SpoU_methylase domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

SpoU rRNA Methylase family, putative

Species:

Leishmania major

UniProt:

E9AFB3_LEIMA

TriTrypDb:

LmjF.35.2890 , LMJLV39_350036100 , LMJSD75_350035400

Length:

569

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

E9AFB3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFB3

PDB structure(s): 7am2_BX , 7am2_BY

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	9
GO:0006396	RNA processing	6	9
GO:0006725	cellular aromatic compound metabolic process	3	9
GO:0006807	nitrogen compound metabolic process	2	9
GO:0008152	metabolic process	1	10
GO:0009987	cellular process	1	9
GO:0016070	RNA metabolic process	5	9
GO:0034641	cellular nitrogen compound metabolic process	3	9
GO:0043170	macromolecule metabolic process	3	9
GO:0044237	cellular metabolic process	2	9
GO:0044238	primary metabolic process	2	9
GO:0046483	heterocycle metabolic process	3	9
GO:0071704	organic substance metabolic process	2	9
GO:0090304	nucleic acid metabolic process	4	9
GO:1901360	organic cyclic compound metabolic process	3	9
GO:0032259	methylation	2	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	9
GO:0003723	RNA binding	4	9
GO:0003824	catalytic activity	1	10
GO:0005488	binding	1	9
GO:0008168	methyltransferase activity	4	10
GO:0008173	RNA methyltransferase activity	4	9
GO:0016740	transferase activity	2	10
GO:0016741	transferase activity, transferring one-carbon groups	3	10
GO:0097159	organic cyclic compound binding	2	9
GO:0140098	catalytic activity, acting on RNA	3	9
GO:0140640	catalytic activity, acting on a nucleic acid	2	9
GO:1901363	heterocyclic compound binding	2	9

