3'-5' exonuclease domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

3'-5' exonuclease, putative

Species:

Leishmania major

UniProt:

E9AFC3_LEIMA

TriTrypDb:

LmjF.35.2990 , LMJLV39_350037200 , LMJSD75_350036500

Length:

412

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

E9AFC3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFC3

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003824	catalytic activity	1	12
GO:0004518	nuclease activity	4	12
GO:0004527	exonuclease activity	5	12
GO:0005488	binding	1	12
GO:0008270	zinc ion binding	6	12
GO:0008408	3'-5' exonuclease activity	6	12
GO:0016787	hydrolase activity	2	12
GO:0016788	hydrolase activity, acting on ester bonds	3	12
GO:0043167	ion binding	2	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0046914	transition metal ion binding	5	12
GO:0097159	organic cyclic compound binding	2	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	302	306	PF00656	0.454
CLV_MEL_PAP_1	62	68	PF00089	0.626
CLV_NRD_NRD_1	165	167	PF00675	0.275
CLV_NRD_NRD_1	173	175	PF00675	0.268
CLV_NRD_NRD_1	188	190	PF00675	0.276
CLV_NRD_NRD_1	209	211	PF00675	0.276
CLV_NRD_NRD_1	340	342	PF00675	0.524
CLV_PCSK_KEX2_1	173	175	PF00082	0.279
CLV_PCSK_KEX2_1	188	190	PF00082	0.276
CLV_PCSK_KEX2_1	209	211	PF00082	0.276
CLV_PCSK_PC7_1	205	211	PF00082	0.287
CLV_PCSK_SKI1_1	157	161	PF00082	0.287
CLV_PCSK_SKI1_1	166	170	PF00082	0.245
CLV_PCSK_SKI1_1	188	192	PF00082	0.381
DEG_Nend_UBRbox_1	1	4	PF02207	0.715
DEG_SPOP_SBC_1	32	36	PF00917	0.768
DOC_ANK_TNKS_1	317	324	PF00023	0.404
DOC_CKS1_1	112	117	PF01111	0.553
DOC_CKS1_1	118	123	PF01111	0.553
DOC_CKS1_1	29	34	PF01111	0.697
DOC_CYCLIN_RxL_1	154	164	PF00134	0.514
DOC_CYCLIN_yCln2_LP_2	147	153	PF00134	0.524
DOC_CYCLIN_yCln2_LP_2	79	85	PF00134	0.404
DOC_MAPK_gen_1	101	109	PF00069	0.495
DOC_MAPK_gen_1	188	195	PF00069	0.581
DOC_MAPK_MEF2A_6	101	109	PF00069	0.501
DOC_MAPK_MEF2A_6	188	197	PF00069	0.492
DOC_MAPK_NFAT4_5	188	196	PF00069	0.581
DOC_PP1_RVXF_1	164	171	PF00149	0.487
DOC_PP2B_LxvP_1	147	150	PF13499	0.484
DOC_PP4_FxxP_1	112	115	PF00568	0.553
DOC_USP7_MATH_1	32	36	PF00917	0.695
DOC_USP7_UBL2_3	80	84	PF12436	0.473
DOC_WW_Pin1_4	111	116	PF00397	0.560
DOC_WW_Pin1_4	117	122	PF00397	0.565
DOC_WW_Pin1_4	183	188	PF00397	0.525
DOC_WW_Pin1_4	19	24	PF00397	0.725
DOC_WW_Pin1_4	28	33	PF00397	0.662
DOC_WW_Pin1_4	60	65	PF00397	0.605
LIG_14-3-3_CanoR_1	125	129	PF00244	0.501
LIG_14-3-3_CanoR_1	210	216	PF00244	0.501
LIG_14-3-3_CanoR_1	25	33	PF00244	0.710
LIG_14-3-3_CanoR_1	285	291	PF00244	0.391
LIG_14-3-3_CanoR_1	348	352	PF00244	0.449
LIG_14-3-3_CanoR_1	56	66	PF00244	0.655
LIG_BIR_III_2	305	309	PF00653	0.491
LIG_BRCT_BRCA1_1	267	271	PF00533	0.508
LIG_BRCT_BRCA1_1	62	66	PF00533	0.633
LIG_FHA_1	1	7	PF00498	0.662
LIG_FHA_1	118	124	PF00498	0.538
LIG_FHA_1	212	218	PF00498	0.517
LIG_FHA_1	244	250	PF00498	0.516
LIG_FHA_1	273	279	PF00498	0.544
LIG_FHA_1	311	317	PF00498	0.529
LIG_FHA_2	129	135	PF00498	0.558
LIG_FHA_2	179	185	PF00498	0.525
LIG_FHA_2	333	339	PF00498	0.440
LIG_FHA_2	50	56	PF00498	0.747
LIG_FHA_2	86	92	PF00498	0.581
LIG_LIR_Apic_2	110	115	PF02991	0.553
LIG_LIR_Apic_2	317	322	PF02991	0.395
LIG_LIR_Gen_1	57	66	PF02991	0.686
LIG_LIR_Nem_3	251	256	PF02991	0.476
LIG_LIR_Nem_3	268	274	PF02991	0.420
