PKS_AT domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

acyl transferase-like protein, putative

Species:

Leishmania major

UniProt:

E9AFH1_LEIMA

TriTrypDb:

LmjF.35.3470 , LMJLV39_350042100 , LMJSD75_350041600

Length:

532

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005835	fatty acid synthase complex	3	2
GO:0032991	protein-containing complex	1	2
GO:0140535	intracellular protein-containing complex	2	2
GO:1902494	catalytic complex	2	2

Expansion

Sequence features

E9AFH1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFH1

Function

Biological processes
Term	Name	Level	Count
GO:0006082	organic acid metabolic process	3	4
GO:0006629	lipid metabolic process	3	4
GO:0006631	fatty acid metabolic process	4	4
GO:0006633	fatty acid biosynthetic process	5	4
GO:0008152	metabolic process	1	4
GO:0008610	lipid biosynthetic process	4	4
GO:0009058	biosynthetic process	2	4
GO:0009987	cellular process	1	4
GO:0016053	organic acid biosynthetic process	4	4
GO:0019752	carboxylic acid metabolic process	5	4
GO:0032787	monocarboxylic acid metabolic process	6	4
GO:0043436	oxoacid metabolic process	4	4
GO:0044237	cellular metabolic process	2	4
GO:0044238	primary metabolic process	2	4
GO:0044249	cellular biosynthetic process	3	4
GO:0044255	cellular lipid metabolic process	3	4
GO:0044281	small molecule metabolic process	2	4
GO:0044283	small molecule biosynthetic process	3	4
GO:0046394	carboxylic acid biosynthetic process	5	4
GO:0071704	organic substance metabolic process	2	4
GO:0072330	monocarboxylic acid biosynthetic process	6	4
GO:1901576	organic substance biosynthetic process	3	4

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0004312	fatty acid synthase activity	5	4
GO:0004314	[acyl-carrier-protein] S-malonyltransferase activity	6	2
GO:0016417	S-acyltransferase activity	5	2
GO:0016419	S-malonyltransferase activity	6	2
GO:0016420	malonyltransferase activity	5	2
GO:0016740	transferase activity	2	11
GO:0016746	acyltransferase activity	3	4
GO:0016747	acyltransferase activity, transferring groups other than amino-acyl groups	4	4