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	142	144	PF00675	0.517
CLV_NRD_NRD_1	384	386	PF00675	0.430
CLV_NRD_NRD_1	392	394	PF00675	0.425
CLV_NRD_NRD_1	487	489	PF00675	0.466
CLV_NRD_NRD_1	492	494	PF00675	0.481
CLV_NRD_NRD_1	506	508	PF00675	0.419
CLV_NRD_NRD_1	513	515	PF00675	0.439
CLV_NRD_NRD_1	518	520	PF00675	0.430
CLV_PCSK_FUR_1	485	489	PF00082	0.419
CLV_PCSK_FUR_1	516	520	PF00082	0.589
CLV_PCSK_KEX2_1	142	144	PF00082	0.611
CLV_PCSK_KEX2_1	379	381	PF00082	0.420
CLV_PCSK_KEX2_1	384	386	PF00082	0.406
CLV_PCSK_KEX2_1	392	394	PF00082	0.400
CLV_PCSK_KEX2_1	487	489	PF00082	0.420
CLV_PCSK_KEX2_1	491	493	PF00082	0.443
CLV_PCSK_KEX2_1	506	508	PF00082	0.435
CLV_PCSK_KEX2_1	513	515	PF00082	0.437
CLV_PCSK_KEX2_1	518	520	PF00082	0.432
CLV_PCSK_PC1ET2_1	379	381	PF00082	0.420
CLV_PCSK_PC7_1	380	386	PF00082	0.424
CLV_PCSK_PC7_1	388	394	PF00082	0.425
CLV_PCSK_PC7_1	487	493	PF00082	0.530
CLV_PCSK_PC7_1	514	520	PF00082	0.513
CLV_PCSK_SKI1_1	103	107	PF00082	0.375
CLV_PCSK_SKI1_1	27	31	PF00082	0.524
CLV_PCSK_SKI1_1	380	384	PF00082	0.599
CLV_PCSK_SKI1_1	396	400	PF00082	0.382
CLV_PCSK_SKI1_1	527	531	PF00082	0.719
CLV_PCSK_SKI1_1	551	555	PF00082	0.477
CLV_PCSK_SKI1_1	62	66	PF00082	0.400
DEG_APCC_DBOX_1	266	274	PF00400	0.359
DEG_APCC_DBOX_1	379	387	PF00400	0.600
DEG_APCC_DBOX_1	395	403	PF00400	0.407
DEG_ODPH_VHL_1	19	31	PF01847	0.475
DEG_SPOP_SBC_1	42	46	PF00917	0.636
DOC_CKS1_1	171	176	PF01111	0.508
DOC_CYCLIN_RxL_1	184	197	PF00134	0.398
DOC_MAPK_gen_1	142	150	PF00069	0.401
DOC_MAPK_gen_1	392	399	PF00069	0.467
DOC_MAPK_gen_1	516	526	PF00069	0.479
DOC_MAPK_gen_1	57	65	PF00069	0.385
DOC_MAPK_gen_1	98	107	PF00069	0.358
DOC_MAPK_JIP1_4	123	129	PF00069	0.483
DOC_MAPK_MEF2A_6	448	456	PF00069	0.532
DOC_PP1_RVXF_1	69	75	PF00149	0.341
DOC_PP2B_LxvP_1	319	322	PF13499	0.547
DOC_PP4_FxxP_1	169	172	PF00568	0.367
DOC_PP4_FxxP_1	373	376	PF00568	0.478
DOC_USP7_MATH_1	322	326	PF00917	0.606
DOC_USP7_MATH_1	338	342	PF00917	0.412
DOC_USP7_MATH_1	38	42	PF00917	0.562
DOC_USP7_MATH_1	403	407	PF00917	0.553
DOC_USP7_MATH_1	8	12	PF00917	0.546
DOC_USP7_MATH_2	405	411	PF00917	0.545
DOC_USP7_UBL2_3	58	62	PF12436	0.374
DOC_WW_Pin1_4	156	161	PF00397	0.448
DOC_WW_Pin1_4	170	175	PF00397	0.420
DOC_WW_Pin1_4	295	300	PF00397	0.712
DOC_WW_Pin1_4	401	406	PF00397	0.548
LIG_14-3-3_CanoR_1	142	150	PF00244	0.468
LIG_14-3-3_CanoR_1	152	157	PF00244	0.384
LIG_14-3-3_CanoR_1	308	312	PF00244	0.567
LIG_14-3-3_CanoR_1	323	329	PF00244	0.413
LIG_14-3-3_CanoR_1	392	399	PF00244	0.414
LIG_14-3-3_CanoR_1	458	464	PF00244	0.600
LIG_14-3-3_CanoR_1	546	550	PF00244	0.616
LIG_14-3-3_CanoR_1	9	19	PF00244	0.650
LIG_Actin_WH2_2	79	94	PF00022	0.299
