Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

poly A polymerase regulatory subunit, putative

Species:

Leishmania major

UniProt:

E9AFG2_LEIMA

TriTrypDb:

LmjF.35.3380 , LMJLV39_350041200 , LMJSD75_350040700

Length:

547

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

E9AFG2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFG2

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006370	7-methylguanosine mRNA capping	8	12
GO:0006396	RNA processing	6	12
GO:0006397	mRNA processing	7	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009452	7-methylguanosine RNA capping	8	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0016071	mRNA metabolic process	6	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0036260	RNA capping	7	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	11
GO:0003746	translation elongation factor activity	4	11
GO:0003824	catalytic activity	1	12
GO:0004483	mRNA (nucleoside-2'-O-)-methyltransferase activity	6	12
GO:0005488	binding	1	11
GO:0008135	translation factor activity, RNA binding	3	11
GO:0008168	methyltransferase activity	4	12
GO:0008171	O-methyltransferase activity	5	12
GO:0008173	RNA methyltransferase activity	4	12
GO:0008174	mRNA methyltransferase activity	5	12
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	12
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12
GO:0045182	translation regulator activity	1	11
GO:0090079	translation regulator activity, nucleic acid binding	2	11
GO:0097159	organic cyclic compound binding	2	11
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901363	heterocyclic compound binding	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	164	166	PF00675	0.375
CLV_NRD_NRD_1	409	411	PF00675	0.511
CLV_NRD_NRD_1	434	436	PF00675	0.443
CLV_NRD_NRD_1	7	9	PF00675	0.658
CLV_NRD_NRD_1	80	82	PF00675	0.344
CLV_PCSK_KEX2_1	164	166	PF00082	0.375
CLV_PCSK_KEX2_1	433	435	PF00082	0.497
CLV_PCSK_KEX2_1	7	9	PF00082	0.586
CLV_PCSK_PC1ET2_1	7	9	PF00082	0.586
CLV_PCSK_SKI1_1	137	141	PF00082	0.344
CLV_PCSK_SKI1_1	164	168	PF00082	0.501
CLV_PCSK_SKI1_1	238	242	PF00082	0.382
CLV_PCSK_SKI1_1	37	41	PF00082	0.654
CLV_PCSK_SKI1_1	402	406	PF00082	0.551
CLV_PCSK_SKI1_1	411	415	PF00082	0.419
CLV_PCSK_SKI1_1	435	439	PF00082	0.484
CLV_PCSK_SKI1_1	70	74	PF00082	0.432
CLV_PCSK_SKI1_1	81	85	PF00082	0.226
CLV_Separin_Metazoa	412	416	PF03568	0.521
DEG_SPOP_SBC_1	490	494	PF00917	0.760
DOC_CYCLIN_RxL_1	235	245	PF00134	0.369
DOC_CYCLIN_RxL_1	77	87	PF00134	0.363
DOC_CYCLIN_yCln2_LP_2	50	56	PF00134	0.425
DOC_MAPK_DCC_7	165	175	PF00069	0.462
DOC_MAPK_gen_1	236	243	PF00069	0.389
DOC_MAPK_gen_1	410	416	PF00069	0.537
DOC_MAPK_gen_1	433	440	PF00069	0.465
DOC_MAPK_MEF2A_6	236	243	PF00069	0.440
DOC_MAPK_MEF2A_6	316	323	PF00069	0.417
DOC_MAPK_NFAT4_5	316	324	PF00069	0.438
DOC_PP1_RVXF_1	236	243	PF00149	0.388
DOC_PP2B_LxvP_1	220	223	PF13499	0.463
DOC_PP2B_LxvP_1	334	337	PF13499	0.351
