LEM3 family/CDC50 family protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

LEM3 (ligand-effect modulator 3) family / CDC50 family, putative

Species:

Leishmania major

UniProt:

E9AFG4_LEIMA

TriTrypDb:

LmjF.35.3400 , LMJLV39_350041400 , LMJSD75_350040900

Length:

421

Annotations

LeishMANIAdb annotations

Homologous to animal CDC50 family phospholipid flippase (ATPase) proteins.. This family has expanded in kinetoplastids

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 26
NetGPI	no	yes: 0, no: 26

Cellular components
Term	Name	Level	Count
GO:0005783	endoplasmic reticulum	5	3
GO:0005794	Golgi apparatus	5	3
GO:0005886	plasma membrane	3	3
GO:0016020	membrane	2	27
GO:0043226	organelle	2	3
GO:0043227	membrane-bounded organelle	3	3
GO:0043229	intracellular organelle	3	3
GO:0043231	intracellular membrane-bounded organelle	4	3
GO:0110165	cellular anatomical entity	1	27
GO:0005737	cytoplasm	2	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1
GO:0097014	ciliary plasm	5	1
GO:0099568	cytoplasmic region	3	1

Expansion

Sequence features

E9AFG4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFG4

Function

Biological processes
Term	Name	Level	Count
GO:0033036	macromolecule localization	2	27
GO:0051179	localization	1	27

