Multidrug and toxic compound extrusion protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

membrane transporter protein, putative

Species:

Leishmania major

UniProt:

E9AFI2_LEIMA

TriTrypDb:

LmjF.35.3580 , LMJLV39_350043300 , LMJSD75_350042800

Length:

704

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 17
NetGPI	no	yes: 0, no: 17

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	18
GO:0110165	cellular anatomical entity	1	18

Expansion

Sequence features

E9AFI2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFI2

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	10
GO:0009987	cellular process	1	10
GO:0042908	xenobiotic transport	4	10
GO:0046618	xenobiotic export from cell	3	10
GO:0051179	localization	1	10
GO:0051234	establishment of localization	2	10
GO:0055085	transmembrane transport	2	10
GO:0140115	export across plasma membrane	3	10
GO:0140352	export from cell	2	10
GO:1990961	xenobiotic detoxification by transmembrane export across the plasma membrane	4	10

Molecular functions
Term	Name	Level	Count
GO:0005215	transporter activity	1	18
GO:0015291	secondary active transmembrane transporter activity	4	18
GO:0015297	antiporter activity	5	18
GO:0022804	active transmembrane transporter activity	3	18
GO:0022857	transmembrane transporter activity	2	18
GO:0042910	xenobiotic transmembrane transporter activity	3	18

