Putative DNA repair protein RAD2

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DNA repair protein RAD2, putative

Species:

Leishmania major

UniProt:

E9AFI3_LEIMA

TriTrypDb:

LmjF.35.3590 , LMJLV39_350043400 , LMJSD75_350042900

Length:

931

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 6
NetGPI	no	yes: 0, no: 6

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	7
GO:0043226	organelle	2	7
GO:0043227	membrane-bounded organelle	3	7
GO:0043229	intracellular organelle	3	7
GO:0043231	intracellular membrane-bounded organelle	4	7
GO:0110165	cellular anatomical entity	1	7

Expansion

Sequence features

E9AFI3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFI3

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	7
GO:0006259	DNA metabolic process	4	7
GO:0006281	DNA repair	5	7
GO:0006289	nucleotide-excision repair	6	7
GO:0006725	cellular aromatic compound metabolic process	3	7
GO:0006807	nitrogen compound metabolic process	2	7
GO:0006950	response to stress	2	7
GO:0006974	DNA damage response	4	7
GO:0008152	metabolic process	1	7
GO:0009987	cellular process	1	7
GO:0033554	cellular response to stress	3	7
GO:0034641	cellular nitrogen compound metabolic process	3	7
GO:0043170	macromolecule metabolic process	3	7
GO:0044237	cellular metabolic process	2	7
GO:0044238	primary metabolic process	2	7
GO:0044260	obsolete cellular macromolecule metabolic process	3	7
GO:0046483	heterocycle metabolic process	3	7
GO:0050896	response to stimulus	1	7
GO:0051716	cellular response to stimulus	2	7
GO:0071704	organic substance metabolic process	2	7
GO:0090304	nucleic acid metabolic process	4	7
GO:1901360	organic cyclic compound metabolic process	3	7