LIG_BIR_III_4	280	284	PF00653	0.402
LIG_CSL_BTD_1	522	525	PF09270	0.691
LIG_FHA_1	143	149	PF00498	0.427
LIG_FHA_1	28	34	PF00498	0.553
LIG_FHA_1	291	297	PF00498	0.656
LIG_FHA_1	393	399	PF00498	0.392
LIG_FHA_1	440	446	PF00498	0.522
LIG_FHA_2	246	252	PF00498	0.532
LIG_FHA_2	434	440	PF00498	0.617
LIG_FHA_2	75	81	PF00498	0.397
LIG_LIR_Apic_2	13	18	PF02991	0.635
LIG_LIR_Apic_2	168	172	PF02991	0.363
LIG_LIR_Apic_2	561	567	PF02991	0.564
LIG_LIR_Gen_1	368	376	PF02991	0.483
LIG_LIR_Nem_3	255	261	PF02991	0.364
LIG_LIR_Nem_3	368	373	PF02991	0.481
LIG_LIR_Nem_3	430	434	PF02991	0.483
LIG_NRBOX	201	207	PF00104	0.446
LIG_NRP_CendR_1	566	569	PF00754	0.581
LIG_PCNA_yPIPBox_3	505	519	PF02747	0.557
LIG_RPA_C_Fungi	502	514	PF08784	0.453
LIG_SH2_CRK	214	218	PF00017	0.282
LIG_SH2_CRK	258	262	PF00017	0.358
LIG_SH2_CRK	564	568	PF00017	0.681
LIG_SH2_SRC	431	434	PF00017	0.563
LIG_SH2_STAP1	214	218	PF00017	0.282
LIG_SH2_STAP1	227	231	PF00017	0.282
LIG_SH2_STAP1	329	333	PF00017	0.435
LIG_SH2_STAP1	497	501	PF00017	0.427
LIG_SH2_STAT3	275	278	PF00017	0.438
LIG_SH2_STAT5	165	168	PF00017	0.404
LIG_SH2_STAT5	247	250	PF00017	0.367
LIG_SH2_STAT5	275	278	PF00017	0.459
LIG_SH2_STAT5	370	373	PF00017	0.466
LIG_SH2_STAT5	421	424	PF00017	0.397
LIG_SH2_STAT5	431	434	PF00017	0.408
LIG_SH3_1	15	21	PF00018	0.522
LIG_SH3_1	519	525	PF00018	0.498
LIG_SH3_2	172	177	PF14604	0.398
LIG_SH3_2	522	527	PF14604	0.525
LIG_SH3_3	15	21	PF00018	0.527
LIG_SH3_3	169	175	PF00018	0.468
LIG_SH3_3	26	32	PF00018	0.530
LIG_SH3_3	293	299	PF00018	0.691
LIG_SH3_3	314	320	PF00018	0.627
LIG_SH3_3	519	525	PF00018	0.619
LIG_SH3_CIN85_PxpxPR_1	318	323	PF14604	0.598
LIG_SUMO_SIM_anti_2	145	151	PF11976	0.467
LIG_SUMO_SIM_par_1	145	151	PF11976	0.387
LIG_SUMO_SIM_par_1	189	194	PF11976	0.320
LIG_TRAF2_1	343	346	PF00917	0.575
LIG_TRFH_1	421	425	PF08558	0.415
LIG_UBA3_1	64	71	PF00899	0.333
MOD_CDK_SPxxK_3	170	177	PF00069	0.453
MOD_CK1_1	325	331	PF00069	0.576
MOD_CK1_1	391	397	PF00069	0.419
MOD_CK1_1	401	407	PF00069	0.494
MOD_CK1_1	41	47	PF00069	0.616
MOD_CK2_1	294	300	PF00069	0.613
MOD_CK2_1	357	363	PF00069	0.552
MOD_CK2_1	401	407	PF00069	0.573
MOD_CK2_1	433	439	PF00069	0.618
MOD_GlcNHglycan	12	15	PF01048	0.606
MOD_GlcNHglycan	209	212	PF01048	0.342
MOD_GlcNHglycan	360	363	PF01048	0.692
MOD_GlcNHglycan	367	370	PF01048	0.487
MOD_GlcNHglycan	40	43	PF01048	0.649
MOD_GlcNHglycan	45	48	PF01048	0.574
MOD_GlcNHglycan	51	54	PF01048	0.567
MOD_GSK3_1	152	159	PF00069	0.525
MOD_GSK3_1	201	208	PF00069	0.282
MOD_GSK3_1	290	297	PF00069	0.692
MOD_GSK3_1	325	332	PF00069	0.585
MOD_GSK3_1	354	361	PF00069	0.636
MOD_GSK3_1	38	45	PF00069	0.607
MOD_GSK3_1	388	395	PF00069	0.515
