Uncharacterized protein

Quick info Localization Expansion Sequence features Structure Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania major

UniProt:

E9AFJ6_LEIMA

TriTrypDb:

LmjF.35.3720 , LMJLV39_350044800 , LMJSD75_350044200

Length:

440

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005856	cytoskeleton	5	2
GO:0043226	organelle	2	2
GO:0043228	non-membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043232	intracellular non-membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9AFJ6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFJ6

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	225	229	PF00656	0.616
CLV_C14_Caspase3-7	68	72	PF00656	0.634
CLV_NRD_NRD_1	184	186	PF00675	0.542
CLV_NRD_NRD_1	258	260	PF00675	0.502
CLV_NRD_NRD_1	276	278	PF00675	0.509
CLV_NRD_NRD_1	345	347	PF00675	0.526
CLV_NRD_NRD_1	431	433	PF00675	0.718
CLV_NRD_NRD_1	85	87	PF00675	0.502
CLV_PCSK_KEX2_1	128	130	PF00082	0.711
CLV_PCSK_KEX2_1	184	186	PF00082	0.545
CLV_PCSK_KEX2_1	210	212	PF00082	0.478
CLV_PCSK_KEX2_1	258	260	PF00082	0.502
CLV_PCSK_KEX2_1	276	278	PF00082	0.509
CLV_PCSK_KEX2_1	394	396	PF00082	0.617
CLV_PCSK_KEX2_1	85	87	PF00082	0.504
CLV_PCSK_PC1ET2_1	128	130	PF00082	0.711
CLV_PCSK_PC1ET2_1	210	212	PF00082	0.527
CLV_PCSK_PC1ET2_1	394	396	PF00082	0.617
CLV_PCSK_SKI1_1	169	173	PF00082	0.541
CLV_PCSK_SKI1_1	243	247	PF00082	0.536
CLV_PCSK_SKI1_1	300	304	PF00082	0.641
CLV_PCSK_SKI1_1	347	351	PF00082	0.536
CLV_Separin_Metazoa	338	342	PF03568	0.590
CLV_Separin_Metazoa	374	378	PF03568	0.416
DEG_Nend_UBRbox_2	1	3	PF02207	0.725
DOC_MAPK_gen_1	166	174	PF00069	0.512
DOC_MAPK_gen_1	184	191	PF00069	0.477
DOC_MAPK_gen_1	346	352	PF00069	0.520
DOC_PP1_RVXF_1	127	134	PF00149	0.690
DOC_USP7_MATH_1	305	309	PF00917	0.622
LIG_Actin_WH2_2	66	83	PF00022	0.505
LIG_CaM_IQ_9	73	88	PF13499	0.529
LIG_FHA_1	16	22	PF00498	0.619
LIG_FHA_1	280	286	PF00498	0.615
LIG_FHA_1	327	333	PF00498	0.505
LIG_FHA_2	223	229	PF00498	0.599
LIG_LIR_Gen_1	130	141	PF02991	0.617
LIG_LIR_Gen_1	186	194	PF02991	0.585
LIG_LIR_Nem_3	130	136	PF02991	0.634
LIG_LIR_Nem_3	186	191	PF02991	0.547
LIG_LIR_Nem_3	322	327	PF02991	0.516
LIG_LIR_Nem_3	328	333	PF02991	0.484
LIG_Rb_LxCxE_1	233	252	PF01857	0.631
LIG_SH2_CRK	188	192	PF00017	0.518
LIG_SH2_NCK_1	54	58	PF00017	0.651
LIG_SH2_PTP2	324	327	PF00017	0.597
LIG_SH2_SRC	324	327	PF00017	0.597
LIG_SH2_STAP1	24	28	PF00017	0.494
LIG_SH2_STAT5	324	327	PF00017	0.539
LIG_SUMO_SIM_par_1	170	175	PF11976	0.598
LIG_SUMO_SIM_par_1	348	353	PF11976	0.532
LIG_TRAF2_1	10	13	PF00917	0.574
LIG_TRAF2_1	20	23	PF00917	0.547