DOC_PP4_FxxP_1	283	286	PF00568	0.390
DOC_PP4_FxxP_1	396	399	PF00568	0.429
DOC_USP7_MATH_1	181	185	PF00917	0.436
DOC_USP7_MATH_1	286	290	PF00917	0.512
DOC_USP7_MATH_1	372	376	PF00917	0.373
DOC_USP7_MATH_1	443	447	PF00917	0.613
DOC_USP7_MATH_1	490	494	PF00917	0.644
DOC_USP7_MATH_1	64	68	PF00917	0.475
DOC_USP7_UBL2_3	445	449	PF12436	0.629
LIG_14-3-3_CanoR_1	124	128	PF00244	0.364
LIG_14-3-3_CanoR_1	164	173	PF00244	0.473
LIG_14-3-3_CanoR_1	284	294	PF00244	0.470
LIG_14-3-3_CanoR_1	326	336	PF00244	0.386
LIG_14-3-3_CanoR_1	518	523	PF00244	0.515
LIG_Actin_WH2_2	387	404	PF00022	0.452
LIG_BIR_III_4	457	461	PF00653	0.648
LIG_BRCT_BRCA1_1	290	294	PF00533	0.627
LIG_BRCT_BRCA1_1	31	35	PF00533	0.587
LIG_BRCT_BRCA1_1	48	52	PF00533	0.543
LIG_BRCT_BRCA1_2	31	37	PF00533	0.483
LIG_CaM_IQ_9	182	197	PF13499	0.615
LIG_FHA_1	124	130	PF00498	0.332
LIG_FHA_1	165	171	PF00498	0.509
LIG_FHA_1	200	206	PF00498	0.485
LIG_FHA_1	207	213	PF00498	0.489
LIG_FHA_1	269	275	PF00498	0.472
LIG_FHA_1	301	307	PF00498	0.586
LIG_FHA_1	38	44	PF00498	0.600
LIG_FHA_1	404	410	PF00498	0.550
LIG_FHA_1	419	425	PF00498	0.295
LIG_FHA_1	493	499	PF00498	0.665
LIG_FHA_1	504	510	PF00498	0.483
LIG_FHA_2	326	332	PF00498	0.403
LIG_FHA_2	425	431	PF00498	0.483
LIG_GBD_Chelix_1	416	424	PF00786	0.549
LIG_HCF-1_HBM_1	378	381	PF13415	0.420
LIG_Integrin_RGD_1	316	318	PF01839	0.462
LIG_IRF3_LxIS_1	239	244	PF10401	0.475
LIG_LIR_Apic_2	42	47	PF02991	0.476
LIG_LIR_Apic_2	92	97	PF02991	0.396
LIG_LIR_Gen_1	126	135	PF02991	0.328
LIG_LIR_Gen_1	18	28	PF02991	0.696
LIG_LIR_Gen_1	268	274	PF02991	0.380
LIG_LIR_Gen_1	32	43	PF02991	0.652
LIG_LIR_Gen_1	392	401	PF02991	0.443
LIG_LIR_Gen_1	98	109	PF02991	0.364
LIG_LIR_LC3C_4	331	336	PF02991	0.381
LIG_LIR_Nem_3	126	130	PF02991	0.328
LIG_LIR_Nem_3	18	23	PF02991	0.689
LIG_LIR_Nem_3	268	273	PF02991	0.385
LIG_LIR_Nem_3	32	38	PF02991	0.700
LIG_LIR_Nem_3	351	357	PF02991	0.379
LIG_LIR_Nem_3	392	396	PF02991	0.427
LIG_LIR_Nem_3	49	55	PF02991	0.438
LIG_LIR_Nem_3	98	104	PF02991	0.341
LIG_PCNA_PIPBox_1	13	22	PF02747	0.659
LIG_PCNA_yPIPBox_3	7	20	PF02747	0.667
LIG_PTB_Apo_2	14	21	PF02174	0.657
LIG_PTB_Apo_2	147	154	PF02174	0.328
LIG_PTB_Phospho_1	14	20	PF10480	0.655
LIG_SH2_CRK	101	105	PF00017	0.371
LIG_SH2_CRK	177	181	PF00017	0.333
LIG_SH2_CRK	20	24	PF00017	0.612
LIG_SH2_CRK	44	48	PF00017	0.474
LIG_SH2_GRB2like	177	180	PF00017	0.418
LIG_SH2_NCK_1	381	385	PF00017	0.398
LIG_SH2_SRC	177	180	PF00017	0.465
LIG_SH2_STAP1	381	385	PF00017	0.398
LIG_SH2_STAT5	287	290	PF00017	0.468
LIG_SH2_STAT5	314	317	PF00017	0.392
LIG_SH2_STAT5	393	396	PF00017	0.403
LIG_SH2_STAT5	5	8	PF00017	0.723
LIG_SH2_STAT5	94	97	PF00017	0.378
LIG_SH3_2	61	66	PF14604	0.544
LIG_SH3_3	136	142	PF00018	0.410
LIG_SH3_3	248	254	PF00018	0.481
LIG_SH3_3	494	500	PF00018	0.680
LIG_SH3_3	58	64	PF00018	0.542
LIG_SUMO_SIM_par_1	302	309	PF11976	0.382
LIG_SUMO_SIM_par_1	52	59	PF11976	0.477