MOD_GSK3_1	403	410	PF00069	0.532
MOD_LATS_1	140	146	PF00433	0.403
MOD_N-GLC_1	27	32	PF02516	0.525
MOD_N-GLC_2	221	223	PF02516	0.282
MOD_NEK2_1	10	15	PF00069	0.562
MOD_NEK2_1	205	210	PF00069	0.315
MOD_NEK2_1	240	245	PF00069	0.316
MOD_NEK2_1	307	312	PF00069	0.625
MOD_NEK2_1	386	391	PF00069	0.542
MOD_NEK2_1	398	403	PF00069	0.555
MOD_NEK2_1	91	96	PF00069	0.518
MOD_NEK2_2	245	250	PF00069	0.391
MOD_PIKK_1	22	28	PF00454	0.595
MOD_PIKK_1	288	294	PF00454	0.533
MOD_PIKK_1	338	344	PF00454	0.654
MOD_PK_1	152	158	PF00069	0.468
MOD_PK_1	448	454	PF00069	0.475
MOD_PKA_1	142	148	PF00069	0.481
MOD_PKA_1	392	398	PF00069	0.466
MOD_PKA_1	492	498	PF00069	0.499
MOD_PKA_2	142	148	PF00069	0.594
MOD_PKA_2	307	313	PF00069	0.567
MOD_PKA_2	322	328	PF00069	0.416
MOD_PKA_2	33	39	PF00069	0.657
MOD_PKA_2	354	360	PF00069	0.493
MOD_PKA_2	391	397	PF00069	0.434
MOD_PKA_2	459	465	PF00069	0.552
MOD_PKA_2	492	498	PF00069	0.574
MOD_PKA_2	545	551	PF00069	0.620
MOD_PKA_2	8	14	PF00069	0.635
MOD_PKB_1	556	564	PF00069	0.398
MOD_Plk_1	150	156	PF00069	0.398
MOD_Plk_1	27	33	PF00069	0.526
MOD_Plk_1	325	331	PF00069	0.536
MOD_Plk_1	386	392	PF00069	0.335
MOD_Plk_1	438	444	PF00069	0.593
MOD_Plk_2-3	439	445	PF00069	0.550
MOD_Plk_4	201	207	PF00069	0.282
MOD_Plk_4	307	313	PF00069	0.600
MOD_Plk_4	414	420	PF00069	0.439
MOD_Plk_4	74	80	PF00069	0.379
MOD_ProDKin_1	156	162	PF00069	0.447
MOD_ProDKin_1	170	176	PF00069	0.422
MOD_ProDKin_1	295	301	PF00069	0.708
MOD_ProDKin_1	401	407	PF00069	0.548
MOD_SUMO_for_1	249	252	PF00179	0.412
TRG_DiLeu_BaEn_1	266	271	PF01217	0.459
TRG_DiLeu_BaEn_2	478	484	PF01217	0.384
TRG_DiLeu_BaLyEn_6	393	398	PF01217	0.501
TRG_ENDOCYTIC_2	214	217	PF00928	0.282
TRG_ENDOCYTIC_2	258	261	PF00928	0.359
TRG_ENDOCYTIC_2	370	373	PF00928	0.485
TRG_ENDOCYTIC_2	431	434	PF00928	0.481
TRG_ER_diArg_1	383	385	PF00400	0.433
TRG_ER_diArg_1	423	426	PF00400	0.468
TRG_ER_diArg_1	458	461	PF00400	0.499
TRG_ER_diArg_1	486	488	PF00400	0.450
TRG_ER_diArg_1	491	493	PF00400	0.467
TRG_ER_diArg_1	506	508	PF00400	0.308
TRG_ER_diArg_1	512	514	PF00400	0.439
TRG_ER_diArg_1	516	519	PF00400	0.434
TRG_Pf-PMV_PEXEL_1	125	130	PF00026	0.447
TRG_Pf-PMV_PEXEL_1	189	194	PF00026	0.282
TRG_Pf-PMV_PEXEL_1	396	400	PF00026	0.515
TRG_Pf-PMV_PEXEL_1	71	75	PF00026	0.415

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PEE8	Leptomonas seymouri	70%	93%
A0A1X0P5S3	Trypanosomatidae	50%	98%
A0A3S7X9F7	Leishmania donovani	94%	94%
A0A422P2S4	Trypanosoma rangeli	50%	100%
A4HMV6	Leishmania braziliensis	80%	100%
A4IBH7	Leishmania infantum	94%	94%
C9ZZ65	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	50%	97%
E9B6G8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	94%