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	185	187	PF00675	0.225
CLV_NRD_NRD_1	334	336	PF00675	0.326
CLV_NRD_NRD_1	341	343	PF00675	0.299
CLV_NRD_NRD_1	383	385	PF00675	0.322
CLV_NRD_NRD_1	576	578	PF00675	0.432
CLV_NRD_NRD_1	636	638	PF00675	0.530
CLV_NRD_NRD_1	672	674	PF00675	0.515
CLV_PCSK_KEX2_1	185	187	PF00082	0.225
CLV_PCSK_KEX2_1	341	343	PF00082	0.275
CLV_PCSK_KEX2_1	576	578	PF00082	0.432
CLV_PCSK_KEX2_1	636	638	PF00082	0.406
CLV_PCSK_KEX2_1	672	674	PF00082	0.594
CLV_PCSK_SKI1_1	128	132	PF00082	0.216
CLV_PCSK_SKI1_1	269	273	PF00082	0.408
CLV_PCSK_SKI1_1	342	346	PF00082	0.298
CLV_PCSK_SKI1_1	384	388	PF00082	0.307
CLV_PCSK_SKI1_1	576	580	PF00082	0.467
CLV_Separin_Metazoa	182	186	PF03568	0.268
DEG_APCC_DBOX_1	127	135	PF00400	0.420
DEG_APCC_DBOX_1	168	176	PF00400	0.399
DEG_APCC_DBOX_1	341	349	PF00400	0.431
DEG_APCC_DBOX_1	506	514	PF00400	0.654
DEG_Nend_UBRbox_3	1	3	PF02207	0.644
DEG_SCF_FBW7_1	498	504	PF00400	0.571
DEG_SPOP_SBC_1	642	646	PF00917	0.609
DOC_CKS1_1	498	503	PF01111	0.571
DOC_CYCLIN_yClb5_NLxxxL_5	354	363	PF00134	0.269
DOC_CYCLIN_yCln2_LP_2	49	55	PF00134	0.354
DOC_MAPK_gen_1	338	348	PF00069	0.372
DOC_MAPK_gen_1	384	391	PF00069	0.187
DOC_MAPK_MEF2A_6	128	136	PF00069	0.452
DOC_MAPK_MEF2A_6	341	350	PF00069	0.550
DOC_MAPK_MEF2A_6	384	391	PF00069	0.433
DOC_MAPK_MEF2A_6	48	55	PF00069	0.454
DOC_MAPK_NFAT4_5	128	136	PF00069	0.462
DOC_MAPK_NFAT4_5	48	56	PF00069	0.524
DOC_PP1_RVXF_1	215	222	PF00149	0.366
DOC_PP1_RVXF_1	267	273	PF00149	0.404
DOC_PP2B_LxvP_1	49	52	PF13499	0.432
DOC_PP2B_LxvP_1	579	582	PF13499	0.717
DOC_PP2B_LxvP_1	639	642	PF13499	0.742
DOC_PP4_FxxP_1	139	142	PF00568	0.333
DOC_USP7_MATH_1	271	275	PF00917	0.330
DOC_USP7_MATH_1	501	505	PF00917	0.639
DOC_USP7_MATH_1	589	593	PF00917	0.704
DOC_USP7_MATH_1	59	63	PF00917	0.399
DOC_USP7_MATH_1	613	617	PF00917	0.699
DOC_USP7_MATH_1	680	684	PF00917	0.757
DOC_WW_Pin1_4	34	39	PF00397	0.589
DOC_WW_Pin1_4	497	502	PF00397	0.661
DOC_WW_Pin1_4	534	539	PF00397	0.722
DOC_WW_Pin1_4	547	552	PF00397	0.700
DOC_WW_Pin1_4	570	575	PF00397	0.768
DOC_WW_Pin1_4	608	613	PF00397	0.595
DOC_WW_Pin1_4	618	623	PF00397	0.771
DOC_WW_Pin1_4	637	642	PF00397	0.783
DOC_WW_Pin1_4	647	652	PF00397	0.656
LIG_14-3-3_CanoR_1	169	173	PF00244	0.403
LIG_14-3-3_CanoR_1	576	582	PF00244	0.738
LIG_14-3-3_CanoR_1	636	640	PF00244	0.639
LIG_14-3-3_CanoR_1	667	672	PF00244	0.773
LIG_Actin_WH2_2	255	271	PF00022	0.420
LIG_Actin_WH2_2	35	50	PF00022	0.473
LIG_APCC_ABBA_1	475	480	PF00400	0.600
LIG_BIR_III_2	609	613	PF00653	0.595
LIG_BRCT_BRCA1_1	317	321	PF00533	0.312
LIG_Clathr_ClatBox_1	178	182	PF01394	0.269
LIG_eIF4E_1	390	396	PF01652	0.269
LIG_EVH1_2	6	10	PF00568	0.595
LIG_FHA_1	116	122	PF00498	0.538
LIG_FHA_1	239	245	PF00498	0.444
LIG_FHA_1	360	366	PF00498	0.388
LIG_FHA_1	372	378	PF00498	0.269
LIG_FHA_1	39	45	PF00498	0.567
LIG_FHA_1	524	530	PF00498	0.665
LIG_FHA_1	535	541	PF00498	0.696
LIG_FHA_1	692	698	PF00498	0.747
LIG_IRF3_LxIS_1	146	153	PF10401	0.269
LIG_LIR_Apic_2	444	450	PF02991	0.287
LIG_LIR_Gen_1	164	175	PF02991	0.230
LIG_LIR_Gen_1	238	245	PF02991	0.444
LIG_LIR_Gen_1	301	309	PF02991	0.305
LIG_LIR_Gen_1	351	361	PF02991	0.418
LIG_LIR_Gen_1	471	479	PF02991	0.594
LIG_LIR_Gen_1	565	574	PF02991	0.713
LIG_LIR_Nem_3	164	170	PF02991	0.241
LIG_LIR_Nem_3	171	176	PF02991	0.268
LIG_LIR_Nem_3	238	243	PF02991	0.395
LIG_LIR_Nem_3	301	306	PF02991	0.307
LIG_LIR_Nem_3	351	357	PF02991	0.387
LIG_LIR_Nem_3	471	475	PF02991	0.577
LIG_LIR_Nem_3	565	569	PF02991	0.710
LIG_PCNA_yPIPBox_3	376	388	PF02747	0.231
LIG_PCNA_yPIPBox_3	400	408	PF02747	0.462
LIG_Pex14_2	163	167	PF04695	0.324
LIG_PTAP_UEV_1	502	507	PF05743	0.575
LIG_SH2_CRK	294	298	PF00017	0.324
LIG_SH2_CRK	424	428	PF00017	0.287
LIG_SH2_CRK	462	466	PF00017	0.347
LIG_SH2_NCK_1	294	298	PF00017	0.231
LIG_SH2_NCK_1	424	428	PF00017	0.287
LIG_SH2_SRC	294	297	PF00017	0.231
LIG_SH2_STAP1	176	180	PF00017	0.393
LIG_SH2_STAP1	95	99	PF00017	0.249
LIG_SH2_STAT5	187	190	PF00017	0.458
LIG_SH2_STAT5	242	245	PF00017	0.330
LIG_SH2_STAT5	390	393	PF00017	0.319
LIG_SH2_STAT5	464	467	PF00017	0.323
LIG_SH2_STAT5	469	472	PF00017	0.510
LIG_SH3_1	673	679	PF00018	0.607
LIG_SH3_3	1	7	PF00018	0.646
LIG_SH3_3	424	430	PF00018	0.330
LIG_SH3_3	454	460	PF00018	0.400
LIG_SH3_3	500	506	PF00018	0.630
LIG_SH3_3	583	589	PF00018	0.615
LIG_SH3_3	673	679	PF00018	0.746
LIG_SUMO_SIM_anti_2	177	182	PF11976	0.269
