Protein kinase domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

protein kinase, putative

Species:

Leishmania major

UniProt:

E9AFB1_LEIMA

TriTrypDb:

LmjF.35.2870 , LMJLV39_350035900 , LMJSD75_350035200

Length:

740

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 6
NetGPI	no	yes: 0, no: 6

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

E9AFB1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFB1

Function

Biological processes
Term	Name	Level	Count
GO:0006468	protein phosphorylation	5	7
GO:0006793	phosphorus metabolic process	3	7
GO:0006796	phosphate-containing compound metabolic process	4	7
GO:0006807	nitrogen compound metabolic process	2	7
GO:0008152	metabolic process	1	7
GO:0009987	cellular process	1	7
GO:0016310	phosphorylation	5	7
GO:0019538	protein metabolic process	3	7
GO:0036211	protein modification process	4	7
GO:0043170	macromolecule metabolic process	3	7
GO:0043412	macromolecule modification	4	7
GO:0044237	cellular metabolic process	2	7
GO:0044238	primary metabolic process	2	7
GO:0071704	organic substance metabolic process	2	7
GO:1901564	organonitrogen compound metabolic process	3	7

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	7
GO:0003824	catalytic activity	1	7
GO:0004672	protein kinase activity	3	7
GO:0004674	protein serine/threonine kinase activity	4	2
GO:0005488	binding	1	7
GO:0005524	ATP binding	5	7
GO:0016301	kinase activity	4	7
GO:0016740	transferase activity	2	7
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	7
GO:0016773	phosphotransferase activity, alcohol group as acceptor	4	7
GO:0017076	purine nucleotide binding	4	7
GO:0030554	adenyl nucleotide binding	5	7
GO:0032553	ribonucleotide binding	3	7
GO:0032555	purine ribonucleotide binding	4	7
GO:0032559	adenyl ribonucleotide binding	5	7
GO:0035639	purine ribonucleoside triphosphate binding	4	7
GO:0036094	small molecule binding	2	7
GO:0043167	ion binding	2	7
GO:0043168	anion binding	3	7
GO:0097159	organic cyclic compound binding	2	7
GO:0097367	carbohydrate derivative binding	2	7
GO:0140096	catalytic activity, acting on a protein	2	7
GO:1901265	nucleoside phosphate binding	3	7
GO:1901363	heterocyclic compound binding	2	7