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	7
GO:0003677	DNA binding	4	7
GO:0003697	single-stranded DNA binding	5	7
GO:0003824	catalytic activity	1	7
GO:0004518	nuclease activity	4	7
GO:0004519	endonuclease activity	5	7
GO:0004520	DNA endonuclease activity	5	2
GO:0004536	DNA nuclease activity	4	2
GO:0005488	binding	1	7
GO:0016787	hydrolase activity	2	7
GO:0016788	hydrolase activity, acting on ester bonds	3	7
GO:0016888	endodeoxyribonuclease activity, producing 5'-phosphomonoesters	6	2
GO:0016893	endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters	6	2
GO:0017108	5'-flap endonuclease activity	7	2
GO:0048256	flap endonuclease activity	6	2
GO:0097159	organic cyclic compound binding	2	7
GO:0140097	catalytic activity, acting on DNA	3	2
GO:0140640	catalytic activity, acting on a nucleic acid	2	2
GO:1901363	heterocyclic compound binding	2	7
GO:0043167	ion binding	2	1
GO:0043169	cation binding	3	1
GO:0046872	metal ion binding	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	270	274	PF00656	0.576
CLV_C14_Caspase3-7	316	320	PF00656	0.773
CLV_C14_Caspase3-7	331	335	PF00656	0.534
CLV_C14_Caspase3-7	459	463	PF00656	0.794
CLV_C14_Caspase3-7	466	470	PF00656	0.732
CLV_NRD_NRD_1	103	105	PF00675	0.626
CLV_NRD_NRD_1	208	210	PF00675	0.793
CLV_NRD_NRD_1	215	217	PF00675	0.659
CLV_NRD_NRD_1	265	267	PF00675	0.689
CLV_NRD_NRD_1	486	488	PF00675	0.673
CLV_NRD_NRD_1	552	554	PF00675	0.661
CLV_NRD_NRD_1	601	603	PF00675	0.684
CLV_NRD_NRD_1	773	775	PF00675	0.411
CLV_NRD_NRD_1	870	872	PF00675	0.411
CLV_NRD_NRD_1	876	878	PF00675	0.411
CLV_NRD_NRD_1	88	90	PF00675	0.335
CLV_PCSK_FUR_1	213	217	PF00082	0.689
CLV_PCSK_KEX2_1	102	104	PF00082	0.623
CLV_PCSK_KEX2_1	190	192	PF00082	0.714
CLV_PCSK_KEX2_1	208	210	PF00082	0.705
CLV_PCSK_KEX2_1	215	217	PF00082	0.667
CLV_PCSK_KEX2_1	25	27	PF00082	0.346
CLV_PCSK_KEX2_1	265	267	PF00082	0.689
CLV_PCSK_KEX2_1	486	488	PF00082	0.673
CLV_PCSK_KEX2_1	551	553	PF00082	0.662
CLV_PCSK_KEX2_1	773	775	PF00082	0.411
CLV_PCSK_KEX2_1	876	878	PF00082	0.372
CLV_PCSK_KEX2_1	88	90	PF00082	0.336
CLV_PCSK_PC1ET2_1	102	104	PF00082	0.591
CLV_PCSK_PC1ET2_1	190	192	PF00082	0.714
CLV_PCSK_PC1ET2_1	25	27	PF00082	0.346
CLV_PCSK_PC7_1	204	210	PF00082	0.770
CLV_PCSK_SKI1_1	106	110	PF00082	0.576
CLV_PCSK_SKI1_1	59	63	PF00082	0.346
CLV_PCSK_SKI1_1	69	73	PF00082	0.346
CLV_PCSK_SKI1_1	694	698	PF00082	0.362
CLV_PCSK_SKI1_1	718	722	PF00082	0.411
CLV_PCSK_SKI1_1	774	778	PF00082	0.510
CLV_PCSK_SKI1_1	893	897	PF00082	0.585
CLV_PCSK_SKI1_1	907	911	PF00082	0.608
DEG_APCC_DBOX_1	485	493	PF00400	0.755
DEG_SPOP_SBC_1	322	326	PF00917	0.642
DEG_SPOP_SBC_1	417	421	PF00917	0.683
DEG_SPOP_SBC_1	439	443	PF00917	0.627
DEG_SPOP_SBC_1	504	508	PF00917	0.606
DEG_SPOP_SBC_1	607	611	PF00917	0.627
DEG_SPOP_SBC_1	762	766	PF00917	0.513
DEG_SPOP_SBC_1	77	81	PF00917	0.563
DOC_CYCLIN_yCln2_LP_2	402	405	PF00134	0.683
