Probable eukaryotic initiation factor 4A

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ATP-dependent RNA helicase, putative

Species:

Leishmania major

UniProt:

E9AFD4_LEIMA

TriTrypDb:

LmjF.35.3100 , LMJLV39_350038300 , LMJSD75_350037700

Length:

923

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 6
Forrest at al. (procyclic)	no	yes: 6
Silverman et al.	yes	yes: 2
Pissara et al.	yes	yes: 18

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	yes	yes: 8

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 12

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	3
GO:0043226	organelle	2	3
GO:0043227	membrane-bounded organelle	3	3
GO:0043229	intracellular organelle	3	3
GO:0043231	intracellular membrane-bounded organelle	4	3
GO:0110165	cellular anatomical entity	1	3
GO:0005737	cytoplasm	2	1
GO:0010494	cytoplasmic stress granule	5	1
GO:0034399	nuclear periphery	2	1
GO:0035770	ribonucleoprotein granule	3	1
GO:0036464	cytoplasmic ribonucleoprotein granule	4	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0097165	nuclear stress granule	4	1
GO:0099080	supramolecular complex	2	1

Expansion

Sequence features

E9AFD4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFD4

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	10
GO:0003676	nucleic acid binding	3	10
GO:0003723	RNA binding	4	3
GO:0003724	RNA helicase activity	3	10
GO:0003743	translation initiation factor activity	4	10
GO:0003824	catalytic activity	1	10
GO:0004386	helicase activity	2	10
GO:0005488	binding	1	10
GO:0005524	ATP binding	5	10
GO:0008135	translation factor activity, RNA binding	3	10
GO:0008186	ATP-dependent activity, acting on RNA	2	10
GO:0016787	hydrolase activity	2	10
GO:0017076	purine nucleotide binding	4	10
GO:0030554	adenyl nucleotide binding	5	10
GO:0032553	ribonucleotide binding	3	10
GO:0032555	purine ribonucleotide binding	4	10
GO:0032559	adenyl ribonucleotide binding	5	10
GO:0035639	purine ribonucleoside triphosphate binding	4	10
GO:0036094	small molecule binding	2	10
GO:0043167	ion binding	2	10
GO:0043168	anion binding	3	10
GO:0045182	translation regulator activity	1	10
GO:0090079	translation regulator activity, nucleic acid binding	2	10
GO:0097159	organic cyclic compound binding	2	10
GO:0097367	carbohydrate derivative binding	2	10
GO:0140098	catalytic activity, acting on RNA	3	10
GO:0140640	catalytic activity, acting on a nucleic acid	2	10
GO:0140657	ATP-dependent activity	1	10
GO:1901265	nucleoside phosphate binding	3	10
GO:1901363	heterocyclic compound binding	2	10