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	112	116	PF00656	0.556
CLV_C14_Caspase3-7	162	166	PF00656	0.572
CLV_C14_Caspase3-7	203	207	PF00656	0.434
CLV_NRD_NRD_1	102	104	PF00675	0.592
CLV_NRD_NRD_1	444	446	PF00675	0.507
CLV_NRD_NRD_1	671	673	PF00675	0.595
CLV_NRD_NRD_1	727	729	PF00675	0.567
CLV_NRD_NRD_1	730	732	PF00675	0.548
CLV_PCSK_FUR_1	100	104	PF00082	0.564
CLV_PCSK_KEX2_1	102	104	PF00082	0.563
CLV_PCSK_KEX2_1	405	407	PF00082	0.387
CLV_PCSK_KEX2_1	444	446	PF00082	0.507
CLV_PCSK_PC1ET2_1	405	407	PF00082	0.366
CLV_PCSK_SKI1_1	422	426	PF00082	0.525
CLV_PCSK_SKI1_1	496	500	PF00082	0.547
CLV_PCSK_SKI1_1	522	526	PF00082	0.545
CLV_PCSK_SKI1_1	596	600	PF00082	0.613
CLV_PCSK_SKI1_1	733	737	PF00082	0.607
DEG_APCC_DBOX_1	405	413	PF00400	0.430
DEG_SPOP_SBC_1	126	130	PF00917	0.623
DEG_SPOP_SBC_1	293	297	PF00917	0.589
DEG_SPOP_SBC_1	716	720	PF00917	0.546
DEG_SPOP_SBC_1	75	79	PF00917	0.622
DOC_CDC14_PxL_1	312	320	PF14671	0.371
DOC_CDC14_PxL_1	46	54	PF14671	0.523
DOC_CKS1_1	489	494	PF01111	0.589
DOC_CYCLIN_yCln2_LP_2	376	382	PF00134	0.443
DOC_MAPK_gen_1	405	413	PF00069	0.441
DOC_MAPK_MEF2A_6	260	269	PF00069	0.424
DOC_MAPK_MEF2A_6	496	504	PF00069	0.493
DOC_MAPK_RevD_3	89	103	PF00069	0.612
DOC_MIT_MIM_1	619	629	PF04212	0.569
DOC_PP2B_LxvP_1	149	152	PF13499	0.542
DOC_USP7_MATH_1	163	167	PF00917	0.701
DOC_USP7_MATH_1	457	461	PF00917	0.571
DOC_USP7_MATH_1	565	569	PF00917	0.516
DOC_USP7_MATH_1	572	576	PF00917	0.546
DOC_USP7_MATH_1	663	667	PF00917	0.530
DOC_USP7_MATH_1	716	720	PF00917	0.639
DOC_USP7_UBL2_3	210	214	PF12436	0.531
DOC_USP7_UBL2_3	729	733	PF12436	0.583
DOC_WW_Pin1_4	122	127	PF00397	0.654
DOC_WW_Pin1_4	142	147	PF00397	0.625
DOC_WW_Pin1_4	180	185	PF00397	0.479
DOC_WW_Pin1_4	289	294	PF00397	0.583
DOC_WW_Pin1_4	326	331	PF00397	0.493
DOC_WW_Pin1_4	399	404	PF00397	0.410
DOC_WW_Pin1_4	488	493	PF00397	0.534
DOC_WW_Pin1_4	502	507	PF00397	0.563
LIG_14-3-3_CanoR_1	266	275	PF00244	0.469
LIG_14-3-3_CanoR_1	406	412	PF00244	0.396
LIG_14-3-3_CanoR_1	422	427	PF00244	0.430
LIG_14-3-3_CanoR_1	431	437	PF00244	0.369
LIG_14-3-3_CanoR_1	448	454	PF00244	0.528
LIG_14-3-3_CanoR_1	461	470	PF00244	0.575
LIG_14-3-3_CanoR_1	714	723	PF00244	0.525
LIG_AP2alpha_1	495	499	PF02296	0.598
LIG_APCC_ABBA_1	335	340	PF00400	0.399
LIG_BIR_II_1	1	5	PF00653	0.529
LIG_BRCT_BRCA1_1	302	306	PF00533	0.575
LIG_BRCT_BRCA1_1	414	418	PF00533	0.383
LIG_BRCT_BRCA1_2	414	420	PF00533	0.452
LIG_Clathr_ClatBox_1	189	193	PF01394	0.514
LIG_CtBP_PxDLS_1	537	541	PF00389	0.587
LIG_deltaCOP1_diTrp_1	486	495	PF00928	0.477
LIG_eIF4E_1	36	42	PF01652	0.456
LIG_FHA_1	117	123	PF00498	0.539
LIG_FHA_1	185	191	PF00498	0.542
LIG_FHA_1	270	276	PF00498	0.465
LIG_FHA_1	293	299	PF00498	0.615
LIG_FHA_1	364	370	PF00498	0.613
LIG_FHA_1	507	513	PF00498	0.520
LIG_FHA_1	589	595	PF00498	0.576
LIG_FHA_1	599	605	PF00498	0.481
LIG_FHA_1	607	613	PF00498	0.389
LIG_FHA_1	696	702	PF00498	0.623
LIG_FHA_1	716	722	PF00498	0.402
LIG_FHA_1	75	81	PF00498	0.631
