RNA polymerase-associated protein LEO1

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

RNA polymerase-associated protein LEO1, putative

Species:

Leishmania major

UniProt:

E9AFA9_LEIMA

TriTrypDb:

LmjF.35.2850 , LMJLV39_350035700 , LMJSD75_350035000

Length:

555

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0005654	nucleoplasm	2	2
GO:0005737	cytoplasm	2	2
GO:0008023	transcription elongation factor complex	3	11
GO:0016593	Cdc73/Paf1 complex	4	11
GO:0032991	protein-containing complex	1	11
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2
GO:0140513	nuclear protein-containing complex	2	11

Expansion

Sequence features

E9AFA9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFA9

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006354	DNA-templated transcription elongation	6	11
GO:0006355	regulation of DNA-templated transcription	6	2
GO:0006357	regulation of transcription by RNA polymerase II	7	2
GO:0006368	transcription elongation by RNA polymerase II	7	11
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0009058	biosynthetic process	2	11
GO:0009059	macromolecule biosynthetic process	4	11
GO:0009889	regulation of biosynthetic process	4	2
GO:0009891	positive regulation of biosynthetic process	5	2
GO:0009893	positive regulation of metabolic process	4	2
GO:0009987	cellular process	1	11
GO:0010468	regulation of gene expression	5	2
GO:0010556	regulation of macromolecule biosynthetic process	5	2
GO:0010557	positive regulation of macromolecule biosynthetic process	6	2
GO:0010604	positive regulation of macromolecule metabolic process	5	2
GO:0016070	RNA metabolic process	5	11
GO:0016570	histone modification	5	11
GO:0018130	heterocycle biosynthetic process	4	11
GO:0019219	regulation of nucleobase-containing compound metabolic process	5	2
GO:0019222	regulation of metabolic process	3	2
GO:0019438	aromatic compound biosynthetic process	4	11
GO:0019538	protein metabolic process	3	11
GO:0031323	regulation of cellular metabolic process	4	2
GO:0031325	positive regulation of cellular metabolic process	5	2
GO:0031326	regulation of cellular biosynthetic process	5	2
GO:0031328	positive regulation of cellular biosynthetic process	6	2
GO:0032774	RNA biosynthetic process	5	11
GO:0032784	regulation of DNA-templated transcription elongation	7	2
GO:0032786	positive regulation of DNA-templated transcription, elongation	8	2
GO:0032968	positive regulation of transcription elongation by RNA polymerase II	9	2
GO:0034243	regulation of transcription elongation by RNA polymerase II	8	2
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0034654	nucleobase-containing compound biosynthetic process	4	11
GO:0036211	protein modification process	4	11
GO:0043170	macromolecule metabolic process	3	11
GO:0043412	macromolecule modification	4	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044249	cellular biosynthetic process	3	11
GO:0044271	cellular nitrogen compound biosynthetic process	4	11
GO:0045893	positive regulation of DNA-templated transcription	7	2
GO:0045935	positive regulation of nucleobase-containing compound metabolic process	6	2
GO:0045944	positive regulation of transcription by RNA polymerase II	8	2
GO:0046483	heterocycle metabolic process	3	11
GO:0048518	positive regulation of biological process	3	2
GO:0048522	positive regulation of cellular process	4	2
GO:0050789	regulation of biological process	2	2
GO:0050794	regulation of cellular process	3	2
GO:0051171	regulation of nitrogen compound metabolic process	4	2
GO:0051173	positive regulation of nitrogen compound metabolic process	5	2
GO:0051252	regulation of RNA metabolic process	5	2
GO:0051254	positive regulation of RNA metabolic process	6	2
GO:0060255	regulation of macromolecule metabolic process	4	2
GO:0065007	biological regulation	1	2
GO:0071704	organic substance metabolic process	2	11
GO:0080090	regulation of primary metabolic process	4	2
GO:0090304	nucleic acid metabolic process	4	11
GO:1901360	organic cyclic compound metabolic process	3	11
GO:1901362	organic cyclic compound biosynthetic process	4	11
GO:1901564	organonitrogen compound metabolic process	3	11
GO:1901576	organic substance biosynthetic process	3	11
GO:1902680	positive regulation of RNA biosynthetic process	7	2
GO:1903506	regulation of nucleic acid-templated transcription	7	2
GO:1903508	positive regulation of nucleic acid-templated transcription	8	2
GO:2001141	regulation of RNA biosynthetic process	6	2