DOC_CYCLIN_yCln2_LP_2	858	864	PF00134	0.513
DOC_MAPK_DCC_7	551	561	PF00069	0.727
DOC_MAPK_gen_1	102	110	PF00069	0.597
DOC_MAPK_gen_1	551	559	PF00069	0.726
DOC_MAPK_gen_1	630	638	PF00069	0.711
DOC_MAPK_gen_1	773	779	PF00069	0.436
DOC_MAPK_MEF2A_6	552	561	PF00069	0.719
DOC_MAPK_MEF2A_6	630	638	PF00069	0.634
DOC_MAPK_RevD_3	13	26	PF00069	0.619
DOC_MAPK_RevD_3	200	216	PF00069	0.600
DOC_PP1_RVXF_1	60	66	PF00149	0.546
DOC_PP2B_LxvP_1	200	203	PF13499	0.798
DOC_PP2B_LxvP_1	363	366	PF13499	0.718
DOC_PP2B_LxvP_1	402	405	PF13499	0.683
DOC_PP4_FxxP_1	261	264	PF00568	0.646
DOC_PP4_FxxP_1	519	522	PF00568	0.628
DOC_PP4_FxxP_1	74	77	PF00568	0.563
DOC_USP7_MATH_1	125	129	PF00917	0.704
DOC_USP7_MATH_1	147	151	PF00917	0.705
DOC_USP7_MATH_1	177	181	PF00917	0.811
DOC_USP7_MATH_1	322	326	PF00917	0.734
DOC_USP7_MATH_1	417	421	PF00917	0.681
DOC_USP7_MATH_1	439	443	PF00917	0.651
DOC_USP7_MATH_1	451	455	PF00917	0.633
DOC_USP7_MATH_1	504	508	PF00917	0.812
DOC_USP7_MATH_1	723	727	PF00917	0.411
DOC_USP7_MATH_1	762	766	PF00917	0.513
DOC_USP7_MATH_1	77	81	PF00917	0.548
DOC_USP7_MATH_2	495	501	PF00917	0.729
DOC_WW_Pin1_4	368	373	PF00397	0.742
DOC_WW_Pin1_4	394	399	PF00397	0.727
DOC_WW_Pin1_4	409	414	PF00397	0.646
DOC_WW_Pin1_4	446	451	PF00397	0.640
DOC_WW_Pin1_4	654	659	PF00397	0.411
DOC_WW_Pin1_4	833	838	PF00397	0.411
DOC_WW_Pin1_4	857	862	PF00397	0.515
LIG_14-3-3_CanoR_1	551	556	PF00244	0.805
LIG_14-3-3_CanoR_1	614	619	PF00244	0.804
LIG_14-3-3_CanoR_1	781	787	PF00244	0.411
LIG_14-3-3_CanoR_1	88	92	PF00244	0.563
LIG_14-3-3_CanoR_1	885	895	PF00244	0.578
LIG_14-3-3_CanoR_1	93	101	PF00244	0.676
LIG_Actin_WH2_2	219	234	PF00022	0.656
LIG_BRCT_BRCA1_1	448	452	PF00533	0.788
LIG_CSL_BTD_1	363	366	PF09270	0.691
LIG_deltaCOP1_diTrp_1	477	483	PF00928	0.756
LIG_deltaCOP1_diTrp_1	844	851	PF00928	0.436
LIG_FHA_1	290	296	PF00498	0.635
LIG_FHA_1	306	312	PF00498	0.704
LIG_FHA_1	324	330	PF00498	0.585
LIG_FHA_1	374	380	PF00498	0.747
LIG_FHA_1	383	389	PF00498	0.636
LIG_FHA_1	410	416	PF00498	0.820
LIG_FHA_1	552	558	PF00498	0.724
LIG_FHA_1	575	581	PF00498	0.718
LIG_FHA_1	629	635	PF00498	0.672
LIG_FHA_1	688	694	PF00498	0.411
LIG_FHA_1	715	721	PF00498	0.411
LIG_FHA_1	731	737	PF00498	0.411
LIG_FHA_2	240	246	PF00498	0.594
LIG_FHA_2	329	335	PF00498	0.723
LIG_FHA_2	405	411	PF00498	0.711
LIG_FHA_2	751	757	PF00498	0.513
LIG_LIR_Apic_2	258	264	PF02991	0.641
LIG_LIR_Apic_2	516	522	PF02991	0.634
LIG_LIR_Gen_1	785	793	PF02991	0.411
LIG_LIR_Gen_1	835	843	PF02991	0.364
LIG_LIR_Gen_1	844	852	PF02991	0.513
LIG_LIR_LC3C_4	746	750	PF02991	0.389
LIG_LIR_Nem_3	11	16	PF02991	0.546
LIG_LIR_Nem_3	477	483	PF02991	0.719
LIG_LIR_Nem_3	537	543	PF02991	0.707
LIG_LIR_Nem_3	785	789	PF02991	0.411
LIG_LIR_Nem_3	835	841	PF02991	0.364
LIG_LIR_Nem_3	844	848	PF02991	0.433
LIG_MYND_1	400	404	PF01753	0.729
LIG_NRBOX	488	494	PF00104	0.725
LIG_NRBOX	5	11	PF00104	0.546