LIG_SUMO_SIM_anti_2	360	365	PF11976	0.351
LIG_SUMO_SIM_anti_2	62	67	PF11976	0.278
LIG_SUMO_SIM_par_1	40	45	PF11976	0.551
LIG_TRAF2_1	698	701	PF00917	0.612
LIG_TYR_ITIM	391	396	PF00017	0.269
LIG_TYR_ITIM	422	427	PF00017	0.347
LIG_UBA3_1	239	248	PF00899	0.312
LIG_WRC_WIRS_1	396	401	PF05994	0.354
LIG_WRC_WIRS_1	566	571	PF05994	0.742
LIG_WRC_WIRS_1	581	586	PF05994	0.724
MOD_CDC14_SPxK_1	640	643	PF00782	0.593
MOD_CDK_SPxK_1	570	576	PF00069	0.593
MOD_CDK_SPxK_1	637	643	PF00069	0.599
MOD_CDK_SPxxK_3	534	541	PF00069	0.581
MOD_CDK_SPxxK_3	570	577	PF00069	0.586
MOD_CK1_1	13	19	PF00069	0.702
MOD_CK1_1	238	244	PF00069	0.187
MOD_CK1_1	315	321	PF00069	0.375
MOD_CK1_1	398	404	PF00069	0.269
MOD_CK1_1	565	571	PF00069	0.727
MOD_CK1_1	580	586	PF00069	0.695
MOD_CK1_1	683	689	PF00069	0.812
MOD_CK2_1	485	491	PF00069	0.718
MOD_CK2_1	512	518	PF00069	0.748
MOD_CK2_1	539	545	PF00069	0.774
MOD_CK2_1	600	606	PF00069	0.648
MOD_CK2_1	656	662	PF00069	0.657
MOD_CMANNOS	253	256	PF00535	0.325
MOD_Cter_Amidation	634	637	PF01082	0.533
MOD_DYRK1A_RPxSP_1	637	641	PF00069	0.769
MOD_GlcNHglycan	19	22	PF01048	0.592
MOD_GlcNHglycan	317	320	PF01048	0.344
MOD_GlcNHglycan	503	506	PF01048	0.528
MOD_GlcNHglycan	552	555	PF01048	0.542
MOD_GlcNHglycan	626	629	PF01048	0.471
MOD_GlcNHglycan	651	654	PF01048	0.568
MOD_GlcNHglycan	656	659	PF01048	0.524
MOD_GlcNHglycan	673	676	PF01048	0.582
MOD_GlcNHglycan	682	685	PF01048	0.391
MOD_GlcNHglycan	97	100	PF01048	0.291
MOD_GSK3_1	146	153	PF00069	0.269
MOD_GSK3_1	226	233	PF00069	0.441
MOD_GSK3_1	277	284	PF00069	0.286
MOD_GSK3_1	308	315	PF00069	0.421
MOD_GSK3_1	34	41	PF00069	0.581
MOD_GSK3_1	481	488	PF00069	0.672
MOD_GSK3_1	497	504	PF00069	0.676
MOD_GSK3_1	519	526	PF00069	0.786
MOD_GSK3_1	530	537	PF00069	0.662
MOD_GSK3_1	539	546	PF00069	0.659
MOD_GSK3_1	6	13	PF00069	0.752
MOD_GSK3_1	637	644	PF00069	0.810
MOD_GSK3_1	645	652	PF00069	0.781
MOD_GSK3_1	667	674	PF00069	0.799
MOD_N-GLC_1	357	362	PF02516	0.269
MOD_N-GLC_1	562	567	PF02516	0.518
MOD_N-GLC_1	589	594	PF02516	0.454
MOD_N-GLC_1	618	623	PF02516	0.454
MOD_N-GLC_1	90	95	PF02516	0.317
MOD_N-GLC_2	570	572	PF02516	0.433
MOD_NEK2_1	10	15	PF00069	0.714
MOD_NEK2_1	109	114	PF00069	0.433
MOD_NEK2_1	146	151	PF00069	0.428
MOD_NEK2_1	230	235	PF00069	0.308
MOD_NEK2_1	243	248	PF00069	0.379
MOD_NEK2_1	348	353	PF00069	0.291
MOD_NEK2_1	359	364	PF00069	0.278
MOD_NEK2_1	395	400	PF00069	0.393
MOD_NEK2_1	422	427	PF00069	0.268
MOD_NEK2_1	436	441	PF00069	0.291
MOD_NEK2_1	452	457	PF00069	0.320
MOD_NEK2_1	47	52	PF00069	0.528
MOD_NEK2_1	543	548	PF00069	0.759
MOD_NEK2_1	600	605	PF00069	0.785
MOD_NEK2_1	671	676	PF00069	0.755
MOD_NEK2_1	80	85	PF00069	0.336
MOD_NEK2_1	90	95	PF00069	0.331
MOD_NEK2_2	168	173	PF00069	0.269
MOD_NEK2_2	349	354	PF00069	0.334
MOD_PIKK_1	107	113	PF00454	0.431
MOD_PIKK_1	192	198	PF00454	0.603
MOD_PIKK_1	281	287	PF00454	0.286
MOD_PIKK_1	686	692	PF00454	0.618
MOD_PKA_2	168	174	PF00069	0.363
MOD_PKA_2	530	536	PF00069	0.699
MOD_PKA_2	635	641	PF00069	0.758
MOD_PKA_2	666	672	PF00069	0.774
MOD_PKA_2	686	692	PF00069	0.644
MOD_Plk_1	357	363	PF00069	0.269
MOD_Plk_1	490	496	PF00069	0.700
MOD_Plk_1	562	568	PF00069	0.729
MOD_Plk_1	90	96	PF00069	0.268
MOD_Plk_4	104	110	PF00069	0.317
MOD_Plk_4	146	152	PF00069	0.364
MOD_Plk_4	174	180	PF00069	0.357
MOD_Plk_4	235	241	PF00069	0.301
MOD_Plk_4	308	314	PF00069	0.341
MOD_Plk_4	349	355	PF00069	0.279
MOD_Plk_4	359	365	PF00069	0.262
MOD_Plk_4	368	374	PF00069	0.320
MOD_Plk_4	422	428	PF00069	0.332
MOD_Plk_4	452	458	PF00069	0.315
MOD_Plk_4	691	697	PF00069	0.612
MOD_Plk_4	80	86	PF00069	0.231
MOD_ProDKin_1	34	40	PF00069	0.589
MOD_ProDKin_1	497	503	PF00069	0.661
MOD_ProDKin_1	534	540	PF00069	0.724
MOD_ProDKin_1	547	553	PF00069	0.699
MOD_ProDKin_1	570	576	PF00069	0.768
MOD_ProDKin_1	608	614	PF00069	0.595
MOD_ProDKin_1	618	624	PF00069	0.771
MOD_ProDKin_1	637	643	PF00069	0.786
MOD_ProDKin_1	647	653	PF00069	0.657
TRG_DiLeu_BaEn_1	257	262	PF01217	0.478
TRG_DiLeu_BaEn_1	323	328	PF01217	0.399
TRG_DiLeu_BaLyEn_6	263	268	PF01217	0.479
TRG_DiLeu_BaLyEn_6	595	600	PF01217	0.646
TRG_ENDOCYTIC_2	176	179	PF00928	0.316
TRG_ENDOCYTIC_2	242	245	PF00928	0.254
TRG_ENDOCYTIC_2	294	297	PF00928	0.347
TRG_ENDOCYTIC_2	393	396	PF00928	0.330
TRG_ENDOCYTIC_2	424	427	PF00928	0.301
TRG_ENDOCYTIC_2	462	465	PF00928	0.319
TRG_ER_diArg_1	184	186	PF00400	0.425
TRG_ER_diArg_1	671	673	PF00400	0.618
TRG_Pf-PMV_PEXEL_1	541	545	PF00026	0.538