Molecular functions
Term	Name	Level	Count
GO:0000993	RNA polymerase II complex binding	5	2
GO:0001098	basal transcription machinery binding	3	2
GO:0001099	basal RNA polymerase II transcription machinery binding	4	2
GO:0005488	binding	1	2
GO:0005515	protein binding	2	2
GO:0019899	enzyme binding	3	2
GO:0043175	RNA polymerase core enzyme binding	5	2
GO:0045309	protein phosphorylated amino acid binding	4	2
GO:0050815	phosphoserine residue binding	5	2
GO:0051219	phosphoprotein binding	3	2
GO:0070063	RNA polymerase binding	4	2
GO:0099122	RNA polymerase II C-terminal domain binding	6	2
GO:1990269	RNA polymerase II C-terminal domain phosphoserine binding	6	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	431	435	PF00656	0.719
CLV_C14_Caspase3-7	440	444	PF00656	0.656
CLV_NRD_NRD_1	291	293	PF00675	0.404
CLV_NRD_NRD_1	318	320	PF00675	0.379
CLV_NRD_NRD_1	342	344	PF00675	0.331
CLV_NRD_NRD_1	383	385	PF00675	0.363
CLV_PCSK_KEX2_1	150	152	PF00082	0.672
CLV_PCSK_KEX2_1	291	293	PF00082	0.368
CLV_PCSK_KEX2_1	341	343	PF00082	0.346
CLV_PCSK_KEX2_1	383	385	PF00082	0.364
CLV_PCSK_KEX2_1	399	401	PF00082	0.539
CLV_PCSK_PC1ET2_1	150	152	PF00082	0.672
CLV_PCSK_PC1ET2_1	341	343	PF00082	0.414
CLV_PCSK_PC1ET2_1	399	401	PF00082	0.680
CLV_PCSK_PC7_1	146	152	PF00082	0.552
CLV_PCSK_PC7_1	338	344	PF00082	0.445
CLV_PCSK_SKI1_1	102	106	PF00082	0.504
CLV_PCSK_SKI1_1	108	112	PF00082	0.480
CLV_PCSK_SKI1_1	192	196	PF00082	0.405
CLV_PCSK_SKI1_1	271	275	PF00082	0.477
CLV_PCSK_SKI1_1	338	342	PF00082	0.337
CLV_PCSK_SKI1_1	348	352	PF00082	0.242
CLV_PCSK_SKI1_1	384	388	PF00082	0.319
CLV_PCSK_SKI1_1	468	472	PF00082	0.776
CLV_PCSK_SKI1_1	487	491	PF00082	0.381
DEG_APCC_DBOX_1	486	494	PF00400	0.462
DOC_CKS1_1	195	200	PF01111	0.404
DOC_CYCLIN_RxL_1	484	491	PF00134	0.399
DOC_CYCLIN_yCln2_LP_2	161	167	PF00134	0.520
DOC_MAPK_gen_1	102	112	PF00069	0.576
DOC_MAPK_gen_1	341	352	PF00069	0.278
DOC_MAPK_gen_1	399	409	PF00069	0.602
DOC_PP1_RVXF_1	214	221	PF00149	0.271
DOC_PP2B_LxvP_1	161	164	PF13499	0.494
DOC_PP4_FxxP_1	308	311	PF00568	0.248
DOC_USP7_MATH_1	155	159	PF00917	0.507
DOC_USP7_MATH_1	412	416	PF00917	0.625
DOC_USP7_MATH_1	485	489	PF00917	0.425
DOC_USP7_MATH_1	499	503	PF00917	0.349
DOC_USP7_UBL2_3	341	345	PF12436	0.335
DOC_USP7_UBL2_3	464	468	PF12436	0.548
DOC_WW_Pin1_4	194	199	PF00397	0.463
DOC_WW_Pin1_4	512	517	PF00397	0.515
DOC_WW_Pin1_4	9	14	PF00397	0.667