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	238	242	PF00656	0.649
CLV_C14_Caspase3-7	899	903	PF00656	0.759
CLV_NRD_NRD_1	175	177	PF00675	0.734
CLV_NRD_NRD_1	219	221	PF00675	0.729
CLV_NRD_NRD_1	234	236	PF00675	0.648
CLV_NRD_NRD_1	261	263	PF00675	0.730
CLV_NRD_NRD_1	274	276	PF00675	0.655
CLV_NRD_NRD_1	309	311	PF00675	0.742
CLV_NRD_NRD_1	316	318	PF00675	0.705
CLV_NRD_NRD_1	338	340	PF00675	0.802
CLV_NRD_NRD_1	418	420	PF00675	0.688
CLV_NRD_NRD_1	42	44	PF00675	0.753
CLV_NRD_NRD_1	427	429	PF00675	0.551
CLV_NRD_NRD_1	542	544	PF00675	0.444
CLV_NRD_NRD_1	566	568	PF00675	0.507
CLV_NRD_NRD_1	675	677	PF00675	0.533
CLV_NRD_NRD_1	737	739	PF00675	0.396
CLV_NRD_NRD_1	753	755	PF00675	0.319
CLV_NRD_NRD_1	828	830	PF00675	0.539
CLV_NRD_NRD_1	872	874	PF00675	0.749
CLV_NRD_NRD_1	913	915	PF00675	0.772
CLV_PCSK_FUR_1	314	318	PF00082	0.717
CLV_PCSK_FUR_1	40	44	PF00082	0.770
CLV_PCSK_KEX2_1	175	177	PF00082	0.734
CLV_PCSK_KEX2_1	219	221	PF00082	0.729
CLV_PCSK_KEX2_1	233	235	PF00082	0.662
CLV_PCSK_KEX2_1	261	263	PF00082	0.730
CLV_PCSK_KEX2_1	274	276	PF00082	0.655
CLV_PCSK_KEX2_1	309	311	PF00082	0.742
CLV_PCSK_KEX2_1	316	318	PF00082	0.705
CLV_PCSK_KEX2_1	338	340	PF00082	0.802
CLV_PCSK_KEX2_1	418	420	PF00082	0.720
CLV_PCSK_KEX2_1	42	44	PF00082	0.753
CLV_PCSK_KEX2_1	427	429	PF00082	0.581
CLV_PCSK_KEX2_1	671	673	PF00082	0.502
CLV_PCSK_KEX2_1	675	677	PF00082	0.506
CLV_PCSK_KEX2_1	737	739	PF00082	0.396
CLV_PCSK_KEX2_1	753	755	PF00082	0.319
CLV_PCSK_KEX2_1	828	830	PF00082	0.581
CLV_PCSK_KEX2_1	871	873	PF00082	0.745
CLV_PCSK_KEX2_1	913	915	PF00082	0.772
CLV_PCSK_PC1ET2_1	671	673	PF00082	0.502
CLV_PCSK_SKI1_1	418	422	PF00082	0.660
CLV_PCSK_SKI1_1	464	468	PF00082	0.601
CLV_PCSK_SKI1_1	567	571	PF00082	0.504
CLV_PCSK_SKI1_1	603	607	PF00082	0.396
CLV_PCSK_SKI1_1	691	695	PF00082	0.569
CLV_PCSK_SKI1_1	829	833	PF00082	0.540
DEG_Nend_UBRbox_2	1	3	PF02207	0.737
DEG_SPOP_SBC_1	686	690	PF00917	0.707
DOC_ANK_TNKS_1	883	890	PF00023	0.719
DOC_CYCLIN_RxL_1	614	625	PF00134	0.596
DOC_CYCLIN_yCln2_LP_2	476	482	PF00134	0.522
DOC_MAPK_DCC_7	766	774	PF00069	0.630
DOC_MAPK_gen_1	541	550	PF00069	0.596
DOC_MAPK_gen_1	675	683	PF00069	0.500
DOC_MAPK_gen_1	766	774	PF00069	0.661
DOC_MAPK_gen_1	825	834	PF00069	0.583
DOC_MAPK_MEF2A_6	498	505	PF00069	0.610
DOC_MAPK_MEF2A_6	543	552	PF00069	0.596
DOC_MAPK_MEF2A_6	825	834	PF00069	0.542
DOC_PP1_RVXF_1	565	572	PF00149	0.596
DOC_PP2B_LxvP_1	783	786	PF13499	0.732
DOC_PP4_FxxP_1	168	171	PF00568	0.706
DOC_PP4_FxxP_1	524	527	PF00568	0.596
