AB hydrolase-1 domain-containing protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Alpha/beta hydrolase family, putative

Species:

Leishmania major

UniProt:

E9AFA4_LEIMA

TriTrypDb:

LmjF.35.2800 , LMJLV39_350035200 , LMJSD75_350034500

Length:

400

Annotations

LeishMANIAdb annotations

A catalytically active hydrolase 4 domain associated with a multi-pass membrane domain. Probably recognizes membrane-associated substrates.. Distant Eukaryotic relatives typically have signal-anchors and are luminal

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	11
GO:0110165	cellular anatomical entity	1	11

Expansion

Sequence features

E9AFA4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFA4

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	1
GO:0016787	hydrolase activity	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	134	138	PF00656	0.525
CLV_C14_Caspase3-7	291	295	PF00656	0.462
CLV_C14_Caspase3-7	359	363	PF00656	0.518
CLV_NRD_NRD_1	141	143	PF00675	0.390
CLV_NRD_NRD_1	151	153	PF00675	0.232
CLV_NRD_NRD_1	307	309	PF00675	0.327
CLV_NRD_NRD_1	330	332	PF00675	0.344
CLV_PCSK_FUR_1	139	143	PF00082	0.360
CLV_PCSK_KEX2_1	141	143	PF00082	0.341
CLV_PCSK_SKI1_1	308	312	PF00082	0.316
CLV_PCSK_SKI1_1	332	336	PF00082	0.305
CLV_PCSK_SKI1_1	93	97	PF00082	0.605
DEG_COP1_1	157	166	PF00400	0.606
DEG_Nend_Nbox_1	1	3	PF02207	0.653
DEG_SCF_FBW7_1	302	307	PF00400	0.539
DOC_CKS1_1	301	306	PF01111	0.457
DOC_CYCLIN_RxL_1	328	337	PF00134	0.570
DOC_MAPK_gen_1	152	160	PF00069	0.572
DOC_MAPK_HePTP_8	150	162	PF00069	0.525
DOC_MAPK_MEF2A_6	153	162	PF00069	0.611
DOC_PP1_RVXF_1	219	226	PF00149	0.457
DOC_PP2B_LxvP_1	28	31	PF13499	0.350
DOC_USP7_MATH_1	13	17	PF00917	0.621
DOC_USP7_MATH_1	145	149	PF00917	0.455
DOC_USP7_MATH_1	279	283	PF00917	0.518
DOC_USP7_UBL2_3	305	309	PF12436	0.518
DOC_WW_Pin1_4	300	305	PF00397	0.456
LIG_14-3-3_CanoR_1	142	151	PF00244	0.626
LIG_14-3-3_CanoR_1	287	293	PF00244	0.446
LIG_Actin_WH2_2	61	79	PF00022	0.253
LIG_BRCT_BRCA1_1	394	398	PF00533	0.547
LIG_eIF4E_1	278	284	PF01652	0.539
LIG_FHA_1	102	108	PF00498	0.369
LIG_FHA_1	172	178	PF00498	0.540
LIG_FHA_1	225	231	PF00498	0.501
LIG_FHA_1	301	307	PF00498	0.472
LIG_LIR_Apic_2	300	304	PF02991	0.457
LIG_LIR_Gen_1	110	121	PF02991	0.428
LIG_LIR_Gen_1	286	297	PF02991	0.456
LIG_LIR_Gen_1	385	394	PF02991	0.581
LIG_LIR_Gen_1	62	70	PF02991	0.451
LIG_LIR_LC3C_4	174	177	PF02991	0.548
LIG_LIR_Nem_3	110	116	PF02991	0.389
LIG_LIR_Nem_3	183	189	PF02991	0.474