LIG_TRAF2_1	308	311	PF00917	0.624
LIG_TRAF2_1	95	98	PF00917	0.598
LIG_UBA3_1	349	354	PF00899	0.539
LIG_WRC_WIRS_1	327	332	PF05994	0.646
MOD_CK1_1	152	158	PF00069	0.601
MOD_CK1_1	160	166	PF00069	0.616
MOD_CK1_1	360	366	PF00069	0.500
MOD_CK2_1	131	137	PF00069	0.574
MOD_CK2_1	144	150	PF00069	0.423
MOD_CK2_1	238	244	PF00069	0.639
MOD_CK2_1	281	287	PF00069	0.548
MOD_CK2_1	305	311	PF00069	0.635
MOD_Cter_Amidation	126	129	PF01082	0.708
MOD_GlcNHglycan	174	177	PF01048	0.623
MOD_GlcNHglycan	228	231	PF01048	0.463
MOD_GlcNHglycan	300	303	PF01048	0.594
MOD_GlcNHglycan	305	308	PF01048	0.585
MOD_GlcNHglycan	384	387	PF01048	0.657
MOD_GlcNHglycan	388	391	PF01048	0.680
MOD_GlcNHglycan	435	438	PF01048	0.749
MOD_GSK3_1	152	159	PF00069	0.619
MOD_GSK3_1	222	229	PF00069	0.611
MOD_GSK3_1	277	284	PF00069	0.527
MOD_GSK3_1	353	360	PF00069	0.602
MOD_GSK3_1	378	385	PF00069	0.598
MOD_N-GLC_2	193	195	PF02516	0.599
MOD_NEK2_1	119	124	PF00069	0.558
MOD_NEK2_1	167	172	PF00069	0.612
MOD_NEK2_1	339	344	PF00069	0.564
MOD_NEK2_1	378	383	PF00069	0.595
MOD_NEK2_1	411	416	PF00069	0.545
MOD_NEK2_2	326	331	PF00069	0.641
MOD_PKA_2	15	21	PF00069	0.645
MOD_PKA_2	183	189	PF00069	0.554
MOD_PKA_2	411	417	PF00069	0.604
MOD_Plk_1	144	150	PF00069	0.531
MOD_Plk_1	167	173	PF00069	0.601
MOD_Plk_1	232	238	PF00069	0.588
MOD_Plk_1	378	384	PF00069	0.650
MOD_Plk_2-3	34	40	PF00069	0.607
MOD_Plk_4	114	120	PF00069	0.592
MOD_Plk_4	144	150	PF00069	0.593
MOD_Plk_4	167	173	PF00069	0.525
MOD_Plk_4	353	359	PF00069	0.623
MOD_SUMO_for_1	223	226	PF00179	0.603
MOD_SUMO_rev_2	150	160	PF00179	0.549
MOD_SUMO_rev_2	247	256	PF00179	0.544
MOD_SUMO_rev_2	398	405	PF00179	0.553
TRG_DiLeu_BaEn_1	400	405	PF01217	0.555
TRG_DiLeu_BaLyEn_6	328	333	PF01217	0.533
TRG_ENDOCYTIC_2	188	191	PF00928	0.488
TRG_ENDOCYTIC_2	324	327	PF00928	0.490
TRG_ER_diArg_1	184	187	PF00400	0.554
TRG_ER_diArg_1	276	278	PF00400	0.648
TRG_NES_CRM1_1	71	84	PF08389	0.560
TRG_Pf-PMV_PEXEL_1	19	23	PF00026	0.595
TRG_Pf-PMV_PEXEL_1	283	287	PF00026	0.552
TRG_Pf-PMV_PEXEL_1	395	400	PF00026	0.524
TRG_Pf-PMV_PEXEL_1	49	53	PF00026	0.519
TRG_Pf-PMV_PEXEL_1	99	103	PF00026	0.593

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IJ51	Leptomonas seymouri	69%	100%
A0A0S4JDF0	Bodo saltans	38%	99%
A0A1X0P5H1	Trypanosomatidae	43%	100%
A0A3Q8IFE4	Leishmania donovani	93%	100%
A0A3R7N172	Trypanosoma rangeli	42%	100%
A4HN43	Leishmania braziliensis	81%	100%
A4IBR1	Leishmania infantum	93%	100%
C9ZYW3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	100%
E9B6Q0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
V5BXZ9	Trypanosoma cruzi	40%	100%