NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania major

UniProt:

E9AFC8_LEIMA

TriTrypDb:

LmjF.35.3040 , LMJLV39_350037700 , LMJSD75_350037100

Length:

271

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005747	mitochondrial respiratory chain complex I	4	2
GO:0030964	NADH dehydrogenase complex	3	2
GO:0032991	protein-containing complex	1	2
GO:0045271	respiratory chain complex I	4	2
GO:0098796	membrane protein complex	2	2
GO:0098798	mitochondrial protein-containing complex	2	2
GO:0098800	inner mitochondrial membrane protein complex	3	2
GO:0098803	respiratory chain complex	3	2
GO:1902494	catalytic complex	2	2
GO:1902495	transmembrane transporter complex	3	2
GO:1990204	oxidoreductase complex	3	2
GO:1990351	transporter complex	2	2
GO:0005743	mitochondrial inner membrane	5	9
GO:0016020	membrane	2	9
GO:0019866	organelle inner membrane	4	9
GO:0031090	organelle membrane	3	9
GO:0031966	mitochondrial membrane	4	9
GO:0070469	respirasome	2	9
GO:0110165	cellular anatomical entity	1	9

Expansion

Sequence features

E9AFC8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFC8

Function

Biological processes
Term	Name	Level	Count
GO:0006950	response to stress	2	2
GO:0006979	response to oxidative stress	3	2
GO:0009987	cellular process	1	11
GO:0010257	NADH dehydrogenase complex assembly	6	11
GO:0016043	cellular component organization	3	11
GO:0022607	cellular component assembly	4	11
GO:0032981	mitochondrial respiratory chain complex I assembly	7	11
GO:0033108	mitochondrial respiratory chain complex assembly	6	11
GO:0043933	protein-containing complex organization	4	11
GO:0050896	response to stimulus	1	2
GO:0065003	protein-containing complex assembly	5	11
GO:0071840	cellular component organization or biogenesis	2	11

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	115	117	PF00675	0.338
CLV_NRD_NRD_1	213	215	PF00675	0.497
CLV_NRD_NRD_1	83	85	PF00675	0.396
CLV_NRD_NRD_1	94	96	PF00675	0.385
CLV_PCSK_KEX2_1	115	117	PF00082	0.338
CLV_PCSK_KEX2_1	213	215	PF00082	0.515
CLV_PCSK_KEX2_1	94	96	PF00082	0.440
CLV_PCSK_SKI1_1	213	217	PF00082	0.510
DOC_CYCLIN_yClb1_LxF_4	57	62	PF00134	0.617
DOC_CYCLIN_yCln2_LP_2	173	179	PF00134	0.555
DOC_MAPK_gen_1	17	24	PF00069	0.577
DOC_MAPK_gen_1	94	102	PF00069	0.418
DOC_PP1_RVXF_1	57	63	PF00149	0.606
DOC_PP2B_LxvP_1	173	176	PF13499	0.552
DOC_USP7_MATH_1	23	27	PF00917	0.521
DOC_USP7_UBL2_3	28	32	PF12436	0.537
DOC_WW_Pin1_4	135	140	PF00397	0.305
DOC_WW_Pin1_4	154	159	PF00397	0.172
DOC_WW_Pin1_4	184	189	PF00397	0.558
LIG_14-3-3_CanoR_1	59	65	PF00244	0.521
LIG_BIR_II_1	1	5	PF00653	0.564
LIG_BRCT_BRCA1_1	1	5	PF00533	0.564
LIG_BRCT_BRCA1_1	30	34	PF00533	0.556
LIG_FHA_1	118	124	PF00498	0.414
LIG_FHA_1	202	208	PF00498	0.746
LIG_LIR_Gen_1	120	129	PF02991	0.371
LIG_LIR_Nem_3	120	125	PF02991	0.327
LIG_LIR_Nem_3	185	189	PF02991	0.526
LIG_LIR_Nem_3	73	77	PF02991	0.436
LIG_SH2_STAP1	242	246	PF00017	0.583
LIG_SH2_STAT3	251	254	PF00017	0.528
LIG_SH2_STAT5	121	124	PF00017	0.305
LIG_SH2_STAT5	195	198	PF00017	0.570
LIG_SH2_STAT5	36	39	PF00017	0.525
LIG_SH2_STAT5	88	91	PF00017	0.414
LIG_SH3_3	125	131	PF00018	0.289
LIG_SH3_3	229	235	PF00018	0.584
LIG_TRAF2_1	215	218	PF00917	0.515
LIG_UBA3_1	79	85	PF00899	0.379
MOD_CK1_1	3	9	PF00069	0.549
MOD_CMANNOS	146	149	PF00535	0.289
MOD_CMANNOS	64	67	PF00535	0.491
MOD_GSK3_1	24	31	PF00069	0.499
MOD_NEK2_1	34	39	PF00069	0.547
MOD_NEK2_1	60	65	PF00069	0.594
MOD_PKA_2	58	64	PF00069	0.513
MOD_Plk_4	117	123	PF00069	0.470
MOD_ProDKin_1	135	141	PF00069	0.305
MOD_ProDKin_1	154	160	PF00069	0.172
MOD_ProDKin_1	184	190	PF00069	0.557
MOD_SUMO_rev_2	91	101	PF00179	0.420
TRG_ENDOCYTIC_2	19	22	PF00928	0.487
TRG_ENDOCYTIC_2	233	236	PF00928	0.507
TRG_ER_diArg_1	93	95	PF00400	0.412
TRG_Pf-PMV_PEXEL_1	214	218	PF00026	0.499

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P4M1	Leptomonas seymouri	88%	100%
A0A0S4JK83	Bodo saltans	61%	100%
A0A1X0P5Q4	Trypanosomatidae	76%	100%
A0A3Q8II53	Leishmania donovani	97%	100%
A4HMX1	Leishmania braziliensis	95%	100%
A4IBJ2	Leishmania infantum	97%	100%
C9ZZ49	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	75%	100%
E9B6I3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	97%	100%
V5BY53	Trypanosoma cruzi	77%	100%