J domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DnaJ domain containing protein, putative

Species:

Leishmania major

UniProt:

E9AFD3_LEIMA

TriTrypDb:

LmjF.35.3090 , LMJLV39_350038200 , LMJSD75_350037600

Length:

427

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	9
GO:0031207	Sec62/Sec63 complex	3	2
GO:0032991	protein-containing complex	1	2
GO:0098796	membrane protein complex	2	2
GO:0110165	cellular anatomical entity	1	9
GO:0140534	endoplasmic reticulum protein-containing complex	2	2

Expansion

Sequence features

E9AFD3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFD3

Function

Biological processes
Term	Name	Level	Count
GO:0006605	protein targeting	5	2
GO:0006612	protein targeting to membrane	5	2
GO:0006613	cotranslational protein targeting to membrane	6	2
GO:0006614	SRP-dependent cotranslational protein targeting to membrane	7	2
GO:0006620	post-translational protein targeting to endoplasmic reticulum membrane	6	2
GO:0006810	transport	3	2
GO:0006886	intracellular protein transport	4	2
GO:0008104	protein localization	4	2
GO:0009987	cellular process	1	2
GO:0015031	protein transport	4	2
GO:0033036	macromolecule localization	2	2
GO:0033365	protein localization to organelle	5	2
GO:0045047	protein targeting to ER	6	2
GO:0045184	establishment of protein localization	3	2
GO:0046907	intracellular transport	3	2
GO:0051179	localization	1	2
GO:0051234	establishment of localization	2	2
GO:0051641	cellular localization	2	2
GO:0051649	establishment of localization in cell	3	2
GO:0051668	localization within membrane	3	2
GO:0070727	cellular macromolecule localization	3	2
GO:0070972	protein localization to endoplasmic reticulum	6	2
GO:0071702	organic substance transport	4	2
GO:0071705	nitrogen compound transport	4	2
GO:0072594	establishment of protein localization to organelle	4	2
GO:0072599	establishment of protein localization to endoplasmic reticulum	5	2
GO:0072657	protein localization to membrane	4	2
GO:0090150	establishment of protein localization to membrane	4	2