DOC_PP4_FxxP_1	794	797	PF00568	0.596
DOC_USP7_MATH_1	111	115	PF00917	0.732
DOC_USP7_MATH_1	121	125	PF00917	0.704
DOC_USP7_MATH_1	361	365	PF00917	0.773
DOC_USP7_MATH_1	46	50	PF00917	0.765
DOC_WW_Pin1_4	107	112	PF00397	0.730
DOC_WW_Pin1_4	142	147	PF00397	0.706
DOC_WW_Pin1_4	362	367	PF00397	0.769
DOC_WW_Pin1_4	404	409	PF00397	0.778
DOC_WW_Pin1_4	491	496	PF00397	0.631
DOC_WW_Pin1_4	535	540	PF00397	0.596
DOC_WW_Pin1_4	767	772	PF00397	0.596
LIG_14-3-3_CanoR_1	541	547	PF00244	0.609
LIG_14-3-3_CanoR_1	567	572	PF00244	0.704
LIG_14-3-3_CanoR_1	643	650	PF00244	0.639
LIG_14-3-3_CanoR_1	653	657	PF00244	0.633
LIG_14-3-3_CanoR_1	685	693	PF00244	0.709
LIG_APCC_ABBA_1	560	565	PF00400	0.681
LIG_APCC_ABBA_1	722	727	PF00400	0.681
LIG_BIR_III_4	115	119	PF00653	0.718
LIG_BRCT_BRCA1_1	658	662	PF00533	0.681
LIG_Clathr_ClatBox_1	723	727	PF01394	0.681
LIG_CtBP_PxDLS_1	458	462	PF00389	0.559
LIG_EH1_1	715	723	PF00400	0.596
LIG_FHA_1	553	559	PF00498	0.681
LIG_FHA_1	688	694	PF00498	0.698
LIG_FHA_1	768	774	PF00498	0.596
LIG_FHA_1	802	808	PF00498	0.596
LIG_FHA_2	25	31	PF00498	0.744
LIG_FHA_2	291	297	PF00498	0.728
LIG_FHA_2	647	653	PF00498	0.596
LIG_FHA_2	97	103	PF00498	0.740
LIG_LIR_Apic_2	165	171	PF02991	0.710
LIG_LIR_Apic_2	323	329	PF02991	0.734
LIG_LIR_Apic_2	521	527	PF02991	0.681
LIG_LIR_Gen_1	452	462	PF02991	0.712
LIG_LIR_Gen_1	470	480	PF02991	0.656
LIG_LIR_Gen_1	574	581	PF02991	0.632
LIG_LIR_Gen_1	608	618	PF02991	0.669
LIG_LIR_Gen_1	642	650	PF02991	0.596
LIG_LIR_Nem_3	302	308	PF02991	0.705
LIG_LIR_Nem_3	340	344	PF02991	0.752
LIG_LIR_Nem_3	452	457	PF02991	0.740
LIG_LIR_Nem_3	470	475	PF02991	0.540
LIG_LIR_Nem_3	573	579	PF02991	0.653
LIG_LIR_Nem_3	608	613	PF02991	0.677
LIG_Pex14_2	658	662	PF04695	0.596
LIG_PTAP_UEV_1	904	909	PF05743	0.745
LIG_PTB_Apo_2	604	611	PF02174	0.596
LIG_PTB_Phospho_1	604	610	PF10480	0.596
LIG_SH2_CRK	326	330	PF00017	0.736
LIG_SH2_CRK	454	458	PF00017	0.605
LIG_SH2_CRK	490	494	PF00017	0.659
LIG_SH2_CRK	499	503	PF00017	0.518
LIG_SH2_CRK	531	535	PF00017	0.647
LIG_SH2_CRK	577	581	PF00017	0.599
LIG_SH2_GRB2like	3	6	PF00017	0.705
LIG_SH2_NCK_1	378	382	PF00017	0.651
LIG_SH2_NCK_1	454	458	PF00017	0.602
LIG_SH2_NCK_1	577	581	PF00017	0.596
LIG_SH2_SRC	237	240	PF00017	0.694
LIG_SH2_SRC	300	303	PF00017	0.716
LIG_SH2_SRC	378	381	PF00017	0.658
LIG_SH2_STAP1	247	251	PF00017	0.738
LIG_SH2_STAP1	577	581	PF00017	0.596
LIG_SH2_STAP1	803	807	PF00017	0.596
LIG_SH2_STAT5	3	6	PF00017	0.737
LIG_SH2_STAT5	610	613	PF00017	0.681
LIG_SH2_STAT5	644	647	PF00017	0.596