LIG_14-3-3_CanoR_1	170	174	PF00244	0.381
LIG_14-3-3_CanoR_1	203	211	PF00244	0.360
LIG_14-3-3_CanoR_1	219	227	PF00244	0.167
LIG_14-3-3_CanoR_1	348	353	PF00244	0.267
LIG_14-3-3_CanoR_1	487	495	PF00244	0.509
LIG_14-3-3_CanoR_1	94	98	PF00244	0.479
LIG_AP2alpha_2	422	424	PF02296	0.710
LIG_APCC_ABBA_1	110	115	PF00400	0.560
LIG_APCC_ABBA_1	87	92	PF00400	0.601
LIG_APCC_ABBAyCdc20_2	170	176	PF00400	0.376
LIG_BIR_II_1	1	5	PF00653	0.566
LIG_BIR_III_3	1	5	PF00653	0.503
LIG_BRCT_BRCA1_1	189	193	PF00533	0.464
LIG_FHA_1	197	203	PF00498	0.270
LIG_FHA_1	224	230	PF00498	0.308
LIG_FHA_1	241	247	PF00498	0.300
LIG_FHA_1	248	254	PF00498	0.352
LIG_FHA_1	272	278	PF00498	0.399
LIG_FHA_1	538	544	PF00498	0.456
LIG_FHA_1	68	74	PF00498	0.565
LIG_FHA_1	9	15	PF00498	0.700
LIG_FHA_2	135	141	PF00498	0.652
LIG_FHA_2	18	24	PF00498	0.670
LIG_FHA_2	265	271	PF00498	0.336
LIG_FHA_2	349	355	PF00498	0.271
LIG_FHA_2	513	519	PF00498	0.584
LIG_GBD_Chelix_1	482	490	PF00786	0.500
LIG_LIR_Apic_2	307	311	PF02991	0.370
LIG_LIR_Apic_2	314	318	PF02991	0.361
LIG_LIR_Gen_1	498	507	PF02991	0.521
LIG_LIR_Gen_1	508	516	PF02991	0.572
LIG_LIR_Nem_3	190	196	PF02991	0.526
LIG_LIR_Nem_3	477	482	PF02991	0.446
LIG_LIR_Nem_3	498	503	PF02991	0.520
LIG_NRBOX	485	491	PF00104	0.439
LIG_PCNA_PIPBox_1	71	80	PF02747	0.393
LIG_PCNA_yPIPBox_3	71	83	PF02747	0.395
LIG_Pex14_2	48	52	PF04695	0.487
LIG_SH2_CRK	479	483	PF00017	0.381
LIG_SH2_SRC	113	116	PF00017	0.548
LIG_SH2_SRC	187	190	PF00017	0.469
LIG_SH2_STAT5	119	122	PF00017	0.555
LIG_SH2_STAT5	187	190	PF00017	0.476
LIG_SH2_STAT5	196	199	PF00017	0.311
LIG_SH3_3	162	168	PF00018	0.519
LIG_SH3_3	176	182	PF00018	0.356
LIG_SH3_3	2	8	PF00018	0.619
LIG_SH3_4	464	471	PF00018	0.504
LIG_SUMO_SIM_anti_2	232	239	PF11976	0.335
LIG_SUMO_SIM_anti_2	96	101	PF11976	0.423
LIG_TRAF2_1	238	241	PF00917	0.384
LIG_WRC_WIRS_1	442	447	PF05994	0.524
MOD_CK1_1	212	218	PF00069	0.321
MOD_CK1_1	221	227	PF00069	0.229
MOD_CK1_1	239	245	PF00069	0.271
MOD_CK1_1	488	494	PF00069	0.443
MOD_CK2_1	134	140	PF00069	0.647
MOD_CK2_1	264	270	PF00069	0.417
MOD_CK2_1	320	326	PF00069	0.384
MOD_CK2_1	348	354	PF00069	0.271
MOD_GlcNHglycan	121	124	PF01048	0.502
MOD_GlcNHglycan	2	5	PF01048	0.732
MOD_GlcNHglycan	369	372	PF01048	0.314
MOD_GlcNHglycan	414	417	PF01048	0.715
MOD_GSK3_1	134	141	PF00069	0.536
MOD_GSK3_1	232	239	PF00069	0.313
MOD_GSK3_1	437	444	PF00069	0.691
MOD_GSK3_1	499	506	PF00069	0.402