LIG_LIR_Nem_3	337	343	PF02991	0.547
LIG_LIR_Nem_3	350	354	PF02991	0.378
LIG_LIR_Nem_3	57	62	PF02991	0.745
LIG_LIR_Nem_3	63	69	PF02991	0.556
LIG_MLH1_MIPbox_1	267	271	PF16413	0.508
LIG_Pex14_1	315	319	PF04695	0.396
LIG_Rb_pABgroove_1	136	144	PF01858	0.487
LIG_REV1ctd_RIR_1	167	178	PF16727	0.537
LIG_SH2_CRK	83	87	PF00017	0.537
LIG_SH2_NCK_1	7	11	PF00017	0.644
LIG_SH2_PTP2	319	322	PF00017	0.413
LIG_SH2_STAP1	7	11	PF00017	0.605
LIG_SH2_STAT5	215	218	PF00017	0.486
LIG_SH2_STAT5	270	273	PF00017	0.463
LIG_SH2_STAT5	290	293	PF00017	0.394
LIG_SH2_STAT5	319	322	PF00017	0.392
LIG_SUMO_SIM_par_1	143	148	PF11976	0.477
LIG_TRAF2_1	88	91	PF00917	0.525
LIG_WRC_WIRS_1	325	330	PF05994	0.558
MOD_CDK_SPK_2	183	188	PF00069	0.525
MOD_CDK_SPK_2	60	65	PF00069	0.495
MOD_CDK_SPxK_1	183	189	PF00069	0.525
MOD_CDK_SPxK_1	19	25	PF00069	0.755
MOD_CK1_1	211	217	PF00069	0.501
MOD_CK1_1	28	34	PF00069	0.726
MOD_CK1_1	43	49	PF00069	0.554
MOD_CK1_1	60	66	PF00069	0.445
MOD_CK2_1	332	338	PF00069	0.442
MOD_CK2_1	49	55	PF00069	0.665
MOD_CK2_1	85	91	PF00069	0.538
MOD_GlcNHglycan	17	20	PF01048	0.715
MOD_GlcNHglycan	210	213	PF01048	0.287
MOD_GlcNHglycan	229	232	PF01048	0.287
MOD_GlcNHglycan	35	38	PF01048	0.602
MOD_GlcNHglycan	45	48	PF01048	0.720
MOD_GlcNHglycan	71	74	PF01048	0.463
MOD_GSK3_1	124	131	PF00069	0.557
MOD_GSK3_1	15	22	PF00069	0.676
MOD_GSK3_1	227	234	PF00069	0.501
MOD_GSK3_1	23	30	PF00069	0.644
MOD_GSK3_1	266	273	PF00069	0.615
MOD_GSK3_1	324	331	PF00069	0.535
MOD_GSK3_1	60	67	PF00069	0.608
MOD_NEK2_1	128	133	PF00069	0.550
MOD_NEK2_1	145	150	PF00069	0.405
MOD_NEK2_1	15	20	PF00069	0.669
MOD_NEK2_1	195	200	PF00069	0.517
MOD_NEK2_1	266	271	PF00069	0.530
MOD_NEK2_1	328	333	PF00069	0.504
MOD_NEK2_1	33	38	PF00069	0.576
MOD_NEK2_1	347	352	PF00069	0.419
MOD_NEK2_1	378	383	PF00069	0.456
MOD_NEK2_1	99	104	PF00069	0.530
MOD_PIKK_1	178	184	PF00454	0.516
MOD_PIKK_1	25	31	PF00454	0.772
MOD_PIKK_1	272	278	PF00454	0.546
MOD_PK_1	203	209	PF00069	0.487
MOD_PKA_2	124	130	PF00069	0.501
MOD_PKA_2	208	214	PF00069	0.476
MOD_PKA_2	284	290	PF00069	0.390
MOD_PKA_2	347	353	PF00069	0.449
MOD_PKA_2	55	61	PF00069	0.677
MOD_PKA_2	64	70	PF00069	0.470
MOD_Plk_1	161	167	PF00069	0.581
MOD_Plk_1	54	60	PF00069	0.565
MOD_Plk_4	124	130	PF00069	0.537
MOD_Plk_4	211	217	PF00069	0.501
MOD_Plk_4	266	272	PF00069	0.590
MOD_ProDKin_1	111	117	PF00069	0.560
MOD_ProDKin_1	183	189	PF00069	0.525
MOD_ProDKin_1	19	25	PF00069	0.728
MOD_ProDKin_1	28	34	PF00069	0.660
MOD_ProDKin_1	60	66	PF00069	0.593
MOD_SUMO_rev_2	87	95	PF00179	0.581
TRG_ENDOCYTIC_2	83	86	PF00928	0.476
TRG_ER_diArg_1	187	189	PF00400	0.470
TRG_Pf-PMV_PEXEL_1	157	162	PF00026	0.282
TRG_Pf-PMV_PEXEL_1	166	171	PF00026	0.271
TRG_Pf-PMV_PEXEL_1	189	194	PF00026	0.362
TRG_Pf-PMV_PEXEL_1	247	251	PF00026	0.300
TRG_Pf-PMV_PEXEL_1	25	29	PF00026	0.771

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HWS9	Leptomonas seymouri	78%	97%
A0A0S4JGY9	Bodo saltans	50%	100%
A0A1X0P5F6	Trypanosomatidae	68%	100%
A0A3R7KR79	Trypanosoma rangeli	69%	100%
A0A3S7X9G9	Leishmania donovani	96%	100%
A4HMW6	Leishmania braziliensis	88%	100%
A4IBI7	Leishmania infantum	95%	100%
C9ZZ53	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	69%	100%
E9B6H8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%
V5BY58	Trypanosoma cruzi	68%	100%