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	132	136	PF00656	0.253
CLV_C14_Caspase3-7	277	281	PF00656	0.305
CLV_NRD_NRD_1	127	129	PF00675	0.430
CLV_NRD_NRD_1	331	333	PF00675	0.443
CLV_PCSK_KEX2_1	127	129	PF00082	0.430
CLV_PCSK_SKI1_1	107	111	PF00082	0.470
DEG_SCF_FBW7_1	28	35	PF00400	0.596
DOC_CKS1_1	29	34	PF01111	0.498
DOC_CYCLIN_yCln2_LP_2	109	115	PF00134	0.201
DOC_MAPK_gen_1	107	115	PF00069	0.247
DOC_MAPK_gen_1	409	418	PF00069	0.509
DOC_PP1_RVXF_1	240	247	PF00149	0.231
DOC_PP2B_LxvP_1	109	112	PF13499	0.237
DOC_PP4_FxxP_1	259	262	PF00568	0.268
DOC_USP7_MATH_1	147	151	PF00917	0.312
DOC_USP7_MATH_1	245	249	PF00917	0.270
DOC_USP7_MATH_1	32	36	PF00917	0.476
DOC_USP7_MATH_1	56	60	PF00917	0.299
DOC_WW_Pin1_4	150	155	PF00397	0.252
DOC_WW_Pin1_4	261	266	PF00397	0.327
DOC_WW_Pin1_4	28	33	PF00397	0.517
DOC_WW_Pin1_4	85	90	PF00397	0.350
LIG_14-3-3_CanoR_1	142	148	PF00244	0.354
LIG_14-3-3_CanoR_1	162	169	PF00244	0.113
LIG_14-3-3_CanoR_1	266	272	PF00244	0.280
LIG_14-3-3_CanoR_1	310	320	PF00244	0.277
LIG_14-3-3_CanoR_1	324	329	PF00244	0.255
LIG_14-3-3_CanoR_1	377	381	PF00244	0.324
LIG_14-3-3_CanoR_1	55	65	PF00244	0.251
LIG_AP2alpha_1	194	198	PF02296	0.232
LIG_CtBP_PxDLS_1	48	52	PF00389	0.409
LIG_eIF4E_1	183	189	PF01652	0.207
LIG_FHA_1	150	156	PF00498	0.286
LIG_FHA_1	195	201	PF00498	0.247
LIG_FHA_1	245	251	PF00498	0.338
LIG_FHA_1	93	99	PF00498	0.256
LIG_FHA_2	202	208	PF00498	0.314
LIG_FHA_2	312	318	PF00498	0.241
LIG_LIR_Apic_2	251	257	PF02991	0.217
LIG_LIR_Gen_1	13	23	PF02991	0.562
LIG_LIR_Gen_1	179	189	PF02991	0.254
LIG_LIR_Gen_1	223	233	PF02991	0.299
LIG_LIR_Gen_1	398	406	PF02991	0.379
LIG_LIR_Nem_3	13	18	PF02991	0.527
LIG_LIR_Nem_3	174	178	PF02991	0.233
LIG_LIR_Nem_3	179	184	PF02991	0.223
LIG_LIR_Nem_3	196	201	PF02991	0.238
LIG_LIR_Nem_3	223	228	PF02991	0.278
LIG_LIR_Nem_3	398	402	PF02991	0.397
LIG_MYND_1	28	32	PF01753	0.514
LIG_OCRL_FandH_1	398	410	PF00620	0.204
LIG_Pex14_2	194	198	PF04695	0.232
LIG_Pex14_2	298	302	PF04695	0.243
LIG_PTB_Apo_2	192	199	PF02174	0.196
LIG_SH2_CRK	254	258	PF00017	0.257
LIG_SH2_GRB2like	123	126	PF00017	0.225
LIG_SH2_GRB2like	297	300	PF00017	0.256
LIG_SH2_NCK_1	276	280	PF00017	0.178
LIG_SH2_SRC	123	126	PF00017	0.247
LIG_SH2_SRC	183	186	PF00017	0.299
LIG_SH2_STAP1	178	182	PF00017	0.324
LIG_SH2_STAT3	67	70	PF00017	0.198
LIG_SH2_STAT5	114	117	PF00017	0.194
LIG_SH2_STAT5	183	186	PF00017	0.273
LIG_SH2_STAT5	297	300	PF00017	0.273
LIG_SH2_STAT5	347	350	PF00017	0.237
LIG_SH2_STAT5	385	388	PF00017	0.292
LIG_SH3_1	254	260	PF00018	0.297
LIG_SH3_2	285	290	PF14604	0.183
LIG_SH3_3	179	185	PF00018	0.281
LIG_SH3_3	208	214	PF00018	0.282
LIG_SH3_3	254	260	PF00018	0.276
LIG_SH3_3	282	288	PF00018	0.333
LIG_SH3_3	42	48	PF00018	0.338
LIG_SH3_3	6	12	PF00018	0.545
LIG_SUMO_SIM_par_1	47	52	PF11976	0.311
LIG_WRC_WIRS_1	172	177	PF05994	0.198
MOD_CDK_SPK_2	261	266	PF00069	0.148
MOD_CK1_1	150	156	PF00069	0.248
MOD_CK1_1	223	229	PF00069	0.249
MOD_CK1_1	367	373	PF00069	0.245
MOD_CK1_1	392	398	PF00069	0.421
MOD_CK2_1	201	207	PF00069	0.310
MOD_CK2_1	245	251	PF00069	0.325
MOD_CK2_1	311	317	PF00069	0.240
MOD_GlcNHglycan	143	146	PF01048	0.443
MOD_GlcNHglycan	149	152	PF01048	0.431
MOD_GlcNHglycan	222	225	PF01048	0.510
MOD_GlcNHglycan	269	272	PF01048	0.450
MOD_GlcNHglycan	34	37	PF01048	0.349
MOD_GlcNHglycan	391	394	PF01048	0.356
MOD_GlcNHglycan	58	61	PF01048	0.524
MOD_GSK3_1	127	134	PF00069	0.216
MOD_GSK3_1	149	156	PF00069	0.229
MOD_GSK3_1	28	35	PF00069	0.569
MOD_GSK3_1	286	293	PF00069	0.284
MOD_GSK3_1	356	363	PF00069	0.206
MOD_GSK3_1	92	99	PF00069	0.230
MOD_N-GLC_1	194	199	PF02516	0.414
MOD_N-GLC_1	220	225	PF02516	0.450
MOD_N-GLC_1	56	61	PF02516	0.524
MOD_N-GLC_1	77	82	PF02516	0.515
MOD_NEK2_1	129	134	PF00069	0.264
MOD_NEK2_1	171	176	PF00069	0.276
MOD_NEK2_1	194	199	PF00069	0.218
MOD_NEK2_1	384	389	PF00069	0.285
MOD_NEK2_1	49	54	PF00069	0.326
MOD_PKA_1	127	133	PF00069	0.268
MOD_PKA_2	10	16	PF00069	0.593
MOD_PKA_2	127	133	PF00069	0.263
MOD_PKA_2	141	147	PF00069	0.275
MOD_PKA_2	161	167	PF00069	0.113
MOD_PKA_2	201	207	PF00069	0.270
MOD_PKA_2	226	232	PF00069	0.298
MOD_PKA_2	376	382	PF00069	0.329
MOD_Plk_1	194	200	PF00069	0.214
MOD_Plk_1	56	62	PF00069	0.273
MOD_Plk_4	10	16	PF00069	0.603
MOD_Plk_4	171	177	PF00069	0.315
MOD_Plk_4	343	349	PF00069	0.338
MOD_Plk_4	369	375	PF00069	0.231
MOD_Plk_4	376	382	PF00069	0.235
MOD_Plk_4	395	401	PF00069	0.344
MOD_Plk_4	49	55	PF00069	0.338
MOD_ProDKin_1	150	156	PF00069	0.252
MOD_ProDKin_1	261	267	PF00069	0.327
MOD_ProDKin_1	28	34	PF00069	0.515
MOD_ProDKin_1	85	91	PF00069	0.350
TRG_DiLeu_BaLyEn_6	214	219	PF01217	0.257
TRG_ENDOCYTIC_2	116	119	PF00928	0.229
TRG_ENDOCYTIC_2	178	181	PF00928	0.225
TRG_ENDOCYTIC_2	335	338	PF00928	0.238
TRG_ENDOCYTIC_2	385	388	PF00928	0.318
TRG_ER_diArg_1	127	129	PF00400	0.227
TRG_Pf-PMV_PEXEL_1	336	340	PF00026	0.524