LIG_NRBOX	723	729	PF00104	0.411
LIG_Pex14_1	34	38	PF04695	0.546
LIG_REV1ctd_RIR_1	824	834	PF16727	0.436
LIG_SH2_CRK	540	544	PF00017	0.812
LIG_SH2_SRC	540	543	PF00017	0.692
LIG_SH2_STAP1	536	540	PF00017	0.585
LIG_SH2_STAT3	898	901	PF00017	0.536
LIG_SH2_STAT5	654	657	PF00017	0.411
LIG_SH2_STAT5	732	735	PF00017	0.389
LIG_SH3_3	13	19	PF00018	0.546
LIG_SH3_3	283	289	PF00018	0.678
LIG_SH3_3	447	453	PF00018	0.640
LIG_SH3_3	554	560	PF00018	0.692
LIG_SH3_3	577	583	PF00018	0.713
LIG_SH3_3	631	637	PF00018	0.628
LIG_SH3_3	652	658	PF00018	0.378
LIG_SH3_3	776	782	PF00018	0.411
LIG_SUMO_SIM_anti_2	342	348	PF11976	0.786
LIG_SUMO_SIM_par_1	294	299	PF11976	0.797
LIG_SUMO_SIM_par_1	342	348	PF11976	0.737
LIG_SUMO_SIM_par_1	576	581	PF11976	0.818
LIG_SUMO_SIM_par_1	642	648	PF11976	0.411
LIG_SUMO_SIM_par_1	719	726	PF11976	0.411
LIG_SxIP_EBH_1	411	422	PF03271	0.586
LIG_TRAF2_1	195	198	PF00917	0.790
LIG_TRAF2_1	457	460	PF00917	0.829
LIG_TRAF2_1	785	788	PF00917	0.474
LIG_TRFH_1	654	658	PF08558	0.411
LIG_WRC_WIRS_1	10	15	PF05994	0.546
LIG_WW_3	521	525	PF00397	0.639
LIG_WW_3	781	785	PF00397	0.436
MOD_CK1_1	299	305	PF00069	0.749
MOD_CK1_1	324	330	PF00069	0.708
MOD_CK1_1	368	374	PF00069	0.768
MOD_CK1_1	392	398	PF00069	0.711
MOD_CK1_1	409	415	PF00069	0.737
MOD_CK1_1	418	424	PF00069	0.744
MOD_CK1_1	441	447	PF00069	0.821
MOD_CK1_1	454	460	PF00069	0.625
MOD_CK1_1	506	512	PF00069	0.764
MOD_CK1_1	609	615	PF00069	0.697
MOD_CK2_1	151	157	PF00069	0.573
MOD_CK2_1	239	245	PF00069	0.588
MOD_CK2_1	311	317	PF00069	0.741
MOD_CK2_1	330	336	PF00069	0.723
MOD_CK2_1	404	410	PF00069	0.706
MOD_CK2_1	427	433	PF00069	0.800
MOD_CK2_1	454	460	PF00069	0.824
MOD_CK2_1	654	660	PF00069	0.353
MOD_CK2_1	750	756	PF00069	0.416
MOD_CK2_1	782	788	PF00069	0.474
MOD_Cter_Amidation	188	191	PF01082	0.711
MOD_Cter_Amidation	23	26	PF01082	0.346
MOD_GlcNHglycan	128	131	PF01048	0.644
MOD_GlcNHglycan	153	156	PF01048	0.662
MOD_GlcNHglycan	179	182	PF01048	0.749
MOD_GlcNHglycan	183	186	PF01048	0.796
MOD_GlcNHglycan	222	225	PF01048	0.660
MOD_GlcNHglycan	280	283	PF01048	0.704
MOD_GlcNHglycan	298	301	PF01048	0.704
MOD_GlcNHglycan	367	370	PF01048	0.740
MOD_GlcNHglycan	372	375	PF01048	0.707
MOD_GlcNHglycan	380	383	PF01048	0.629
MOD_GlcNHglycan	423	426	PF01048	0.793
MOD_GlcNHglycan	429	432	PF01048	0.740
MOD_GlcNHglycan	508	511	PF01048	0.659
MOD_GlcNHglycan	580	583	PF01048	0.688
MOD_GlcNHglycan	603	606	PF01048	0.843
MOD_GlcNHglycan	711	714	PF01048	0.416
MOD_GlcNHglycan	756	759	PF01048	0.391
MOD_GlcNHglycan	765	768	PF01048	0.321
MOD_GSK3_1	147	154	PF00069	0.765
MOD_GSK3_1	177	184	PF00069	0.801
MOD_GSK3_1	220	227	PF00069	0.639
MOD_GSK3_1	311	318	PF00069	0.679
MOD_GSK3_1	324	331	PF00069	0.736
MOD_GSK3_1	364	371	PF00069	0.803
MOD_GSK3_1	378	385	PF00069	0.601
MOD_GSK3_1	411	418	PF00069	0.723
MOD_GSK3_1	421	428	PF00069	0.679
MOD_GSK3_1	434	441	PF00069	0.747
MOD_GSK3_1	451	458	PF00069	0.561
