Protein-S-isoprenylcysteine O-methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

prenyl protein specific carboxyl methyltransferase, putative

Species:

Leishmania major

UniProt:

E9AFC7_LEIMA

TriTrypDb:

LmjF.35.3030 , LMJLV39_350037600 , LMJSD75_350037000

Length:

266

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005783	endoplasmic reticulum	5	2
GO:0005789	endoplasmic reticulum membrane	4	12
GO:0016020	membrane	2	12
GO:0031090	organelle membrane	3	12
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	12

Expansion

Sequence features

E9AFC7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFC7

Function

Biological processes
Term	Name	Level	Count
GO:0006479	protein methylation	4	2
GO:0006481	C-terminal protein methylation	5	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0008213	protein alkylation	5	2
GO:0009987	cellular process	1	2
GO:0018410	C-terminal protein amino acid modification	6	2
GO:0019538	protein metabolic process	3	2
GO:0032259	methylation	2	2
GO:0036211	protein modification process	4	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043412	macromolecule modification	4	2
GO:0043414	macromolecule methylation	3	2
GO:0043687	post-translational protein modification	5	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0071704	organic substance metabolic process	2	2
GO:1901564	organonitrogen compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0003880	protein C-terminal carboxyl O-methyltransferase activity	5	12
GO:0004671	protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity	6	12
GO:0008168	methyltransferase activity	4	12
GO:0008171	O-methyltransferase activity	5	12
GO:0008276	protein methyltransferase activity	3	12
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	12
GO:0010340	carboxyl-O-methyltransferase activity	6	12
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12
GO:0051998	protein carboxyl O-methyltransferase activity	4	12
GO:0140096	catalytic activity, acting on a protein	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	56	60	PF00656	0.286
CLV_C14_Caspase3-7	91	95	PF00656	0.517
CLV_NRD_NRD_1	18	20	PF00675	0.387
CLV_PCSK_KEX2_1	18	20	PF00082	0.366
CLV_PCSK_SKI1_1	132	136	PF00082	0.228
DOC_CDC14_PxL_1	99	107	PF14671	0.468
DOC_CYCLIN_RxL_1	22	32	PF00134	0.571
DOC_CYCLIN_yCln2_LP_2	195	201	PF00134	0.217
DOC_CYCLIN_yCln2_LP_2	214	220	PF00134	0.158
DOC_MAPK_gen_1	253	263	PF00069	0.443
DOC_MAPK_MEF2A_6	256	265	PF00069	0.456
DOC_PP4_FxxP_1	85	88	PF00568	0.523
LIG_14-3-3_CanoR_1	141	150	PF00244	0.242
LIG_14-3-3_CanoR_1	161	166	PF00244	0.166
LIG_BIR_II_1	1	5	PF00653	0.698
LIG_BRCT_BRCA1_1	145	149	PF00533	0.336
LIG_EH1_1	254	262	PF00400	0.448
LIG_FHA_1	108	114	PF00498	0.525
LIG_FHA_1	143	149	PF00498	0.313
LIG_FHA_1	186	192	PF00498	0.250
LIG_FHA_1	30	36	PF00498	0.316
LIG_FHA_2	54	60	PF00498	0.309
LIG_FHA_2	89	95	PF00498	0.490
LIG_LIR_Apic_2	118	123	PF02991	0.501
LIG_LIR_Gen_1	170	176	PF02991	0.262
LIG_LIR_Gen_1	94	104	PF02991	0.512
LIG_LIR_Nem_3	146	152	PF02991	0.253
LIG_LIR_Nem_3	227	231	PF02991	0.450
LIG_LIR_Nem_3	250	255	PF02991	0.449
LIG_LIR_Nem_3	94	99	PF02991	0.488
LIG_Pex14_2	201	205	PF04695	0.207
LIG_REV1ctd_RIR_1	136	145	PF16727	0.509
LIG_REV1ctd_RIR_1	228	238	PF16727	0.407
LIG_SH2_CRK	192	196	PF00017	0.235
LIG_SH2_NCK_1	120	124	PF00017	0.594
LIG_SH2_STAP1	144	148	PF00017	0.272
LIG_SH2_STAP1	237	241	PF00017	0.386
LIG_SH2_STAT5	104	107	PF00017	0.484
LIG_SH2_STAT5	144	147	PF00017	0.299
LIG_SH2_STAT5	194	197	PF00017	0.239
LIG_SH2_STAT5	200	203	PF00017	0.206
LIG_SH2_STAT5	229	232	PF00017	0.510
LIG_SH2_STAT5	247	250	PF00017	0.394
LIG_SH2_STAT5	67	70	PF00017	0.288
LIG_SH3_3	258	264	PF00018	0.462
LIG_SUMO_SIM_anti_2	151	157	PF11976	0.329
LIG_TRAF2_1	236	239	PF00917	0.411
MOD_CK1_1	170	176	PF00069	0.258
MOD_CK1_1	2	8	PF00069	0.628
MOD_CK2_1	2	8	PF00069	0.562
MOD_GlcNHglycan	107	110	PF01048	0.281
MOD_GlcNHglycan	145	148	PF01048	0.407
MOD_GlcNHglycan	181	184	PF01048	0.528
MOD_GlcNHglycan	218	221	PF01048	0.320
MOD_GlcNHglycan	4	7	PF01048	0.390
MOD_GSK3_1	103	110	PF00069	0.514
MOD_N-GLC_1	142	147	PF02516	0.233
MOD_N-GLC_1	170	175	PF02516	0.407
MOD_NEK2_1	1	6	PF00069	0.631
MOD_NEK2_1	105	110	PF00069	0.474
MOD_NEK2_1	148	153	PF00069	0.313
MOD_NEK2_1	204	209	PF00069	0.230
MOD_NEK2_1	230	235	PF00069	0.499
MOD_PKA_2	179	185	PF00069	0.219
MOD_PKA_2	88	94	PF00069	0.433
MOD_Plk_1	170	176	PF00069	0.207
MOD_Plk_1	237	243	PF00069	0.447
MOD_Plk_4	161	167	PF00069	0.324
MOD_Plk_4	221	227	PF00069	0.326
TRG_ENDOCYTIC_2	192	195	PF00928	0.239
TRG_ER_diArg_1	17	19	PF00400	0.630
TRG_ER_diArg_1	231	234	PF00400	0.512
TRG_Pf-PMV_PEXEL_1	26	31	PF00026	0.274

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P511	Leptomonas seymouri	65%	100%
A0A0S4JHW1	Bodo saltans	45%	100%
A0A1X0P5F1	Trypanosomatidae	51%	100%
A0A3S7X9G8	Leishmania donovani	93%	100%
A0A422P2Y4	Trypanosoma rangeli	50%	100%
A2XX73	Oryza sativa subsp. indica	34%	100%
A4HMX0	Leishmania braziliensis	83%	100%
A4IBJ1	Leishmania infantum	93%	100%
C9ZZ50	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	46%	100%
D6WJ77	Tribolium castaneum	34%	95%
E9B6I2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	100%
O12947	Xenopus laevis	31%	92%
O60725	Homo sapiens	30%	94%
P32584	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	31%	100%
P87014	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	35%	100%
Q558K8	Dictyostelium discoideum	33%	100%
Q7XSR9	Oryza sativa subsp. japonica	34%	100%
Q9EQK7	Mus musculus	32%	94%
Q9WVM4	Rattus norvegicus	32%	94%
V5C2X9	Trypanosoma cruzi	48%	100%