Molecular functions
Term	Name	Level	Count
GO:0005215	transporter activity	1	2
GO:0008320	protein transmembrane transporter activity	3	2
GO:0022857	transmembrane transporter activity	2	2
GO:0022884	macromolecule transmembrane transporter activity	3	2
GO:0140318	protein transporter activity	2	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	312	316	PF00656	0.438
CLV_NRD_NRD_1	116	118	PF00675	0.462
CLV_NRD_NRD_1	135	137	PF00675	0.320
CLV_NRD_NRD_1	186	188	PF00675	0.691
CLV_NRD_NRD_1	234	236	PF00675	0.636
CLV_NRD_NRD_1	238	240	PF00675	0.633
CLV_NRD_NRD_1	258	260	PF00675	0.416
CLV_NRD_NRD_1	3	5	PF00675	0.562
CLV_NRD_NRD_1	7	9	PF00675	0.568
CLV_NRD_NRD_1	87	89	PF00675	0.601
CLV_PCSK_FUR_1	133	137	PF00082	0.488
CLV_PCSK_FUR_1	97	101	PF00082	0.611
CLV_PCSK_KEX2_1	135	137	PF00082	0.454
CLV_PCSK_KEX2_1	184	186	PF00082	0.622
CLV_PCSK_KEX2_1	234	236	PF00082	0.637
CLV_PCSK_KEX2_1	278	280	PF00082	0.627
CLV_PCSK_KEX2_1	3	5	PF00082	0.562
CLV_PCSK_KEX2_1	404	406	PF00082	0.642
CLV_PCSK_KEX2_1	45	47	PF00082	0.325
CLV_PCSK_KEX2_1	7	9	PF00082	0.568
CLV_PCSK_KEX2_1	87	89	PF00082	0.598
CLV_PCSK_KEX2_1	99	101	PF00082	0.581
CLV_PCSK_PC1ET2_1	184	186	PF00082	0.614
CLV_PCSK_PC1ET2_1	278	280	PF00082	0.602
CLV_PCSK_PC1ET2_1	404	406	PF00082	0.627
CLV_PCSK_PC1ET2_1	45	47	PF00082	0.325
CLV_PCSK_PC1ET2_1	99	101	PF00082	0.525
CLV_PCSK_PC7_1	3	9	PF00082	0.428
CLV_PCSK_PC7_1	95	101	PF00082	0.530
CLV_PCSK_SKI1_1	141	145	PF00082	0.476
CLV_PCSK_SKI1_1	186	190	PF00082	0.691
CLV_PCSK_SKI1_1	234	238	PF00082	0.637
CLV_PCSK_SKI1_1	259	263	PF00082	0.528
CLV_PCSK_SKI1_1	353	357	PF00082	0.533
CLV_PCSK_SKI1_1	395	399	PF00082	0.639
CLV_PCSK_SKI1_1	7	11	PF00082	0.581
DEG_APCC_DBOX_1	140	148	PF00400	0.653
DEG_APCC_DBOX_1	184	192	PF00400	0.450
DEG_APCC_DBOX_1	2	10	PF00400	0.793
DEG_Nend_Nbox_1	1	3	PF02207	0.773
DEG_SCF_FBW7_1	418	424	PF00400	0.382
DEG_SCF_FBW7_2	29	35	PF00400	0.510
DOC_CKS1_1	29	34	PF01111	0.517
DOC_CKS1_1	418	423	PF01111	0.382
DOC_CYCLIN_RxL_1	361	371	PF00134	0.428
DOC_CYCLIN_yCln2_LP_2	195	201	PF00134	0.492
DOC_MAPK_gen_1	382	390	PF00069	0.383
DOC_PP1_RVXF_1	362	369	PF00149	0.421
DOC_PP2B_LxvP_1	195	198	PF13499	0.489
DOC_USP7_MATH_1	255	259	PF00917	0.411
DOC_USP7_MATH_1	421	425	PF00917	0.558
DOC_WW_Pin1_4	25	30	PF00397	0.523
DOC_WW_Pin1_4	306	311	PF00397	0.334
DOC_WW_Pin1_4	331	336	PF00397	0.390
DOC_WW_Pin1_4	417	422	PF00397	0.501
DOC_WW_Pin1_4	86	91	PF00397	0.819
DOC_WW_Pin1_4	99	104	PF00397	0.738
LIG_14-3-3_CanoR_1	135	144	PF00244	0.690
LIG_14-3-3_CanoR_1	173	178	PF00244	0.406
LIG_14-3-3_CanoR_1	234	241	PF00244	0.429
LIG_14-3-3_CanoR_1	259	266	PF00244	0.340
LIG_14-3-3_CanoR_1	3	7	PF00244	0.754
LIG_BRCT_BRCA1_1	262	266	PF00533	0.443
LIG_EH1_1	174	182	PF00400	0.448
LIG_FHA_1	160	166	PF00498	0.413
LIG_FHA_1	355	361	PF00498	0.393
LIG_FHA_2	204	210	PF00498	0.481
LIG_FHA_2	211	217	PF00498	0.470
LIG_FHA_2	29	35	PF00498	0.562
LIG_FHA_2	339	345	PF00498	0.313
LIG_FHA_2	407	413	PF00498	0.442
LIG_FHA_2	418	424	PF00498	0.500
LIG_LIR_Apic_2	308	314	PF02991	0.355
LIG_LIR_Gen_1	172	182	PF02991	0.381
LIG_LIR_Gen_1	209	217	PF02991	0.493
LIG_LIR_Gen_1	246	255	PF02991	0.350
LIG_LIR_Gen_1	31	41	PF02991	0.498
LIG_LIR_Gen_1	337	348	PF02991	0.340
LIG_LIR_Gen_1	63	73	PF02991	0.510
LIG_LIR_Nem_3	172	177	PF02991	0.359
LIG_LIR_Nem_3	209	214	PF02991	0.506
LIG_LIR_Nem_3	242	247	PF02991	0.363
LIG_LIR_Nem_3	292	298	PF02991	0.414