LIG_SH2_STAT5	673	676	PF00017	0.503
LIG_SH2_STAT5	679	682	PF00017	0.453
LIG_SH2_STAT5	803	806	PF00017	0.681
LIG_SH2_STAT5	837	840	PF00017	0.551
LIG_SH3_3	130	136	PF00018	0.658
LIG_SH3_3	148	154	PF00018	0.715
LIG_SH3_3	402	408	PF00018	0.770
LIG_SH3_3	548	554	PF00018	0.725
LIG_SH3_3	593	599	PF00018	0.681
LIG_SH3_3	902	908	PF00018	0.745
LIG_Sin3_3	473	480	PF02671	0.536
LIG_SUMO_SIM_par_1	148	155	PF11976	0.658
LIG_SUMO_SIM_par_1	501	506	PF11976	0.573
LIG_SUMO_SIM_par_1	770	777	PF11976	0.596
LIG_TRAF2_1	224	227	PF00917	0.734
LIG_TRAF2_1	350	353	PF00917	0.705
LIG_TRAF2_1	374	377	PF00917	0.708
LIG_TYR_ITIM	529	534	PF00017	0.596
LIG_TYR_ITIM	575	580	PF00017	0.596
LIG_UBA3_1	666	671	PF00899	0.491
LIG_WRC_WIRS_1	25	30	PF05994	0.742
LIG_WRC_WIRS_1	341	346	PF05994	0.749
LIG_WRC_WIRS_1	610	615	PF05994	0.596
MOD_CDC14_SPxK_1	538	541	PF00782	0.596
MOD_CDK_SPxK_1	535	541	PF00069	0.596
MOD_CDK_SPxxK_3	491	498	PF00069	0.554
MOD_CK1_1	124	130	PF00069	0.711
MOD_CK1_1	447	453	PF00069	0.622
MOD_CK1_1	518	524	PF00069	0.720
MOD_CK1_1	54	60	PF00069	0.729
MOD_CK1_1	642	648	PF00069	0.596
MOD_CK1_1	745	751	PF00069	0.596
MOD_CK1_1	903	909	PF00069	0.718
MOD_CK2_1	149	155	PF00069	0.719
MOD_CK2_1	290	296	PF00069	0.730
MOD_CK2_1	7	13	PF00069	0.745
MOD_CK2_1	96	102	PF00069	0.741
MOD_Cter_Amidation	217	220	PF01082	0.734
MOD_Cter_Amidation	272	275	PF01082	0.741
MOD_Cter_Amidation	336	339	PF01082	0.758
MOD_Cter_Amidation	40	43	PF01082	0.770
MOD_Cter_Amidation	869	872	PF01082	0.727
MOD_Cter_Amidation	911	914	PF01082	0.772
MOD_GlcNHglycan	133	136	PF01048	0.714
MOD_GlcNHglycan	147	150	PF01048	0.642
MOD_GlcNHglycan	200	203	PF01048	0.747
MOD_GlcNHglycan	252	255	PF01048	0.732
MOD_GlcNHglycan	352	356	PF01048	0.758
MOD_GlcNHglycan	392	395	PF01048	0.719
MOD_GlcNHglycan	414	417	PF01048	0.735
MOD_GlcNHglycan	446	449	PF01048	0.638
MOD_GlcNHglycan	505	508	PF01048	0.417
MOD_GlcNHglycan	517	520	PF01048	0.437
MOD_GlcNHglycan	55	59	PF01048	0.759
MOD_GlcNHglycan	613	616	PF01048	0.396
MOD_GlcNHglycan	658	661	PF01048	0.481
MOD_GlcNHglycan	80	83	PF01048	0.680
MOD_GlcNHglycan	884	887	PF01048	0.766
MOD_GlcNHglycan	905	908	PF01048	0.739
MOD_GSK3_1	107	114	PF00069	0.684
MOD_GSK3_1	121	128	PF00069	0.705
MOD_GSK3_1	145	152	PF00069	0.720
MOD_GSK3_1	198	205	PF00069	0.641
MOD_GSK3_1	362	369	PF00069	0.743
MOD_GSK3_1	642	649	PF00069	0.639
MOD_GSK3_1	652	659	PF00069	0.633
MOD_GSK3_1	687	694	PF00069	0.700
MOD_GSK3_1	773	780	PF00069	0.681
MOD_GSK3_1	896	903	PF00069	0.762
MOD_GSK3_1	96	103	PF00069	0.732