MOD_GSK3_1	499	506	PF00069	0.746
MOD_GSK3_1	522	529	PF00069	0.745
MOD_GSK3_1	547	554	PF00069	0.758
MOD_GSK3_1	574	581	PF00069	0.813
MOD_GSK3_1	609	616	PF00069	0.717
MOD_GSK3_1	750	757	PF00069	0.397
MOD_N-GLC_1	574	579	PF02516	0.689
MOD_NEK2_1	151	156	PF00069	0.591
MOD_NEK2_1	161	166	PF00069	0.677
MOD_NEK2_1	175	180	PF00069	0.749
MOD_NEK2_1	253	258	PF00069	0.655
MOD_NEK2_1	391	396	PF00069	0.666
MOD_NEK2_1	561	566	PF00069	0.818
MOD_NEK2_1	697	702	PF00069	0.411
MOD_NEK2_1	9	14	PF00069	0.535
MOD_PIKK_1	224	230	PF00454	0.647
MOD_PIKK_1	299	305	PF00454	0.753
MOD_PIKK_1	389	395	PF00454	0.815
MOD_PIKK_1	481	487	PF00454	0.717
MOD_PK_1	411	417	PF00069	0.634
MOD_PKA_1	551	557	PF00069	0.650
MOD_PKA_2	181	187	PF00069	0.754
MOD_PKA_2	444	450	PF00069	0.692
MOD_PKA_2	551	557	PF00069	0.630
MOD_PKA_2	601	607	PF00069	0.691
MOD_PKA_2	613	619	PF00069	0.642
MOD_PKA_2	87	93	PF00069	0.563
MOD_PKA_2	884	890	PF00069	0.420
MOD_PKB_1	191	199	PF00069	0.780
MOD_Plk_1	29	35	PF00069	0.546
MOD_Plk_1	382	388	PF00069	0.685
MOD_Plk_1	525	531	PF00069	0.604
MOD_Plk_1	574	580	PF00069	0.703
MOD_Plk_1	706	712	PF00069	0.513
MOD_Plk_2-3	315	321	PF00069	0.786
MOD_Plk_2-3	336	342	PF00069	0.817
MOD_Plk_2-3	455	461	PF00069	0.750
MOD_Plk_4	226	232	PF00069	0.712
MOD_Plk_4	256	262	PF00069	0.634
MOD_Plk_4	29	35	PF00069	0.614
MOD_Plk_4	411	417	PF00069	0.751
MOD_Plk_4	716	722	PF00069	0.507
MOD_Plk_4	723	729	PF00069	0.411
MOD_Plk_4	791	797	PF00069	0.436
MOD_ProDKin_1	368	374	PF00069	0.742
MOD_ProDKin_1	394	400	PF00069	0.726
MOD_ProDKin_1	409	415	PF00069	0.645
MOD_ProDKin_1	446	452	PF00069	0.642
MOD_ProDKin_1	654	660	PF00069	0.411
MOD_ProDKin_1	833	839	PF00069	0.411
MOD_ProDKin_1	857	863	PF00069	0.515
MOD_SUMO_for_1	138	141	PF00179	0.524
MOD_SUMO_rev_2	117	126	PF00179	0.583
MOD_SUMO_rev_2	46	53	PF00179	0.530
MOD_SUMO_rev_2	824	831	PF00179	0.436
TRG_DiLeu_BaEn_1	342	347	PF01217	0.839
TRG_DiLeu_BaEn_4	197	203	PF01217	0.731
TRG_DiLeu_BaLyEn_6	5	10	PF01217	0.539
TRG_ENDOCYTIC_2	540	543	PF00928	0.809
TRG_ER_diArg_1	103	106	PF00400	0.661
TRG_ER_diArg_1	212	215	PF00400	0.684
TRG_ER_diArg_1	264	266	PF00400	0.690
TRG_ER_diArg_1	38	41	PF00400	0.546
TRG_ER_diArg_1	551	553	PF00400	0.584
TRG_ER_diArg_1	772	774	PF00400	0.411
TRG_ER_diArg_1	847	850	PF00400	0.513
TRG_ER_diArg_1	875	877	PF00400	0.513
TRG_ER_diArg_1	904	907	PF00400	0.622
TRG_NLS_Bipartite_1	88	106	PF00514	0.680
TRG_NLS_MonoExtC_3	101	106	PF00514	0.585
TRG_Pf-PMV_PEXEL_1	215	220	PF00026	0.654
TRG_Pf-PMV_PEXEL_1	487	491	PF00026	0.596
TRG_Pf-PMV_PEXEL_1	809	813	PF00026	0.374
TRG_Pf-PMV_PEXEL_1	893	897	PF00026	0.585

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P4Y4	Leptomonas seymouri	49%	99%
A0A3S5H802	Leishmania donovani	90%	100%
A4HN29	Leishmania braziliensis	69%	90%
A4IBP8	Leishmania infantum	90%	100%
E9B6N8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	87%	100%