LIG_LIR_Nem_3	382	387	PF02991	0.558
LIG_LIR_Nem_3	62	68	PF02991	0.393
LIG_SH2_GRB2like	376	379	PF00017	0.510
LIG_SH2_SRC	21	24	PF00017	0.626
LIG_SH2_SRC	387	390	PF00017	0.556
LIG_SH2_STAT3	229	232	PF00017	0.582
LIG_SH2_STAT3	336	339	PF00017	0.495
LIG_SH2_STAT5	189	192	PF00017	0.433
LIG_SH2_STAT5	21	24	PF00017	0.569
LIG_SH2_STAT5	229	232	PF00017	0.502
LIG_SH2_STAT5	247	250	PF00017	0.420
LIG_SH2_STAT5	301	304	PF00017	0.562
LIG_SH2_STAT5	50	53	PF00017	0.391
LIG_SH3_3	76	82	PF00018	0.302
LIG_SUMO_SIM_anti_2	5	11	PF11976	0.602
LIG_SUMO_SIM_par_1	103	108	PF11976	0.295
LIG_UBA3_1	63	71	PF00899	0.302
LIG_WW_1	373	376	PF00397	0.517
MOD_CDK_SPK_2	300	305	PF00069	0.518
MOD_CK1_1	290	296	PF00069	0.441
MOD_CK1_1	300	306	PF00069	0.444
MOD_CK1_1	379	385	PF00069	0.535
MOD_GlcNHglycan	16	19	PF01048	0.349
MOD_GlcNHglycan	381	384	PF01048	0.350
MOD_GlcNHglycan	4	7	PF01048	0.362
MOD_GSK3_1	101	108	PF00069	0.330
MOD_GSK3_1	167	174	PF00069	0.563
MOD_GSK3_1	224	231	PF00069	0.506
MOD_GSK3_1	300	307	PF00069	0.504
MOD_GSK3_1	372	379	PF00069	0.502
MOD_GSK3_1	392	399	PF00069	0.583
MOD_N-GLC_1	14	19	PF02516	0.345
MOD_NEK2_1	197	202	PF00069	0.487
MOD_NEK2_1	261	266	PF00069	0.504
MOD_NEK2_1	285	290	PF00069	0.437
MOD_NEK2_1	345	350	PF00069	0.555
MOD_NEK2_1	59	64	PF00069	0.352
MOD_NEK2_1	83	88	PF00069	0.316
MOD_NEK2_2	323	328	PF00069	0.493
MOD_PIKK_1	107	113	PF00454	0.379
MOD_PIKK_1	228	234	PF00454	0.582
MOD_PK_1	154	160	PF00069	0.525
MOD_PKA_2	143	149	PF00069	0.506
MOD_PKA_2	392	398	PF00069	0.550
MOD_Plk_1	154	160	PF00069	0.520
MOD_Plk_1	181	187	PF00069	0.551
MOD_Plk_1	197	203	PF00069	0.430
MOD_Plk_1	285	291	PF00069	0.437
MOD_Plk_1	345	351	PF00069	0.511
MOD_Plk_4	146	152	PF00069	0.620
MOD_Plk_4	171	177	PF00069	0.555
MOD_Plk_4	279	285	PF00069	0.539
MOD_Plk_4	376	382	PF00069	0.541
MOD_Plk_4	59	65	PF00069	0.348
MOD_ProDKin_1	300	306	PF00069	0.456
MOD_SUMO_rev_2	165	170	PF00179	0.626
TRG_DiLeu_BaEn_1	171	176	PF01217	0.452
TRG_ENDOCYTIC_2	387	390	PF00928	0.578
TRG_ER_diArg_1	139	142	PF00400	0.586

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8Q9	Leptomonas seymouri	63%	100%
A0A0S4JC83	Bodo saltans	30%	93%
A0A1X0P6X8	Trypanosomatidae	35%	99%
A0A3S5H7Z7	Leishmania donovani	94%	100%
A0A422P2Q7	Trypanosoma rangeli	35%	99%
A4HMU6	Leishmania braziliensis	78%	100%
A4IBE6	Leishmania infantum	94%	100%
C9ZZ74	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	32%	95%
E9B6F9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
V5BD21	Trypanosoma cruzi	34%	98%