MOD_N-GLC_1	107	112	PF02516	0.716
MOD_N-GLC_1	691	696	PF02516	0.688
MOD_NEK2_1	351	356	PF00069	0.781
MOD_NEK2_1	611	616	PF00069	0.596
MOD_NEK2_1	639	644	PF00069	0.609
MOD_NEK2_1	656	661	PF00069	0.599
MOD_NEK2_1	725	730	PF00069	0.596
MOD_PIKK_1	691	697	PF00454	0.681
MOD_PKA_1	418	424	PF00069	0.661
MOD_PKA_1	567	573	PF00069	0.704
MOD_PKA_1	7	13	PF00069	0.745
MOD_PKA_2	100	106	PF00069	0.672
MOD_PKA_2	418	424	PF00069	0.661
MOD_PKA_2	444	450	PF00069	0.725
MOD_PKA_2	542	548	PF00069	0.596
MOD_PKA_2	642	648	PF00069	0.639
MOD_PKA_2	652	658	PF00069	0.633
MOD_PKA_2	903	909	PF00069	0.746
MOD_PKA_2	91	97	PF00069	0.748
MOD_Plk_2-3	24	30	PF00069	0.744
MOD_Plk_2-3	646	652	PF00069	0.596
MOD_Plk_4	652	658	PF00069	0.681
MOD_ProDKin_1	107	113	PF00069	0.732
MOD_ProDKin_1	142	148	PF00069	0.706
MOD_ProDKin_1	362	368	PF00069	0.763
MOD_ProDKin_1	404	410	PF00069	0.778
MOD_ProDKin_1	491	497	PF00069	0.540
MOD_ProDKin_1	535	541	PF00069	0.596
MOD_ProDKin_1	767	773	PF00069	0.596
MOD_SUMO_rev_2	2	10	PF00179	0.741
MOD_SUMO_rev_2	432	437	PF00179	0.589
MOD_SUMO_rev_2	460	469	PF00179	0.556
TRG_DiLeu_BaEn_1	591	596	PF01217	0.596
TRG_DiLeu_BaEn_1	857	862	PF01217	0.595
TRG_DiLeu_BaLyEn_6	705	710	PF01217	0.596
TRG_ENDOCYTIC_2	454	457	PF00928	0.725
TRG_ENDOCYTIC_2	531	534	PF00928	0.647
TRG_ENDOCYTIC_2	577	580	PF00928	0.599
TRG_ENDOCYTIC_2	610	613	PF00928	0.669
TRG_ENDOCYTIC_2	644	647	PF00928	0.596
TRG_ENDOCYTIC_2	678	681	PF00928	0.522
TRG_ER_diArg_1	219	221	PF00400	0.729
TRG_ER_diArg_1	232	235	PF00400	0.669
TRG_ER_diArg_1	260	262	PF00400	0.728
TRG_ER_diArg_1	274	276	PF00400	0.648
TRG_ER_diArg_1	308	310	PF00400	0.740
TRG_ER_diArg_1	315	317	PF00400	0.703
TRG_ER_diArg_1	329	332	PF00400	0.681
TRG_ER_diArg_1	40	43	PF00400	0.770
TRG_ER_diArg_1	417	419	PF00400	0.752
TRG_ER_diArg_1	426	428	PF00400	0.583
TRG_ER_diArg_1	674	676	PF00400	0.501
TRG_ER_diArg_1	737	739	PF00400	0.596
TRG_ER_diArg_1	828	830	PF00400	0.539
TRG_ER_diArg_1	871	873	PF00400	0.816
TRG_Pf-PMV_PEXEL_1	556	561	PF00026	0.481
TRG_Pf-PMV_PEXEL_1	737	742	PF00026	0.481
TRG_Pf-PMV_PEXEL_1	828	833	PF00026	0.642

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A3Q8IF94	Leishmania donovani	51%	100%
A0A3Q8IJ79	Leishmania donovani	94%	100%
A4HMX7	Leishmania braziliensis	91%	100%
A4I7K4	Leishmania infantum	51%	100%
A4IBK1	Leishmania infantum	94%	100%
E9B2G1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	51%	100%
E9B6I9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	94%	100%
Q4Q5P5	Leishmania major	49%	100%