MOD_GSK3_1	508	515	PF00069	0.419
MOD_N-GLC_1	394	399	PF02516	0.553
MOD_N-GLC_1	512	517	PF02516	0.522
MOD_NEK2_1	138	143	PF00069	0.528
MOD_NEK2_1	202	207	PF00069	0.322
MOD_NEK2_1	40	45	PF00069	0.655
MOD_NEK2_1	67	72	PF00069	0.523
MOD_NEK2_2	223	228	PF00069	0.271
MOD_OFUCOSY	180	185	PF10250	0.560
MOD_PIKK_1	236	242	PF00454	0.404
MOD_PIKK_1	26	32	PF00454	0.728
MOD_PIKK_1	394	400	PF00454	0.465
MOD_PIKK_1	488	494	PF00454	0.403
MOD_PIKK_1	537	543	PF00454	0.472
MOD_PIKK_1	67	73	PF00454	0.406
MOD_PK_1	320	326	PF00069	0.292
MOD_PKA_2	169	175	PF00069	0.399
MOD_PKA_2	202	208	PF00069	0.412
MOD_PKA_2	218	224	PF00069	0.201
MOD_PKA_2	432	438	PF00069	0.538
MOD_PKA_2	93	99	PF00069	0.500
MOD_Plk_1	139	145	PF00069	0.608
MOD_Plk_1	240	246	PF00069	0.395
MOD_Plk_1	306	312	PF00069	0.394
MOD_Plk_1	40	46	PF00069	0.582
MOD_Plk_2-3	279	285	PF00069	0.335
MOD_Plk_2-3	437	443	PF00069	0.563
MOD_Plk_2-3	93	99	PF00069	0.413
MOD_Plk_4	155	161	PF00069	0.487
MOD_Plk_4	232	238	PF00069	0.373
MOD_Plk_4	505	511	PF00069	0.533
MOD_Plk_4	69	75	PF00069	0.500
MOD_ProDKin_1	194	200	PF00069	0.378
MOD_ProDKin_1	512	518	PF00069	0.516
MOD_ProDKin_1	9	15	PF00069	0.665
MOD_SUMO_for_1	470	473	PF00179	0.631
MOD_SUMO_for_1	543	546	PF00179	0.512
MOD_SUMO_rev_2	98	106	PF00179	0.516
TRG_DiLeu_BaEn_2	188	194	PF01217	0.556
TRG_ENDOCYTIC_2	305	308	PF00928	0.248
TRG_ENDOCYTIC_2	479	482	PF00928	0.387
TRG_ER_diArg_1	291	294	PF00400	0.404
TRG_ER_diArg_1	342	344	PF00400	0.426
TRG_ER_diArg_1	382	384	PF00400	0.356
TRG_ER_diArg_1	400	403	PF00400	0.491
TRG_NLS_Bipartite_1	383	403	PF00514	0.603
TRG_NLS_MonoExtC_3	340	345	PF00514	0.422
TRG_NLS_MonoExtC_3	398	404	PF00514	0.680
TRG_NLS_MonoExtN_4	317	323	PF00514	0.403
TRG_NLS_MonoExtN_4	338	345	PF00514	0.292
TRG_NLS_MonoExtN_4	396	403	PF00514	0.667
TRG_Pf-PMV_PEXEL_1	322	326	PF00026	0.404
TRG_Pf-PMV_PEXEL_1	342	346	PF00026	0.150
TRG_Pf-PMV_PEXEL_1	487	492	PF00026	0.533
TRG_Pf-PMV_PEXEL_1	526	530	PF00026	0.536

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IMF7	Leptomonas seymouri	65%	97%
A0A1X0P5N0	Trypanosomatidae	38%	92%
A0A3Q8IMF9	Leishmania donovani	95%	100%
A0A3R7P349	Trypanosoma rangeli	37%	97%
A4HMV2	Leishmania braziliensis	83%	100%
A4IBH3	Leishmania infantum	96%	100%
C9ZZ69	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	35%	96%
E9B6G4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
V5BD17	Trypanosoma cruzi	37%	92%