A0S4J0P6	Bodo saltans	30%	100%
A0A1X0P5D0	Trypanosomatidae	36%	100%
A0A3S7X9N5	Leishmania donovani	94%	100%
A0A422MYM3	Trypanosoma rangeli	37%	100%
A4HN46	Leishmania braziliensis	80%	97%
A4IBR4	Leishmania infantum	94%	100%
C9ZYV9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	36%	100%
V5BTC2	Trypanosoma cruzi	37%	100%