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	275	279	PF00656	0.506
CLV_C14_Caspase3-7	91	95	PF00656	0.490
CLV_NRD_NRD_1	177	179	PF00675	0.393
CLV_NRD_NRD_1	307	309	PF00675	0.547
CLV_NRD_NRD_1	358	360	PF00675	0.421
CLV_NRD_NRD_1	394	396	PF00675	0.545
CLV_NRD_NRD_1	504	506	PF00675	0.550
CLV_PCSK_FUR_1	423	427	PF00082	0.354
CLV_PCSK_KEX2_1	161	163	PF00082	0.306
CLV_PCSK_KEX2_1	307	309	PF00082	0.511
CLV_PCSK_KEX2_1	358	360	PF00082	0.421
CLV_PCSK_KEX2_1	394	396	PF00082	0.593
CLV_PCSK_KEX2_1	425	427	PF00082	0.516
CLV_PCSK_KEX2_1	496	498	PF00082	0.511
CLV_PCSK_KEX2_1	504	506	PF00082	0.377
CLV_PCSK_KEX2_1	71	73	PF00082	0.409
CLV_PCSK_PC1ET2_1	161	163	PF00082	0.350
CLV_PCSK_PC1ET2_1	425	427	PF00082	0.483
CLV_PCSK_PC1ET2_1	496	498	PF00082	0.494
CLV_PCSK_PC1ET2_1	71	73	PF00082	0.423
CLV_PCSK_PC7_1	303	309	PF00082	0.504
CLV_PCSK_SKI1_1	162	166	PF00082	0.306
CLV_PCSK_SKI1_1	2	6	PF00082	0.369
CLV_PCSK_SKI1_1	308	312	PF00082	0.529
CLV_PCSK_SKI1_1	369	373	PF00082	0.401
CLV_PCSK_SKI1_1	379	383	PF00082	0.365
CLV_PCSK_SKI1_1	516	520	PF00082	0.509
DEG_Nend_UBRbox_1	1	4	PF02207	0.464
DEG_SPOP_SBC_1	213	217	PF00917	0.388
DOC_CYCLIN_RxL_1	159	167	PF00134	0.430
DOC_CYCLIN_RxL_1	376	385	PF00134	0.498
DOC_CYCLIN_yCln2_LP_2	22	28	PF00134	0.374
DOC_MAPK_gen_1	338	346	PF00069	0.432
DOC_MAPK_gen_1	394	401	PF00069	0.530
DOC_MAPK_gen_1	435	444	PF00069	0.560
DOC_MAPK_gen_1	514	522	PF00069	0.481
DOC_MAPK_MEF2A_6	339	348	PF00069	0.414
DOC_MAPK_MEF2A_6	514	522	PF00069	0.481
DOC_MAPK_RevD_3	147	162	PF00069	0.306
DOC_USP7_MATH_1	134	138	PF00917	0.313
DOC_USP7_MATH_1	213	217	PF00917	0.430
DOC_USP7_MATH_1	268	272	PF00917	0.428
DOC_USP7_MATH_1	293	297	PF00917	0.633
DOC_USP7_MATH_1	351	355	PF00917	0.347
DOC_USP7_MATH_1	58	62	PF00917	0.307
DOC_WW_Pin1_4	148	153	PF00397	0.325
DOC_WW_Pin1_4	181	186	PF00397	0.369
DOC_WW_Pin1_4	315	320	PF00397	0.530
DOC_WW_Pin1_4	491	496	PF00397	0.465
DOC_WW_Pin1_4	81	86	PF00397	0.388
LIG_14-3-3_CanoR_1	303	307	PF00244	0.525
LIG_14-3-3_CanoR_1	497	503	PF00244	0.459
LIG_14-3-3_CanoR_1	504	509	PF00244	0.398
LIG_14-3-3_CanoR_1	72	80	PF00244	0.376
LIG_Actin_WH2_2	105	121	PF00022	0.249
LIG_Actin_WH2_2	343	360	PF00022	0.418
LIG_BRCT_BRCA1_1	499	503	PF00533	0.505
LIG_BRCT_BRCA1_1	60	64	PF00533	0.399
LIG_FHA_1	316	322	PF00498	0.541
LIG_FHA_1	403	409	PF00498	0.491
LIG_FHA_1	439	445	PF00498	0.454
LIG_FHA_1	73	79	PF00498	0.423
LIG_FHA_1	85	91	PF00498	0.423
LIG_FHA_2	152	158	PF00498	0.368
LIG_FHA_2	194	200	PF00498	0.384
LIG_FHA_2	326	332	PF00498	0.561
LIG_FHA_2	474	480	PF00498	0.446
LIG_FHA_2	89	95	PF00498	0.423
LIG_LIR_Apic_2	341	345	PF02991	0.403
LIG_LIR_Gen_1	140	149	PF02991	0.306
LIG_LIR_Gen_1	199	209	PF02991	0.279
LIG_LIR_Gen_1	215	226	PF02991	0.356
LIG_LIR_LC3C_4	439	444	PF02991	0.464
LIG_LIR_Nem_3	140	144	PF02991	0.306
LIG_LIR_Nem_3	198	204	PF02991	0.327
LIG_LIR_Nem_3	215	221	PF02991	0.222
LIG_LIR_Nem_3	304	309	PF02991	0.528
LIG_LIR_Nem_3	341	346	PF02991	0.400
LIG_LIR_Nem_3	362	367	PF02991	0.359
LIG_LRP6_Inhibitor_1	221	227	PF00058	0.366
LIG_PALB2_WD40_1	338	346	PF16756	0.476
LIG_PCNA_TLS_4	358	365	PF02747	0.405
LIG_SH2_STAT3	101	104	PF00017	0.405
LIG_SH2_STAT3	126	129	PF00017	0.366
LIG_SH2_STAT5	101	104	PF00017	0.375
LIG_SH2_STAT5	231	234	PF00017	0.306
LIG_SH3_3	146	152	PF00018	0.325