LIG_LIR_Nem_3	31	36	PF02991	0.510
LIG_LIR_Nem_3	337	343	PF02991	0.339
LIG_LIR_Nem_3	63	69	PF02991	0.445
LIG_NRBOX	143	149	PF00104	0.525
LIG_PTB_Apo_2	362	369	PF02174	0.335
LIG_SH2_CRK	174	178	PF00017	0.457
LIG_SH2_CRK	211	215	PF00017	0.482
LIG_SH2_CRK	295	299	PF00017	0.400
LIG_SH2_CRK	66	70	PF00017	0.476
LIG_SH2_STAT5	345	348	PF00017	0.417
LIG_SH3_3	194	200	PF00018	0.441
LIG_SH3_3	247	253	PF00018	0.373
LIG_SH3_3	26	32	PF00018	0.522
LIG_SUMO_SIM_anti_2	162	168	PF11976	0.381
LIG_SUMO_SIM_par_1	164	170	PF11976	0.368
LIG_SUMO_SIM_par_1	199	204	PF11976	0.477
LIG_TRAF2_1	129	132	PF00917	0.687
LIG_TRAF2_1	32	35	PF00917	0.558
MOD_CDK_SPxxK_3	86	93	PF00069	0.759
MOD_CK1_1	13	19	PF00069	0.738
MOD_CK1_1	159	165	PF00069	0.449
MOD_CK1_1	28	34	PF00069	0.476
MOD_CK1_1	334	340	PF00069	0.355
MOD_CK1_1	89	95	PF00069	0.760
MOD_CK2_1	210	216	PF00069	0.469
MOD_CK2_1	28	34	PF00069	0.577
MOD_CK2_1	338	344	PF00069	0.319
MOD_CK2_1	406	412	PF00069	0.447
MOD_CK2_1	417	423	PF00069	0.497
MOD_GlcNHglycan	14	18	PF01048	0.546
MOD_GlcNHglycan	311	314	PF01048	0.637
MOD_GlcNHglycan	61	65	PF01048	0.301
MOD_GlcNHglycan	80	83	PF01048	0.587
MOD_GSK3_1	135	142	PF00069	0.641
MOD_GSK3_1	255	262	PF00069	0.339
MOD_GSK3_1	285	292	PF00069	0.408
MOD_GSK3_1	331	338	PF00069	0.382
MOD_GSK3_1	417	424	PF00069	0.381
MOD_GSK3_1	74	81	PF00069	0.695
MOD_N-GLC_1	151	156	PF02516	0.452
MOD_N-GLC_1	335	340	PF02516	0.607
MOD_N-GLC_1	349	354	PF02516	0.544
MOD_NEK2_1	2	7	PF00069	0.778
MOD_NEK2_1	368	373	PF00069	0.451
MOD_NEK2_2	421	426	PF00069	0.562
MOD_PIKK_1	234	240	PF00454	0.436
MOD_PKA_1	135	141	PF00069	0.690
MOD_PKA_1	234	240	PF00069	0.436
MOD_PKA_1	259	265	PF00069	0.285
MOD_PKA_1	7	13	PF00069	0.632
MOD_PKA_2	135	141	PF00069	0.645
MOD_PKA_2	2	8	PF00069	0.753
MOD_PKA_2	234	240	PF00069	0.436
MOD_PKA_2	264	270	PF00069	0.380
MOD_PKA_2	73	79	PF00069	0.637
MOD_PKB_1	133	141	PF00069	0.667
MOD_PKB_1	226	234	PF00069	0.486
MOD_Plk_1	203	209	PF00069	0.453
MOD_Plk_1	228	234	PF00069	0.488
MOD_Plk_4	159	165	PF00069	0.459
MOD_Plk_4	318	324	PF00069	0.375
MOD_ProDKin_1	25	31	PF00069	0.523
MOD_ProDKin_1	306	312	PF00069	0.335
MOD_ProDKin_1	331	337	PF00069	0.390
MOD_ProDKin_1	417	423	PF00069	0.505
MOD_ProDKin_1	86	92	PF00069	0.818
MOD_ProDKin_1	99	105	PF00069	0.731
TRG_DiLeu_BaEn_2	138	144	PF01217	0.660
TRG_DiLeu_BaEn_2	145	151	PF01217	0.527
TRG_ENDOCYTIC_2	174	177	PF00928	0.362
TRG_ENDOCYTIC_2	211	214	PF00928	0.501
TRG_ENDOCYTIC_2	295	298	PF00928	0.366
TRG_ENDOCYTIC_2	345	348	PF00928	0.372
TRG_ENDOCYTIC_2	66	69	PF00928	0.476
TRG_ER_diArg_1	185	187	PF00400	0.429
TRG_ER_diArg_1	2	4	PF00400	0.792
TRG_ER_diArg_1	225	228	PF00400	0.445
TRG_ER_diArg_1	233	235	PF00400	0.418
TRG_ER_diArg_1	6	8	PF00400	0.794
TRG_Pf-PMV_PEXEL_1	179	183	PF00026	0.632
TRG_Pf-PMV_PEXEL_1	205	209	PF00026	0.677
TRG_Pf-PMV_PEXEL_1	364	369	PF00026	0.577
TRG_Pf-PMV_PEXEL_1	395	399	PF00026	0.657

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P559	Leptomonas seymouri	66%	99%
A0A0S4JM78	Bodo saltans	28%	100%
A0A1X0P5J7	Trypanosomatidae	48%	97%
A0A3Q8IJK4	Leishmania donovani	94%	100%
A0A422MXB6	Trypanosoma rangeli	45%	100%
A4HMX6	Leishmania braziliensis	80%	100%
A4IBK0	Leishmania infantum	94%	100%
C9ZZ44	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	100%
E9B6I8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	100%
V5DUR2	Trypanosoma cruzi	43%	100%