LIG_TYR_ITIM	99	104	PF00017	0.385
LIG_WRC_WIRS_1	25	30	PF05994	0.477
MOD_CK1_1	21	27	PF00069	0.629
MOD_CK1_1	289	295	PF00069	0.571
MOD_CK1_1	471	477	PF00069	0.725
MOD_CK1_1	480	486	PF00069	0.750
MOD_CK1_1	521	527	PF00069	0.598
MOD_CK1_1	533	539	PF00069	0.640
MOD_CK2_1	325	331	PF00069	0.390
MOD_CK2_1	357	363	PF00069	0.478
MOD_CK2_1	498	504	PF00069	0.575
MOD_GlcNHglycan	2	5	PF01048	0.719
MOD_GlcNHglycan	20	23	PF01048	0.663
MOD_GlcNHglycan	247	250	PF01048	0.412
MOD_GlcNHglycan	31	34	PF01048	0.494
MOD_GlcNHglycan	470	473	PF01048	0.727
MOD_GlcNHglycan	482	485	PF01048	0.582
MOD_GlcNHglycan	535	538	PF01048	0.576
MOD_GlcNHglycan	73	76	PF01048	0.449
MOD_GSK3_1	119	126	PF00069	0.415
MOD_GSK3_1	23	30	PF00069	0.657
MOD_GSK3_1	241	248	PF00069	0.372
MOD_GSK3_1	285	292	PF00069	0.508
MOD_GSK3_1	322	329	PF00069	0.397
MOD_GSK3_1	33	40	PF00069	0.598
MOD_N-GLC_1	154	159	PF02516	0.374
MOD_N-GLC_1	357	362	PF02516	0.476
MOD_N-GLC_1	480	485	PF02516	0.721
MOD_N-GLC_1	518	523	PF02516	0.522
MOD_NEK2_1	23	28	PF00069	0.682
MOD_NEK2_1	356	361	PF00069	0.480
MOD_NEK2_1	538	543	PF00069	0.596
MOD_PIKK_1	443	449	PF00454	0.603
MOD_PK_1	247	253	PF00069	0.446
MOD_PK_1	518	524	PF00069	0.443
MOD_PKA_1	164	170	PF00069	0.466
MOD_PKA_2	123	129	PF00069	0.363
MOD_PKA_2	164	170	PF00069	0.438
MOD_PKA_2	206	212	PF00069	0.517
MOD_PKA_2	300	306	PF00069	0.633
MOD_PKA_2	325	331	PF00069	0.390
MOD_PKA_2	511	517	PF00069	0.564
MOD_Plk_1	154	160	PF00069	0.374
MOD_Plk_1	276	282	PF00069	0.449
MOD_Plk_1	357	363	PF00069	0.478
MOD_Plk_1	518	524	PF00069	0.514
MOD_Plk_1	97	103	PF00069	0.364
MOD_Plk_4	276	282	PF00069	0.449
MOD_Plk_4	300	306	PF00069	0.489
MOD_Plk_4	389	395	PF00069	0.403
MOD_Plk_4	89	95	PF00069	0.377
MOD_SUMO_rev_2	183	192	PF00179	0.595
MOD_SUMO_rev_2	462	472	PF00179	0.677
MOD_SUMO_rev_2	67	75	PF00179	0.363
TRG_DiLeu_BaEn_1	386	391	PF01217	0.439
TRG_DiLeu_BaLyEn_6	236	241	PF01217	0.463
TRG_ENDOCYTIC_2	101	104	PF00928	0.344
TRG_ENDOCYTIC_2	177	180	PF00928	0.365
TRG_ENDOCYTIC_2	20	23	PF00928	0.690
TRG_ENDOCYTIC_2	393	396	PF00928	0.397
TRG_ER_diArg_1	146	149	PF00400	0.464
TRG_ER_diArg_1	164	166	PF00400	0.277
TRG_ER_diArg_1	432	435	PF00400	0.448
TRG_ER_diArg_1	65	68	PF00400	0.363
TRG_ER_FFAT_2	14	24	PF00635	0.686
TRG_NES_CRM1_1	13	29	PF08389	0.460
TRG_NLS_MonoCore_2	6	11	PF00514	0.647
TRG_NLS_MonoExtC_3	7	12	PF00514	0.591
TRG_NLS_MonoExtN_4	7	13	PF00514	0.637
TRG_Pf-PMV_PEXEL_1	164	168	PF00026	0.437

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PB31	Leptomonas seymouri	74%	100%
A0A0S4IHM6	Bodo saltans	52%	100%
A0A0S4JS06	Bodo saltans	46%	100%
A0A1X0P597	Trypanosomatidae	63%	100%
A0A3S7X9K6	Leishmania donovani	97%	100%
A0A422NR92	Trypanosoma rangeli	58%	100%
A4HN05	Leishmania braziliensis	87%	100%
A4IBM8	Leishmania infantum	97%	100%
C9ZZ04	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	59%	100%
E9B6L7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	100%