Replication protein A 14 kDa subunit

Quick info Annotations Localization Abundance Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

replication factor A protein 3, putative

Species:

Leishmania major

UniProt:

E9AFG8_LEIMA

TriTrypDb:

LmjF.35.3440 , LMJLV39_350041800 , LMJSD75_350041300

Length:

117

Annotations

Annotations by Jardim et al.

DNA replication, replication factor A 3

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0031974	membrane-enclosed lumen	2	11
GO:0031981	nuclear lumen	5	11
GO:0043233	organelle lumen	3	11
GO:0070013	intracellular organelle lumen	4	11
GO:0110165	cellular anatomical entity	1	11

Abundance

Amastigote (protein)


Source	Evidence on protein	Close homologs
Pescher et al. (upgregulation)	no	yes: 0

Promastigote and amastigote


Source	Evidence on protein	Close homologs
Lahav et al. - mRNA - Protein

Expansion

Sequence features

E9AFG8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFG8

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006259	DNA metabolic process	4	11
GO:0006260	DNA replication	5	11
GO:0006281	DNA repair	5	11
GO:0006310	DNA recombination	5	11
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0006950	response to stress	2	11
GO:0006974	DNA damage response	4	11
GO:0008152	metabolic process	1	11
GO:0009987	cellular process	1	11
GO:0033554	cellular response to stress	3	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0043170	macromolecule metabolic process	3	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044260	obsolete cellular macromolecule metabolic process	3	11
GO:0046483	heterocycle metabolic process	3	11
GO:0050896	response to stimulus	1	11
GO:0051716	cellular response to stimulus	2	11
GO:0071704	organic substance metabolic process	2	11
GO:0090304	nucleic acid metabolic process	4	11
GO:1901360	organic cyclic compound metabolic process	3	11

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	11
GO:0003677	DNA binding	4	11
GO:0005488	binding	1	11
GO:0097159	organic cyclic compound binding	2	11
GO:1901363	heterocyclic compound binding	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	104	106	PF00675	0.276
CLV_PCSK_KEX2_1	104	106	PF00082	0.355
DEG_Nend_Nbox_1	1	3	PF02207	0.698
DOC_CKS1_1	7	12	PF01111	0.643
DOC_MAPK_MEF2A_6	33	41	PF00069	0.282
DOC_WW_Pin1_4	18	23	PF00397	0.337
DOC_WW_Pin1_4	6	11	PF00397	0.540
LIG_14-3-3_CanoR_1	104	110	PF00244	0.427
LIG_14-3-3_CanoR_1	23	29	PF00244	0.291
LIG_BRCT_BRCA1_1	10	14	PF00533	0.623
LIG_BRCT_BRCA1_1	37	41	PF00533	0.394
LIG_FHA_1	108	114	PF00498	0.427
LIG_FHA_1	81	87	PF00498	0.427
LIG_LIR_Gen_1	35	43	PF02991	0.282
LIG_LIR_Nem_3	11	17	PF02991	0.578
LIG_LIR_Nem_3	35	39	PF02991	0.281
LIG_LIR_Nem_3	74	78	PF02991	0.293
LIG_LIR_Nem_3	97	103	PF02991	0.298
LIG_SH2_CRK	103	107	PF00017	0.282
LIG_SH2_PTP2	36	39	PF00017	0.282
LIG_SH2_PTP2	78	81	PF00017	0.282
LIG_SH2_SRC	78	81	PF00017	0.282
LIG_SH2_STAP1	2	6	PF00017	0.685
LIG_SH2_STAT5	36	39	PF00017	0.282
LIG_SH2_STAT5	78	81	PF00017	0.274
LIG_SH2_STAT5	88	91	PF00017	0.253
LIG_SH3_3	58	64	PF00018	0.394
LIG_SUMO_SIM_anti_2	35	42	PF11976	0.282
LIG_SUMO_SIM_par_1	105	111	PF11976	0.426
MOD_CDK_SPK_2	18	23	PF00069	0.395
MOD_CDK_SPxxK_3	6	13	PF00069	0.569
MOD_CK1_1	108	114	PF00069	0.427
MOD_CK1_1	8	14	PF00069	0.552
MOD_GSK3_1	18	25	PF00069	0.317
MOD_GSK3_1	80	87	PF00069	0.394
MOD_NEK2_1	41	46	PF00069	0.375
MOD_PKA_2	22	28	PF00069	0.286
MOD_Plk_1	67	73	PF00069	0.390
MOD_Plk_1	84	90	PF00069	0.394
MOD_Plk_4	35	41	PF00069	0.321
MOD_Plk_4	84	90	PF00069	0.394
MOD_ProDKin_1	18	24	PF00069	0.224
MOD_ProDKin_1	6	12	PF00069	0.537
TRG_ENDOCYTIC_2	103	106	PF00928	0.282
TRG_ENDOCYTIC_2	36	39	PF00928	0.266
TRG_ER_diArg_1	103	105	PF00400	0.355

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IJ21	Leptomonas seymouri	84%	99%
A0A1X0P5K0	Trypanosomatidae	42%	92%
A0A3Q8IFC3	Leishmania donovani	100%	100%
A0A3R7ML99	Trypanosoma rangeli	49%	91%
A4HN11	Leishmania braziliensis	97%	100%
A4IBN4	Leishmania infantum	100%	100%
C9ZYZ7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	46%	92%
E9B6M3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	98%	100%
V5C325	Trypanosoma cruzi	49%	92%