LIG_FHA_2	110	116	PF00498	0.554
LIG_FHA_2	160	166	PF00498	0.650
LIG_FHA_2	385	391	PF00498	0.349
LIG_FHA_2	423	429	PF00498	0.498
LIG_FHA_2	601	607	PF00498	0.507
LIG_FHA_2	83	89	PF00498	0.563
LIG_GBD_Chelix_1	227	235	PF00786	0.337
LIG_HCF-1_HBM_1	106	109	PF13415	0.567
LIG_LIR_Apic_2	486	492	PF02991	0.485
LIG_LIR_Gen_1	322	331	PF02991	0.542
LIG_LIR_LC3C_4	187	191	PF02991	0.555
LIG_LIR_Nem_3	25	29	PF02991	0.528
LIG_LIR_Nem_3	322	327	PF02991	0.548
LIG_LIR_Nem_3	494	498	PF02991	0.505
LIG_LIR_Nem_3	556	561	PF02991	0.507
LIG_LIR_Nem_3	72	76	PF02991	0.564
LIG_NRBOX	408	414	PF00104	0.441
LIG_NRBOX	90	96	PF00104	0.691
LIG_PCNA_PIPBox_1	605	614	PF02747	0.565
LIG_Pex14_2	495	499	PF04695	0.598
LIG_Rb_LxCxE_1	393	410	PF01857	0.329
LIG_SH2_CRK	26	30	PF00017	0.527
LIG_SH2_GRB2like	118	121	PF00017	0.577
LIG_SH2_PTP2	73	76	PF00017	0.570
LIG_SH2_SRC	195	198	PF00017	0.477
LIG_SH2_SRC	204	207	PF00017	0.331
LIG_SH2_SRC	338	341	PF00017	0.357
LIG_SH2_STAP1	118	122	PF00017	0.560
LIG_SH2_STAP1	204	208	PF00017	0.373
LIG_SH2_STAP1	338	342	PF00017	0.354
LIG_SH2_STAT3	118	121	PF00017	0.554
LIG_SH2_STAT3	36	39	PF00017	0.574
LIG_SH2_STAT5	109	112	PF00017	0.577
LIG_SH2_STAT5	118	121	PF00017	0.523
LIG_SH2_STAT5	195	198	PF00017	0.494
LIG_SH2_STAT5	73	76	PF00017	0.570
LIG_SH3_1	700	706	PF00018	0.561
LIG_SH3_3	120	126	PF00018	0.706
LIG_SH3_3	262	268	PF00018	0.419
LIG_SH3_3	310	316	PF00018	0.522
LIG_SH3_3	495	501	PF00018	0.522
LIG_SH3_3	700	706	PF00018	0.552
LIG_SUMO_SIM_anti_2	135	140	PF11976	0.531
LIG_SUMO_SIM_anti_2	187	194	PF11976	0.511
LIG_SUMO_SIM_anti_2	366	374	PF11976	0.478
LIG_SUMO_SIM_anti_2	550	556	PF11976	0.527
LIG_SUMO_SIM_par_1	187	194	PF11976	0.514
LIG_SUMO_SIM_par_1	366	374	PF11976	0.478
LIG_SUMO_SIM_par_1	476	483	PF11976	0.554
LIG_SUMO_SIM_par_1	500	505	PF11976	0.575
LIG_TRAF2_1	676	679	PF00917	0.575
LIG_TRAF2_1	79	82	PF00917	0.687
LIG_TRAF2_2	471	476	PF00917	0.502
LIG_TYR_ITIM	71	76	PF00017	0.561
LIG_WW_3	150	154	PF00397	0.661
MOD_CDC14_SPxK_1	150	153	PF00782	0.615
MOD_CDC14_SPxK_1	402	405	PF00782	0.420
MOD_CDK_SPK_2	289	294	PF00069	0.560
MOD_CDK_SPxK_1	147	153	PF00069	0.615
MOD_CDK_SPxK_1	399	405	PF00069	0.414
MOD_CDK_SPxxK_3	399	406	PF00069	0.406
MOD_CK1_1	124	130	PF00069	0.666
MOD_CK1_1	145	151	PF00069	0.582
MOD_CK1_1	166	172	PF00069	0.719
MOD_CK1_1	292	298	PF00069	0.580
MOD_CK1_1	319	325	PF00069	0.538
MOD_CK1_1	360	366	PF00069	0.542
MOD_CK1_1	478	484	PF00069	0.580
MOD_CK1_1	568	574	PF00069	0.523
MOD_CK1_1	64	70	PF00069	0.651
MOD_CK1_1	83	89	PF00069	0.451
MOD_CK2_1	294	300	PF00069	0.585
MOD_CK2_1	394	400	PF00069	0.358
MOD_CK2_1	422	428	PF00069	0.504
MOD_CK2_1	600	606	PF00069	0.510
MOD_CK2_1	76	82	PF00069	0.636
MOD_GlcNHglycan	1	4	PF01048	0.606
MOD_GlcNHglycan	129	132	PF01048	0.628
MOD_GlcNHglycan	165	168	PF01048	0.716
MOD_GlcNHglycan	269	272	PF01048	0.465
MOD_GlcNHglycan	279	283	PF01048	0.560
MOD_GlcNHglycan	359	362	PF01048	0.474
MOD_GlcNHglycan	415	418	PF01048	0.363
MOD_GlcNHglycan	449	452	PF01048	0.582