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I213	Leptomonas seymouri	38%	100%
A0A0N1IJ56	Leptomonas seymouri	59%	100%
A0A0S4INI5	Bodo saltans	39%	100%
A0A0S4JV08	Bodo saltans	23%	100%
A0A0S4JZ61	Bodo saltans	24%	100%
A0A1X0NU76	Trypanosomatidae	48%	100%
A0A3Q8IGH9	Leishmania donovani	89%	100%
A0A3Q8II95	Leishmania donovani	39%	100%
A0A3R7M3E2	Trypanosoma rangeli	49%	100%
A0A3R7NUJ8	Trypanosoma rangeli	44%	100%
A4HN33	Leishmania braziliensis	39%	100%
A4HN34	Leishmania braziliensis	40%	100%
A4IBQ2	Leishmania infantum	39%	100%
A7KAU2	Oryctolagus cuniculus	30%	100%
D0A551	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	24%	100%
E9AFI7	Leishmania major	40%	100%
E9AHW7	Leishmania infantum	89%	100%
E9B6N7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	83%	100%
E9B6P2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	39%	100%
F4I4Q3	Arabidopsis thaliana	23%	100%
F4JH46	Arabidopsis thaliana	26%	100%
O82752	Arabidopsis thaliana	26%	100%
Q5RFD2	Pongo abelii	31%	100%
Q6NB79	Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)	23%	100%
Q86VL8	Homo sapiens	27%	100%
Q8L616	Arabidopsis thaliana	23%	100%
Q96FL8	Homo sapiens	31%	100%
Q9FHB6	Arabidopsis thaliana	28%	100%
Q9LS19	Arabidopsis thaliana	25%	100%
Q9LYT3	Arabidopsis thaliana	28%	100%
Q9UT92	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	24%	100%
V5BJE3	Trypanosoma cruzi	47%	100%