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I0U8	Leptomonas seymouri	34%	67%
A0A0N1PBG2	Leptomonas seymouri	70%	100%
A0A0N1PDQ9	Leptomonas seymouri	33%	100%
A0A0S4IMK4	Bodo saltans	43%	100%
A0A0S4J4T1	Bodo saltans	33%	100%
A0A0S4JB98	Bodo saltans	34%	100%
A0A0S4JJY0	Bodo saltans	34%	100%
A0A0S4JQR9	Bodo saltans	31%	80%
A0A1X0NHQ5	Trypanosomatidae	32%	88%
A0A1X0NV71	Trypanosomatidae	29%	100%
A0A1X0P5L3	Trypanosomatidae	50%	100%
A0A3Q8IBY5	Leishmania donovani	35%	71%
A0A3R7LIN7	Trypanosoma rangeli	34%	98%
A0A3R7MGX3	Trypanosoma rangeli	33%	100%
A0A3S7X551	Leishmania donovani	31%	100%
A0A3S7X9N4	Leishmania donovani	97%	100%
A0A422NR22	Trypanosoma rangeli	49%	100%
A4H5T1	Leishmania braziliensis	33%	69%
A4HK31	Leishmania braziliensis	29%	100%
A4HN07	Leishmania braziliensis	86%	100%
A4HU24	Leishmania infantum	35%	71%
A4I7N1	Leishmania infantum	31%	100%
A4IBN0	Leishmania infantum	96%	100%
C9ZZ02	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	40%	100%
D0A9K3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	34%	90%
D0A9R9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	29%	100%
E9AMV4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	33%	71%
E9B2H4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	30%	100%
E9B6L9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	100%
H2L0H3	Caenorhabditis elegans	27%	100%
P25656	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	27%	100%
P42838	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	26%	100%
P53740	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	27%	100%
Q17QL5	Bos taurus	30%	100%
Q1MTQ5	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	29%	100%
Q2T9P5	Bos taurus	25%	100%
Q3MIR4	Homo sapiens	26%	100%
Q4Q5N2	Leishmania major	31%	100%
Q4QHS2	Leishmania major	35%	71%
Q5F362	Gallus gallus	29%	100%
Q5R6C0	Pongo abelii	29%	100%
Q67YS6	Arabidopsis thaliana	30%	100%
Q6AY41	Rattus norvegicus	30%	100%
Q8BHG3	Mus musculus	28%	100%
Q8L8W0	Arabidopsis thaliana	32%	100%
Q8VEK0	Mus musculus	29%	100%
Q95JK4	Macaca fascicularis	25%	100%
Q96WW4	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	27%	100%
Q9D4D7	Mus musculus	25%	100%
Q9LTW0	Arabidopsis thaliana	30%	100%
Q9NV96	Homo sapiens	29%	100%
Q9SA35	Arabidopsis thaliana	31%	100%
Q9SLK2	Arabidopsis thaliana	32%	100%
V5AY40	Trypanosoma cruzi	35%	100%
V5B6A6	Trypanosoma cruzi	33%	100%
V5BY23	Trypanosoma cruzi	49%	100%