MOD_GlcNHglycan	570	573	PF01048	0.566
MOD_GlcNHglycan	574	577	PF01048	0.537
MOD_GlcNHglycan	578	581	PF01048	0.482
MOD_GlcNHglycan	657	660	PF01048	0.552
MOD_GlcNHglycan	66	69	PF01048	0.548
MOD_GlcNHglycan	665	668	PF01048	0.791
MOD_GSK3_1	121	128	PF00069	0.612
MOD_GSK3_1	159	166	PF00069	0.784
MOD_GSK3_1	180	187	PF00069	0.589
MOD_GSK3_1	289	296	PF00069	0.614
MOD_GSK3_1	401	408	PF00069	0.385
MOD_GSK3_1	457	464	PF00069	0.685
MOD_GSK3_1	487	494	PF00069	0.592
MOD_GSK3_1	502	509	PF00069	0.501
MOD_GSK3_1	518	525	PF00069	0.534
MOD_GSK3_1	563	570	PF00069	0.583
MOD_GSK3_1	572	579	PF00069	0.529
MOD_GSK3_1	596	603	PF00069	0.550
MOD_GSK3_1	655	662	PF00069	0.486
MOD_GSK3_1	76	83	PF00069	0.633
MOD_LATS_1	459	465	PF00433	0.634
MOD_N-GLC_1	357	362	PF02516	0.479
MOD_N-GLC_1	55	60	PF02516	0.617
MOD_N-GLC_1	716	721	PF02516	0.557
MOD_NEK2_1	269	274	PF00069	0.476
MOD_NEK2_1	394	399	PF00069	0.367
MOD_NEK2_1	412	417	PF00069	0.455
MOD_NEK2_1	487	492	PF00069	0.596
MOD_NEK2_1	5	10	PF00069	0.544
MOD_NEK2_1	598	603	PF00069	0.482
MOD_NEK2_1	650	655	PF00069	0.605
MOD_NEK2_1	722	727	PF00069	0.593
MOD_NEK2_1	76	81	PF00069	0.576
MOD_PIKK_1	527	533	PF00454	0.619
MOD_PIKK_1	563	569	PF00454	0.544
MOD_PIKK_1	678	684	PF00454	0.576
MOD_PK_1	294	300	PF00069	0.555
MOD_PKA_1	405	411	PF00069	0.402
MOD_PKA_2	22	28	PF00069	0.566
MOD_PKA_2	293	299	PF00069	0.553
MOD_PKA_2	405	411	PF00069	0.402
MOD_PKA_2	447	453	PF00069	0.594
MOD_Plk_1	475	481	PF00069	0.565
MOD_Plk_1	716	722	PF00069	0.558
MOD_Plk_2-3	197	203	PF00069	0.380
MOD_Plk_4	132	138	PF00069	0.622
MOD_Plk_4	407	413	PF00069	0.406
MOD_Plk_4	475	481	PF00069	0.558
MOD_Plk_4	600	606	PF00069	0.506
MOD_Plk_4	650	656	PF00069	0.487
MOD_Plk_4	69	75	PF00069	0.565
MOD_Plk_4	717	723	PF00069	0.584
MOD_ProDKin_1	122	128	PF00069	0.655
MOD_ProDKin_1	142	148	PF00069	0.626
MOD_ProDKin_1	180	186	PF00069	0.480
MOD_ProDKin_1	289	295	PF00069	0.582
MOD_ProDKin_1	326	332	PF00069	0.486
MOD_ProDKin_1	399	405	PF00069	0.414
MOD_ProDKin_1	488	494	PF00069	0.527
MOD_ProDKin_1	502	508	PF00069	0.561
MOD_SUMO_for_1	613	616	PF00179	0.527
MOD_SUMO_rev_2	303	312	PF00179	0.554
MOD_SUMO_rev_2	616	622	PF00179	0.522
TRG_DiLeu_BaEn_2	307	313	PF01217	0.549
TRG_DiLeu_BaLyEn_6	365	370	PF01217	0.451
TRG_ENDOCYTIC_2	26	29	PF00928	0.527
TRG_ENDOCYTIC_2	338	341	PF00928	0.357
TRG_ENDOCYTIC_2	558	561	PF00928	0.486
TRG_ENDOCYTIC_2	73	76	PF00928	0.570
TRG_ER_diArg_1	443	445	PF00400	0.475
TRG_ER_diArg_1	533	536	PF00400	0.643
TRG_ER_diArg_1	99	102	PF00400	0.560
TRG_NES_CRM1_1	640	652	PF08389	0.508
TRG_NLS_MonoExtC_3	727	732	PF00514	0.600
TRG_NLS_MonoExtN_4	728	735	PF00514	0.660
TRG_Pf-PMV_PEXEL_1	111	115	PF00026	0.727
TRG_Pf-PMV_PEXEL_1	247	252	PF00026	0.389

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PFG5	Leptomonas seymouri	37%	98%
A0A3Q8IVC2	Leishmania donovani	88%	100%
A4HMV4	Leishmania braziliensis	63%	100%
A4IBH5	Leishmania infantum	88%	100%
E9B6G6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	82%	100%