LIG_SUMO_SIM_anti_2	215	223	PF11976	0.336
LIG_SUMO_SIM_par_1	439	446	PF11976	0.526
LIG_TRAF2_1	320	323	PF00917	0.584
LIG_TRAF2_1	328	331	PF00917	0.618
LIG_TRAF2_1	477	480	PF00917	0.477
LIG_TYR_ITIM	113	118	PF00017	0.436
LIG_UBA3_1	381	386	PF00899	0.491
LIG_WRC_WIRS_1	444	449	PF05994	0.509
MOD_CDC14_SPxK_1	184	187	PF00782	0.433
MOD_CDC14_SPxK_1	494	497	PF00782	0.493
MOD_CDK_SPK_2	491	496	PF00069	0.447
MOD_CDK_SPxK_1	181	187	PF00069	0.385
MOD_CDK_SPxK_1	491	497	PF00069	0.473
MOD_CK1_1	151	157	PF00069	0.403
MOD_CK1_1	191	197	PF00069	0.366
MOD_CK1_1	263	269	PF00069	0.544
MOD_CK1_1	302	308	PF00069	0.607
MOD_CK1_1	524	530	PF00069	0.511
MOD_CK2_1	134	140	PF00069	0.306
MOD_CK2_1	193	199	PF00069	0.380
MOD_CK2_1	214	220	PF00069	0.303
MOD_CK2_1	317	323	PF00069	0.565
MOD_CK2_1	325	331	PF00069	0.576
MOD_CK2_1	416	422	PF00069	0.512
MOD_CK2_1	473	479	PF00069	0.475
MOD_GlcNHglycan	256	259	PF01048	0.628
MOD_GlcNHglycan	295	298	PF01048	0.599
MOD_GlcNHglycan	334	337	PF01048	0.548
MOD_GlcNHglycan	418	421	PF01048	0.525
MOD_GlcNHglycan	448	451	PF01048	0.467
MOD_GlcNHglycan	7	10	PF01048	0.305
MOD_GSK3_1	208	215	PF00069	0.355
MOD_GSK3_1	263	270	PF00069	0.448
MOD_GSK3_1	520	527	PF00069	0.510
MOD_GSK3_1	80	87	PF00069	0.361
MOD_N-GLC_1	188	193	PF02516	0.407
MOD_N-GLC_1	402	407	PF02516	0.345
MOD_N-GLC_1	497	502	PF02516	0.501
MOD_NEK2_1	156	161	PF00069	0.363
MOD_NEK2_1	17	22	PF00069	0.150
MOD_NEK2_1	212	217	PF00069	0.311
MOD_NEK2_1	292	297	PF00069	0.503
MOD_NEK2_1	352	357	PF00069	0.356
MOD_NEK2_1	399	404	PF00069	0.390
MOD_NEK2_1	416	421	PF00069	0.323
MOD_NEK2_1	438	443	PF00069	0.529
MOD_NEK2_1	5	10	PF00069	0.311
MOD_NEK2_1	520	525	PF00069	0.446
MOD_NEK2_1	80	85	PF00069	0.382
MOD_NEK2_1	97	102	PF00069	0.251
MOD_NEK2_2	268	273	PF00069	0.516
MOD_PIKK_1	100	106	PF00454	0.348
MOD_PKA_1	338	344	PF00069	0.439
MOD_PKA_1	496	502	PF00069	0.460
MOD_PKA_1	504	510	PF00069	0.348
MOD_PKA_2	302	308	PF00069	0.524
MOD_PKA_2	485	491	PF00069	0.526
MOD_PKA_2	496	502	PF00069	0.462
MOD_PKA_2	504	510	PF00069	0.421
MOD_Plk_1	139	145	PF00069	0.306
MOD_Plk_1	156	162	PF00069	0.306
MOD_Plk_1	322	328	PF00069	0.577
MOD_Plk_1	438	444	PF00069	0.528
MOD_Plk_4	17	23	PF00069	0.403
MOD_Plk_4	214	220	PF00069	0.307
MOD_Plk_4	260	266	PF00069	0.499
MOD_Plk_4	338	344	PF00069	0.439
MOD_Plk_4	438	444	PF00069	0.531
MOD_Plk_4	504	510	PF00069	0.556
MOD_Plk_4	97	103	PF00069	0.362
MOD_ProDKin_1	148	154	PF00069	0.325
MOD_ProDKin_1	181	187	PF00069	0.369
MOD_ProDKin_1	315	321	PF00069	0.533
MOD_ProDKin_1	491	497	PF00069	0.473
MOD_ProDKin_1	81	87	PF00069	0.388
TRG_DiLeu_BaEn_4	322	328	PF01217	0.564
TRG_DiLeu_BaLyEn_6	342	347	PF01217	0.524
TRG_ENDOCYTIC_2	115	118	PF00928	0.433
TRG_ER_diArg_1	306	308	PF00400	0.509
TRG_ER_diArg_1	357	359	PF00400	0.400
TRG_ER_diArg_1	503	505	PF00400	0.547
TRG_Pf-PMV_PEXEL_1	162	167	PF00026	0.430

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IJM7	Leptomonas seymouri	67%	94%
A0A0S4IR28	Bodo saltans	38%	99%
A0A1X0P5F7	Trypanosomatidae	52%	97%
A0A3Q8IGH2	Leishmania donovani	94%	100%
A0A422NRA5	Trypanosoma rangeli	51%	99%
A4HN14	Leishmania braziliensis	81%	100%
A4IBN7	Leishmania infantum	95%	100%
C9ZYZ4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	51%	97%
E9B6M6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%